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#34563050   2021/09/13 To Up

Combined Transcriptome and Proteome Analysis of Maize ( L.) Reveals A Complementary Profile in Response to Phosphate Deficiency.

A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize ( L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.
Zhi Nie, Bowen Luo, Xiao Zhang, Ling Wu, Dan Liu, Jialei Guo, Xuan He, Duojiang Gao, Shiqiang Gao, Shibin Gao

1892 related Products with: Combined Transcriptome and Proteome Analysis of Maize ( L.) Reveals A Complementary Profile in Response to Phosphate Deficiency.

1 module1 module1 module1 module1 module1 kit1 module1 module1 module1 module1 module1 module

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#34563046   2021/09/07 To Up

Hemopexin Modulates Expression of Complement Regulatory Proteins in Rat Glomeruli.

In systemic hemolysis and in hematuric forms of kidney injury, the major heme scavenging protein, hemopexin (HPX), becomes depleted, and the glomerular microvasculature (glomeruli) is exposed to high concentrations of unbound heme, which, in addition to causing oxidative injury, can activate complement cascades; thus, compounding extent of injury. It is unknown whether unbound heme can also activate specific complement regulatory proteins that could defend against complement-dependent injury. Isolated rat glomeruli were incubated in media supplemented with HPX-deficient (HPX) or HPX-containing (HPX) sera as a means of achieving different degrees of heme partitioning between incubation media and glomerular cells. Expression of heme oxygenase (HO)-1 and of the complement activation inhibitors, decay-accelerating factor (DAF), CD59, and complement receptor-related gene Y (Crry), was assessed by western blot analysis. Expression of HO-1 and of the GPI-anchored DAF and CD59 proteins increased in isolated glomeruli incubated with HPX sera with no effect on Crry expression. Exogenous heme (hemin) did not further induce DAF but increased Crry expression. HPX modulates heme-mediated induction of complement activation controllers in glomeruli. This effect could be of translational relevance in glomerular injury associated with hematuria.
Maria G Detsika, Elias A Lianos

2840 related Products with: Hemopexin Modulates Expression of Complement Regulatory Proteins in Rat Glomeruli.

1mg1 mg15010050 50 100 UG21022

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#34563043   2021/09/02 To Up

Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers.

Colorectal cancer (CRC) is one of the leading causes of cancer-related death over the world. There is a great need for biomarkers capable of early detection and as targets for treatment. Differential protein expression was investigated with two-dimensional gel electrophoresis (2D-PAGE) followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in CRC patient tissue from (i) the peripheral part of the tumor, (ii) the central part of the tumor as well as from (iii) a non-involved part of the colorectal tissue. The expression patterns of six identified proteins were further evaluated by one-dimensional Western blot (1D-WB) analysis of the CRC tissue. Proteins that were perturbed in expression level in the peripheral or in the central part of the tumor as compared with the non-involved part included , , or , and . These identified markers may have future diagnostic potential or may be novel treatment targets after further evaluation in larger patient cohorts.
Maja Ludvigsen, Louise Thorlacius-Ussing, Henrik Vorum, Mogens Tornby Stender, Ole Thorlacius-Ussing, Bent Honoré

1984 related Products with: Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers.

100ug

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#34562941   2021/08/31 To Up

() as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs).

Vaccination is of paramount importance to global health. With the advent of the more recent pandemics, the urgency to expand the range has become even more evident. However, the potential limited availability and affordability of vaccines to resource low- and middle-income countries has created a need for solutions that will ensure cost-effective vaccine production methods for these countries. () (also known as is one of the most promising candidates for expression of heterologous proteins in vaccines development. It combines the speed and ease of highly efficient prokaryotic platforms with some key capabilities of mammalian systems, potentially reducing manufacturing costs. This review will examine the latest developments in from cell engineering and design to industrial production systems with focus on vaccine development and with reference to specific key case studies.
Salomé de Sá Magalhães, Eli Keshavarz-Moore

1060 related Products with: () as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs).

100 assays96 Tests50 assays100Tests1,000 tests100 tests10 mg25 µg200 mg1 LITRE

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#34562940   2021/08/27 To Up

Improved Repopulation Efficacy of Decellularized Small Diameter Vascular Grafts Utilizing the Cord Blood Platelet Lysate.

The development of functional bioengineered small-diameter vascular grafts (SDVGs), represents a major challenge of tissue engineering. This study aimed to evaluate the repopulation efficacy of biological vessels, utilizing the cord blood platelet lysate (CBPL).
Panagiotis Mallis, Dimitrios P Sokolis, Michalis Katsimpoulas, Alkiviadis Kostakis, Catherine Stavropoulos-Giokas, Efstathios Michalopoulos

2578 related Products with: Improved Repopulation Efficacy of Decellularized Small Diameter Vascular Grafts Utilizing the Cord Blood Platelet Lysate.

Unit1.00 flask1ml15ml96T100 µg

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#34562930   2021/09/15 To Up

FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor.

