Search results for: Rabbit Anti-KCNJ5 Polyclonal Antibody, Gold conjugated Isotype: IgG
#32199081 
2020/02/24
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Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
Trichinellosis is a serious foodborne zoonotic disease. It is an important threat to public health all over the world. Although anti-Trichinella IgG detection is the most widely used method for diagnosis of trichinellosis, but there is an obvious window between clinical symptoms and positive serology. Gold nanoparticles (AuNPs) can be conjugated with antibodies affording them promising applications for bio-chemical detection. Herein, AuNPs-based ELISA was evaluated for the first time in the detection of Trichinella spiralis circulating antigen (CAg) for its potential as a diagnostic tool of experimental infection. Swiss Albino mice were orally inoculated with 100 muscle larvae/mouse. Animals were sacrificed 6, 8, 10, 12, 14, 16, 22 and 28 day-post infection (dpi). Blood samples were tested for CAg by both standard ELISA and nano-based ELISA using anti-rabbit polyclonal IgG conjugated with AuNPs. CAg was only detected by nano-based ELISA 6, 8, 10 dpi and by both formats 12-28 dpi. Nano-based assay recorded a statistically significant high sensitivity (58.33%, 91.67%) and accuracy (72.22%, 94.44%) 8 and 10 dpi, respectively in comparison to standard ELISA. Both assays showed high sensitivity and accuracy 12-28 dpi. Thus, nano-based ELISA could be considered as an early sensitive diagnostic method for experimental trichinellosis.Maha M Gomaa
1702 related Products with: Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
One 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki500 testsOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki
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#28727765 2017/07/20
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Defining the target and the effect of imatinib on the filarial c-Abl homologue.
Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites.Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman
1786 related Products with: Defining the target and the effect of imatinib on the filarial c-Abl homologue.
min 2 cartons100.00 ul1 ml1200 units1500 Units11mg
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#27940044 2016/12/08
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Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.
HHideyuki Arimitsu, Keiko Sasaki, Takao Tsuji
2253 related Products with: Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.
1 mg1 mg0.2 mg0.2 mg 5 G100tests1 mg2x384 well plate0.1 mg
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#19699744 2009/08/21
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Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.
FJudith Rudolf, Manuela Führer, Brigitte Galler, Parisa Ansari, Christoph Hasenhindl, Sabine Baumgartner
1804 related Products with: Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.
100ul100ug Lyophilized50 ug 1000 1 ml100ug Lyophilized1000 TESTS/0.65ml50 ug 100ug Lyophilized100ug100.00 ul100ug Lyophilized
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#10583677 //
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Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.
Specific nisin polyclonal antibodies (PAb) were produced in rabbits using nisin Z produced by Lactococcus lactis subsp. lactis biovar diacetylactis UL 719. Antisera were obtained from white female New Zealand rabbits that were first immunized with a nisin Z-keyhole limpet haemocyanin conjugate and boosted with free nisin Z. Nisin-specific PAb were purified by affinity chromatography with a yield of 15 mg specific antinisin 100 ml-1 serum. The detection limit of the ELISA test for nisin Z was 0.75 ng ml-1 in buffer but was 1.7 and 3.5 ng ml-1 in milk and complex media broth spiked (5, 10, 20 microg ml-1) with nisin Z, respectively. In nisin Z-spiked samples, the average concentration was between 90 and 107% of actual added amount. In contrast, when the bioassay (microtitration method) was used, only 50-63% of nisin Z biological activity could be detected. In addition, the affinity-purified nisin PAb, antirabbit IgG gold conjugate and transmission electron microscopy were successfully used to locate nisin Z on producing cells and to observe its bactericidal effects against sensitive cells.M Bouksaim, C Lacroix, R Bazin, R E Simard