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#34118646   2021/06/09 To Up

The immunomodulatory effects of topiramate on azoxymethane-induced colon carcinogenesis in rats: The role of the inflammatory cascade, vascular endothelial growth factor, AKT/mTOR/MAP kinase signaling and the apoptotic markers.

Colon cancer is a malignant condition that affects the lower gastrointestinal tract and has unfavorable prognosis. Its mechanisms range from enhanced production of reactive oxygen species, inflammatory changes in the colon microenvironment and affection of the apoptotic pathways. Due to the high incidence of resistance of colon cancer to the traditional chemotherapeutic agents, a need for finding safe/effective agents that can attenuate the malignant changes had emerged.
Ahmed M Kabel, Ahmed M Ashour, Dina A Ali, Hany H Arab

1097 related Products with: The immunomodulatory effects of topiramate on azoxymethane-induced colon carcinogenesis in rats: The role of the inflammatory cascade, vascular endothelial growth factor, AKT/mTOR/MAP kinase signaling and the apoptotic markers.

1100.00 ul50 IU

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#34118644   2021/06/09 To Up

Expression of angiogenesis-related proteins in bone marrow mesenchymal stem cells induced by osteoprotegerin during osteogenic differentiation in rats.

This study aimed to discuss the expression of angiogenesis-related proteins in bone marrow mesenchymal stem cells (BMSCs) induced by osteoprotegerin (OGP) during osteogenic differentiation in rats, and to analyze the effect of fracture healing inflammatory factor TNF-ɑ on the osteogenic differentiation of BMSCs of rats. BMSCs isolated and cultured from the third generation rats were taken as the research object. According to the addition amount of OGP, BMSCs were divided into control group, OGP (10 mol/L) group, OGP (10 mol/L) group, and OGP (10 mol/L) group. The cell growth and morphological characteristics of each group were observed by inverted phase contrast microscope, the cell proliferation rate was measured by MTT method, angiogenesis-related markers (platelet growth factor (VEGF), cingulate protein 5 (Fbln5), and angiogenin-like protein 4 (Angptl4)) were quantitatively detected by Western blot, and the effect of TNF-ɑ on osteogenic differentiation was detected by CCK. Compared with the control group, MTT results showed that the value-added rate of cells in the OGP (10 mol/L) group reached the maximum at 9 days (P < 0.05). The ALP activity in osteoblasts in the OGP (10 mol/L) group reached the maximum at 9 days (P < 0.01). The OGP (10 mol/L) group had the highest expression of vascular regeneration proteins (VEGF, Fbln5, and Angptl4) (P < 0.05). CCK analysis showed that the TNF-ɑ (1.0 ng/mL) group showed a significant increase in absorbance compared with the control group on 6 days (P < 0.05), and the OD value of the TNF-ɑ (10 ng/mL) group decreased at all time points (P < 0.05). Overall, 10 mol/L OGP can induce the proliferation and osteogenic differentiation of MSCs, and promote the expression of angiogenesis-related proteins (VEGF, Fbln5, and Angptl4) during osteogenic differentiation. Besides, 1.0 ng/mL of TNF-ɑ can also promote osteogenesis differentiation of BMSCs in the short term.
Wei Wang, Bo Wang

2586 related Products with: Expression of angiogenesis-related proteins in bone marrow mesenchymal stem cells induced by osteoprotegerin during osteogenic differentiation in rats.

24 wells2 Pieces/Box1 mg2 Pieces/Box10 ug1 mg501010

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#34118565   2021/06/05 To Up

Metabolic maturation of differentiating cardiosphere-derived cells.

