Search results for: PreMix
#32419329 2020/05/18 To Up
First Case of Glyphosate Resistance in Weedy Sunflower (Helianthus annuus) Confirmed in Texas.Weedy sunflower (Helianthus annuus L.) is a troublesome weed in row-crop production fields in South Texas. Populations with suspected resistance to glyphosate were evaluated with 1X and 4X rates (1X = 868 g ae ha ) of the herbicide, followed by a dose-response assay of the most-resistant population. Molecular studies were conducted to determine if target-site mechanisms were responsible for resistance in these populations. Additionally, field experiments were conducted at two locations (Somerville and Granger, TX) to evaluate the effectiveness of different tank-mix combinations in controlling naturally infesting glyphosate-resistant (GR) weedy sunflower populations in GR corn.
Vijay Singh, Luke Etheredge, Josh McGinty, Gaylon Morgan, Muthukumar Bagavathiannan
2615 related Products with: First Case of Glyphosate Resistance in Weedy Sunflower (Helianthus annuus) Confirmed in Texas.
#32321963 2020/04/22 To Up
Applying nanotechnology to increase the rumen protection of amino acids in dairy cows.The amino acid requirements of high-production dairy cows represent a challenge to ensuring that their diet is supplied with available dietary resources, and thus supplementation with protected amino acids is necessary to increase their post-ruminal supply. Lysine is often the most limiting amino acid in corn-based diets. The present study proposes the use of lipid nanoparticles as novel rumen-bypass systems and assesses their capability to carry lysine. Solid lipid nanoparticles, nanostructured lipid carriers and multiple lipid nanoparticles were considered and their resistance in a rumen inoculum collected from fistulated cows was assessed. All nanoparticles presented diameters between 200-500 nm and surface charges lower than -30 mV. Lysine encapsulation was achieved in all nanoparticles, and its efficiency ranged from 40 to 90%. Solid lipid nanoparticles composed of arachidic or stearic acids and Tween 60 resisted ruminal digestion for up to 24 h. The nanoparticles were also proven to protect their lysine content from the ruminal microbiota. Based on our findings, the proposed nanoparticles represent promising candidates for rumen-bypass approaches and should be studied further to help improve the current technologies and overcome their limitations.
João Albuquerque, Susana Casal, Ricardo Nuno Mendes de Jorge Páscoa, Ingrid Van Dorpe, António José Mira Fonseca, Ana Rita Jordão Cabrita, Ana Rute Neves, Salette Reis
1116 related Products with: Applying nanotechnology to increase the rumen protection of amino acids in dairy cows.10 0.5 mg1 kit96 wells (1 kit)1 mg25 g500 mg1 mg
#32304335 2020/04/18 To Up
Pharmacokinetics of extended-release ivermectin microspheres after oral administration to healthy pigs.We compared the pharmacokinetics of ivermectin premix and ivermectin microspheres in pigs after single and multiple administration regimes. In the single-dose experiments, 24 piglets were randomly divided into three groups and given ivermectin at 0.3 mg/kg using (a) 1.0% ivermectin administered subcutaneously, (b) 0.25% ivermectin premix orally, and (c) 0.25% ivermectin microspheres orally. In the multiple-dose experiment, 6 pigs in two equal groups received ivermectin premix and microspheres orally at 0.3 mg/kg for 7 consecutive days to monitor the valley plasma levels. The plasma samples were detected by fluorescence high-performance liquid chromatography, and concentration-time data were fitted to a noncompartmental model. After oral administration of ivermectin microspheres at a single dose, the elimination rate constant (Kel), the half-life (t ), the peak time (T ), the mean residence time (MRT), and the peak concentration (C ) were 0.012 ± 0.0031/hr, 59.94 ± 20.18 hr, 9.50 ± 0.93 hr, 55.96 ± 11.40 hr, and 37.75 ± 3.45 ng/ml, respectively. The C of microspheres was not statistically different (p > .05) compared with that of premix groups (39.81 ± 5.83 ng/ml). Moreover, the AUC of the microcapsule groups was increased from 1,129.76 ± 245.62 to 1,607.33 ± 343.35 hr ng/ml compared with the premix groups, and the relative bioavailability increased by an average of 17.53% after oral administration with ivermectin microspheres. Multiple-dose administration also indicated pigs fed with ivermectin microspheres can get a higher minimum steady-state concentration and a longer maintenance time than ivermectin premix.
