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Search results for: Recombinant Human IL-4

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#33328477   2020/12/16 To Up

Eosinophils improve cardiac function after myocardial infarction.

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Jing Liu, Chongzhe Yang, Tianxiao Liu, Zhiyong Deng, Wenqian Fang, Xian Zhang, Jie Li, Qin Huang, Conglin Liu, Yunzhe Wang, Dafeng Yang, Galina K Sukhova, Jes S Lindholt, Axel Diederichsen, Lars M Rasmussen, Dazhu Li, Gail Newton, Francis W Luscinskas, Lijun Liu, Peter Libby, Jing Wang, Junli Guo, Guo-Ping Shi

2432 related Products with: Eosinophils improve cardiac function after myocardial infarction.

200.00 ug100ug Lyophilized 1 kit(s) 1 kit(96 Wells)200 10 1 mg100ug Lyophilized100ug Lyophilized200.00 ug100ug Lyophilized10

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#33198383   2020/11/12 To Up

A Novel HGF/SF Receptor (MET) Agonist Transiently Delays the Disease Progression in an Amyotrophic Lateral Sclerosis Mouse Model by Promoting Neuronal Survival and Dampening the Immune Dysregulation.

Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease with no effective treatment. The Hepatocyte Growth Factor/Scatter Factor (HGF/SF), through its receptor MET, is one of the most potent survival-promoting factors for motor neurons (MN) and is known as a modulator of immune cell function. We recently developed a novel recombinant MET agonist optimized for therapy, designated K1K1. K1K1 was ten times more potent than HGF/SF in preventing MN loss in an in vitro model of ALS. Treatments with K1K1 delayed the onset of muscular impairment and reduced MN loss and skeletal muscle denervation of superoxide dismutase 1 G93A (SOD1G93A) mice. This effect was associated with increased levels of phospho-extracellular signal-related kinase (pERK) in the spinal cord and sciatic nerves and the activation of non-myelinating Schwann cells. Moreover, reduced activated microglia and astroglia, lower T cells infiltration and increased interleukin 4 (IL4) levels were found in the lumbar spinal cord of K1K1 treated mice. K1K1 treatment also prevented the infiltration of T cells in skeletal muscle of SOD1G93A mice. All these protective effects were lost on long-term treatment suggesting a mechanism of drug tolerance. These data provide a rational justification for further exploring the long-term loss of K1K1 efficacy in the perspective of providing a potential treatment for ALS.
Antonio Vallarola, Massimo Tortarolo, Roberta De Gioia, Luisa Iamele, Hugo de Jonge, Giovanni de Nola, Enrica Bovio, Laura Pasetto, Valentina Bonetto, Mattia Freschi, Caterina Bendotti, Ermanno Gherardi

2688 related Products with: A Novel HGF/SF Receptor (MET) Agonist Transiently Delays the Disease Progression in an Amyotrophic Lateral Sclerosis Mouse Model by Promoting Neuronal Survival and Dampening the Immune Dysregulation.

100 μg500 100ug100 μg100ug

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#33193307   2020/10/23 To Up

Crosstalk Between Dermal Fibroblasts and Dendritic Cells During Dengue Virus Infection.

