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Search results for: Rabbit Anti-TSPAN14 Polyclonal Antibody, FITC conjugated,Isotype: IgG

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#29689714   // To Up

Production and characterization of anti-human IgG F(ab')2 antibody fragment.

In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method. Purity was assessed by SDS-PAGE method. In non-reduced condition only one band was seen in about 150 kDa MW position and in reduced form, two bands were seen in 50 and 25 kDa MW positions. Rabbit IgG was digested by pepsin enzyme. The antibody fragments solution was applied to Gel filtration column to isolate the F(ab')2. Non-reduced SDS-PAGE for determining the purity of F(ab')2 fragment resulted in one band in 100 kDa corresponds to F(ab')2 fragment and a band in 150 kDa MW position corresponds to undigested IgG antibodies. The activities of FITC conjugated F(ab')2 fragment and commercial ones were compared using flowcytometry method. The activity results implied that the FITC conjugated- anti human F(ab')2 fragment worked as efficiently as the commercial one.
Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi

2983 related Products with: Production and characterization of anti-human IgG F(ab')2 antibody fragment.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#27617027   2016/09/09 To Up

Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

Retinal ischemia is a retinal disorder related to retinal vascular occlusion, glaucoma, diabetic retinopathy and age-related macular degeneration. The study aimed to evaluate the protective effects and underlying mechanisms of Chi-Ju-Di-Huang-Wan (CJDHW) against retinal ischemia in rats.
Hsiao-Ming Chao, Lei Hu, Ji-Min Cheng, Xiao-Qian Liu, Jorn-Hon Liu, Wynn Hwai-Tzong Pan, Xiu-Mei Zhang

2417 related Products with: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.

100ug100 μg96T100ug5 2 ml500100μg1x96 well plate100ul100ug

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#21620854   2011/05/18 To Up

Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

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Agnieszka Robaszkiewicz, Grzegorz Bartosz, Miroslaw Soszynski

1252 related Products with: Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.

1 kit100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1.00 flask100ug Lyophilized100ug Lyophilized1.00 flask1.00 flask1 mg1.00 flask

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#20807555   2010/08/31 To Up

Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

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Zhouping Wang, Tingting Miu, Huan Xu, Nuo Duan, Xiaoying Ding, Shuang Li

1190 related Products with: Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.

96 wells

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#17723519   2006/03/14 To Up

Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

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Kyoko Hibi, Akihisa Abe, Eiji Ohashi, Kohji Mitsubayashi, Hideki Ushio, Tetsuhito Hayashi, Huifeng Ren, Hideaki Endo

2939 related Products with: Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.

1 kit1 kit200 1 kit1 kit1 kit200 1 kit 5 G1 kit

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#16035233   // To Up

Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

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Nihal Buzkan, Lászlo Kocsis, M Andrew Walker

2336 related Products with: Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.

100 mg1000 tests100ug100ug100ul25 mg10 mg500 MG50 ug 25 mg1000 TESTS/0.65ml96T

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#15896799   2005/04/09 To Up

Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

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Christopher M Bearden, Benita K Book, Richard A Sidner, Mark D Pescovitz

1578 related Products with: Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.

100 100UG 100UG0.1 mg 100UG200 100 Tests1 mg1 mg 100UG1 mg200

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#15020090   // To Up

Dual enhancement of triple immunofluorescence using two antibodies from the same species.

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Ayako Nakamura, Toshiki Uchihara

1462 related Products with: Dual enhancement of triple immunofluorescence using two antibodies from the same species.

50 ug Product tipe: Antib0.1 mg50 ug Product tipe: Antib100 ug Product tipe: Anti50 ug Product tipe: Antib100 ul Product tipe: Anti100 μg20 ug Product tipe: Antib100 1 ml 100ul

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#12730261   2003/04/28 To Up

In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.

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M M Maryani, M V Morse, G Bradley, H R Irving, D M Cahill, C A Gehring

2974 related Products with: In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.

4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo100ug

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#12046090   // To Up

Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

To observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs.
Jian-Ping Gong, Li-Li Dai, Chang-An Liu, Chuan-Xin Wu, Yu-Jun Shi, Sheng-Wei Li, Xu-Hong Li

2160 related Products with: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

1.00 flask1.00 flask1.00 flask1.00 flask300 units1.00 flask1.00 flask1.00 flask1 SetProtein1.00 flask

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