Ying Xu, Shen Zhang, Yangyang Qiu, Yang Yu, Yunxiao Zhang, Xianhui Huang
1740 related Products with: Pharmacokinetics of extended-release ivermectin microspheres after oral administration to healthy pigs.4800 (in case - Racked - 1 module200 ug1 moduleOne 96-Well Strip Micropl 25 G1 kit100 µg1 module
#32229110 2020/03/27 To Up
Comparison of milk production, intake, and total-tract nutrient digestion in lactating dairy cattle fed diets containing either wheat middlings and urea, commercial fermentation by-product, or rumen-protected soybean meal.The objective of this study was to evaluate the effects of a commercially available fermentation by-product in a diet containing adequate rumen-degradable protein (RDP) on milk performance, intake, and total-tract nutrient digestion in lactating dairy cattle. Primiparous (n = 48) and multiparous (n = 144) lactating dairy cattle were stratified by milk production and randomly allocated into 12 pens containing 4 primiparous and 12 multiparous animals each. Cattle averaged 118 d in milk and 712 kg of body weight at trial start. Treatment diets, on a dry matter (DM) basis, consisted of 42% corn silage, 13% alfalfa hay and silage, 20% grain corn, and 25% protein premix containing either soybean meal, wheat middlings, and urea (SBM+U), soybean meal and fermentation by-product (SBM+F), or soybean meal and rumen-protected soybean meal (RP-SBM). All 3 diets provided a similar level (DM basis) of neutral detergent fiber analyzed using α-amylase and sodium sulfite and corrected for ash content (31%), crude protein (CP, 14.9%), starch (26%), and metabolizable energy (2.7 Mcal/kg), and differed in sources of RDP. The trial consisted of a 2-wk adaptation and covariate period during which all cows were fed the RP-SBM diet and covariate measurements were taken. Pens were then randomly allocated to treatments, and weekly measurements of milk production, intake, body weight, and condition score were taken for 10 wk. All data were analyzed using the Proc Mixed procedure in SAS (SAS Institute Inc., Cary, NC). Increased DM intake was observed for cows fed SBM+F compared with cows fed SBM+U and RP-SBM (28.3 vs. 26.9 and 26.7 kg/d, respectively). Cows fed SBM+F produced more energy-corrected milk (45.3 kg/d) compared with cows fed SBM+U and RP-SBM (43.6 and 43.7 kg/d, respectively). Milk protein yield was also increased in cows fed SBM+F. No differences were observed with body weight or condition score gain throughout the trial. Apparent total-tract digestibility of fiber was decreased in cows fed SBM+F, likely as a result of increased intake. Responses are consistent with previous research in our laboratory that demonstrated a decrease in ruminal CP degradation, leading to an increase in metabolizable protein supply in the small intestine. The fermentation by-product might be useful in diets containing adequate amounts of RDP from soybean meal or alfalfa. The results from this experiment demonstrate beneficial milk performance responses to fermentation by-product when fed with a source of RDP.
S W Fessenden, D A Ross, E Block, M E Van Amburgh
1321 related Products with: Comparison of milk production, intake, and total-tract nutrient digestion in lactating dairy cattle fed diets containing either wheat middlings and urea, commercial fermentation by-product, or rumen-protected soybean meal.1 kit100 mg 5 G
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#32189720 2020/03/05 To Up
Characterization of renewable diesel particulate matter gathered from non-premixed and partially premixed flame burners and from a diesel engine.Particulate matter coming from the combustion of renewable diesel (RD), ultra-low sulfur diesel (ULSD) and a volumetric blend of 30% of RD with ULSD (RD30) were collected and physico-chemically characterized. Soot samples were generated in two flame burner types (non-premixed flame, NPF, and partially premixed flame, PPF) trying to simulate the diffusion and premix regimes found in diesel engines. The impact of both fuel nature and burner type was assessed on soot mass, particle size and morphology, particle nanostructure and surface functional groups. In general, although the results of HRTEM and SMPS suggested that the addition of RD reduced the average particle size and increased the concentration of ultra-fine particles, the mass emission of soot was drastically mitigated regardless of the burner used. The results also suggest that the changes in the chemical characteristics of the soot were slightly more sensitive than the changes in the internal nanostructure of the particles, since the graphitic character (as showed by Raman and infrared analysis) increased as the RD content increased, being stronger for the PPF system. Comparisons between engine soot and flame soot confirmed that the addition of RD into ULSD produced smaller and more carbonized particles. In fact, some engine results were located in between those obtained in PPF and NPF burners, suggesting that both combustion regimes are contributing to soot characteristics in engines. This consistency suggests that a first assessment of the impact of alternative fuels on the characteristics of particulate matter can be conducted through the basic approach offered by laboratory flames, thereby avoiding the costs associated with generating large quantities of fuel and the complexities of in-cylinder physical interactions and engine parameters.
Marlon Cadrazco, Alexander Santamaría, I Cristina Jaramillo, Kamaljeet Kaur, K E Kelly, John R Agudelo
1307 related Products with: Characterization of renewable diesel particulate matter gathered from non-premixed and partially premixed flame burners and from a diesel engine.2.5 mg200ug10 mg50 ug 5 G100ul1,000 tests100ug1 mg1000 tests100ug
#32155451 2020/03/06 To Up
Methods comparison for molecular diagnosis of human herpesvirus 8 infections.Human herpesvirus 8 (HHV-8) virological diagnosis and monitoring relies mainly on real-time PCR.
