Search results for: Rabbit Anti-BZW2 Polyclonal Antibody
#34119770 2021/05/25 To Up
Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.DNA (cytosine-5)-methyltransferase1 (DNMT1) is the most abundant DNA methyltransferase in somatic cells, and it plays an important role in the initiation, occurrence, and rehabilitation of tumors. Herein, we developed a novel strategy for the detection of the level of DNMT1 in human plasma using the self-assembled nucleic acid probe signal amplification technology. In this method, the DNMT1 monoclonal antibody (McAb) was immobilized on carboxyl magnetic beads to form immunomagnetic beads and then captured DNMT1 specifically. After that, DNMT1 polyclonal antibody (PcAb) and biotinylated sheep anti-rabbit IgG (sheep anti rabbit IgG-Biotin) were sequentially added into the system to react with DNMT1 and form biotinylated double antibody sandwich immunomagnetic beads. In the presence of the bridging medium streptavidin, the biotinylated double antibody sandwich immunomagnetic beads would form a complex with biotinylated poly-fluorescein (Biotin-poly FAM), and the fluorescence intensity of the complex was proportional to the concentration of DNMT1. Immunomagnetic beads can capture the target DNMT1 in the sample, and Biotin-poly FAM can realize signal amplification. Using these strategies, we got a linear range of the system for DNMT1 level detection was from 2Â nmol/L to 200Â nmol/L, and the limit of detection (LOD) was 0.05Â nmol/L. The method was successfully applied for the determination of DNMT1 in human plasma with the recovery of 101.3-106.0%. Therefore, this method has the potential for the detection of DNMT1 level in clinical diagnosis.
Zhenzhen Wan, Fangfang Gong, Mimi Zhang, Leiliang He, Yilin Wang, Songcheng Yu, Jie Liu, Yuming Wu, Li'e Liu, Yongjun Wu, Lingbo Qu, Jiaqi Sun, Fei Yu
2698 related Products with: Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.2 Pieces/Box2 Pieces/Box12 Pieces/Box8 inhibitors100 μg
#34068345 2021/05/13 To Up
Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy.Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs â¤ 6.9% and â¤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any "matrix-effect" on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.
Georgios Koukouvinos, Chrysoula-Evangelia Karachaliou, Ioannis Raptis, Panagiota Petrou, Evangelia Livaniou, Sotirios Kakabakos
2312 related Products with: Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy.100ug100 μg100ug100 100 μg
#34065481 2021/05/20 To Up
An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma.Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of rabbit polyclonal antibody against HE4, covalently attached to a gold chip via cysteamine linker. The biosensor is used with the non-fluidic array SPRi technique. The linear range of the analytical signal response was found to be 2-120 pM, and the biosensor can be used for the determination of the HE4 marker in the plasma of both healthy subjects and ovarian cancer patients after suitable dilution with a PBS buffer. Precision (6-10%) and recovery (101.8-103.5%) were found to be acceptable, and the LOD was equal to 2 pM. The biosensor was validated by the parallel determination of a series of plasma samples from ovarian cancer patients using the Elecsys HE4 test and the developed biosensor, with a good agreement of the results (a Pearson coefficient of 0.989). An example of the diagnostic application of the developed biosensor is given-the influence of ovarian tumor resection on the level of HE4 in blood serum.
Beata Szymanska, Zenon Lukaszewski, Beata Zelazowska-Rutkowska, Kinga Hermanowicz-Szamatowicz, Ewa Gorodkiewicz
1621 related Products with: An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma.1 kit(96 Wells)96T200 100 μg100 μg
#34025984 2021/05/14 To Up
Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties.Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties.
