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#17056686   2006/10/20 To Up

Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50.

P
Raquel Criado, Jorge Gutiérrez, María Martín, Carmen Herranz, Pablo E Hernández, Luis M Cintas

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100ug100 mg1000 tests100ul5mg500 MG25 mg10 mg100ug50 mg25 mg2.5 mg

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#1401937   // To Up

The detection of intracytoplasmic interleukin-1 alpha, interleukin-1 beta and tumour necrosis factor alpha expression in human monocytes using two colour immunofluorescence flow cytometry.

T
M P de Caestecker, B A Telfer, I V Hutchinson, F W Ballardie

2858 related Products with: The detection of intracytoplasmic interleukin-1 alpha, interleukin-1 beta and tumour necrosis factor alpha expression in human monocytes using two colour immunofluorescence flow cytometry.

10ug100ug Lyophilized100ug Lyophilized100ug Lyophilized1000 units 100ul 100ul100ug Lyophilized500 96 wells (1 kit) 100ul100ug Lyophilized

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#1712263   // To Up

Concomitant delineation of surface Ig, B-cell differentiation antigens, and HLADR on lymphoid proliferations using three-color immunocytometry.

Accurate and consistent enumeration of B-cell subpopulations in lymphoid tissue was achieved through multiparameter three-color immunofluorescence and flow cytometric analysis (FCM). Phycoerythrin (PE)-anti-CD19 (Leu12) and biotinylated anti-HLADr/streptavidin-Duochrome (PE/Texas Red), used in conjunction with polyclonal fluorescein isothiocyanate (FITC) conjugated anti-surface immunoglobulin (SIg) antibodies, effectively separated non-specific binding and background fluorescence from true B-cell surface FITC immunofluorescence, while concomitantly analyzing for HLADr and CD19 phenotypic expression/deletion. Autofluorescence was measured to establish a fluorescence threshold. A second control measured non-specific binding of isotypic control mouse Ig and non-immune rabbit IgG. Cell suspensions from 128 samples of various lymphoid proliferations were studied. In 116 of the 128 samples, kappa/lambda ratios determined by flow cytometry correlated well with immunocytology results obtained using cytospins from the same cell suspension and with histopathologically established diagnosis. Clonality and lineage as defined immunotypically by flow cytometry was concordant with genotypic results in 64 of the 67 cases evaluated. SIg, HLADr, and CD19 deletions were demonstrated by flow cytometry in 8, 4, and 1 case(s), respectively. Discordance was usually attributable to selective loss of large neoplastic cells in flow cytometry specimens or absent expression of SIg by some cytoplasmic Ig (CIg+) lymphomas.
G H Segal, M G Edinger, M Owen, M McNealis, P Lopez, A Perkins, M D Linden, A J Fishleder, M H Stoler, R R Tubbs

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24 wells100tests24 wells1 kit5 x 50 ug1 kit50 ug100 μg5ug1 kit

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#2826576   // To Up

Distribution of ecto-5'-nucleotidase on subsets of human T and B lymphocytes as detected by indirect immunofluorescence using goat antibodies.

H
L F Thompson, J M Ruedi, M G Low, L T Clement

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100 μg100 μg100 μg100 μg100 μg100 μg0.1 ml100 μg100 μg100 μg100 μg100 μg

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