Search results for: Rat Red Blood Cells 25ml
#20883155 
//
To Up
The protective role of Tropaeolum majus on blood and liver toxicity induced by diethyl maleate in rats.
The protective role of Tropaelum majus (T.majus) methyl alcohol extract and vitamin E in the case of toxic effect induced by diethyl maleate was evaluated. Forty-two male albino rats were divided into seven groups of six rats each for 15 days. Group 1: normal control group. Group 2: taken daily oral dose of paraffin oil (0.25ml/100g b.wt rat). Group 3: received daily oral dose of vitamin E (100mg/kg b.wt rat). Group 4: taken daily oral dose of 10% of the LD50 of T.majus methyl alcohol extract. Groups 5–7: injected intra-peritoneally with diethyl maleate (5 μl/100g b.wt rat) but groups 6 and 7 received a daily oral dose of either vitamin E or 10% of the LD50 of T.majus methyl alcohol extract 1h prior to diethyl maleate injection. The present results revealed that diethyl maleate induced serum aspartate and alanine aminotransferases enzymes activities decreased in serum, but their activities in the hepatic tissue showed an increase. Glutathione and glucose-6-phosphate dehydrogenase levels showed a decrease, but thiobarbituric acid reactive substances level showed an increase in both serum and liver tissue. Serum and liver proteins decreased in serum and liver tissue. A significant decrease in blood parameters (hemoglobin, hematocrit, as well as red and white blood cells) and serum glucose occurred. Histopathological results showed that diethyl maleate induced a hoop of edema in the hepatic periportal area; while T.majus methyl alcohol extract or vitamin E prior to diethyl maleate injection shift blood and liver toxicity induced by diethyl maleate towards normal values and preserved hepatic lobular architecture. In conclusion, pre-treatment with either T.majus methyl alcohol extract or vitamin E provide protection against blood and liver toxicity induced by diethyl maleate in rats, these results were confirmed by histological examinations.Khaled M M Koriem, Mahmoud S Arbid, Nadia F I El-Gendy
1325 related Products with: The protective role of Tropaeolum majus on blood and liver toxicity induced by diethyl maleate in rats.
100 UG900 tests
Related Pathways
#6828455 //
To Up
Chromatography of erythroblasts on immobilized transferrin.
Affinity chromatography with immobilized transferrin has been used to effect a partial purification of erythroblasts and reticulocytes from bone marrow. Human transferrin was covalently attached to Sepharose CL-2B, Bio-Gel A-1.5, A-0.5, P-20, P-6, P-4, and P-2, and Sephadex G-75. Nucleated erythroid cells bound to the greatest degree to the polyacrylamide P-6 bead, whereas reticulocytes had the greatest affinity for the Sepharose CL-2B bead. The polyacrylamide P-6 bead has a greater density of surface groups than the Sepharose bead, and this correlates to the greater density of transferrin receptors on erythroblasts. The beads were used in a column which contained a central stirring bar with multiple crosspieces. The stirrer provided a method to uniformly agitate the beads to facilitate elution of the cells bound to the matrix. Approximately 1-2 x 10(8) bone marrow cells could be applied to a 25-ml matrix bed. This procedure resulted in a considerable resolution of cell types and gave a high yield of reticulocytes and erythroblasts from rat and rabbit bone marrow.D A Juckett, D E Hultquist