Search results for: Rabbit Anti-Acinus Polyclonal Antibody, Cy3 Conjugated
#35518307 
2020/05/18
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A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.
Platelet-derived growth factor BB (PDGF-BB) is a potential biomarker of tumor angiogenesis. For the first time, we developed a highly sensitive aptasensor for PDGF-BB with an enhanced test line signal by using two different gold nanoparticles (AuNPs). Herein, we describe a highly sensitive biosensor for PDGF-BB detection that combines biotinylated aptamer on a sample pad and poly thymine-Cy3-AuNP-monoclonal antibody complexes against PDGF-BB immobilized on conjugate pad A. Streptavidin (SA) and rabbit anti-mouse polyclonal antibody were also immobilized in the nitrocellulose membrane at the test and control zones, respectively. When the target PDGF-BB protein was added, it first bound the aptamer, and later the monoclonal antibody to form a biotinylated complex that was captured by SA, resulting in a visual red line on the test zone. In addition, to enhance the sensitivity, another monoclonal antibody against Cy3 was conjugated on AuNP B and immobilized on conjugate pad B to form a AuNPs (A&B)-antibody-(PDGF-BB-Cy3)-aptamer-biotin-SA complex on the test line when a loading buffer was subsequently added. This approach showed a linear response to PDGF-BB from 3 ng mL to 300 ng mL with a limit of detection as low as 1 ng mL obtained in 10 minutes. Our biosensor displayed results through red lines readable by the naked eye. Interestingly, our approach has been successfully applied for real sample verification, proving its applicability for cancer monitoring and diagnosis.Na Cheng, Yujie Liu, Omar Mukama, Xiaobo Han, Hualin Huang, Shuai Li, Peng Zhou, Xuewen Lu, Zhiyuan Li
1956 related Products with: A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.
100ug Lyophilized100ug Lyophilized50 assays100tests96 tests1 kit1 LITRE100ug Lyophilized100ug Lyophilized0.1 mg
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#27044589 2016/04/05
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Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica).
Cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) act as neurotransmitters and neuromodulators in both the central and peripheral nervous systems. Their presence has been found in different taxonomic groups, in particular in mammals. However, only few investigators have studied these neuropeptides in the class Aves (birds). The aim of the present study was to describe the distribution of CART, NPY and GAL in the pterygopalatine ganglion (PPG) of the domestic duck (Anas platyrhynchos f. domestica).Malgorzata Radzimirska, Krystyna Bogus-Nowakowska, Tadeusz Kuder, Anna Robak