Jing Jin, Kexin Gan, Lan Zhao, Huijuan Jia, Yifan Zhu, Xiongwei Li, Zhaowei Yang, Zhengwen Ye, Ke Cao, Zhiqiang Wang, Mingliang Yu, Yuyan Zhang, Zhisheng Ma, Hangkong Liu, Pere ArÃºs, Jaap H Akkerdaas, Zhongshan Gao, Ronald van Ree
1832 related Products with: Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties.100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
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#34021521 2021/05/21 To Up
A comparative study of human-and rhesus-specific antithymocyte globulins in Rhesus macaques.Rabbit antithymocyte globulin (RATG) preparations are widely used in transplantation. They are developed in vivo against thymocytes and contain polyclonal antibodies specific for myriad cellular targets. The rhesus monkey is commonly used as a preclinical transplant model, but the fidelity of commercially available human-specific RATGs to anticipate the effects of RATGs in rhesus has not been established. We therefore developed two rhesus-specific ATGs (rhATG) and compared them to human-specific RATG (huATG, Thymoglobulin ) in rhesus monkeys, assessing the magnitude and phenotype of depletion peripherally and in lymph nodes. Four primates were assigned to each group and received 20Â mg/kg of drug. Depletion, repopulation, and changes in lymphocyte subsets were evaluated in peripheral blood and lymph nodes by flow cytometry over four months. We observed similar qualitative changes in lymphocyte subsets, but a generally more profound depletion with huATG compared to either rhATG. Peripheral homeostatic proliferation rather than thymic output was the major mechanism for repopulation with all RATGs. Repopulation was slower but qualitatively similar when examining RATGs in additional animals receiving concomitant chronic immunosuppression. Depletional induction is similar to human- and rhesus-specific RATGs in rhesus macaques. Both rhesus- and human-specific agents appear appropriate for preclinical modeling of clinical RATG use.
Brian I Shaw, Robin Schmitz, Walter J Flores, Diogo M Magnani, Jie Li, Mingqing Song, Allan D Kirk
1568 related Products with: A comparative study of human-and rhesus-specific antithymocyte globulins in Rhesus macaques.4 Membranes/Box4 Membranes/Box4 Arrays/Slide4 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box4 Membranes/Box100 µg4 Arrays/Slide4 Membranes/Box4 Membranes/Box
#33977321 2021/05/11 To Up
Production of polyclonal antibody against the recombinant PirB protein of Vibrio parahaemolyticus.Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA and PirB encoded in pVA1 plasmid. The polyclonal antibodies against PirB protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.
Ngoc-Diem Duong, Khai-Hoan Nguyen-Phuoc, Kim-Yen Thi Do, Nguyet-Thu Thi Nguyen, Thuoc Linh Tran, Hieu Tran-Van
2305 related Products with: Production of polyclonal antibody against the recombinant PirB protein of Vibrio parahaemolyticus.100ug Lyophilized100ug100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
#33974477 2021/05/11 To Up
Motilin Receptor Expression Found in the Human Main and Accessory Lacrimal Glands.: In this study, we investigated the presence of motilin receptors (MR) in adnexal tissue including the human main lacrimal gland.: 17 adnexal human specimens comprising of 11 isolated human main lacrimal gland specimens, four full-thickness human eyelid excisions and two exenterations containing full-thickness eyelid and portions of the main lacrimal gland were immunolabelled with a rabbit polyclonal human MR antibody.: Our results demonstrated that all main lacrimal gland specimens (13/13, 100%) were positive for MR expression with a predominance (10/13 (77%) of grade 1+ punctate distribution. Motilin receptors were not found in eccrine glands, cutaneous sebaceous glands, glands of Zeis or glands of Moll (0/6, 0%). We also confirmed MR expression in the accessory lacrimal gland tissue.: In summary, we discovered the MR receptor in the lacrimal and accessory lacrimal gland - the significance of which, in the lacrimal gland, remains unclear - but motilin may play a role in the muscarinic control of aqueous tear secretion.
Richard R Sadig, Alexandra Allende, Geoffrey Hall, Dinh Tran, Michele C Madigan, Stephanie L Watson, Kenneth G-J Ooi
1317 related Products with: Motilin Receptor Expression Found in the Human Main and Accessory Lacrimal Glands.0.1 mg100 μg100 μg100 μg100 μg50 ul96 wells (1 kit) 100ul1000 96T1 ml100ul
#33968077 2021/04/23 To Up
A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1.Fibrinogen-like protein 1 (FGL1), a member of the fibrinogen family, is a specific hepatocyte mitogen. Recently, it has been reported that FGL1 is the main inhibitory ligand of lymphocyte activating gene 3 (LAG3). Furthermore, the FGL1-LAG3 pathway has a synergistic effect with programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway and is regarded as a promising immunotherapeutic target. However, swine FGL1 (sFGL1) has not been characterized and its detection method is lacking. In the study, the sFGL1 gene was amplified from the liver tissue of swine and then inserted into a prokaryotic expression vector, pQE-30. The recombinant plasmid pQE30-sFGL1 was transformed into JM109 competent cells. The recombinant sFGL1 was induced expression by isopropyl-Î²-d-thiogalactoside (IPTG) and the purified sFGL1 was used as an antigen to produce mouse monoclonal antibody (mAb) and rabbit polyclonal antibody (pAb). After identification, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for sensitive and specific detection of sFGL1 was developed. Swine FGL1 in samples was captured by anti-sFGL1 mAb followed by detection with anti-sFGL1 rabbit pAb and HRP-conjugated goat anti-rabbit IgG. The limit of detection of the developed sFLG1-DAS-ELISA is 35 pg/ml with recombinant sFLG1. Besides, it does not show cross-reactivity with the control protein. Then serum samples of PRRSV-negative and -positive pigs were tested with the established DAS-ELISA and calculated according to the equation of y=0.0735x+0.0737. The results showed that PRRSV infection enhanced the serum FGL1 levels significantly. Our research provides a platform for the research on the functional roles of swine FGL1.