The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypHerExtra is a protein created by fusing the previously described pH sensor SypHer3s with the neurexin transmembrane domain that targets its expression to the cytoplasmic membrane. We showed that with excitation at 445 nm, the fluorescence lifetime of both SypHer3s and SypHerExtra strongly depend on pH. Using FLIM microscopy in live eukaryotic cells, we demonstrated that SypHerExtra can be successfully used to determine extracellular pH, while SypHer3s can be applied to measure intracellular pH. Thus, these two sensors are suitable for quantitative measurements using the FLIM method, to determine intracellular and extracellular pH in a range from pH 7.5 to 9.5 in different biological systems.
Alexander S Goryashchenko, Alexey A Pakhomov, Anastasia V Ryabova, Igor D Romanishkin, Eugene G Maksimov, Alexander N Orsa, Oxana V Serova, Andrey A Mozhaev, Margarita A Maksimova, Vladimir I Martynov, Alexander G Petrenko, Igor E Deyev

1614 related Products with: FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor.

Two 96-Well Microplate KiOne 96-Well Microplate Ki100ugOne 96-Well Microplate Ki50 ug One 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate Ki50 ug One 96-Well Microplate Ki500 testsOne 96-Well Microplate Ki

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#34562843   2021/09/22 To Up

Epigallocatechin gallate (EGCG) attenuates staphylococcal alpha-hemolysin (Hla)-induced NLRP3 inflammasome activation via ROS-MAPK pathways and EGCG-Hla interactions.

Alpha-hemolysin (Hla), the virulence factor secreted by Staphylococcus aureus (S. aureus), plays a critical role in infection and inflammation, which is a severe health burden worldwide. Therefore, it is necessary to develop a drug against Hla. Epigallocatechin gallate (EGCG), a polyphenol extracted from green tea, has excellent anti-inflammatory activity. In this study, we investigated the inhibitory effect of EGCG on Hla-induced NLRP3 inflammasome activation in vitro and in vivo and elucidated the potential molecular mechanism. We found that EGCG attenuated the hemolysis of Hla by inhibiting its secretion. Besides, EGCG significantly decreased overproduction of ROS and activation of MAPK signaling pathway induced by Hla, thereby markedly attenuating the expression of NLRP3 inflammasome-related proteins in THP-1 cells. Notably, EGCG could spontaneously bind to Hla with affinity constant of 1.71 × 10 M, thus blocking the formation of the Hla heptamer. Moreover, Hla-induced expression of NLRP3, ASC and caspase-1 protein and generation of IL-1β and IL-18 in the damaged liver tissue of mice were also significantly suppressed by EGCG in a dose-dependent manner. Collectively, EGCG could be a promising candidate for alleviating Hla-induced the activation of NLRP3 inflammasome, depending on ROS mediated MAPK signaling pathway, and inhibition of Hla secretion and heptamer formation. These findings will enlighten the applications of EGCG to reduce the S. aureus infection by targeting Hla in food and related pharmaceutical fields.
Chunmei Liu, Kun Hao, Zuojia Liu, Zonghui Liu, Na Guo

1405 related Products with: Epigallocatechin gallate (EGCG) attenuates staphylococcal alpha-hemolysin (Hla)-induced NLRP3 inflammasome activation via ROS-MAPK pathways and EGCG-Hla interactions.

1 mg100ug100μg100ug100μg50μg/vial10μg/vial 5 G100ug5 Plates1000pcs 100ul

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#34562831   2021/09/14 To Up

The photo-thermochemical properties and functions of Marchantia phototropin encoded by an unduplicated gene in land plant evolution.

Phototropin (phot) is a blue light photoreceptor in plants and possesses two photosensory light‑oxygen-voltage (LOV1 and LOV2) domains with different photo-thermochemical properties. While liverworts contain a single copy of PHOT (e.g., MpPHOT in Marchantia polymorpha), many land plant species contain multicopy PHOT genes (e.g., AtPHOT1 and 2 in Arabidopsis thaliana) due to evolutionary gene duplication. The LOV domains of duplicated phot proteins have been studied in detail, but those of single-copy phot proteins remain to be characterized. As phot has not been duplicated in liverworts, we hypothesized that Mpphot may retain the ancestral function and photo-thermochemical properties. To learn more about the unduplicated phot proteins, we analyzed chloroplast relocation movement and the photo-thermochemical properties of LOV1 and LOV2 in Mpphot (Mpphot-LOV1 and Mpphot-LOV2, respectively). The function of Mpphot-LOV1, which induced a response to move chloroplasts to weak light (the accumulation response) in the absence of photoactive LOV2, differed from that of LOV1 of the duplicated phot proteins of A. thaliana (e.g., Atphot1-LOV1 preventing the accumulation response). On the other hand, the function of Mpphot-LOV2 was similar to that of LOV2 of the duplicated phots. The photo-thermochemical properties of Mpphot were a hybrid of those of the duplicated phots; the photochemical and thermochemical reactions of Mpphot were similar to those of the phot2- and phot1-type proteins, respectively. Our findings reveal conservation and diversification among LOV domains during phot duplication events in land plant evolution.
Shota Kato, Yamato Takahashi, Yuta Fujii, Kotoko Sasaki, Satoyuki Hirano, Koji Okajima, Yutaka Kodama

1385 related Products with: The photo-thermochemical properties and functions of Marchantia phototropin encoded by an unduplicated gene in land plant evolution.

25UG96T100 μg100ug1 Set

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