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#34563048   2021/09/08 To Up

Exhibits Anti-Oncogenic Effect in Hepatocellular Carcinoma, HepG2 Cancer Cell Line by Bcl-2 Mediated Apoptotic Pathway and Mitochondrial Cytochrome C Release.

: is an important species of the Asteraceae family with several purposes in traditional medicine. This study intended to explore the cytotoxic effect of on HepG2 cancer cell proliferation. The effects of an n-butanol extract on the induction of apoptosis were investigated by flow cytometry and mitochondrial cytochrome C-releasing apoptosis assay. Additionally, real-time PCR was employed to confirm apoptosis initiation. Further, qualitative estimation of the active constituent of was done by gas chromatography-mass spectroscopy (GC-MS). : The cell viability study revealed that the n-butanol extract of demonstrated potent cytotoxicity against HepG2 cancer cells, with an IC50 value of 56.76 μg/mL. Cell morphology with dual staining of acridine orange (AO)-ethidium bromide (EB) showed an increase in orange/red nuclei due to cell death by n-butanol extract compared to control cells. Apoptosis, as the mode of cell death, was also confirmed by the higher release of cytochrome C from mitochondria, the increased expression of caspase-3 and bax, along with down regulation of Bcl-2. These findings conclude that is a cause of hepatic cancer cell death through apoptosis and a potential natural source suggesting furthermore investigation of its active compounds that are responsible for these observed activities.
Amal A Alotaibi, Asmatanzeem Bepari, Rasha Assad Assiri, Shaik Kalimulla Niazi, Sreenivasa Nayaka, Muthuraj Rudrappa, Shashiraj Kareyellapa Nagaraja, Meghashyama Prabhakara Bhat

2988 related Products with: Exhibits Anti-Oncogenic Effect in Hepatocellular Carcinoma, HepG2 Cancer Cell Line by Bcl-2 Mediated Apoptotic Pathway and Mitochondrial Cytochrome C Release.

1 mL1 mL100ug Lyophilized

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#34563002   2021/09/03 To Up

Liver Cirrhosis and Hepatocellular Carcinoma Diagnosed from Chylothorax: A Case Report.

A 71-year-old man visited our hospital with dyspnea and left pleural effusion. Left pleural effusion was diagnosed as chylothorax by thoracentesis. He had no history of trauma or surgery, and there were no findings of malignant lymphoma or thrombosis. Furthermore, he was diagnosed with liver cirrhosis and hepatocellular carcinoma by computed tomography and hematological examinations, and the chylothorax was considered to be caused by liver cirrhosis. We report a review of the literature with this case since it is relatively rare for cirrhosis and hepatocellular carcinoma diagnosed from chylothorax.
Kenta Ito, Yoshimasa Hachisu, Mitsuhiko Shibasaki, Kazuma Ezawa, Hiroshi Iwashita, Asuka Jingu, Hirotaka Arai, Takeo Horie, Atsushi Takise

1805 related Products with: Liver Cirrhosis and Hepatocellular Carcinoma Diagnosed from Chylothorax: A Case Report.



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#34562836   2020/10/17 To Up

Medical gas plasma promotes blood coagulation via platelet activation.

Major blood loss still is a risk factor during surgery. Electrocauterization often is used for necrotizing the tissue and thereby halts bleeding (hemostasis). However, the carbonized tissue is prone to falling off, putting patients at risk of severe side effects, such as dangerous internal bleeding many hours after surgery. We have developed a medical gas plasma jet technology as an alternative to electrocauterization and investigated its hemostatic (blood clotting) effects and mechanisms of action using whole human blood. The gas plasma efficiently coagulated anticoagulated donor blood, which resulted from the local lysis of red blood cells (hemolysis). Image cytometry further showed enhanced platelet aggregation. Gas plasmas release reactive oxygen species (ROS), but neither scavenging of long-lived ROS nor addition of chemically-generated ROS were able to abrogate or recapitulate the gas plasma effect, respectively. However, platelet activation was markedly impaired in platelet-rich plasma when compared to gas plasma-treated whole blood that moreover contained significant amounts of hemoglobin indicative of red blood cell lysis (hemolysis). Finally, incubation of whole blood with concentration-matched hemolysates phenocopied the gas plasmas-mediated platelet activation. These results will spur the translation of plasma systems for hemolysis into clinical practice.
Sander Bekeschus, Broder Poschkamp, Julia van der Linde

2582 related Products with: Medical gas plasma promotes blood coagulation via platelet activation.

