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Search results for: SCF

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#33630610   2021/02/25 To Up

Second-Order Orbital Optimization with Large Active Spaces Using Adaptive Sampling Configuration Interaction (ASCI) and Its Application to Molecular Geometry Optimization.

Recently, selected configuration interaction (SCI) methods that enable calculations with several tens of active orbitals have been developed. With the SCI subspace embedded in the mean field, molecular orbitals with an accuracy comparable to that of the complete active space self-consistent field method can be obtained. Here, we implement the analytical gradient theory for the single-state adaptive sampling CI (ASCI) SCF method to enable molecular geometry optimization. The resulting analytical gradient is inherently approximate due to the dependence on the sampled determinants, but its accuracy was sufficient for performing geometry optimizations with large active spaces. To obtain the tight convergence needed for accurate analytical gradients, we combine the augmented Hessian (AH) and Werner-Meyer-Knowles (WMK) second-order orbital optimization methods with the ASCI-SCF method. We test these algorithms for orbital and geometry optimizations, demonstrate applications of the geometry optimizations of polyacenes and periacenes, and discuss the geometric dependence of the characteristics of singlet ASCI wave functions.
Jae Woo Park

2700 related Products with: Second-Order Orbital Optimization with Large Active Spaces Using Adaptive Sampling Configuration Interaction (ASCI) and Its Application to Molecular Geometry Optimization.

100ul 100ul0.1ml (1.3mg/ml) 100ul100ug Lyophilized 100ul1 Set

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#33627868   2021/02/24 To Up

A mechanosensitive peri-arteriolar niche for osteogenesis and lymphopoiesis.

Stromal cells in adult bone marrow that express leptin receptor (LEPR) are a critical source of growth factors, including stem cell factor (SCF), for the maintenance of haematopoietic stem cells and early restricted progenitors. LEPR cells are heterogeneous, including skeletal stem cells and osteogenic and adipogenic progenitors, although few markers have been available to distinguish these subsets or to compare their functions. Here we show that expression of an osteogenic growth factor, osteolectin, distinguishes peri-arteriolar LEPR cells poised to undergo osteogenesis from peri-sinusoidal LEPR cells poised to undergo adipogenesis (but retaining osteogenic potential). Peri-arteriolar LEPRosteolectin cells are rapidly dividing, short-lived osteogenic progenitors that increase in number after fracture and are depleted during ageing. Deletion of Scf from adult osteolectin cells did not affect the maintenance of haematopoietic stem cells or most restricted progenitors but depleted common lymphoid progenitors, impairing lymphopoiesis, bacterial clearance, and survival after acute bacterial infection. Peri-arteriolar osteolectin cell maintenance required mechanical stimulation. Voluntary running increased, whereas hindlimb unloading decreased, the frequencies of peri-arteriolar osteolectin cells and common lymphoid progenitors. Deletion of the mechanosensitive ion channel PIEZO1 from osteolectin cells depleted osteolectin cells and common lymphoid progenitors. These results show that a peri-arteriolar niche for osteogenesis and lymphopoiesis in bone marrow is maintained by mechanical stimulation and depleted during ageing.
Bo Shen, Alpaslan Tasdogan, Jessalyn M Ubellacker, Jingzhu Zhang, Elena D Nosyreva, Liming Du, Malea M Murphy, Shuiqing Hu, Yating Yi, Nergis Kara, Xin Liu, Shay Guela, Yuemeng Jia, Vijayashree Ramesh, Claire Embree, Evann C Mitchell, Yunduo C Zhao, Lining A Ju, Zhao Hu, Genevieve M Crane, Zhiyu Zhao, Ruhma Syeda, Sean J Morrison

2005 related Products with: A mechanosensitive peri-arteriolar niche for osteogenesis and lymphopoiesis.

50 ug 1000 tests100 tests100μg 1 G1 ml25 mg10 mg0.1 mg100 mg2.5 mg

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#33626085   2021/02/24 To Up

Comparisons of adipogenesis- and lipid metabolism-related gene expression levels in muscle, adipose tissue and liver from Wagyu-cross and Holstein steers.

