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#31868315   2019/12/23 To Up

Methodological considerations for the high sensitivity detection of multiple myeloma measurable residual disease.

Recent advances in therapeutic interventions have dramatically improved complete response rates in patients with multiple myeloma (MM). The ability to identify residual myeloma cells (e.g., measurable residual disease [MRD]) can provide valuable information pertaining to patient's depth of response to therapy and risk of relapse. Multiparametric flow cytometry is an excellent technique to monitor MRD and has been demonstrated to correlate with patient outcome post-treatment. To achieve the high sensitivity (one abnormal cell in 10 -10 cells) required for MRD evaluation, millions of cells have to be acquired and conventional immunophenotyping protocols are unable to attain these numbers, indicating the needs for alternative flow cytometric staining procedures. A bulk, "Pre-lysis" method is the consensus approach for staining large number of cells, requires two red blood cell lysis steps, and can adversely affect epitope density. In this study, we tested the "Pooled-tube" and "Dextran Sedimentation" staining procedures and correlated them with the "Pre-lysis" method as potential alternative approaches.
Kah Teong Soh, Joseph D Tario, Theresa E Hahn, Jens Hillengass, Philip L McCarthy, Paul K Wallace

1085 related Products with: Methodological considerations for the high sensitivity detection of multiple myeloma measurable residual disease.

96 tests96 wells (1 kit)200 Cuvette 500 ml 96 wells (1 kit)96 tests

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#28365329   2017/03/30 To Up

Lot-to-lot stability of antibody reagents for flow cytometry.

The fluorescence detected using fluorochrome-labelled monoclonal antibodies depends not only on the abundance of the target antigen, but amongst many other factors also on the effective fluorochrome-to-antibody ratio. The diagnostic approach of the EuroFlow consortium relies on reproducible fluorescence intensities over time. A capture bead system for mouse immunoglobulin light chains was utilized to compare the mean fluorescence intensity of 1323 consecutive antibody lots to the currently used lot of the same monoclonal antibody. In total, 157 different monoclonal antibodies were assessed over seven years. Median relative difference between consecutive lots was 3.8% (range: 0.01% to 164.7%, interquartile range: 1.3% to 10.1%). The relative difference exceeded 20% in 8.8% of all comparisons. FITC labelled monoclonal antibodies (median relative difference: 2.1%) showed a significantly smaller variation between lots than antibodies conjugated to PE (3.5%), PECy7 (3.9%), PerCPCy5.5 (5.8%), APC (5.8%), APCH7 (7.4%), and APCC750 (14.5%). Reagents labelled with Pacific Blue (1.4%), Pacific Orange (2.4%), HV450 (0.7%), and HV500 (1.7%) demonstrated more consistent results compared to conjugates of BV421 (4.1%) and BV510 (16.2%). Additionally, significant differences in lot-to-lot fluorescence stability amongst antibodies labelled with the same fluorochrome were observed between manufacturers. These observations might guide future quality recommendations for the production and application of fluorescence-labelled monoclonal antibodies in multicolor flow cytometry.
Sebastian Böttcher, Vincent H J van der Velden, Neus Villamor, Matthias Ritgen, Juan Flores-Montero, Hugo Murua Escobar, Tomas Kalina, Monika Brüggemann, Georgiana Grigore, Marta Martin-Ayuso, Quentin Lecrevisse, Carlos E Pedreira, Jacques J M van Dongen, Alberto Orfao

1612 related Products with: Lot-to-lot stability of antibody reagents for flow cytometry.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#25828588   2015/03/29 To Up

Expression of TNFα membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients.

To investigate the expression of TNFα membrane-bound receptors: the percentage of cells expressing these receptors and the number of molecules expressed on different immune cell subsets, and to evaluate serum concentrations of soluble TNFα and its receptors (sTNFRI and sTNFRII) in patients with rheumatoid arthritis in acute stage and after response to treatment compared to healthy donors.
Sergey V Sennikov, Alina A Alshevskaya, Nadezhda S Shkaruba, Oksana A Chumasova, Aleksey E Sizikov, Julia A Lopatnikova

2759 related Products with: Expression of TNFα membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients.

2 Pieces/Box4 Membranes/Box10 ug4 Membranes/Box4 Membranes/Box11x10e7 cells4 Membranes/Box96 tests

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#24515767   // To Up

A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.

We have recently demonstrated that classical Hodgkin lymphoma (CHL) can be immunophenotyped by flow cytometry (FC), thus obviating the need for immunohistochemistry in many cases. The previously described nine-color assay, however, cannot be used by laboratories that do not have access to a nine- or ten-color flow cytometer. Therefore, a six-color FC tube was designed employing the following combination: CD64-FITC/CD30-PE/CD40-PeCy5.5/CD20-PECy7/CD95-APC/CD3-APC-H7.
Jonathan R Fromm, Brent L Wood

1785 related Products with: A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.

1 kit1 kit1 kit1 kit1 kit1 kit1 kit

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#22564107   2012/05/07 To Up

Treg lymphocytes in autoimmune uveitis.

