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Search results for: Rabbit Anti-RNF35 Polyclonal Antibody, FITC conjugated,Isotype: IgG

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#29689714   // To Up

Production and characterization of anti-human IgG F(ab')2 antibody fragment.

In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method. Purity was assessed by SDS-PAGE method. In non-reduced condition only one band was seen in about 150 kDa MW position and in reduced form, two bands were seen in 50 and 25 kDa MW positions. Rabbit IgG was digested by pepsin enzyme. The antibody fragments solution was applied to Gel filtration column to isolate the F(ab')2. Non-reduced SDS-PAGE for determining the purity of F(ab')2 fragment resulted in one band in 100 kDa corresponds to F(ab')2 fragment and a band in 150 kDa MW position corresponds to undigested IgG antibodies. The activities of FITC conjugated F(ab')2 fragment and commercial ones were compared using flowcytometry method. The activity results implied that the FITC conjugated- anti human F(ab')2 fragment worked as efficiently as the commercial one.
Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi

1576 related Products with: Production and characterization of anti-human IgG F(ab')2 antibody fragment.

100ug Lyophilized100ug Lyophilized1 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#27617027   2016/09/09 To Up

Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

Retinal ischemia is a retinal disorder related to retinal vascular occlusion, glaucoma, diabetic retinopathy and age-related macular degeneration. The study aimed to evaluate the protective effects and underlying mechanisms of Chi-Ju-Di-Huang-Wan (CJDHW) against retinal ischemia in rats.
Hsiao-Ming Chao, Lei Hu, Ji-Min Cheng, Xiao-Qian Liu, Jorn-Hon Liu, Wynn Hwai-Tzong Pan, Xiu-Mei Zhang

1144 related Products with: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

100ug100 μg5 100ug50096T2 ml100μg1x96 well plate100ul1mg

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#21620854   2011/05/18 To Up

Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

Hypochlorite is a strong oxidant, generated under pathological conditions, with the potency to introduce chlorine atom into a number of molecules. 3-Chloro- and 3,5-dichlorotyrosine are documented to be generated by this oxidant and their elevated levels were found in many diseases. Thus, we decided to check the possibility of use of FITC-conjugated antibodies for flow cytometric detection of 3-chlorotyrosine residues in human cells (A549, MCF-7, HUVEC-ST) exposed to the action of hypochlorite. Additionally, we compared the effects of chlorohydrins and N-chloroamino acids as chlorine donors. Cell fixation and permeabilization was followed by incubation with rabbit polyclonal anti-3-chlorotyrosine primary antibody and subsequent staining with goat anti-rabbit FITC-labeled secondary antibody. For antibody isotypic control, normal rabbit IgG was employed. Hypochlorite appeared to be the most efficient from the chlorocompounds analyzed in chlorotyrozine generation in all cell lines. Statistically significant increase of fluorescence corresponding to the level of 3-chlorotyrosine residues was found in cells treated with hypochlorite even at non-toxic concentrations (<5μM). This effect was not observed in cells exposed to the action of chlorinated amino acids or chlorohydrins. The use of anti-3-chlorotyrosine antibodies in conjunction with fluorophore-conjugated secondary antibodies analysis allows for detection of 3-chlorotyrosine residues by flow cytometry in cells treated with low doses of hypochlorite.
Agnieszka Robaszkiewicz, Grzegorz Bartosz, Miroslaw Soszynski

1348 related Products with: Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

1 kit100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1.00 flask100ug Lyophilized100ug Lyophilized1.00 flask1.00 flask1 mg1.00 flask

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#20807555   2010/08/31 To Up

Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

In this paper, a sensitive immunoassay method was proposed for Listeria monocytogenes detection by using highly fluorescent bioconjugated nanoparticles probe. (FITC-IgG)-doped fluorescent silica nanoparticles (fsNPs) firstly were synthesized by a microemulsion method and characterized by TEM and fluorescent spectra. Then the prepared fsNPs were conjugated with polyclonal rabbit anti-L. monocytogenes antibody (pAb) and used as indicator probe. A sandwich-type immune affinity reaction between polyclonal rabbit anti-L. monocytogenes antibody coated onto microplate wells, target bacteria and the fsNPs-antibody conjugates subsequently was conducted to detect target L. monocytogenes and assemble the indicator probe onto the wells. The target L. monocytogenes was measured by the fluorescent signals of the assembled indicator probes. Under the optimal conditions, the calibration graph of fluorescent intensity is proportional to the amount of target bacteria over the range of 50-10,320 CFU/mL with a detection limit of 50 CFU/mL. The proposed method has been successfully applied to detect L. monocytogenes in food samples offering the advantages of sensitivity, simplicity, and stability.
Zhouping Wang, Tingting Miu, Huan Xu, Nuo Duan, Xiaoying Ding, Shuang Li

2967 related Products with: Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

96 wells96 tests

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#17723519   2006/03/14 To Up

Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

Listeria monocytogenes can grow at the low temperature commonly used in the storage and transportation of food, and the number of cases of food poisoning caused by L. monocytogenes has increased recently in the US and Europe. Several methods of detecting L. monocytogenes cells have been proposed; however, all existing methods require approximately 48 h incubation. In this study, we attempted rapid detection of L. monocytogenes using flow cytometry (FCM). The method is based on measuring the number of L. monocytogenes cells by using a combination of FCM and immunomagnetic separation (IMS). First, polyclonal antibodies (anti-L. monocytogenes rabbit IgG-FITC) conjugated with fluorescein isothiocyanate (FITC) were reacted with L. monocytogenes cells, and then FCM was applied. The cell numbers were determined by FCM using a traditional colony-counting method in the range of 10(4)-10(8) cells ml(-1). Tetrameric antibody complexes (TAC) were used because they can recognize both magnetic and FITC molecules on the FITC-conjugated antibodies. FITC-labeled L. monocytogenes cells were reacted with a secondary antibody (TAC) bound to magnetic beads. Then, IMS was used. The method is suitable for detection in the range of 10(2)-10(8)cells ml(-1). The FCM assay enumerated the cells within 1 min and the total assay time, including sample preparation, was less than 2 h.
Kyoko Hibi, Akihisa Abe, Eiji Ohashi, Kohji Mitsubayashi, Hideki Ushio, Tetsuhito Hayashi, Huifeng Ren, Hideaki Endo

1435 related Products with: Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

1 kit200 1 kit1 kit1 kit1 kit200 1 kit 5 G1 kit

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#16035233   // To Up

Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

Immunofluorescence detection was performed by tissue sectioning and membrane entrapment of Xylella fastidiosa from the inoculated hybrid selection F8909-08 (Vitis rupestris A. de Serres x V. arizonica/candicans b43-17; resistant) and Chardonnay (susceptible). In both techniques, tissue sections and bacteria-trapped polycarbonate membranes were incubated with specific polyclonal IgG and stained with fluorescein isothiocyanate (FITC)-conjugated IgG from rabbits to X. fastidiosa cells. The stained preparations were observed by fluorescence microscopy. Rapid identification of the bacteria within 3 weeks post inoculation (wpi) was possible in thin cross sections of the petioles, which allowed penetration of the specific antibody. Examination of the bacteria over time was also possible, and allowed observation of bacterial multiplication and invasion of xylem vessels. The membrane entrapment technique was able to isolate bacteria at low concentrations in infected but asymptomatic plants.
Nihal Buzkan, Lászlo Kocsis, M Andrew Walker

2105 related Products with: Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

100ug100 mg1000 tests100ug500 MG100ul25 mg10 mg50 mg50 ug 1000 TESTS/0.65ml25 mg

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#15896799   2005/04/09 To Up

Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

Both monoclonal (e.g. Orthoclone (OKT3), rituximab) and polyclonal (e.g. ATGAM, Thymoglobulin (Thymo)) anti-lymphocyte Abs (ALAs) are used extensively in organ transplantation for immunosuppression induction, desensitization, and treatment of acute rejection. ALAs often interfere with post transplant immunologic monitoring. We describe a method that uses magnetic beads to selectively remove ALAs from patient serum. Rabbit anti-mouse Fc-specific (180 mug), or rabbit anti-mouse Fab-specific (180 microg), or rabbit anti-horse heavy and light chain-specific and rabbit anti-horse F(ab')2 (200 microg) (Jackson Immunoresearch) was adsorbed to 6.7 x 10(8) Dynabeads M-280 conjugated with sheep anti-rabbit IgG (Dynal Biotech). Fifty microliters of normal human serum (NHS) with 2 microg/ml of OKT3 or 100 microg/ml ATGAM, Thymo, or rituximab were incubated with conjugated beads for several incubations. NHS containing ALAs before and after treatment by the protocol were incubated with human lymphocytes and labeled with FITC-antibody to immunoglobulin of the species used to produce the particular ALA. Residual ALA was determined using flow cytometry. Average median channel for serum with or without ALA was 11.1 and 0.120, respectively for OKT3; 64.4 and 0.344 for ATGAM; 108.5 and 0.200 for Thymo; and 1022.5 and 11.4 for rituximab. Treatment lowered the median channel for serum with OKT3 to 0.103, 0.309 for ATGAM, 0.199 for Thymo, and 12.1 for rituximab. ALAs can be effectively removed from serum by the use of magnetic beads conjugated with Ab specific for ALA thereby permitting immunologic monitoring without interference.
Christopher M Bearden, Benita K Book, Richard A Sidner, Mark D Pescovitz

2181 related Products with: Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

100 100UG 100UG0.1 mg 100UG200 100 Tests1 mg100ul 100UG200 1 mg

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#15020090   // To Up

Dual enhancement of triple immunofluorescence using two antibodies from the same species.

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Ayako Nakamura, Toshiki Uchihara

1665 related Products with: Dual enhancement of triple immunofluorescence using two antibodies from the same species.

50 ug Product tipe: Antib50 ug Product tipe: Antib0.1 mg50 ug Product tipe: Antib100 ug Product tipe: Anti100 ul Product tipe: Anti100 μg20 ug Product tipe: Antib100 100ul1 ml

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#12046090   // To Up

Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

To observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs.
Jian-Ping Gong, Li-Li Dai, Chang-An Liu, Chuan-Xin Wu, Yu-Jun Shi, Sheng-Wei Li, Xu-Hong Li

2602 related Products with: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

1.00 flask1.00 flask1.00 flask300 units1.00 flask1.00 flask1.00 flask1.00 flask1 mg1 Set1 Set

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