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#36468348   2022/12/05 To Up

Immunoadsorption and plasmapheresis at the ICU - A description of the frequencies and indications in a single center experience and a case report.

Immunologically mediated diseases can lead to severe courses that have to be treated in an intensive care unit. The use of extracorporeal organ support systems (ventilation, ECMO) is common. A therapeutic principle for these diseases is the removal of disease-causing antibodies. This can be done nonspecifically by plasmapheresis or specifically by immune adsorption. While most intensive care units have the facilities for plasmapheresis (membrane plasma filtration), immunoadsorption is much less common. Over a period of 10 years, the numbers of immunoadsorption and plasmapheresis treatments performed in a single center intensive care unit are shown according to their indication (IA: 18 Pts, 58 treatments. PA: 54 Pts, 148 treatments). A case study of a patient with granulomatosis with polyangiitis shows the successful treatment with immunoadsorption. The advantages of immunoadsorption in patients with complex coagulation disorders and a critical clinical picture in terms of SIRS and ARDS are shown.
Sebastian Koball, Johannes Rogge, Silvius Frimmel, Michael Hinz, Steffen Mitzner

1781 related Products with: Immunoadsorption and plasmapheresis at the ICU - A description of the frequencies and indications in a single center experience and a case report.

100.00 ul10 mg25 mg50 ug 1000 tests200ul100 mg10 mg

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#36468287   2022/12/05 To Up

Survey of recurrent diagnostic challenges in breast phyllodes tumours.

Breast phyllodes tumours (PTs) are graded as benign, borderline, or malignant by analysis of multiple histological features. PT grading is often inconsistent, likely due to variation in the weighting of grading criteria by pathologists.
Benjamin Yongcheng Tan, Stephen B Fox, Sunil R Lakhani, Puay Hoon Tan

1013 related Products with: Survey of recurrent diagnostic challenges in breast phyllodes tumours.



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#36463990   2022/12/01 To Up

The prevalence of potential pathogens in ballast water and sediments of oceangoing vessels and implications for management.

Ballast water and sediments can serve as prominent vectors for the widespread dispersal of pathogens between geographically distant areas. However, information regarding the diversity and distribution of the bacterial pathogens in ballast water and sediments is highly limited. In this study, using high-throughput sequencing and quantitative PCR, we investigated the composition and abundance of potential pathogens, and their associations with indicator microorganisms. We accordingly detected 48 potential bacterial pathogens in the assessed ballast water and sediments, among which there were significant differences in the compositions and abundances of pathogenic bacterial communities characterizing ballast water and sediments. Rhodococcus erythropolis, Bacteroides vulgatus, and Vibrio campbellii were identified as predominant pathogens in ballast water, whereas Pseudomonas stutzeri, Mycobacterium paragordonae, and Bacillus anthracis predominated in ballast sediments. Bacteroidetes, Vibrio alginolyticus, Vibrio parahaemolyticus, and Escherichia coli were generally detected with median values of 8.54 × 10-1.22 × 10 gene copies (GC)/100 mL and 1.16 × 10-3.97 × 10 GC/100 g in ballast water and sediments, respectively. Notably, the concentrations of Shigella sp., Staphylococcus aureus, and V. alginolyticus were significantly higher in ballast sediments than in the water. In addition, our findings tend to confirm that the indicator species specified by the International Maritime Organization (IMO) might underestimate the pathogen risk in the ballast water and sediments, as these bacteria were unable to predict the majority of the potential pathogens assessed in this study. In summary, this study provides a comprehensive insight into the spectrum of the potential pathogens that transferred by ship ballast tanks and emphasizes the need for the implementation of IMO convention on ballast sediment management.
Baoyi Lv, Guorong Zhu, Wen Tian, Chong Guo, Xiaolan Lu, Yangchun Han, Tingxuan An, Yuxue Cui, Ting Jiang

2358 related Products with: The prevalence of potential pathogens in ballast water and sediments of oceangoing vessels and implications for management.

1000 tests100ug2.5 mg100ug25 mg500 MG2.5 mg50 ug 10 mg100ul

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#36463236   2022/12/03 To Up

Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.

