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Search results for: Rabbit Anti-HECA Polyclonal Antibody, FITC conjugated,Isotype: IgG

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#27617027   2016/09/09 To Up

Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

Retinal ischemia is a retinal disorder related to retinal vascular occlusion, glaucoma, diabetic retinopathy and age-related macular degeneration. The study aimed to evaluate the protective effects and underlying mechanisms of Chi-Ju-Di-Huang-Wan (CJDHW) against retinal ischemia in rats.
Hsiao-Ming Chao, Lei Hu, Ji-Min Cheng, Xiao-Qian Liu, Jorn-Hon Liu, Wynn Hwai-Tzong Pan, Xiu-Mei Zhang

2988 related Products with: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

100ug100 μg2 ml50096T100ug5 100μg1x96 well plate100ul1mg

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#21620854   2011/05/18 To Up

Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

Hypochlorite is a strong oxidant, generated under pathological conditions, with the potency to introduce chlorine atom into a number of molecules. 3-Chloro- and 3,5-dichlorotyrosine are documented to be generated by this oxidant and their elevated levels were found in many diseases. Thus, we decided to check the possibility of use of FITC-conjugated antibodies for flow cytometric detection of 3-chlorotyrosine residues in human cells (A549, MCF-7, HUVEC-ST) exposed to the action of hypochlorite. Additionally, we compared the effects of chlorohydrins and N-chloroamino acids as chlorine donors. Cell fixation and permeabilization was followed by incubation with rabbit polyclonal anti-3-chlorotyrosine primary antibody and subsequent staining with goat anti-rabbit FITC-labeled secondary antibody. For antibody isotypic control, normal rabbit IgG was employed. Hypochlorite appeared to be the most efficient from the chlorocompounds analyzed in chlorotyrozine generation in all cell lines. Statistically significant increase of fluorescence corresponding to the level of 3-chlorotyrosine residues was found in cells treated with hypochlorite even at non-toxic concentrations (<5μM). This effect was not observed in cells exposed to the action of chlorinated amino acids or chlorohydrins. The use of anti-3-chlorotyrosine antibodies in conjunction with fluorophore-conjugated secondary antibodies analysis allows for detection of 3-chlorotyrosine residues by flow cytometry in cells treated with low doses of hypochlorite.
Agnieszka Robaszkiewicz, Grzegorz Bartosz, Miroslaw Soszynski

2333 related Products with: Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

1 kit100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1.00 flask100ug Lyophilized100ug Lyophilized1.00 flask1.00 flask1 mg1.00 flask

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#20807555   2010/08/31 To Up

Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

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Zhouping Wang, Tingting Miu, Huan Xu, Nuo Duan, Xiaoying Ding, Shuang Li

1772 related Products with: Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

96 wells

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#17723519   2006/03/14 To Up

Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

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Kyoko Hibi, Akihisa Abe, Eiji Ohashi, Kohji Mitsubayashi, Hideki Ushio, Tetsuhito Hayashi, Huifeng Ren, Hideaki Endo

2500 related Products with: Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

1 kit200 1 kit1 kit1 kit200 1 kit1 kit 5 G1 kit

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#16035233   // To Up

Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

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Nihal Buzkan, Lászlo Kocsis, M Andrew Walker

2347 related Products with: Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

100ug100ug100 mg1000 tests500 MG100ul25 mg10 mg1000 TESTS/0.65ml50 mg25 mg96T

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#15896799   2005/04/09 To Up

Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

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Christopher M Bearden, Benita K Book, Richard A Sidner, Mark D Pescovitz

2468 related Products with: Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

100 100UG 100UG0.1 mg200 100UG100 Tests1 mg1 mg200 100UG1 mg

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#15020090   // To Up

Dual enhancement of triple immunofluorescence using two antibodies from the same species.

Triple immunofluorescence method with two mouse monoclonal antibodies and another rabbit polyclonal antibody was established with catalyzed reporter deposition (CARD) amplification on thick floating sections from the rat cerebellum. One of the monoclonal antibodies (anti-calbindin), diluted maximally, probed with anti-mouse IgG-horseradish peroxidase (HRP) and amplified with Cy5-conjugated tyramide, immunolabeled cerebellar Purkinje cells and their arborization. Subsequently, a rabbit polyclonal IgG (anti-glial fibrillary acidic protein (anti-GFAP)), probed with anti-rabbit IgG-HRP, amplified with biotin-tyramide and visualized with fluorescein-isothiocyanate (FITC)-streptavidin, immunolabeled Bergmann's glia. Another mouse monoclonal IgG (anti-SNAP25), probed with anti-mouse IgG-rhodamine without CARD amplification, selectively visualized synaptic sites, because the maximal dilution of the other monoclonal antibody (anti-calbindin) was below the detection threshold of this anti-mouse IgG-rhodamine. Separation of the two signals (calbindin and SNAP25), each detected through mouse monoclonal antibody, was then based on the difference of sensitivity either with or without CARD amplification. Triple immunofluorescence is possible when just one of the three primary antibodies is from different species. Intensification of two of the three signals provides further advantages to examine immunolocalization of multiple epitopes on histological sections.
Ayako Nakamura, Toshiki Uchihara

1023 related Products with: Dual enhancement of triple immunofluorescence using two antibodies from the same species.

50 ug Product tipe: Antib50 ug Product tipe: Antib0.1 mg50 ug Product tipe: Antib100 ug Product tipe: Anti100 ul Product tipe: Anti100 μg20 ug Product tipe: Antib1 ml 100ul100

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#12730261   2003/04/28 To Up

In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.

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M M Maryani, M V Morse, G Bradley, H R Irving, D M Cahill, C A Gehring

1069 related Products with: In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.

4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo

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#12046090   // To Up

Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

To observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs.
Jian-Ping Gong, Li-Li Dai, Chang-An Liu, Chuan-Xin Wu, Yu-Jun Shi, Sheng-Wei Li, Xu-Hong Li

1243 related Products with: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

1.00 flask1.00 flask1.00 flask1.00 flask300 units1.00 flask1.00 flask1.00 flask1 SetProtein1.00 flask

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