Manon Corgiat, Vincent Calvez, Anne-Geneviève Marcelin, Aude Jary
1987 related Products with: Methods comparison for molecular diagnosis of human herpesvirus 8 infections.20 ug100ug Lyophilized100ug Lyophilized100 TESTS10 ug 1L100ug Lyophilized5 mg50 ug0.1 mg
#32111302 2020/01/22 To Up
Effect of dietary 25-hydroxycholecalciferol on the sternal mass of meat ducks under different vitamin regimens.Genetic selection and intensive nutrition for increased growth rate in meat-type ducks has resulted in an imbalance between pectorales increment and sternal mass, which is detrimental to productivity and welfare. Reducing body weight and increasing sternal mass probably reverses these adverse effects. Therefore, 2 experiments (Expt.) were conducted to investigate the effects of 25-hydroxycholecalciferol (25-OH-D3), a vitamin D3 metabolites, on sternal mass. In Expt. 1, 512 1-day-old male ducks were randomly assigned to 4 low-nutrient density diets and received following treatments in a 2 × 2 factorial arrangement: (i) NRC or China Agricultural industry standards (NY/T) vitamin premixes and (ii) 0.069 mg/kg 25-HyD in feed or not. At 49 D of age, regardless of 25-OH-D3, NY/T vitamin regimen inhibited bone turnover and consequently increased sternal trabecular bone volume and mineral deposition compared with NRC vitamin premix. Supplementing 25-OH-D3 to NRC but not NY/T vitamin regimen significantly improved sternal microarchitecture and mineral content, which companied by decreased serum bone resorption markers concentration, as well as downregulation of the gene expressions of osteoclast differentiation and activity. In Expt. 2, 256 1-day-old male ducks were fed a standard nutrient density diet contained NRC vitamin premix with 0 or 0.069 mg/kg of 25-OH-D3. Results also showed that 25-OH-D3 treatment significantly improved sternal mineral accumulation and microarchitecture, along with decreasing osteoblast and osteoclast numbers in bone surface, declining serum bone turnover markers levels, and increasing serum Ca concentration. Collectively, these findings indicated that the dietary administration of 25-OH-D3 increased sternal mass in NRC vitamin diet by suppressing bone resorption in 49-day-old meat duck.
H Y Zhang, Q F Zeng, S P Bai, J P Wang, X M Ding, Y Xuan, Z W Su, G S Fraley, B Yao, K Y Zhang
1850 related Products with: Effect of dietary 25-hydroxycholecalciferol on the sternal mass of meat ducks under different vitamin regimens.5 G500 Units 500 ml 2 ml 1 kit(s) 100ug Lyophilized1 ml 1 G0.1 mg 100ul 25 ml Ready-to-use
#32089297 2020/02/20 To Up
Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content.The objective of this study was to evaluate effects of butyrate supplementation on plasma concentration of glucagon-like peptide-2 (GLP-2), apparent total-tract digestibility, and responses to a grain challenge of lactating dairy cows fed diets differing in starch content. Eight Holstein cows averaging 58.6 ± 9.96 d in milk (4 primiparous cows fitted with rumen cannula and 4 multiparous intact cows) were blocked by parity and assigned to one of two 4 × 4 Latin squares balanced for carryover effects with a 2 × 2 factorial arrangement of treatments. Treatments were dietary starch content [20.6 vs. 27.5%, respectively, for low starch (LS) and high starch (HS)] and butyrate supplementation (butyrate vs. control) with 21-d periods. Butyrate was provided as Gustor BP70 WS (Norel, S.A., Madrid, Spain), containing 70% sodium butyrate and 30% fatty acid mixture, at 2% of dietary dry matter (providing butyrate at 1.1% of dietary dry matter), and control premix contained 70% wheat bran and 30% fatty acid mixture. Feeds, orts, and fecal samples were collected from d 17 to 19 to determine apparent total-tract nutrient digestibility. Blood and rumen fluid samples were collected on d 19. The baseline of dry matter intake (DMI) was determined as average DMI from d 17 to 19 for each cow, and cows were feed-restricted at 60% of the baseline DMI on d 20, and a grain challenge was conducted by providing steam-flaked corn grain at 0.6% of body weight, on an as-fed basis, in addition to each treatment diet on d 21, and blood and ruminal fluid samples were collected. The interaction of dietary starch content by butyrate supplementation was significant for plasma GLP-2 concentration, being greater for cows fed butyrate with the HS diet than those fed the other 3 diets. Cows fed butyrate increased n-butyrate concentration in the ruminal fluid and tended to increase dry matter and organic matter digestibility compared with the control. During the grain challenge, rumen endotoxin concentration increased over time and was higher for cows fed the HS diets compared with those fed LS diets. However, response variables related to inflammation were not affected by the grain challenge. However, serum haptoglobin, lipopolysaccharide-binding protein, and serum amyloid-A concentrations were greater for cows fed butyrate with the LS diet, but not for those fed the HS diet. These results indicate that butyrate supplementation may increase plasma GLP-2 concentration for cows fed HS diets, and total-tract digestibility regardless of dietary starch content. However, butyrate supplementation did not mitigate inflammation in this study.
R Fukumori, M Oba, K Izumi, M Otsuka, K Suzuki, S Gondaira, H Higuchi, S Oikawa
2499 related Products with: Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content.250ul96 tests200ul96 assays200ul96 tests200ul200ul25mg
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