Xin Zhang, Haipeng Zhu, Xu Zheng, Yunjie Jiao, Lulu Ning, En-Min Zhou, Yang Mu
2893 related Products with: A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1.1 kit0.1 mg25 µg100ug Lyophilized100 ml100 TESTS100ug100μg100ug Lyophilized0.1 ml0.1 mg96 tests
#33960377 2021/05/07 To Up
Detection of four quinolone antibiotic by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.A simple and effective direct competitive chemiluminescence immunoassay for detection of four kind of quinolone antibiotics in milk was established using Nor-Biotin(biotin-modified Norfloxacin) bifunctional ligand and SA-ALP signal amplification technology. The polyclonal antibody was obtained after immunization of New Zealand White rabbits using Norfloxacin-derived antigen. "Click chemistry" was used for the rapid and facile synthesis of the Nor-Biotin bifunctional ligand. After the optimization of the incubation time and reaction buffer, the direct competitive ChemiLuminescence assay (dcCLIA) method was developed and used for sensitive detection of four kinds of quinolone drugs (norfloxacin (NOR), pefloxacin (PFLX), ciprofloxacin (CIP), and danofloxacin (DFLX)). The IC50 of the four kinds of quinolone drugs ranged from 7.35 ng/mL to 24.27 ng/mL, and the lowest detection limits (LOD) ranged from 0.05 ng/mL to 0.16 ng/mL, which were below their maximum residue levels (MRLs), approved by the EU for treatment of food-producing animals. To demonstrate the applicability of the assay, artificially contaminated milk samples with the four-quinolone drugs were analyzed. The mean recovery rates of the drugs ranged from 86.31% to 112.11%.
Zhenyu Han, Tieqiang Sun, Zehua Xu, Longxing Fan, Hanxuan Yun, Xuejiao Ge, Xiao Liu, Ying Liu, Bao'an Ning
2908 related Products with: Detection of four quinolone antibiotic by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.50 0.2 mg50 100ug100ug0.25 mg50 100ug100ug0.5 mg50 100ug
#33944773 2021/05/03 To Up
Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella.Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. This parasite encodes a genome of more than 8000 genes. However, more than 70% of the gene models for this species are currently annotated as hypothetical proteins. In this study, a conserved hypothetical protein gene of E. tenella, designated EtCHP18905, was cloned and identified, and its immune protective effects were evaluated. The open reading frame of EtCHP18905 was 1053bp and encoded a protein of 350 amino acids with a molecular weight of 38.7kDa. The recombinant EtCHP18905 protein (rEtCHP18905) was expressed in E. coli. Using western blot, the recombinant protein was successfully recognized by anti GST-Tag monoclonal antibody and anti-sporozoites protein rabbit serum. Real-time quantitative PCR analysis revealed that the EtCHP18905 mRNA levels were higher in sporozoites than in unsporulated oocysts, sporulated oocysts and second-generation merozoites. Western blot analysis showed that EtCHP18905 protein expression levels were lower in sporozoites than in other stages. Immunofluorescence analysis indicated that the EtCHP18905 protein was located on the surface of sporozoites and second-generation merozoites. Inhibition experiments showed that the ability of sporozoites to invade host cells was significantly decreased after treatment with the anti-rEtCHP18905 polyclonal antibody. Vaccination with rEtCHP18905 protein was able to significantly decrease mean lesion scores and oocyst outputs as compared to non-vaccinated controls. The results suggest that the rEtCHP18905 protein can induce partial immune protection against infection with E. tenella and could be an effective candidate for the development of new vaccines.
Huanzhi Zhao, Shunhai Zhu, Qiping Zhao, Bing Huang, Guiling Liu, Zhihang Li, Lu Wang, Hui Dong, Hongyu Han
1261 related Products with: Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella.100ul 100ul 100ul1000 TESTS/0.65ml 100ul100ul10 mg1 Set0.1 mg1 Set100ul10
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