Cardiosphere-derived cells (CDCs) can be expanded in vitro and induced to differentiate along the cardiac lineage. To recapitulate the phenotype of an adult cardiomyocyte, differentiating progenitors need to upregulate mitochondrial glucose and fatty acid oxidation. Here we cultured and differentiated CDCs using protocols aimed to maintain stemness or to promote differentiation, including triggering fatty acid oxidation using an agonist of peroxisome proliferator-activated receptor alpha (PPARα). Metabolic changes were characterised in undifferentiated CDCs and during differentiation towards a cardiac phenotype. CDCs from rat atria were expanded on fibronectin or collagen IV via cardiosphere formation. Differentiation was assessed using flow cytometry and qPCR and substrate metabolism was quantified using radiolabelled substrates. Collagen IV promoted proliferation of CDCs whereas fibronectin primed cells for differentiation towards a cardiac phenotype. In both populations, treatment with 5-Azacytidine induced a switch towards oxidative metabolism, as shown by changes in gene expression, decreased glycolytic flux and increased oxidation of glucose and palmitate. Addition of a PPARα agonist during differentiation increased both glucose and fatty acid oxidation and expression of cardiac genes. We conclude that oxidative metabolism and cell differentiation act in partnership with increases in one driving an increase in the other.
Khadijeh Kathy Pakzad, Jun Jie Tan, Stephanie Anderson, Mary Board, Kieran Clarke, Carolyn A Carr

2807 related Products with: Metabolic maturation of differentiating cardiosphere-derived cells.

4 x 96-well plate1.00 flask215ml 500 ml 2 mL1.00 flask100ul2ug125ml

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#34118433   2021/06/09 To Up

Effect of zinc depletion/repletion on intestinal iron absorption and iron status in Rats.

Iron and zinc deficiencies likely coexist in general population. We have previously demonstrated that zinc treatment induces while zinc deficiency inhibits iron absorption in intestinal cell culture models, but this needs to be tested in vivo. In the present study we assessed intestinal iron absorption, iron status (haemoglobin (Hb), red blood cell (RBC) number, plasma ferritin, transferrin receptor (TfR), hepcidin) and tissue iron levels in zinc depleted, replete and pair fed control rats. Zinc depletion led to reduction in body weight, tissue zinc levels, intestinal iron absorption, protein and mRNA expression of iron transporters, the divalent metal ion transporter-1, hephaestin and ferroportin, but elevated the intestinal and liver tissue iron levels compared with the pair fed control rats. Zinc repletion led to a significant weight gain compared to zinc deficient rats and normalized the iron absorption, iron transporter expression, tissue iron levels to that of pair fed control rats. Surprisingly, Hb levels and RBC number reduced significantly in zinc repleted rats, which could be due to rapid weight gain. Together, these results indicate that whole body zinc status has profound influence on growth, intestinal absorption and systemic utilization of iron, mediated via modulation of iron transporter expression.
Palsa Kondaiah, Ravindranadh Palika, Purnachandra Mashurabad, Puneeta Singh Yaduvanshi, Paul A Sharp, Raghu Pullakhandam

2501 related Products with: Effect of zinc depletion/repletion on intestinal iron absorption and iron status in Rats.

100ug 1 kit(s) 0.1 mg 100ul100/kit 250 ml 20 96tests96 wells100ug Lyophilized 1KG100ul

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#34118415   2021/06/09 To Up

Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in rat Degenerative change in monosodium iodoacetate (MIA)-injected intervertebral disc.

Low back pain is one of the most common musculoskeletal disorders. While the pathology of intervertebral disc (IVD) degeneration has been modeled using various biological methods, these models are inadequate for simulating similar pathological states in humans.
Hye Rim Suh, Hwi-Young Cho, Hee Chul Han

1321 related Products with: Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in rat Degenerative change in monosodium iodoacetate (MIA)-injected intervertebral disc.

0.1ml (1mg/ml)0.1ml 100ul50ul100ug Lyophilized100ug Lyophilized100ug Lyophilized1mg0.1ml0.1ml (1mg/ml)

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#34118364   2021/06/09 To Up

HAART exacerbates testicular damage and impaired spermatogenesis in anti-Koch-treated rats via dysregulation of lactate transport and glutathione content.

Highly active anti-retroviral therapy (HAART) is an effective anti-retroviral cocktail. Similarly, anti-Koch is highly potent against Mycobacterium tuberculosis. However, these drugs have been shown to impair male fertility. This study investigated the impact of HAART and anti-Koch, when used alone and co-administered, on testicular and sperm integrity. Thirty-two adult male Wistar rats were assigned randomly into four groups (n = 8), namely normal control, HAART-treated, anti-Koch-treated, and HAART + anti-Koch-treated. The doses of drugs were the human equivalent doses for rats. Administration was once daily per os and lasted for eight weeks. HAART aggravated anti-Koch-induced reduction in testicular and penile weights. In addition, anti-Koch also led to a distortion of testicular cytoarchitecture, disturbed spermatogenesis, and caused low sperm quality, including sperm dysmotility. More so, anti-Koch led to a significant elevation of uric acid and dysregulation of testicular lactate transport and glutathione content. These events were accompanied by enhanced lipid peroxidation and inflammation of the testicular tissue and reduced testicular and sperm DNA integrity. These adverse effects of anti-Koch were aggravated by co-administration of HAART. Thus, our results infer that HAART exacerbates anti-Koch-induced impairment of spermatogenesis and testicular and sperm toxicity through up-regulation of uric acid generation and dysregulation of lactate transport and glutathione system.
R E Akhigbe, M A Hamed, A O Aremu

2769 related Products with: HAART exacerbates testicular damage and impaired spermatogenesis in anti-Koch-treated rats via dysregulation of lactate transport and glutathione content.

100 μg1000 TESTS/0.65ml100ul100 μg100.00 ul100ul0.1 mg200 1 ml1000 100 μg

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#34118343   2021/06/09 To Up

Leojaponin inhibits NLRP3 inflammasome activation through restoration of autophagy via upregulating RAPTOR phosphorylation.

Duan Teng Yimu decoction is a Chinese herbal medicine compound with proven therapeutic effects on inflammasome-related diseases, such as rheumatoid arthritis. This decoction consists of three Chinese herbal medicines, including Leonurus japonicus (L. japonicus), which promotes the blood circulation and exhibits detumescence activity, traditionally curing gynecologic and inflammasome diseases.
Xing-Jie Zhang, Kun Shang, Yu-Kun Pu, Qi Wang, Ting-Ting Wang, Yan Zou, Yong-Mei Wang, Yao-Jun Xu, Xiao-Li Li, Rui-Han Zhang, Wei-Lie Xiao

1042 related Products with: Leojaponin inhibits NLRP3 inflammasome activation through restoration of autophagy via upregulating RAPTOR phosphorylation.

8 Sample Kit1 Set100 ug/vial2 mg1 Set1 Set1 Set1 Set20μg/vial1 Set1 Set100ug/vial

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#34118312   2021/06/09 To Up

High concentrations of ammonia induced cytotoxicity and bronchoconstriction in a precision-cut lung slices rat model.

Exposure to high concentrations of ammonia (NH) can cause life-threatening lung damages. The objective of this study was to establish a translational in vitro model for NH-induced lung injury. Precision-cut lung slices (PCLS) from rats were exposed to NH and toxicological responses and cell viability were quantified by analysis of LDH, WST-1, inflammatory mediators (IL-1β, IL-6, CINC-1, MMP-9, RAGE and IL-18), and by microscopic evaluation of bronchoconstriction induced by electric-field-stimulation (EFS) or methacholine (MCh). Different treatment strategies were assessed to prevent or reverse the damages caused by NH using anti-inflammatory, anti-oxidant or neurologically active drugs. Exposure to NH caused a concentration-dependent increase in cytotoxicity (LDH/WST-1) and IL-1β release in PCLS medium. None of the treatments reduced cytotoxicity. Deposition of NH (24-59 mM) on untreated PCLS elicited an immediate concentration-dependent bronchoconstriction. Unlike MCh, the EFS method did not constrict the airways in PCLS at 5 h after NH-exposure (47-59 mM). Atropine and TRP-channel antagonists blocked EFS-induced bronchoconstriction but these inhibitors could not block the immediate NH-induced bronchoconstriction. In conclusion, NH exposure caused cytotoxic effects and lung damages in a concentration-dependent manner and this PCLS method offers a way to identify and test new concepts of medical treatments and biomarkers that may be of prognostic value.
Lina Ågren, Linda Elfsmark, Christine Akfur, Sofia Jonasson

2140 related Products with: High concentrations of ammonia induced cytotoxicity and bronchoconstriction in a precision-cut lung slices rat model.

4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box

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