The intramuscular fat (IMF) content and fatty acid composition are important meat quality traits that are mostly affected by the cattle breed. Muscle, adipose tissue and liver are important organs involved in the development of intramuscular adipose tissue. Thus, we hypothesized that there were marked differences in the adipogenesis and lipid metabolism of these tissues between Wagyu-cross and Holstein steers during the finishing phases. To test this hypothesis, we analyzed the expression levels of adipogenesis- and lipid metabolism-related genes in longissimus muscle (LM), subcutaneous fat (SCF) and liver from Wagyu-cross and Holstein steers at 26 months of age. The IMF content and fatty acid profile of LM were determined. Wagyu-cross steers had a higher IMF content and MUFA percentages in the LM than Holstein steers (P<0.05). The relative expression of FGF2, COL1A1, SREBP1c, SCD1, GRP78 and LEP was greater in the LM of Wagyu-cross steers than in Holstein steers (P<0.05). In contrast, Holstein steer SCF had higher (P<0.05) mRNA expression levels of FABP4 and ADIPOQ than Wagyu-cross steers. In the liver, the expression of SREBP1c and GRP78 in Wagyu-cross steers was significantly higher than that in Holstein steers (P<0.05). The results demonstrate that both intramuscular adipogenesis and fibrogenesis are enhanced in Wagyu-cross steers compared with Holstein steers during the finishing phase and that IMF deposition is positively correlated with the maturity of SCF and hepatic lipid accumulation in Wagyu-cross steers.
Li Liu, Peili Cao, Lupei Zhang, Meiyu Qi, Liang Wang, Zhongqiu Li, Guang Shao, Liyan Ding, Xiuhua Zhao, Xiaochuan Zhao, Shanshan Xu, Haifeng Zhang, Jinbao Chai, Mengmeng Yue, Genlin Wang, Di Liu, Fang Sun

2572 related Products with: Comparisons of adipogenesis- and lipid metabolism-related gene expression levels in muscle, adipose tissue and liver from Wagyu-cross and Holstein steers.

96 wells100.00 ul100ul96 wells1 g100ug5mg96 wells (1 kit)100 mg200ug300 units

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#33619702   2021/02/22 To Up

Correction to: SCF Improves In Vitro Differentiation of SSCs Through Transcriptionally Up-regulating PRTM1, STRA8, c-KIT, PIWIL2, and OCT4 Genes.


Mahnaz Nasimi, Seyed Gholam Ali Jorsaraei, Esmail Fattahi, Maryam Gholamitabar Tabari, Ebrahim Zabihi Neyshaburi

1916 related Products with: Correction to: SCF Improves In Vitro Differentiation of SSCs Through Transcriptionally Up-regulating PRTM1, STRA8, c-KIT, PIWIL2, and OCT4 Genes.

2 Pieces/Box10mg100 μg50 ug96 tests1mg5 mg100ug Lyophilized1 g

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#33618531   // To Up

Eosinophil cationic protein: A new diagnostic biomarker in coronary slow flow phenomenon.

This study has investigated the role of eosinophil cationic protein (ECP), released by eosinophils, in the coronary slow flow phenomenon.
K Soylu, M Akcay, G Aksan, O Gedikli, N Gokay

1486 related Products with: Eosinophil cationic protein: A new diagnostic biomarker in coronary slow flow phenomenon.

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#33618520   2021/02/24 To Up

Cullin7 in tumor development: a novel potential anti-cancer target.

As a core scaffold protein, Cullin7 (Cul7) forms Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complexes with the regulator of cullins-1 (ROC1), S-phase kinase associated protein 1 (Skp1) and F-Box, and WD repeat domain containing 8 (Fbxw8). Alternatively, Cul7 can form a CRL7SMU1 complex with suppressor of Mec-8 and Unc-52 protein homolog (SMU1), damage-specific DNA binding protein 1 (DDB1), and ring finger protein 40 (RNF40), to promote cell growth. The mutations of Cul7 cause the 3-M dwarf syndrome, indicating Cul7 plays an important role in growth and development in humans and mice. Moreover, Cul7 regulates cell transformation, tumor protein p53 activity, cell senescence, and apoptosis, mutations in Cul7 are also involved in the development of tumors, indicating the characteristics of an oncogene. Cul7 is highly expressed in breast cancer, lung cancer, hepatocellular carcinoma, pancreatic cancer, ovarian cancer, and other malignant tumors where Cul7 promotes tumor development, cell transformation, and cell survival by regulating complex signaling pathways associated with protein degradation. In this review, we discuss the roles of Cul7 in malignant tumor development and its involvement in oncogenic signaling. We finally discuss the potential of Cul7 as a potential significant anti-cancer target.
Yumiao Li, Aiming Zang, Jiejun Fu, Youchao Jia

2353 related Products with: Cullin7 in tumor development: a novel potential anti-cancer target.

0.1ml (1mg/ml)0.1ml0.1ml

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#33612443   2021/02/18 To Up

[Onco-TESE and testicular cancer].

Fertility preservation is essential before cancer treatment. When ejaculated sperm preservation is not possible, testicular tissue can be surgically collected by Onco-TESE technic (Oncological Testicular Sperm Extraction) to isolate sperm. We report on our experience with Onco-TESE in testicular cancer patients at the Rouen University Hospital.
A Giwerc, A Chebbi, H Dupuis, H Chiavelli, J-N Cornu, C Pfister, A Safsaf, N Rives, L Sibert

1559 related Products with: [Onco-TESE and testicular cancer].

100ug5mg

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#33607891   // To Up

Polymer brushes with reversibly tunable grafting density.

We propose a novel class of responsive polymer brushes, where the effective grafting density can be controlled by external stimuli. This is achieved by using end-grafted polymer chains that have an affinity to the substrate. For sufficiently strong surface interactions, a fraction of chains condenses into a near-surface layer, while the remaining ones form the outer brush. The dense layer and the more tenuous outer brush can be seen as coexisting microphases. The effective grafting density of the outer brush is controlled by the adsorption strength and can be changed reversibly and in a controlled way as a response to changes in environmental parameters. The effect is demonstrated by numerical self-consistent field calculations and analyzed by scaling arguments. Since the thickness of the denser layer is about a few monomer sizes, its capacity to form a microphase is limited by the product of the brush chain length and the grafting density. We explore the range of chain lengths and grafting densities where the effect is most pronounced. In this range, the SCF studies suggest that individual chains inside the brush show large rapid fluctuations between two states that are separated by only a small free energy barrier. The behavior of the brush as a whole, however, does not reflect these large fluctuations, and the effective grafting density varies smoothly as a function of the control parameters.
Leonid I Klushin, Alexander M Skvortsov, Alexey A Polotsky, Anna S Ivanova, Friederike Schmid

2537 related Products with: Polymer brushes with reversibly tunable grafting density.

100ug Lyophilized100ug Lyophilized1 mg50 50ul100ug Lyophilized4 Sample Kit500 Units

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#33603745   2021/02/02 To Up

Glutamine Deprivation Promotes the Generation and Mobilization of MDSCs by Enhancing Expression of G-CSF and GM-CSF.

Solid tumors are often challenged by hypoxic and nutrient-deprived tumor microenvironments (TME) as tumors progress, due to limited perfusion and rapid nutrient consumption. While cancer cells can demonstrate the ability to survive in nutrient-deprived conditions through multiple intrinsic alterations, it is poorly understood how nutrient-deprived cancer cells co-opt the TME to promote cancer cell survival and tumor progression. In the present study, we found that glutamine deprivation markedly potentiated the expression of G-CSF and GM-CSF in mouse mammary cancer cells. The IRE1α-JNK pathway, which is activated by glutamine starvation, was found to be important for the upregulation of these cytokines. G-CSF and GM-CSF are well-known facilitators of myelopoiesis and mobilization of hematopoietic progenitor cells (HPC). Consistently, as tumors progressed, we found that several myeloid HPC compartments were gradually decreased in the bone marrow but were significantly increased in the spleen. Mechanistically, the HPC-maintaining capacity of the bone marrow was significantly impaired in tumor-bearing mice, with lower expression of HPC maintaining genes (i.e., CXCL12, SCF, ANGPT1, and VCAM1), and reduced levels of mesenchymal stem cells and CXCL12-producing cells. Furthermore, the mobilized HPCs that displayed the capacity for myelopoiesis were also found to accumulate in tumor tissue. Tumor-infiltrating HPCs were highly proliferative and served as important sources of immunosuppressive myeloid-derived suppressor cells (MDSCs) in the TME. Our work has identified an important role for glutamine starvation in regulating the expression of G-CSF and GM-CSF, and in facilitating the generation of immunosuppressive MDSCs in breast cancer.
Hong-Wei Sun, Wen-Chao Wu, Hai-Tian Chen, Yi-Tuo Xu, Yan-Yan Yang, Jing Chen, Xing-Juan Yu, Zilian Wang, Ze-Yu Shuang, Limin Zheng

1003 related Products with: Glutamine Deprivation Promotes the Generation and Mobilization of MDSCs by Enhancing Expression of G-CSF and GM-CSF.

100ul100ug10 mg100ug1000 tests500 mg25 mg10 mg 5 G100ul200 25 mg

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