Search results for: Polyconal
#31589045 2019/10/07 To Up
Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model.Limited reports on the use of nanobodies (Nbs) in fluorescence polarization immunoassay (FPIA) aroused us to explore if the small size of Nbs is a drawback for the development of sensitive FPIA to small molecular compounds, particularly since FPIA is a technology strongly dependent on molecular weight. In the present work, three different molecular weight Nbs against 3-phenoxybenzoic acid (3-PBA), an exposure biomarker of pyrethroid insecticides, including bare Nbs (15 kDa), Nbs-Avidin (Nbs-AV, 60 kDa), and Nbs-Alkaline phosphatase (Nbs-AP, 130 kDa) were specifically generated to cover distinct regions on the polarization and molecular weight relationship curve for a fluorescein tracer. In competitive FPIA, similar half-maximal inhibitory concentrations (IC) of 3-PBA of 16.4, 12.2, and 14.8 ng mL were obtained for Nbs, Nbs-AV, and Nbs-AP, respectively, indicating that the size of Nbs in the range tested had no significant effect on the sensitivity of the resulting competitive FPIA. An IC of 20.2 ng mL for an anti-3-PBA polyconal antibody based FPIA further demonstrated the performance of Nbs, which was comparable to that of traditional antibodies in FPIA. Spike-recovery studies showed good and reproducible recovery of 3-PBA in urine samples, demonstrating the applicability of Nb-based FPIA. Overall, our results show that Nb-based FPIA achieves sensitivity levels of FPIA based on conventional antibodies and further indicate that Nb absolutely meets the sensitivity requirement of FPIA.
Yulong Wang, Zhenfeng Li, Bogdan Barnych, Jingqian Huo, Debin Wan, Natalia Vasylieva, Junli Xu, Pan Li, Beibei Liu, Cunzheng Zhang, Bruce D Hammock
1241 related Products with: Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model.96 Tests50 assays900 tests100ug Lyophilized1 kit100ug100ug Lyophilized1 kit 1 G100μg 100 G
#17825237 // To Up
[Preparation and characterization of rabbit antibody against human mast cell carboxypeptidase].To prepare antibody against human mast cell carboxypeptidase (hMC-CP) by using recombinant hMC-CP expressed in E.coli, and to characterize the antibody.
Zhang-quan Chen, Su-hui He, Tian-tian Lu, Tian-wen He, Shao-heng He
2306 related Products with: [Preparation and characterization of rabbit antibody against human mast cell carboxypeptidase].100ul 100ul 100ul 100ul0.1 mg100ug Lyophilized 100ul 100ul100ug Lyophilized 100ul 100ul100μl
#16841720 // To Up
[A case of multicentric Castleman disease showing diffuse cystic change in the lung].A 31-year-old woman was admitted to our hospital because of progressive dyspnea and chest X-ray abnormality. She was given a diagnosis of bronchial asthma 3 years previously. She had received medical treatment, but her dyspnea did not improve. Chest CT showed multiple thin-walled cysts and centrilobular nodules throughout both lungs. Video-assisted thoracoscopic lung biopsy revealed remarkable plasmacytic infiltration in the bronchioles and its surrounding interstitium. Small cystic lesions were detected and with remarkable mural plasmacytic infiltration. The immunohistochemistry showed infiltrated plasmacytes with polyconal characteristics. Her biochemical examinations showed polyclonal hyperimmunoglobulinemia and a high range of serum IL-6. In addition, CT scans showed multiple mediastinal and intraperitoneal lymphadenopathy. From these examinations, she was given a diagnosis of multicentric Castleman disease (MCD) with pulmonary involvement showing diffuse cystic change. This case showed an unusual pattern of MCD with pulmonary involvement. However, we suggest that MCD also should be considered as a differential diagnosis in cases with diffuse lung cystic changes.
Takahito Nei, Takemitsu Oiwa, Yoshinobu Saitoh, Shinji Abe, Takashi Motegi, Jiroh Usuki, Arata Azuma, Shoji Kudoh, Kyouji Hirai, Kiyoshi Koizumi, Shinobu Kunugi, Tomoko Nakayama, Yuh Fukuda
1930 related Products with: [A case of multicentric Castleman disease showing diffuse cystic change in the lung].
#11217434 // To Up
[Monocytic B-cells represent a new cell population that is mainly recruited from unmutated polyconal naive B-cells].Monocytoid B-cells appear as a distinct B-cell population in a number of lymphadenopathies but above all in Piringer's lymphadenopathy. Up until now, their assignment to a recognised B-cell subpopulation has not been conclusively achieved. Immunohistological studies have shown characteristics in common with the tumour cells of hairy cell leukemia and also with so-called splenic marginal zone cells. In order to unequivocally clarify their B-cell differentiation stage we have isolated single monocytoid B-cells from immunostained frozen sections and have analysed their immunoglobulin chain gene rearrangements. In addition we have studied the Ig-expression of monocytoid B-cells at both the RNA and protein levels.
