Rabbit Anti-Human Nestin Antibodies productsSearch results for: Rabbit Anti-Human Nestin Antibodies
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[Preparation and identification of rabbit-anti-human nestin antiserum].
Nestin belongs to the class VI intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396 bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis.Lei Liu, Wan Min Lin, Jun Ji Lin, Xuan Liu, Lan Ping Guo, Ping Xu