Dengue virus infection (DENV-2) is transmitted by infected mosquitoes the skin, where many dermal and epidermal cells are potentially susceptible to infection. Most of the cells in an area of infection will establish an antiviral microenvironment to control viral replication. Although cumulative studies report permissive DENV-2 infection in dendritic cells, keratinocytes, and fibroblasts, among other cells also infected, little information is available regarding cell-to-cell crosstalk and the effect of this on the outcome of the infection. Therefore, our study focused on understanding the contribution of fibroblast and dendritic cell crosstalk to the control or promotion of dengue. Our results suggest that dendritic cells promote an antiviral state over fibroblasts by enhancing the production of type I interferon, but not proinflammatory cytokines. Infected and non-infected fibroblasts promoted partial dendritic cell maturation, and the fibroblast-matured cells were less permissive to infection and showed enhanced type I interferon production. We also observed that the soluble mediators produced by non-infected or Poly (I:C) transfected fibroblasts induced allogenic T cell proliferation, but mediators produced by DENV-2 infected fibroblasts inhibited this phenomenon. Additionally, the effects of fibroblast soluble mediators on CD14 monocytes were analyzed to assess whether they affected the differentiation of monocyte derived dendritic cells (moDC). Our data showed that mediators produced by infected fibroblasts induced variable levels of monocyte differentiation into dendritic cells, even in the presence of recombinant GM-CSF and IL-4. Cells with dendritic cell-like morphology appeared in the culture; however, flow cytometry analysis showed that the mediators did not fully downregulate CD14 nor did they upregulate CD1a. Our data revealed that fibroblast-dendritic cell crosstalk promoted an antiviral response mediated manly by type I interferons over fibroblasts. Furthermore, the maturation of dendritic cells and T cell proliferation were promoted, which was inhibited by DENV-2-induced mediators. Together, our results suggest that activation of the adaptive immune response is influenced by the crosstalk of skin resident cells and the intensity of innate immune responses established in the microenvironment of the infected skin.
Alfredo E Montes-Gómez, Julio García-Cordero, Edith Marcial-Juárez, Gaurav Shrivastava, Giovani Visoso-Carvajal, Francisco J Juárez-Delgado, Leopoldo Flores-Romo, Ma Carmen Sanchez-Torres, Leopoldo Santos-Argumedo, José Bustos-Arriaga, Leticia Cedillo-Barrón

1562 related Products with: Crosstalk Between Dermal Fibroblasts and Dendritic Cells During Dengue Virus Infection.

5001000 1.00 flask1.00 flask25100200 500 1.00 flask1000100μg100

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#32194549   2020/02/28 To Up

Impact of BAFF Blockade on Inflammation, Germinal Center Reaction and Effector B-Cells During Acute SIV Infection.

Memory B-cell dysfunctions and inefficient antibody response suggest germinal center (GC) impairments during HIV/SIV infection with possible contribution of overproduced B-cell activating factor (BAFF). To address this question, we compared proportions and functions of various B-cell subsets and follicular helper T-cells (T) in untreated (Placebo) and BR3-Fc treated (Treated) SIV-infected macaques. From day 2 post-infection (dpi), Treated macaques received one weekly injection of BR3-Fc molecule, a soluble BAFF antagonist, for 4 weeks. Whereas, the kinetics of CD4 T-cell loss and plasma viral loads were comparable in both groups, BAFF blockade delayed the peak of inflammatory cytokines (CXCL10, IFNα), impaired the renewal of plasmacytoid dendritic cells and fostered the decline of plasma CXCL13 titers after 14 dpi. In Treated macaques, proportions of total and naïve B-cells were reduced in blood and spleen whereas SIV-induced loss of marginal zone (MZ) B-cells was only accentuated in blood and terminal ileum. Proportions of spleen GC B-cells and T were similar in both groups, with CD8 T-cells and rare Foxp3 being present in spleen GC. Regardless of treatment, sorted T produced similar levels of IL21, CXCL13, and IFNγ but no IL2, IL4, or BAFF and exhibited similar capacities to support IgG production by autologous or heterologous B-cells. Consistently, most T were negative for BAFF-R and TACI. Higher proportions of resting and atypical (CD21) memory B-cells were present in Treated macaques compared to Placebo. In both groups, we found higher levels of BAFF-R expression on MZ and resting memory B-cells but low levels on atypical memory B-cells. TACI was present on 20-30% of MZ, resting and atypical memory B-cells in Placebo macaques. BAFF blockade decreased TACI expression on these B-cell subsets as well as titers of SIV-specific and vaccine-specific antibodies arguing for BAFF being mandatory for plasma cell survival. Irrespective of treatment, GC B-cells expressed BAFF-R at low level and were negative for TACI. In addition to key information on spleen BAFF-R and TACI expression, our data argue for BAFF contributing to the GC reaction in terminal ileum but being dispensable for the generation of atypical memory B-cells and GC reaction in spleen during T-dependent response against SIV.
Gwenoline Borhis, Maria Trovato, Hany M Ibrahim, Stephane Isnard, Roger Le Grand, Nathalie Bosquet, Yolande Richard

1019 related Products with: Impact of BAFF Blockade on Inflammation, Germinal Center Reaction and Effector B-Cells During Acute SIV Infection.

