Search results for: Rabbit Anti-TFCP2C Polyclonal Antibody
#38633244 
2024/04/03
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Dry and liquid formulations of IBT-V02, a novel multi-component toxoid vaccine, are effective against isolates from low-to-middle income countries.
is the leading cause of skin and soft tissue infections (SSTIs) in the U.S. as well as more serious invasive diseases, including bacteremia, sepsis, endocarditis, surgical site infections, osteomyelitis, and pneumonia. These infections are exacerbated by the emergence of antibiotic-resistant clinical isolates such as methicillin-resistant (MRSA), highlighting the need for alternatives to antibiotics to treat bacterial infections. We have previously developed a multi-component toxoid vaccine (IBT-V02) in a liquid formulation with efficacy against multiple strains of prevalent in the industrialized world. However, liquid vaccine formulations are not compatible with the paucity of cold chain storage infrastructure in many low-to-middle income countries (LMICs). Furthermore, whether our IBT-V02 vaccine formulations are protective against isolates from LMICs is unknown. To overcome these limitations, we developed lyophilized and spray freeze-dried formulations of IBT-V02 vaccine and demonstrated that both formulations had comparable biophysical attributes as the liquid formulation, including similar levels of toxin neutralizing antibodies and protective efficacy against MRSA infections in murine and rabbit models. To enhance the relevancy of our findings, we then performed a multi-dimensional screen of 83 clinical isolates from LMICs (e.g., Democratic Republic of Congo, Palestine, and Cambodia) to rationally down-select strains to test in our models based on broad expression of IBT-V02 targets (i.e., pore-forming toxins and superantigens). IBT-V02 polyclonal antisera effectively neutralized toxins produced by the clinical isolates from LMICs. Notably, the lyophilized IBT-V02 formulation exhibited significant efficacy in various preclinical infection models against the clinical isolates from LMICs, which was comparable to our liquid formulation. Collectively, our findings suggested that lyophilization is an effective alternative to liquid vaccine formulations of our IBT-V02 vaccine against infections, which has important implications for protection from isolates from LMICs.Yu Wang, Ipsita Mukherjee, Arundhathi Venkatasubramaniam, Dustin Dikeman, Nicholas Orlando, Jing Zhang, Roger Ortines, Mark Mednikov, Shardulendra P Sherchand, Tulasikumari Kanipakala, Thao Le, Sanjay Shukla, Mark Ketner, Rajan P Adhikari, Hatice Karauzum, M Javad Aman, Nathan K Archer
1457 related Products with: Dry and liquid formulations of IBT-V02, a novel multi-component toxoid vaccine, are effective against isolates from low-to-middle income countries.
50 ug50 ug20 50 ug1 ml100 mg20 50 ug300 tests50 ug
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#38574703 2024/04/03
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Development and validation of streptavidin-biotin-based double antibody sandwich ELISA for ricin diagnosis.
Ricin is a potential biowarfare agent. It is a phytotoxin isolated from castor seeds. At present there is no antidote available for ricin poisoning, patients only get supportive treatment based on their symptoms. This highlights the importance of early detection to avoid severity of accidents and reduce the risk factor. Considering this, our study aimed to develop a highly sensitive and specific sandwich ELISA for the detection of ricin.Shivani Dixit, Jagrati Parashar, Ram Kumar Dhaked, Abdhesh Kumar, Nandita Saxena
1533 related Products with: Development and validation of streptavidin-biotin-based double antibody sandwich ELISA for ricin diagnosis.
1 kit100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug100ug100ug100ug Lyophilized100ug100ug100ug
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#38537770 2024/03/26
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A novel enzyme-linked ligand-sorbent assay (ELLSA) to screening pulmonary tuberculosis: a retrospective cross-sectional study.
Little knowledge of antigen existence in the pulmonary tuberculosis (PTB) patient serum impeded its development in antigen detection technology, despite its considerable potential.Gang Sheng, Hongqian Chu, Huijuan Duan, Hong Sun, Zhongyao Xie, Zhaogang Sun, Tingming Cao
2384 related Products with: A novel enzyme-linked ligand-sorbent assay (ELLSA) to screening pulmonary tuberculosis: a retrospective cross-sectional study.
100 assays900 tests1 kit(96 Wells)100 assays100 assays1 kit100 assays1 kit(96 Wells)100 assays100 assays
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#38520755 2024/03/12
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Moniezia benedeni infection increases IgE cells in sheep (Ovis aries) small intestine.
The concentration of immunoglobulin (Ig) E is the lowest among serum Igs, but it can induces type I hypersensitivity and plays an important role in anti-parasitic infection. The present study aimed to explore the residence characteristics of IgE cells in the sheep small intestine and the impact of Moniezia benedeni infection on them. The recombinant plasmids pET-28a-IgE were constructed and induced and expressed in Escherichia coli. BL21 (DE3). The rabbit anti-sheep IgE polyclonal antibody was prepared using the obtained recombinant protein as antigen. Finally, the levels of IgE cells in the small intestine of healthy (Control group) and naturally M. benedeni-infected (Infected group) sheep were detected analyzed. The results showed that the rabbit anti-sheep IgE polyclonal antibody with good immunogenicity (titer = 1: 128000) could specifically bind to the heavy chain of natural sheep IgE. In the Control group, the IgE cells were mainly distributed in lamina propria of the small intestine, and the densities were significantly decreased from duodenum to ileum (P<0.05), with respective values of (4.28 cells / 10 μm, 1.80 cells / 10 μm, and 1.44 cells / 10 μm in duodenum, jejunum, and ileum. In the Infected group, IgE cells density were 6.26 cells / 10 μm, 3.01 cells / 10 μm, and 2.09 cells / 10 μm in duodenum, jejunum and ileum respectively, which were significantly higher in all segments compared to the Control group (P<0.05), increasing by 46.26%, 67.22% and 45.14%, respectively. In addition, compared with the Control group, the IgE protein levels were significantly increased in all intestinal segments of the Infected group (P<0.01), however, there was no significant differences among the different intestinal segments within the same group (P>0.05). The results demonstrated that M. benedeni infection could significantly increase the content of IgE and the distribution density of its secreting cells in sheep small intestine. The intestinal mucosal immune system of sheep presented obvious specificity against M. benedeni infection. This lays a good foundation for further exploring molecular mechanisms of the intestinal mucosal immune system monitoring and responding to M. benedeni infection.Jing Pan, Wan-Ling Yao, Li-Ping Liu, Bao-Shan Wang, Wen-Zhu Chai, Zhen Huang, Xi-Ping Fan, Wan-Hong He, Wen-Hui Wang, Wang-Dong Zhang
1103 related Products with: Moniezia benedeni infection increases IgE cells in sheep (Ovis aries) small intestine.
1.00 flask900 tests1.00 flask
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#38421879 2024/02/29
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First-in-human Study With LIS1, a Next-generation Porcine Low Immunogenicity Antilymphocyte Immunoglobulin in Kidney Transplantation.
Polyclonal rabbit antithymocyte globulins (ATGs) are commonly used in organ transplantation as induction. Anti-N-glycolylneuraminic acid carbohydrate antibodies which develop in response to rabbit carbohydrate antigens might lead to unwanted systemic inflammation. LIS1, the first new generation of antilymphocyte globulins (ALGs) derived from double knockout swine, lacking carbohydrate xenoantigens was already tested in nonhuman primates and rodent models.Ondrej Viklicky, Janka Slatinska, Libor Janousek, Juliette Rousse, Pierre-Joseph Royer, Pierre-Louis Toutain, Emanuele Cozzi, Cesare Galli, Gwenaelle Evanno, Odile Duvaux, Jean-Marie Bach, Jean-Paul Soulillou, Magali Giral, Bernard Vanhove, Gilles Blancho
1981 related Products with: First-in-human Study With LIS1, a Next-generation Porcine Low Immunogenicity Antilymphocyte Immunoglobulin in Kidney Transplantation.
