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Search results for: Rabbit Anti-C12ORF53 Polyclonal Antibody, Gold conjugated Isotype: IgG

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#32663533   2020/07/11 To Up

A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.

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Ramasamy Selvarajan, Prasanya Selvam Kanichelvam, Velusamy Balasubramanian, Sundaram Sethurama Subramanian

2956 related Products with: A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.

96 tests100tests1 kit1 kit24 tests0.25 mg

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#32199081   2020/02/24 To Up

Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).

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1335 related Products with: Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).

One 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki500 testsOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki

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#28727765   2017/07/20 To Up

Defining the target and the effect of imatinib on the filarial c-Abl homologue.

Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites.
Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman

1582 related Products with: Defining the target and the effect of imatinib on the filarial c-Abl homologue.

min 2 cartons100.00 ul1 ml1200 units1500 Units11mg

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#27940044   2016/12/08 To Up

Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.

Here, we report the development of an immunochromatographic test strip that can detect heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. Five types of monoclonal antibody (mAb)-producing hybridomas were isolated: three mAbs were A subunit specific and two were B subunit specific. Four mAbs also cross-reacted with both LT proteins derived from swine and human E. coli strains, but only one mAb 57B9 additionally cross-reacted with cholera toxin. Thus, mAb 57B9 was used to form a gold colloid-conjugated antibody for the immunochromatographic test by combination with polyclonal anti-LT rabbit IgG. This test strip detected not only LT in the culture supernatant of LT gene-positive strains, but also cholera toxin in the culture supernatant of Vibrio cholerae. These results indicate that this test strip is suitable for the diagnosis of both enterotoxigenic E. coli and V. cholerae infection.
Hideyuki Arimitsu, Keiko Sasaki, Takao Tsuji

2434 related Products with: Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.

1 mg1 mg0.2 mg0.2 mg 5 G2x384 well plate0.1 mg100 U400 assays

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#19699744   2009/08/21 To Up

Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.

For the application of antibodies in rapid test systems such as Lateral Flow Devices (LFD) antibodies have to be coupled to coloured particles for immediate readability of the test system. In this work colloidal gold was selected for conjugation to the antibodies. Polyclonal rabbit antibodies were chosen for the development of Lateral Flow Devices for the detection of bovine alpha-casein. For antibody comparison chicken egg yolk IgY and sheep IgG were additionally used. Rabbit and chicken antibodies were purified from rabbit sera and egg yolk using affinity chromatography and alternatively ammonium sulphate precipitation, Sheep IgG was commercially obtained. In the course of colloidal gold sol titration experiments differences not only between antibody species but also between differently purified rabbit IgG were observed. While affinity purified rabbit IgG was not able to stabilise colloidal gold particles, antibodies obtained by ammonium sulphate precipitation resulted in a stable gold conjugate suitable for application in Lateral Flow Assays. This work compares and discusses the impact of antibody pre-treatment on further conjugation capacity.
Judith Rudolf, Manuela Führer, Brigitte Galler, Parisa Ansari, Christoph Hasenhindl, Sabine Baumgartner

1814 related Products with: Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.

100ul100ug Lyophilized50 ug 1000 100ug Lyophilized1 ml1000 TESTS/0.65ml50 ug 100ug Lyophilized100.00 ul100ug50 ug

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#10583677   // To Up

Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.

Specific nisin polyclonal antibodies (PAb) were produced in rabbits using nisin Z produced by Lactococcus lactis subsp. lactis biovar diacetylactis UL 719. Antisera were obtained from white female New Zealand rabbits that were first immunized with a nisin Z-keyhole limpet haemocyanin conjugate and boosted with free nisin Z. Nisin-specific PAb were purified by affinity chromatography with a yield of 15 mg specific antinisin 100 ml-1 serum. The detection limit of the ELISA test for nisin Z was 0.75 ng ml-1 in buffer but was 1.7 and 3.5 ng ml-1 in milk and complex media broth spiked (5, 10, 20 microg ml-1) with nisin Z, respectively. In nisin Z-spiked samples, the average concentration was between 90 and 107% of actual added amount. In contrast, when the bioassay (microtitration method) was used, only 50-63% of nisin Z biological activity could be detected. In addition, the affinity-purified nisin PAb, antirabbit IgG gold conjugate and transmission electron microscopy were successfully used to locate nisin Z on producing cells and to observe its bactericidal effects against sensitive cells.
M Bouksaim, C Lacroix, R Bazin, R E Simard

1143 related Products with: Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.

100ug Lyophilized50 ug 50 ug 96T50 ug 100ug Lyophilized100ug Lyophilized96 wells (1 kit)1000 TESTS/0.65ml0.1 mg1 ml0.1ml (1mg/ml)

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