To evaluate circulating CD4(+)CD25(+) regulatory T-cell populations in patients with autoimmune uveitis and to assess whether T-regulatory cell populations correlate with clinical features.
Simona Ruggieri, Maria Antonia Frassanito, Rosanna Dammacco, Silvana Guerriero

1907 related Products with: Treg lymphocytes in autoimmune uveitis.

200 200 50 10 100 μg100ug200ul20 1 set (5 x 1 ml)2 Pieces/Box1 mL

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#22338049   // To Up

The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.

We have developed a new statistical diagnostic model that examines the correlation between immunophenotype and clonality as detected by flow cytometry (FC) and histology, defining the diagnostic role of FC in multiple myeloma (MM). The 192 bone marrow samples from patients and control subjects were studied for routine diagnostic analysis of MM; a minimum of 100 plasma cells (PCs) were analyzed for each patient sample. A direct 7- or 8-color method was applied to study the immunophenotype of PCs, utilizing a FACSCanto II (BD Biosciences, San Jose, CA). Samples were labeled with fluorochrome-conjugated monoclonal antibodies (AmCyan, Pac Blue, fluorescein isothiocyanate, phycoerythrin [PE], PECy7, peridinin-chlorophyll protein, allophycocyanin [APC], and APC-Cy7) to the following antigens: CD138, CD81, CD200, CD221, CD45, CD38, CD28, CD19, CD27, CD117, CD38, CD33, CD20, CD56, CD10, and immunoglobulin κ and λ light chains. Among all antigens tested, CD19 and CD27, when applied to our model, resulted in optimal concordance with histology. This model defines the effective diagnostic role FC could have in MM and in the detection of minimal residual disease.
Elisa Cannizzo, Giovanni Carulli, Luigi Del Vecchio, Virginia Ottaviano, Emanuele Bellio, Ezio Zenari, Antonio Azzarà, Mario Petrini, Frederic Preffer

2285 related Products with: The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.

11 kit

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#21713550   2011/06/29 To Up

CD44+/CD24- cells and lymph node metastasis in stage I and II invasive ductal carcinoma of the breast.

The presence of tumor-initiating cells (CD44(+)/CD24(-)) in solid tumors has been reported as a possible cause of cancer metastasis and treatment failure. Nevertheless, little is know about the presence of CD44(+)/CD24(-) cells within the primary tumor and metastasis. The proportion of CD44(+)/CD24(-) cells was analyzed in 40 samples and in 10 lymph node metastases using flow cytometry phenotyping. Anti-human CD326 (EpCam; FITC), anti-human CD227 (MUC-1; FITC), anti-human CD44 (APC), and anti-human CD24 (PE), anti-ABCG2 (PE), and anti-CXCR4 (PeCy7) were used for phenotype analysis. The mean patient age was 60.5 years (range, 33-87 years); mean primary tumor size (pT) was 1.8 cm (0.5-3.5 cm). The Wilcoxon or Kruskal-Wallis test was used for univariate analyses. Logistic regression was used for multivariate analysis. The median percentage of CD44(+)/CD24(-) cells within primary invasive ductal carcinomas (IDC) was 2.7% (range, 0.2-71.2). In lymph node metastases, we observed a mean of 6.1% (range, 0.07-53.7). The percentage of CD44(+)/CD24(-) cells in IDCs was not associated with age, pT, tumor grade and HER2. We observed a significantly enrichment of CD44(+)/CD24(-) and ABCG2(+) cells in ESA(+) cell population in patients with positive lymph nodes (P = 0.02 and P = 0.04, respectively). Our data suggest that metastatic dissemination is associated with an increase in tumor-initiating cells in stage I and II breast cancer.
Daniel Guimarães Tiezzi, Fernando Antonio Mourão Valejo, Heitor Ricardo Cosinski Marana, Hélio Humberto Angotti Carrara, Luciana Benevides, Heriton Marcelo Ribeiro Antonio, Renata Danielle Sicchieri, Cristiane Maria Milanezi, João Santana da Silva, Jurandyr Moreira de Andrade

1070 related Products with: CD44+/CD24- cells and lymph node metastasis in stage I and II invasive ductal carcinoma of the breast.



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#21695775   2011/06/21 To Up

An improved flow cytometric assay for detection and discrimination between malignant cells and atypical mesothelial cells, in serous cavity effusions.

The aim of this study was to evaluate a flow cytometric assay for the detection of malignant effusions.
Nektaria A Kentrou, Nikolaos J Tsagarakis, Konstantina Tzanetou, Maria Damala, Konstantinos A Papadimitriou, Dimitra Skoumi, Aimilia Stratigaki, Nikolaos I Anagnostopoulos, Eleni Malamou-Lada, Pauline Athanassiadou, George Paterakis

1086 related Products with: An improved flow cytometric assay for detection and discrimination between malignant cells and atypical mesothelial cells, in serous cavity effusions.

100 µg100 µg1 mg2 ml96 assays25 assays96 wells (1 kit)1 kit100ul100ug

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