K Stein, M Hummel, P Korbjuhn, H D Foss, I Anagnostopoulos, T Marafioti, H Stein
2605 related Products with: [Monocytic B-cells represent a new cell population that is mainly recruited from unmutated polyconal naive B-cells].0.1ml (1mg/ml) 100ul100.00 ug2 mL 1 kit(s) 100100 ug/vial2 ml96 tests1.00 flask 100ul
#11198386 // To Up
Study of protein profile in the visceral leishmaniasis.A study of the ten proteins of the protein profile in 7 children infected with visceral leishmaniasis leads to obtain an evocating diagram of the protein profile in this disease. In these patients, the authors have found the association of a polyconal hypergammaglobulinaemia, an inflammatory syndrom (increase of orosomucoid, CRP, alpha-1-antitrypsin, decrease of albumin, prealbumin and transferrin), and an haemolytic syndrom (decrease of haptoglobin). Such a diagram may be an supplementary help to establish the diagnosis of visceral leishmaniasis, in some cases when it is difficult to find the parasites.
P Bouree, F Botterel, A Lancon1 Set1 Set1 Set1 Set5001 Set1 Set1 Set1 Set100ug Lyophilized1 Set100 μg
#33853243 // To Up
Immunolocalization of hydrophobin HYDPt-1 from the ectomycorrhizal basidiomycete Pisolithus tinctorius during colonization of Eucalyptus globulus roots.â¢â The immunolocalization of one of the hydrophobins of Pisolithustinctorius (HYDPt-1) is reported. Hydrophobin proteins play key roles in adhesion and aggregation of fungal hyphae, and it is already known that formation of ectomycorrhizas on eucalypt roots enhances the accumulation of hydrophobin mRNAs in the mycelium of Pisolithus tinctorius. â¢â Purification of SDS-insoluble proteins from the mycelium of P. tinctorius showed the presence of a 13 kDa polypeptide with properties of class I hydrophobin. â¢â Polyconal antibodies were raised against a recombinant HYDPt-1 polypeptide, and these were used for immunofluorescence-coupled transmission electron microscopy. â¢â HYDPt-1 is a cell wall protein located at the surface of the hyphae with no preferential accumulation in the fungal cells of the different tissues of the ectomycorrhiza (i.e. extraradical hyphae, mantle or Hartig net).
D Tagu, R De Bellis, R Balestrini, O M H De Vries, G Piccoli, V Stocchi, P Bonfante, F Martin
2464 related Products with: Immunolocalization of hydrophobin HYDPt-1 from the ectomycorrhizal basidiomycete Pisolithus tinctorius during colonization of Eucalyptus globulus roots.100ul 5 G100μl10 mg100ug Lyophilized100ug100ug Lyophilized100ug100ul100ug100ug Lyophilized
#7512647 // To Up
Immunohistochemical analysis of tissues regenerated from within periodontal defects treated with expanded polytetrafluoroethylene membranes.Immunocytochemical analysis was carried out on samples of 5-, 6-, and 9-week old regenerated soft tissue taken from healing periodontal defects treated by guided tissue regeneration using expanded polytetrafluorethylene (ePTFE) membranes. A panel of monoclonal and polyconal antibodies to cytokeratins, vimentin, and collagen was used to label cells and collagen types I, III, and IV. Epithelium was identified in 7 of the 9 samples examined, in addition to mesenchymal cells staining positively for vimentin and co-distribution of collagen types I, III, and IV in all samples. Clinical observations indicated that exposure of the ePTFE membranes during healing was a frequent occurrence, and the presence and quantity of epithelium found within the healing defect beneath the membrane may be related to the extent to which this occurs.
S Pritlove-Carson, R M Palmer, P D Floyd, P M Morgan
2300 related Products with: Immunohistochemical analysis of tissues regenerated from within periodontal defects treated with expanded polytetrafluoroethylene membranes.0.1ml (1mg/ml)2100 extractions4 Membranes/Box1 module0,81 module8
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#2787702 // To Up
Anti-idiotype modulation of the in vitro immune response to hepatitis B surface antigen (HBsAg) following remote infection by hepatitis B virus in man.An antigen-inhibitable Ab-2 that exhibits internal image activity will selectively stimulate the in vitro production of anti-HBs in individuals with remotely established immunity to hepatitis B virus. This response is seen (1) in the absence of a polyconal increase in total IgG, (2) with the F(ab')2 component of the Ab-2, (3) in cultures depleted of T-cells, and (4) in the absence of stimulation by antigen. This observation demonstrates that the Ab-2-mediated stimulation of specific IgG production may be an important regulatory function in man.
T R Cupps, S A Haas-Smith, J L Gerin, J L Tibbles, R C Kennedy
2261 related Products with: Anti-idiotype modulation of the in vitro immune response to hepatitis B surface antigen (HBsAg) following remote infection by hepatitis B virus in man.0.1ml100 μg100ug Lyophilized100ug 100 UG100ug Lyophilized100μg96T25 100ug Lyophilized100 μg
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