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Search results for: Recombinant

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#34563097   2021/09/25 To Up

Effectiveness of a synthetic human recombinant epidermal growth factor in diabetic patients wound healing: Pilot, double-blind, randomized clinical controlled trial.

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Bianca Campos Oliveira, Beatriz Guitton Renaud Baptista de Oliveira, Gabriela Deutsch, Fernanda Soares Pessanha, Selma Rodrigues de Castilho

2167 related Products with: Effectiveness of a synthetic human recombinant epidermal growth factor in diabetic patients wound healing: Pilot, double-blind, randomized clinical controlled trial.

5 x 50 ug5 x 50 ug100 μg50 ug50 ug50 ug20 ug4 Membranes/Box5 x 50 ug100.00 ug8 Sample Kit

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#34562586   2021/09/22 To Up

Soluble cytoplasmic expression of bioactive recombinant porcine-human chimeric uricase mutant employing MBP-SUMO fusion system.

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Zhenlong Zhou, Hui Zhao, Ligang Zhang, Qiuling Xie, Qiwei Liu, Mingjie Tong, Xiangwei Yu, Sheng Xiong

1803 related Products with: Soluble cytoplasmic expression of bioactive recombinant porcine-human chimeric uricase mutant employing MBP-SUMO fusion system.

20.1mg10 2x 100ug2 10525 50210 50 ug

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#34562449   2021/09/22 To Up

The predicted stem-loop structure in the 3'-end of the human norovirus antigenomic sequence is required for its genomic RNA synthesis by its RdRp.

The norovirus genome consists of a single positive-stranded RNA. The mechanism by which this single-stranded RNA genome is replicated is not well understood. To reveal the mechanism underlying the initiation of the norovirus genomic RNA synthesis by its RNA-dependent RNA polymerase (RdRp), we used an in vitro assay to detect the complementary RNA synthesis activity. Results showed that the purified recombinant RdRp was able to synthesize the complementary positive-sense RNA from a 100-nt template corresponding to the 3'-end of the viral antisense genome sequence, but that the RdRp could not synthesize the antisense genomic RNA from the template corresponding to the 5'-end of the positive-sense genome sequence. We also predicted that the 31 nt region at the 3'-end of the RNA antisense template forms a stem-loop structure. Deletion of this sequence resulted in the loss of complementary RNA synthesis by the RdRp, and connection of the 31 nt to the 3'-end of the inactive positive-sense RNA template resulted in the gain of complementary RNA synthesis by the RdRp. Similarly, an electrophoretic mobility shift assay further revealed that the RdRp bound to the antisense RNA specifically, but was dependent on the 31 nt at the 3'-end. Therefore, based on this observation and further deletion and mutation analyses, we concluded that the predicted stem-loop structure in the 31 nt end and the region close to the antisense viral genomic stem sequences are both important for initiating the positive-sense human norovirus genomic RNA synthesis by its RdRp.
Takashi Shimoike, Tsuyoshi Hayashi, Tomoichiro Oka, Masamichi Muramatsu

2029 related Products with: The predicted stem-loop structure in the 3'-end of the human norovirus antigenomic sequence is required for its genomic RNA synthesis by its RdRp.

11mg102 100ul

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#34562154   2021/09/25 To Up

Wnt3a and ASCs are capable of restoring mineralization in staph aureus-infected primary murine osteoblasts.

Bone infections are one of the main reasons for impaired bone regeneration and non-union formation. In previous experimental animal studies we could already demonstrate that bone defects due to prior infections showed a markedly reduced healing capacity, which could effectively be enhanced via application of Wnt3a and Adipose-derived stromal cells (ASCs). For a more in-depth analysis, we investigated proliferation and mineralization of cultured osteoblasts infected with staph aureus and sought to investigate effects of Wnt3a and ASCs on infected osteoblasts.
Johannes Maximilian Wagner, Yonca Steubing, Mehran Dadras, Christoph Wallner, Sebastian Lotzien, Julika Huber, Alexander Sogorski, Maxi Sacher, Felix Reinkemeier, Stephanie Dittfeld, Mustafa Becerikli, Marcus Lehnhardt, Björn Behr

2763 related Products with: Wnt3a and ASCs are capable of restoring mineralization in staph aureus-infected primary murine osteoblasts.

0.2 mg1 L.0.2 mg0.5 mg100 µg200 250.2 mg100 µg200 100 μg1 mg

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#34561988   2021/09/24 To Up

Is Fibromyalgia Associated with Borrelia Specific T Lymphocytes?

Although fibromyalgia is a common cause of chronic musculoskeletal pain, its aetiology and pathophysiology are uncertain. It has recently been suggested that fibromyalgia symptomatology represents a T lymphocyte-mediated immune response to pathogens which are known risk factors for autoimmune diseases. One major suggested candidate pathogen is the bacterial genus Borrelia. However, to date this hypothesis has not been tested.
Basant K Puri, Gary S Lee, Armin Schwarzbach

2083 related Products with: Is Fibromyalgia Associated with Borrelia Specific T Lymphocytes?

25 µg100 ml1 ml0.25 mg0.1 ml25 µg0.2 mg0.1 mg100 TESTS0.2 mg0.1 mg250 ml

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#34561441   2021/09/24 To Up

Directed evolution of rRNA improves translation kinetics and recombinant protein yield.

In bacteria, ribosome kinetics are considered rate-limiting for protein synthesis and cell growth. Enhanced ribosome kinetics may augment bacterial growth and biomanufacturing through improvements to overall protein yield, but whether this can be achieved by ribosome-specific modifications remains unknown. Here, we evolve 16S ribosomal RNAs (rRNAs) from Escherichia coli, Pseudomonas aeruginosa, and Vibrio cholerae towards enhanced protein synthesis rates. We find that rRNA sequence origin significantly impacted evolutionary trajectory and generated rRNA mutants with augmented protein synthesis rates in both natural and engineered contexts, including the incorporation of noncanonical amino acids. Moreover, discovered consensus mutations can be ported onto phylogenetically divergent rRNAs, imparting improved translational activities. Finally, we show that increased translation rates in vivo coincide with only moderately reduced translational fidelity, but do not enhance bacterial population growth. Together, these findings provide a versatile platform for development of unnatural ribosomal functions in vivo.
Fan Liu, Siniša Bratulić, Alan Costello, Teemu P Miettinen, Ahmed H Badran

2694 related Products with: Directed evolution of rRNA improves translation kinetics and recombinant protein yield.

100ul100102021mg20101mg2100 50.00 ug

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#34561300   // To Up

Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the current COVID-19 pandemic, is one of the biggest threats to public health. However, the dynamic of SARS-CoV-2 infection remains poorly understood. Replication-competent recombinant viruses expressing reporter genes provide valuable tools to investigate viral infection. Low levels of reporter gene expressed from previous reporter-expressing recombinant (r)SARS-CoV-2 in the locus of the open reading frame (ORF)7a protein have jeopardized their use to monitor the dynamic of SARS-CoV-2 infection in vitro or in vivo. Here, we report an alternative strategy where reporter genes were placed upstream of the highly expressed viral nucleocapsid (N) gene followed by a porcine tescherovirus (PTV-1) 2A proteolytic cleavage site. The higher levels of reporter expression using this strategy resulted in efficient visualization of rSARS-CoV-2 in infected cultured cells and excised lungs or whole organism of infected K18 human angiotensin converting enzyme 2 (hACE2) transgenic mice. Importantly, real-time viral infection was readily tracked using a noninvasive in vivo imaging system and allowed us to rapidly identify antibodies which are able to neutralize SARS-CoV-2 infection in vivo. Notably, these reporter-expressing rSARS-CoV-2, in which a viral gene was not deleted, not only retained wild-type (WT) virus-like pathogenicity in vivo but also exhibited high stability in vitro and in vivo, supporting their use to investigate viral infection, dissemination, pathogenesis, and therapeutic interventions for the treatment of SARS-CoV-2 in vivo.
Chengjin Ye, Kevin Chiem, Jun-Gyu Park, Jesus A Silvas, Desarey Morales Vasquez, Julien Sourimant, Michelle J Lin, Alexander L Greninger, Richard K Plemper, Jordi B Torrelles, James J Kobie, Mark R Walter, Juan Carlos de la Torre, Luis Martinez-Sobrido

2133 related Products with: Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses.

10mg5 mg1mg~2x10(6) cells1096 tests100ug Lyophilized50 mg

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#34561142   2021/09/21 To Up

A non-inferiority trial comparing two recombinant vaccines (Hepa-B vs. Engerix-B) for hepatitis B among adults in Dhaka, Bangladesh.

Worldwide Hepatitis B is known as one of the imperative causes of mortality and morbidity as well as occupational health hazard among health workers. Bangladesh is intermediate endemic country for Hepatitis B infection for which the government has introduced hepatitis B vaccination into the Expanded Programme on Immunization (EPI) nationwide since 2009 for new born children. However, the people who were born before 2009, was dependent on imported hepatitis B vaccine as there was no locally manufactured hepatitis B vaccine in Bangladesh. Hence, we conducted a randomized observer blinded non-inferiority clinical trial to assess the immunogenicity and safety of the locally manufactured Hepa-B vaccine in comparison with World Health Organization prequalified Engerix-B vaccine. Total 158 eligible adult participants were enrolled in this study with mean age of 30 and 29 years old in Hepa-B and Engerix-B groups, respectively. Both the vaccines were administered intramuscularly at 0, 1 and 6 months schedule. Baseline and post vaccination anti-HBs titers were measure at different time points. Seroconversion rate post three doses of Hepa-B vaccine was 98.67% similar to the comparator Engerix-B vaccine which was 100%. The geometric mean test ratios of both vaccines at all analysis time points were found > 0.5 predefined non-inferiority margin. Soreness at the injection site was the most common symptom for both the vaccines which resolved without any complication. No serious adverse event was reported throughout the study period. These results suggest that locally manufactured hepatitis B vaccine 'Hepa-B' vaccine is non-inferior to the well-known licensed 'Engerix-B' vaccine. ClinicalTrials.gov NCT03627507.
Fahima Chowdhury, Afroza Akter, Taufiqur Rahman Bhuiyan, Imam Tauheed, Tasnuva Ahmed, Faisal Ahmmed, Faez Ahmed, Mahbubul Karim, Mohammad Mainul Ahasan, Masudur Rahman Mia, Mir Mohammad Ibna Masud, Abdul Wahab Khan, Muhammad Masum Billah, Zebun Nahar, Imran Khan, Md Rahad Hossain, A Z M Ariful Islam, Alex S Panday, Md Muktadir Rahman Ashik, Firdausi Qadri

1049 related Products with: A non-inferiority trial comparing two recombinant vaccines (Hepa-B vs. Engerix-B) for hepatitis B among adults in Dhaka, Bangladesh.

10ìg100 μg100 μg

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#34560990   2021/08/21 To Up

Bioactive chitosan biguanidine-based injectable hydrogels as a novel BMP-2 and VEGF carrier for osteogenesis of dental pulp stem cells.

Nowadays, vascularization and mineralization of bone defects is the main bottleneck in the bone regeneration field that is needed to be overcome and developed. Here, we prepared novel in-situ formed injectable hydrogels based on chitosan biguanidine and carboxymethylcellulose loaded with vascular endothelial growth factor (VEGF) and recombinant Bone morphogenetic protein 2 (BMP-2) and studied its influence on osteoblastic differentiation of dental pulp stem cells (DPSCs). The sequential release behavior of the VEGF and BMP-2 from hydrogels adjusted with the pattern of normal human bone growth. MTT assay exhibited that these hydrogels were non-toxic and significantly increased DPSCs proliferation. The Real-time PCR and Western blot analysis on CG11/BMP2-VEGF showed significantly higher gene and protein expression of ALP, COL1α1, and OCN. These results were confirmed by mineralization assay by Alizarin Red staining and Alkaline phosphatase enzyme activity. Based on these evaluations, these hydrogel holds potential as an injectable bone tissue engineering platform.
Baharak Divband, Marziyeh Aghazadeh, Zahraa Haleem Al-Qaim, Mohammad Samiei, Falah H Hussein, Alireza Shaabani, Shahriar Shahi, Roya Sedghi

2480 related Products with: Bioactive chitosan biguanidine-based injectable hydrogels as a novel BMP-2 and VEGF carrier for osteogenesis of dental pulp stem cells.

25 G200ul200ul100 µg50 µg100Tests2500 assays

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#34560901   2021/09/25 To Up

Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells.

Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to produce recombinant proteins in rice suspension cells. The OsMYBS2 transcription factor was shown recently to reduce activation of the αAmy3 promoter by competing for the binding site of the TA box of the αAmy3 promoter with the potent OsMYBS1 activator. In this study, rice suspension cells were genetically engineered to silence OsMYBS2 to enhance the production of recombinant proteins.
Desyanti Saulina Sinaga, Shin-Lon Ho, Chung-An Lu, Su-May Yu, Li-Fen Huang

1618 related Products with: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells.

1002 Pieces/Box100 units50 101mg1 mg1mg100 21mg1 mg

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