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Purification of the murine heat-stable antigen from erythrocytes.

The rat anti-mouse erythrocyte (MRBC) monoclonal antibody (mAb), R13, has been developed. The MRBC membrane protein recognized by R13 (R13-Ag) can be purified by loading the butanol-extracted MRBC membrane solution on a R13-conjugated Cellulofine column in the presence of 0.1% CHAPS followed by elution with 1% CHAPS. The amino acid sequence of the affinity-purified R13-Ag corresponded to that predicted from the cDNA for the murine heat-stable antigen. It was revealed that the actual heat-stable antigen was composed of 27 amino acids.
Y Hitsumoto, A Nakano, H Ohnishi, F Hamada, S Saheki, N Takeuchi

2276 related Products with: Purification of the murine heat-stable antigen from erythrocytes.

96 tests96 tests96T100 U100 Tests / Kit50ul (1mg/ml)50 purifications500 ml100 µg25 μg25ml 0.1ml (1mg/ml)

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