100 ug/vialBox of 10 vials for 500 m1UnitEach1100 ug/vial100ug/vial100 ug/vial100.00 ug

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#34562738   2021/09/17 To Up

Effect of ultrasound on the properties of rice bran protein and its chlorogenic acid complex.

Ultrasound technology was used to treat rice bran protein (RBP), and the structural and functional properties of ultrasonically treated RBP (URBP) and its chlorogenic acid (CA) complex were studied. When ultrasonic power of 200 W was applied for 10 min, the maximum emission peak λ of the URBP-CA complex in the fluorescence spectrum was red-shifted by 3.6 nm compared to that of the untreated complex. The atomic force microscope (AFM) analysis indicated that the surface roughness of the complex was minimized (3.89 nm) at the ultrasonic power of 200 W and treatment time of 10 min. Under these conditions, the surface hydrophobicity (H) was 1730, the contents of the α-helix and β-sheet in the complex were 2.97% and 6.17% lower than those in the untreated sample, respectively, the particle size decreased from 106 nm to 18.2 nm, and the absolute value of the zeta-potential increased by 11.0 mV. Therefore, ultrasonic treatment and the addition of CA changed the structural and functional properties of RBP. Moreover, when ultrasonic power of 200 W was applied for 10 min, the solubility, emulsifying activity index (EAI), and emulsion stability index (ESI) were 68%, 126 m/g, and 37 min, respectively.
Tong Wang, Xing Chen, Weining Wang, Liqi Wang, Lianzhou Jiang, Dianyu Yu, Fengying Xie

2510 related Products with: Effect of ultrasound on the properties of rice bran protein and its chlorogenic acid complex.

1 5 G100 101mg1 mg10 25 ml 100 U

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#34562734   2021/09/16 To Up

Roles of free radical on the formation of acetaldehyde in model wine solutions under different ultrasound parameters: A key bridge-link compound for red wine coloration during ageing.

In order to explore the effects of ultrasound on the formation of acetaldehyde and its mechanism in model wine solutions, ultrasound conditions and free radicals were investigated by response surface methodology and electron paramagnetic resonance spectroscopy (EPR), respectively. The results indicate that ultrasound does induce the production of acetaldehyde with the maximum amount under the conditions of ultrasound power density 0.2 W/cm, 48 min and 32 °C. The hydroxyl radicals and the 1-hydroxyethyl free radicals are the main initiator and precursor for acetaldehyde, respectively. Furthermore, the stronger the 1-hydroxyethyl free radicals captured by EPR, the lower the formation of acetaldehyde. In addition, the content of Feand ethanol also exerted a certain influence on the acetaldehyde formation. In conclusion, ultrasound does promote the production of acetaldehyde in the model wine solutions, which is beneficial for well understanding the mechanism of ultrasound in modifying the wine color and accelerating ageing.
Zhen-Dan Xue, Qing-An Zhang, Hong-Rong Zheng

2614 related Products with: Roles of free radical on the formation of acetaldehyde in model wine solutions under different ultrasound parameters: A key bridge-link compound for red wine coloration during ageing.

1100 μg96 Tests 1 G

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#34562702   2021/09/20 To Up

SuFEx modification of silk fibroin silicon aerogel and its adsorption behavior and antibacterial performance.

A silk fibroin silicon-based composite aerogel (SSA) has been modified via a SuFEx reaction for application in the adsorption of anionic pollutants and antimicrobials in water. The tyrosine fragment in the silk fibroin was modified by a high yielding SuFEx click reaction. A quaternary ammonium salt functionality was introduced into the silk fibroin protein and the modified silk fibroin protein was crosslinked with tetraethyl orthosilicate. The aerogel was then prepared by freeze-drying. The aerogel obtained has biocompatibility and biodegradability properties. Four types of dyes (Methyl orange, Rhodamine B, Methylene blue and Acid red) were applied as targets and the saturated adsorption amounts were calculated. The adsorption behavior of the dyes towards SSA was studied by fitting Langmuir and Freundlich adsorption models. A pseudo-first order kinetic model and a pseudo-second order kinetic model were used to study the kinetics of the adsorption process. After 6 cycles, the removal rate of methyl orange by SSA remained at 81.25%. The adsorption capacity and anti-interference ability of SSA on some other polluting anions such as PO and CrO were also measured and the efficiency adsorption reached up to 70.94% and 77.91%, respectively. The antibacterial effect of SSA was evaluated with Escherichia coli and Staphylococcus aureus as representative examples.
Yutao Zhu, Peiyang Gu, Haibo Wan, Shiyan Zhou, Jinghui He, Hua Li, Najun Li, Qingfeng Xu, Jianmei Lu

1675 related Products with: SuFEx modification of silk fibroin silicon aerogel and its adsorption behavior and antibacterial performance.

100ug1 ml10 mg1000 tests500 mg25 mg10 mg100ug50 ug 25 mg 5 G 5 G

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#34562506   2021/09/22 To Up

The involvement of TRPV4 on the hypoxia-induced oxidative neurotoxicity and apoptosis in a neuronal cell line: Protective role of melatonin.

The hypoxia (HYPX)-mediated excessive generation of mitochondrial free reactive oxygen species (mROS) and the overload Ca influx via the inhibition of TRPV4 are controlled by the treatment of antioxidants. However, the molecular mechanisms underlying melatonin (MLT)'s neuroprotection remains elusive. We investigated the role of MLT via modulation of TRPV4 on oxidative neurodegeneration and death in SH-SY5Y neuronal cells. The SH-SY5Y cells were divided into five groups as follows: control, MLT (1 mM for 2 h), HYPX (200 μM CoCl for 24 h), HYPX + MLT, and HYPX + TRPV4 blockers (ruthenium red-1 μM for 30 min). The HYPX caused to the increase of TRPV4 current density and overload Ca influx with an increase of mitochondrial membrane potential and mROS generation. The changes were not observed in the absence of TRPV4. When HYPX exposure and TRPV4 agonist (GSK1016790A)-induced TRPV4 activity were inhibited by the treatment of ruthenium red or MLT, the increase of mROS, lipid peroxidation, apoptosis, Zn concentrations, TRPV4, caspase -3, caspase -9, Bax, and Bcl-2 expressions were restored via upregulation of reduced glutathione, glutathione peroxidase and total antioxidant status. The levels of apoptosis and cell death in the cells were enriched with increases of caspase -3 and -9 activations, although they were decreased by MLT treatment. In conclusion, the treatment of MLT modulates HYPX-mediated mROS, apoptosis, and TRPV4-mediated overload Ca influx and may provide an avenue for protecting HYPX-mediated neurological diseases associated with the increase of mROS, Ca, and Zn concentration.
Ahmet Özşimşek, Mustafa Nazıroğlu

1807 related Products with: The involvement of TRPV4 on the hypoxia-induced oxidative neurotoxicity and apoptosis in a neuronal cell line: Protective role of melatonin.

11 mL1 mL96 assays

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#34562494   2021/09/22 To Up

First reports of envenoming by South American water snakes Helicops angulatus and Hydrops triangularis from Bolivian Amazon: A one-year prospective study of non-front-fanged colubroid snakebites.