100 μg100 μg100 μg100 μg100ug Lyophilized100 μg100 μg100 μg100 μg
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#38420990 2024/02/29
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Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous β-catenin and cytoplasmic β-catenin expression with the outcome of patients with clear cell renal cell carcinoma.
The objective of this study was to investigate the association and combined prognostic significance of the PD-L1, Smoothened protein and β-catenin expressions in patients with clear cell renal cell carcinoma (ccRCC).Aneta Rozsypalova, Blanka Rosova, Alzbeta Filipova, Dimitar Hadzi Nikolov, Renata Chloupkova, Igor Richter, Roman Zachoval, Radoslav Matej, Bohuslav Melichar, Tomas Buchler, Josef Dvorak
1407 related Products with: Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous β-catenin and cytoplasmic β-catenin expression with the outcome of patients with clear cell renal cell carcinoma.
24 reactions 100 U
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#38420701 2024/02/29
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Evaluation of species-specific polyclonal antibodies to detect and differentiate between and .
Neosporosis and toxoplasmosis are major causes of abortion in livestock worldwide, leading to substantial economic losses. Detection tools are fundamental to the diagnosis and management of those diseases. Current immunohistochemistry (IHC) tests, using sera raised against whole parasite lysates, have not been able to distinguish between and We used and recombinant proteins, expressed in and purified using insoluble conditions, to produce specific polyclonal rabbit antisera. We aimed to develop species-specific sera that could be used in IHC on formalin-fixed, paraffin-embedded (FFPE) tissue sections to improve the diagnosis of ruminant abortions caused by protozoa. Two polyclonal rabbit sera, raised against recombinant proteins, anti--rNcSRS2 and anti--rTgSRS2, had specificity for the parasite they were raised against. We tested the specificity for each polyclonal serum using FFPE tissue sections known to be infected with and . The anti--rNcSRS2 serum labeled specifically only infected tissue blocks, and the anti--rTgSRS2 serum was specific to only infected tissues. Moreover, tissues from 52 cattle and 19 sheep previously diagnosed by lesion profiles were tested using IHC with our polyclonal sera and PCR. The overall agreement between IHC and PCR was 90.1% for both polyclonal anti-rNcSRS2 and anti-rTgSRS2 sera. The polyclonal antisera were specific and allowed visual confirmation of protozoan parasites by IHC, but they were not as sensitive as PCR testing.Tanja Lepore, Alastair I Macrae, Germán J Cantón, Carlo Cantile, Henny M Martineau, Javier Palarea-Albaladejo, Stephen Cahalan, Clare Underwood, Frank Katzer, Francesca Chianini
2295 related Products with: Evaluation of species-specific polyclonal antibodies to detect and differentiate between and .
50 ug 50 ug 50 ug 0.1 mg96T1 ml0.1 ml200ul1000 TESTS/0.65ml50 ug 50 ug 100 ug
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#38385694 2024/02/22
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Discovery of Two Novel Immunoepitopes and Development of Peptide-based Sarcoidosis Immunoassay.
Sarcoidosis is a systemic granulomatous disorder associated with hypergammaglobulinemia and the presence of autoantibodies. The specific antigens initiating granulomatous inflammation in sarcoidosis are unknown and there is no specific test available to diagnose sarcoidosis. To discover novel sarcoidosis antigens, we developed a high-throughput T7 phage display library derived from the sarcoidosis cDNA and identified numerous clones differentiating sarcoidosis from other respiratory diseases. After clone sequencing and homology search, we identified two epitopes (Cofilinμ and Chain A) that specifically bind to serum IgGs of sarcoidosis patients.Changya Peng, Jaya Talreja, Brennen Steinbauer, Kazuhiko Shinki, Laura L Koth, Lobelia Samavati