Induced pluripotent stem cells (iPSCs) can in principle differentiate into any cell of the body, and have revolutionized biomedical research and regenerative medicine. Unlike their human counterparts, mouse iPSCs (miPSCs) are reported to silence transposable elements and prevent transposable element-mediated mutagenesis. Here we apply short-read or Oxford Nanopore Technologies long-read genome sequencing to 38 bulk miPSC lines reprogrammed from 10 parental cell types, and 18 single-cell miPSC clones. While single nucleotide variants and structural variants restricted to miPSCs are rare, we find 83 de novo transposable element insertions, including examples intronic to Brca1 and Dmd. LINE-1 retrotransposons are profoundly hypomethylated in miPSCs, beyond other transposable elements and the genome overall, and harbor alternative protein-coding gene promoters. We show that treatment with the LINE-1 inhibitor lamivudine does not hinder reprogramming and efficiently blocks endogenous retrotransposition, as detected by long-read genome sequencing. These experiments reveal the complete spectrum and potential significance of mutations acquired by miPSCs.
Patricia Gerdes, Sue Mei Lim, Adam D Ewing, Michael R Larcombe, Dorothy Chan, Francisco J Sanchez-Luque, Lucinda Walker, Alexander L Carleton, Cini James, Anja S Knaupp, Patricia E Carreira, Christian M Nefzger, Ryan Lister, Sandra R Richardson, Jose M Polo, Geoffrey J Faulkner

1648 related Products with: Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.

1 x 10^6 cells/vial5 x 10A5 cells/vial100.00 ug125ml0.5 ml250 50 ul2ug200 0.1ml (1mg/ml)10ml

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#36462694   2022/11/30 To Up

Selective separation of monovalent anions by PPy/pTS membrane electrodes in redox transistor electrodialysis.

Selective separation of nitrate over chloride is crucial for eutrophication mitigation and nitrogen resource recovery but remains a challenge due to their similar ionic radius and the same valence. Herein, a polypyrrole membrane electrode (PME) was fabricated by polymerization of pyrrole (Py) and p-toluenesulfonate (pTS), which was used as a working electrode in redox transistor electrodialysis. The anions in the source solution were first incorporated into the PME at reduction potentials and then released to receiving solution at oxidation potentials. Pulse widths and potentials were optimized to maximize the ion separation performance of PME, resulting in the improvement of NO/Cl separation factor up to 6.93. The ion distributions in various depths of PME indicated that both NO and Cl were incorporated into PME at negative potentials. Then, NO was preferentially released from PME at positive potentials, but most Cl was retained. This was ascribed to the high binding energy between Cl and PPy/pTS structure, which was 51.4% higher than that between NO and PPy/pTS structure. Therefore, the higher transport rate of NO in comparison with Cl was achieved, leading to a high NO selectivity over Cl. This work provides a promising avenue for the selective separation of nitrate over chloride, which may contribute to nitrogen resource recycling and reuse.
Jingqiu Sun, Xian Zhang, You Wu, Chengzhi Hu

2080 related Products with: Selective separation of monovalent anions by PPy/pTS membrane electrodes in redox transistor electrodialysis.

4 Membranes/Box4 Membranes/Box4 Membranes/Box2000 pcs4 Membranes/Box100μg4 Membranes/Box4 Membranes/Box4 Membranes/Box2 Pieces/Box4 Membranes/Box

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#36455606   // To Up

25 mm Hg versus 35 mm Hg elastic compression stockings to prevent post-thrombotic syndrome after deep vein thrombosis (CELEST): a randomised, double-blind, non-inferiority trial.

The optimal strength of compression needed to prevent post-thrombotic syndrome (PTS) after a proximal deep vein thrombosis (DVT) is debated. We aimed to assess whether 25 mm Hg elastic compression stockings (ECS) are non-inferior to 35 mm Hg ECS in preventing PTS after a DVT.
Jean-Philippe Galanaud, Céline Genty-Vermorel, Marie-Thérèse Barrellier, François Becker, Violaine Jabbour, Sophie Blaise, Alessandra Bura-Rivière, Alexa Comte, Claire Grange, Herve Guenneguez, Mario Maufus, Pierre Ouvry, Cécile Richaud, Carole Rolland, Jeannot Schmidt, Marie-Antoinette Sevestre, François Verrière, Jean-Luc Bosson,

2502 related Products with: 25 mm Hg versus 35 mm Hg elastic compression stockings to prevent post-thrombotic syndrome after deep vein thrombosis (CELEST): a randomised, double-blind, non-inferiority trial.

1 ml0.1 mg100ug Lyophilized1000 1000 1 Set100ug Lyophilized0.1 mg1 Set1 Set16 Arrays/Slide100ug

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#36450758   2022/11/30 To Up

Using coding and non-coding rare variants to target candidate genes in patients with severe tinnitus.

Tinnitus is the phantom percept of an internal non-verbal set of noises and tones. It is reported by 15% of the population and it is usually associated with hearing and/or brain disorders. The role of structural variants (SVs) in coding and non-coding regions has not been investigated in patients with severe tinnitus. In this study, we performed whole-genome sequencing in 97 unrelated Swedish individuals with chronic tinnitus (TIGER cohort). Rare single nucleotide variants (SNV), large structural variants (LSV), and copy number variations (CNV) were retrieved to perform a gene enrichment analysis in TIGER and in a subgroup of patients with severe tinnitus (SEVTIN, n = 34), according to the tinnitus handicap inventory (THI) scores. An independent exome sequencing dataset of 147 Swedish tinnitus patients was used as a replication cohort (JAGUAR cohort) and population-specific datasets from Sweden (SweGen) and Non-Finish Europeans (NFE) from gnomAD were used as control groups. SEVTIN patients showed a higher prevalence of hyperacusis, hearing loss, and anxiety when they were compared to individuals in the TIGER cohort. We found an enrichment of rare missense variants in 6 and 8 high-constraint genes in SEVTIN and TIGER cohorts, respectively. Of note, an enrichment of missense variants was found in the CACNA1E gene in both SEVTIN and TIGER. We replicated the burden of missense variants in 9 high-constrained genes in the JAGUAR cohort, including the gene NAV2, when data were compared with NFE. Moreover, LSVs in constrained regions overlapping CACNA1E, NAV2, and TMEM132D genes were observed in TIGER and SEVTIN.
Alvaro Gallego-Martinez, Alba Escalera-Balsera, Natalia Trpchevska, Paula Robles-Bolivar, Pablo Roman-Naranjo, Lidia Frejo, Patricia Perez-Carpena, Jan Bulla, Silvano Gallus, Barbara Canlon, Christopher R Cederroth, Jose A Lopez-Escamez

2517 related Products with: Using coding and non-coding rare variants to target candidate genes in patients with severe tinnitus.

50ul (1mg/ml)0.1ml96 wells (1 kit)1 mg0.1ml50ul0.1ml (1.3mg/ml)1 mg0.1ml

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#36449167   // To Up

LINE-1 Retrotransposition Assays in Embryonic Stem Cells.

The ongoing mobilization of active non-long terminal repeat (LTR) retrotransposons continues to impact the genomes of most mammals, including humans and rodents. Non-LTR retrotransposons mobilize using an intermediary RNA and a copy-and-paste mechanism termed retrotransposition. Non-LTR retrotransposons are subdivided into long and short interspersed elements (LINEs and SINEs, respectively), depending on their size and autonomy; while active class 1 LINEs (LINE-1s or L1s) encode the enzymatic machinery required to mobilize in cis, active SINEs use the enzymatic machinery of active LINE-1s to mobilize in trans. The mobilization mechanism used by LINE-1s/SINEs was exploited to develop ingenious plasmid-based retrotransposition assays in cultured cells, which typically exploit a reporter gene that can only be activated after a round of retrotransposition. Retrotransposition assays, in cis or in trans, are instrumental tools to study the biology of mammalian LINE-1s and SINEs. In fact, these and other biochemical/genetic assays were used to uncover that endogenous mammalian LINE-1s/SINEs naturally retrotranspose during early embryonic development. However, embryonic stem cells (ESCs) are typically used as a cellular model in these and other studies interrogating LINE-1/SINE expression/regulation during early embryogenesis. Thus, human and mouse ESCs represent an excellent model to understand how active retrotransposons are regulated and how their activity impacts the germline. Here, we describe robust and quantitative protocols to study human/mouse LINE-1 (in cis) and SINE (in trans) retrotransposition using (human and mice) ESCs. These protocols are designed to study the mobilization of active non-LTR retrotransposons in a cellular physiologically relevant context.
Marta Garcia-Cañadas, Francisco J Sanchez-Luque, Laura Sanchez, Johana Rojas, Jose L Garcia Perez

2309 related Products with: LINE-1 Retrotransposition Assays in Embryonic Stem Cells.

1 mg1x10e7 cells10 ug1x10e7 cells1x10e7 cells1 x 10^6 cells/vial1x10e7 cells5 x 10A5 cells/vial1x10e7 cells96 assays100ug100ug

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