Although snakebite incidence is underestimated in Bolivia, the Amazon region presents the highest incidence of these accidents. The local effects of bites by some non-front-fanged colubroid (NFFC) snakes are usually confused with that of viperids, resulting in the improper use of antivenoms and medications. Since there is scarce information on clinical treatment and management of NFFC bites from Bolivian Amazon, we conducted a prospective study of NFFC snakebites by reviewing the records of patients admitted with a snakebite diagnosis at Hospital Central Ivirgarzama, Bolivia. Snakebites were recorded for 12 months (December 2019-November 2020), including information about the sex and age of the patient, snakebite date, and treatment. Eight (5.7%) of 152 patients were bitten by NFFC Helicops angulatus, Hydrops triangularis, and Erythrolamprus sp. Our results showed that 5/7 patients had prolonged clotting time and INR, as well as local edema and mild pain, suggesting systemic envenoming. Previously non-documented mild coagulopathy was observed for H. angulatus and H. triangularis bites. In some cases, incorrect first-aid measures, and inappropriate use of bothropic/lachesic antivenom were administrated. All the patients received supportive therapy and antihistamine drugs. Unsupported use of non-evidence-based treatments for snakebites such as corticoids, non-steroidal anti-inflammatory drugs (NSAIDs), and prophylactic antibiotic prescription were recorded. In conclusion, we describe the first formally documented snakebite cases produced by NFFC from Bolivia, highlighting the urgent need for training of the medical team in the snake identification, clinical management of snakebite, and the existence of a human-snake conflict involving NFFC species.
Huber Villca-Corani, Beatriz Nieto-Ariza, Raúl León, José A Rocabado, Jean-Philippe Chippaux, Félix A Urra

1173 related Products with: First reports of envenoming by South American water snakes Helicops angulatus and Hydrops triangularis from Bolivian Amazon: A one-year prospective study of non-front-fanged colubroid snakebites.

100ug5mg25 mg 5 G100.00 ul 5 G1 g0,5 ML96 wells (1 kit)100ug100ul

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#34562440   2021/09/22 To Up

Rutin-Zn(II) complex promotes bone formation - A concise assessment in human dental pulp stem cells and zebrafish.

To assess the molecular role of Rutin and rutin-Zn(II) complex on osteoblast differentiation and mineralization in human dental pulp cells and in vivo zebra fish models. The biocompatibility of the rutin-Zn(II) complex was determined using MTT and chick embryotoxicity assays. Alizarin red staining and ALP measurements were performed to study the osteogenic role of Rutin and rutin-Zn(II) complex at the cellular level in hDPSCs. At molecular level, following rutin and rutin-Zn(II) exposure, the mRNA expression profile of osteoblast markers such Runx2, type 1 col, OC, and ON were investigated. In addition to this, the expression of negative regulators of osteoblast development such Smad7, Smurf1, and HDAC7 was studied by Realtime RT-PCR analysis. The osteogenic role of prepared complex under in vivo was studied by an in-house zebrafish scale model followed by osteoblast differentiation markers expression profiling and Ca:P level measurement by ICP-MS. Rutin and the rutin-Zn(II) complex were found to be non-toxic till 10μM and increased the expression of osteoblast differentiation marker genes. It also enhanced calcium deposition in both in vitro and in vivo models. Osteogenic property of rutin-Zn(II) in hDPSCs was found be mediated by Smad7, Smurf1, and HDAC7 and enhancing Runx2 expression. Our study warrants the possible use of rutin-Zn(II) as naïve agent or in combination with other bone scaffolding systems/materials for bone tissue engineering applications.
Selvaraj Vimalraj, Sekaran Saravanan, Raghunandhakumar Subramanian

2136 related Products with: Rutin-Zn(II) complex promotes bone formation - A concise assessment in human dental pulp stem cells and zebrafish.

1 mg10 ug1.00 flask50 ul1.00 flask100 ul100 μg100 μg100 μg100 μg100 μg

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