250 Units25 mg1mg2.00 flask15ml 1 kit(s) 8 Sample Kit500 Reactions 25 ml 100 µg

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#32086822   2020/03/10 To Up

IL-1β prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early-life rhinovirus infection in mice.

Early-life wheezing-associated respiratory infection with human rhinovirus (RV) is associated with asthma development. RV infection of 6-day-old immature mice causes mucous metaplasia and airway hyperresponsiveness which is associated with the expansion of IL-13-producing type 2 innate lymphoid cells (ILC2s) and dependent on IL-25 and IL-33. We examined regulation of this asthma-like phenotype by IL-1β.
Mingyuan Han, Tomoko Ishikawa, Jennifer R Bermick, Charu Rajput, Jing Lei, Adam M Goldsmith, Caitlin R Jarman, Julie Lee, J Kelley Bentley, Marc B Hershenson

1004 related Products with: IL-1β prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early-life rhinovirus infection in mice.

96T96T10 100 μg1 kit250ul96 tests104 Membranes/Box5mg4 Membranes/Box

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#31879800   2019/12/26 To Up

Sialic Acid-Engineered IL4-10 Fusion Protein is Bioactive and Rapidly Cleared from the Circulation.

Modulating sialylation of therapeutic glycoproteins may be used to influence their clearance and systemic exposure. We studied the effect of low and high sialylated IL4-10 fusion protein (IL4-10 FP) on in vitro and in vivo bioactivity and evaluated the effect of differential sialylation on pharmacokinetic parameters.
Cristine Steen-Louws, Peter Boross, Judith Prado, Jan Meeldijk, Jurgen B Langenhorst, Alwin D R Huitema, Marcel T den Hartog, Louis Boon, Floris P J G Lafeber, C Erik Hack, Niels Eijkelkamp, Jelena Popov-Celeketic

1361 related Products with: Sialic Acid-Engineered IL4-10 Fusion Protein is Bioactive and Rapidly Cleared from the Circulation.

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#31863534   2020/01/02 To Up

Targeting interleukin 4 receptor α: A new approach to the treatment of cutaneous autoimmune bullous diseases?

Bullous pemphigoid, mucous membrane pemphigoid, and pemphigus vulgaris are different cutaneous autoimmune blistering diseases, with complex pathogenic mechanisms. In all of them, a type-2 response is thought to have a central role. Interleukin 4 and Interleukin 13 are crucial cytokines in type-2 response. Treatment of these conditions is often challenging. Dupilumab, a recombinant fully human IgG4 monoclonal antibody with binding specificity to human interleukin-4 receptor IL-4Rα, has the potential to inhibit both IL-4 and IL-13. We propose IL-4Rα as a theoretical drug target for cutaneous autoimmune bullous diseases.
Roberto Russo, Emanuele Cozzani, Giulia Gasparini, Aurora Parodi

2930 related Products with: Targeting interleukin 4 receptor α: A new approach to the treatment of cutaneous autoimmune bullous diseases?

500 100ug Lyophilized96T100ug Lyophilized96 wells (1 kit)100ug Lyophilized600 Tests / Kit2.5 g100ug Lyophilized

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#31825972   2019/12/11 To Up

IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection.

Ebolavirus (EBOV) outbreaks, while sporadic, cause tremendous morbidity and mortality. No therapeutics or vaccines are currently licensed; however, a vaccine has shown promise in clinical trials. A critical step towards development of effective therapeutics is a better understanding of factors that govern host susceptibility to this pathogen. As macrophages are an important cell population targeted during virus replication, we explore the effect of cytokine polarization on macrophage infection.
Kai J Rogers, Bethany Brunton, Laura Mallinger, Dana Bohan, Kristina M Sevcik, Jing Chen, Natalie Ruggio, Wendy Maury

1297 related Products with: IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection.

200 1 mg200 200ug500 100ug Lyophilized100 mg 1L96 Tests n100 µg50ug100

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#31819584   2019/11/29 To Up

Interferon Kappa Is Up-Regulated in Psoriasis and It Up-Regulates Psoriasis-Associated Cytokines in vivo.

There is increased type I interferon signature in psoriasis patients. Interferon-kappa (IFN-κ) is a member of type I interferon family that is constitutively expressed by keratinocytes. In this study, we investigate whether IFN-κ is involved in psoriasis etiology.
Yuanyuan Li, