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Search results for: Prestain

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#29856979   2018/05/29 To Up

Direct and quantitative electrophoretic detection of covalently closed DNA circles formed by in vitro ligation.

The typical products of enzymatic circularization of DNA, using DNA ligase or recombinase, are covalently closed and mostly relaxed DNA circles. Because they are difficult to analyze on conventional gels, they are often converted to nicked circles prior to electrophoresis. Herein, we present a sensitive and quantitative procedure for directly analyzing ligated closed circle DNA on agarose gels without additional treatments. Specifically, inclusion of GelStar dye in the gel allowed detection of ligated closed circle DNAs, which were likely super-twisted by being intercalated by GelStar, as discrete bands with good separation from linear DNA of the same sizes.
Akiko Mizutani, Masafumi Tanaka

1964 related Products with: Direct and quantitative electrophoretic detection of covalently closed DNA circles formed by in vitro ligation.

100tests48 samples100tests16 Arrays/Slide100 ml.96 tests25 ml.100tests100tests

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#28837527   // To Up

Prestained and Preloaded DMEK Grafts: An Evaluation of Tissue Quality and Stain Retention.

To examine endothelial cell damage and stain retention of prestained preloaded Descemet membrane endothelial keratoplasty (DMEK) grafts.
Dorian A Zeidenweber, Khoa D Tran, Christopher S Sales, Stephen W Wehrer, Michael D Straiko, Mark A Terry

2258 related Products with: Prestained and Preloaded DMEK Grafts: An Evaluation of Tissue Quality and Stain Retention.

10 mg1 ml100ug200ug200ul200 25 mg100ul1000 tests10 mg100 mg

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#26771025   // To Up

Hydrophobicity-induced prestaining for protein detection in polyacrylamide gel electrophoresis.

An AIE fluorescent surfactant has been first used to prestain protein by ultrastrong hydrophobic interaction between fluorescent surfactants and proteins, distinguishing from the most widely used poststaining strategies by employing AIE molecules with weak hydrophobic characteristics. A mixture of proteins with variable molecular weights has been detected.
Zhe Li, Weijiang Guan, Chao Lu, Xi-Rui Zhou, Shi-Zhong Luo, Ying You, Jin Ouyang

2095 related Products with: Hydrophobicity-induced prestaining for protein detection in polyacrylamide gel electrophoresis.

96T21 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set

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#24668852   2014/04/19 To Up

Prestaining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by dansylhydrazine.

A new fluorescent prestaining method for gel-separated glycoproteins in 1D and 2D SDS-PAGE was developed by using dansylhydrazine in this study. The prestained gels could be easily imaged after electrophoresis without any time-consuming steps needed for poststains. As low as 4-8 ng glycoproteins (transferrin, α1-acid glycoprotein) could be selectively detected, which is comparable to that of Pro-Q Emerald 488, one of the most commonly used glycoprotein stain. In addition, a subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis was performed to confirm the specificity of the newly developed method.
Yang Wang, Xuan Zhou, Qing Yu, Yuanmeng Duan, Binbin Huang, Guoying Hong, Ayi Zhou, Litai Jin

1852 related Products with: Prestaining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by dansylhydrazine.

500 G 1KG10 mg 1KG1 mg5 mg1 g100 MG2.5 mgSize: 1 mL 100 mM x 21 mg

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#22585558   // To Up

Sensitivity of prestaining RNA with ethidium bromide before electrophoresis and performance of subsequent northern blots using heterologous DNA probes.

Adding ethidium bromide (EtBr) at low concentrations to RNA samples before running formaldehyde-agarose gels affords the advantages of checking RNA integrity and evaluating the quality of size-separation at any time during electrophoresis or immediately after either electrophoresis or blotted the separated RNA onto the membrane without significantly compromising mobility, transfer, or hybridization. In this study, we systematically examined the factors that affect the sensitivity of RNA prestaining by heating RNA samples that include EtBr before electrophoresis under different denaturation conditions. We also examined the efficiency of the hybridization of EtBr-prestained RNA with heterologous DNA probes. The results showed that the fluorescent intensity of EtBr-prestained RNA was affected not only by the EtBr concentration as previously reported but also by the RNA amount, denaturation time, and denaturation temperature. Prior staining of RNA with 40 μg/mL EtBr significantly decreased the efficiency of Northern blot hybridization with heterologous DNA probes. We propose that to best combine staining sensitivity and the efficiency of Northern blot hybridization with heterologous DNA probes, the concentration of EtBr used to prestain RNA should not exceed 30 μg/mL. The efficiency of the hybridization of EtBr-prestained RNA was affected not only by factors that affect staining sensitivity but also by the type of probe used.
Yun Zhao, Linfang Du, Nianhui Zhang

1698 related Products with: Sensitivity of prestaining RNA with ethidium bromide before electrophoresis and performance of subsequent northern blots using heterologous DNA probes.

10 mg 25 GFor 2 miRNA probes, each 10 mL 5 G10 ml10mg/ml; 5X1.0ml 5 G1 mg10 mg 5 G5 X 1000 U

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#18616346   2008/07/11 To Up

SDS-PAGE of proteins using a chameleon-type of fluorescent prestain.

A new prestaining method for protein SDS-PAGE was developed using the fluorogenic amino-reactive label Py-1. This resulted in one of the fastest, most sensitive, and environmentally friendly protocols available. It is mainly due to the unique optical properties of Py-1, which is blue and virtually nonfluorescent but turns to red and becomes much more strongly fluorescent once it is conjugated to the amino group of a protein. Staining times of 30 min are adequate to visualize subnanogram quantities of proteins because pre-electrophoretic labeling Py-1 does not require the time-consuming steps of washing or fixation of gels. LODs as low as 16 pg of protein are found which is better than the best (commercial) poststains and comparable to the best (commercial) prestains. In addition, prestaining requires marginal amounts of staining solution. The change in electrophoretic mobility and band broadening is at a low level because Py-1 causes a mass shift of 288 Da per bound molecule only. By virtue of the small mass shift it causes, this stain is compatible with mass spectrometric protein analysis even though it acts as a covalent label.
Robert J Meier, Mark-Steven Steiner, Axel Duerkop, Otto S Wolfbeis

2339 related Products with: SDS-PAGE of proteins using a chameleon-type of fluorescent prestain.

3500 1100200 310013100

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