Search results for: Rabbit Anti-SEN1 Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG
#38156570 
2023/12/26
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Detection of antibodies to the hepatitis E virus in domestic reindeer () in the Republic of Sakha (Yakutia).
Although domestic pigs and wild boars are the main reservoir of zoonotic hepatitis E virus (HEV) genotypes in temperate countries, the presence of antibodies to HEV (anti-HEV) in the indigenous population of circumpolar territories, i.e. outside the habitat of wild and domestic pigs, indicates the presence of an alternative reservoir of the virus. Reindeer () may be a potential reservoir for HEV in the polar regions. The purpose of the study was to determine the prevalence of anti-HEV among domestic reindeer in the Republic of Sakha (Yakutia).V S Kichatova, I A Potemkin, F A Asadi Mobarkhan, T D Rumyantseva, S I Semenov, K K Kyuregyan, M I Mikhailov
1953 related Products with: Detection of antibodies to the hepatitis E virus in domestic reindeer () in the Republic of Sakha (Yakutia).
1 mL100 μg100 μg100 100
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#36270332 2022/10/19
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Protein A-Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G.
Detection and quantification of antibodies, especially immunoglobulin G (IgG), is a cornerstone of ELISAs, many diagnostics, and the development of antibody-based drugs. Current state-of-the-art immunoassay techniques for antibody detection require species-specific secondary antibodies and carefully-controlled bioconjugations. Poor conjugation efficiency degrades assay performance and increases the risk of clinical false positives due to non-specific binding. We developed a generic, highly-sensitive platform for IgG quantification by fusing the IgG-Fc binding Z domain of Staphylococcal Protein A with the ultrabright bioluminescence reporter Nanoluc-luciferase (Nluc). We demonstrated the application of this fusion protein in a sandwich IgG detection immunoassay using surface-bound antigens to capture target IgG and protein A-Nanoluc fusion as the detector. We optimized the platform's sensitivity by incorporating multiple repeats of the Z domain into the fusion protein constructs. Using rabbit and mouse anti-SARS-CoV-2 Nucleoprotein IgGs as model analytes, we performed ELISAs in two different formats, either with SARS-CoV-2 Nucleoprotein as the capture antigen or with polyclonal chicken IgY as the capture antibody. Using standard laboratory equipment, the platform enabled the quantitation of antibody analytes at concentrations as low as 10 pg/mL (67 fM).Suman Nandy, Mary Crum, Katherine Wasden, Ulrich Strych, Atul Goyal, Vijay Maranholkar, William Mo, Binh Vu, Katerina Kourentzi, Richard C Willson
2279 related Products with: Protein A-Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G.
100100ug0.1 mg0.05mg10 mg10000.2 mg50 Test10100 1001000
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#32762697 2020/08/06
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Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach.
Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccinations against FPV among wild felid species have long been practiced in zoos worldwide. However, few studies have assessed the tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests.Chanakan Areewong, Amarin Rittipornlertrak, Boondarika Nambooppha, Itsarapan Fhaikrue, Tawatchai Singhla, Chollada Sodarat, Worapat Prachasilchai, Preeyanat Vongchan, Nattawooti Sthitmatee
1906 related Products with: Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach.
1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)1 kit(96 Wells)
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#32663533 2020/07/11
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A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.
BRamasamy Selvarajan, Prasanya Selvam Kanichelvam, Velusamy Balasubramanian, Sundaram Sethurama Subramanian
1084 related Products with: A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.
96 tests100tests1 kit20 ul100tests25 96 Tests
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#32199081 2020/02/24
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Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
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1275 related Products with: Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
One 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki500 testsOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki
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#32029125 2019/12/10
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Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy.
Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality of the protein starting material is, therefore, an important factor in determining final product composition, purity, and quality. In terms of structural quality, one needs to know both the aggregation content and the tertiary structure of the protein. The measurement of structural quality in solution can thus be difficult, but the use of intrinsic fluorescence measurements might offer a solution because of its high sensitivity, ease of use, and when implemented in via multi-dimensional techniques like polarized Excitation Emission Matrix (pEEM) spectroscopy, its high information content. Here we demonstrate how pEEM measurements can be used as a multi-attribute screening method for protein quality using a polyclonal rabbit immunoglobulin (rIgG) model system. By using both Rayleigh scatter and fluorescence emission in combination with simple chemometric data analysis methods like Principal Component analysis (PCA) and unfolded partial least squares (U-PLS) one can simultaneously measure protein concentration, structural variance, and particle/aggregate composition. Furthermore, one can generate quantitative prediction models for non-reversible aggregation content as described by size exclusion chromatography (SEC) and obtain qualitative information about reversible aggregate content, which cannot be obtained from SEC measurements. In conclusion, the pEEM measurement approach is a potentially useful Process Analytical Technology (PAT) method for downstream processing operations in biopharmaceutical manufacturing.Ana Luiza de Faria E Silva, Saioa Elcoroaristizabal, Alan G Ryder
2862 related Products with: Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy.
96T1 kit1 kit
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#29689714 //
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Production and characterization of anti-human IgG F(ab')2 antibody fragment.
In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method. Purity was assessed by SDS-PAGE method. In non-reduced condition only one band was seen in about 150 kDa MW position and in reduced form, two bands were seen in 50 and 25 kDa MW positions. Rabbit IgG was digested by pepsin enzyme. The antibody fragments solution was applied to Gel filtration column to isolate the F(ab')2. Non-reduced SDS-PAGE for determining the purity of F(ab')2 fragment resulted in one band in 100 kDa corresponds to F(ab')2 fragment and a band in 150 kDa MW position corresponds to undigested IgG antibodies. The activities of FITC conjugated F(ab')2 fragment and commercial ones were compared using flowcytometry method. The activity results implied that the FITC conjugated- anti human F(ab')2 fragment worked as efficiently as the commercial one.Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi
2912 related Products with: Production and characterization of anti-human IgG F(ab')2 antibody fragment.
100ug Lyophilized1 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
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#28727765 2017/07/20
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Defining the target and the effect of imatinib on the filarial c-Abl homologue.
Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites.Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman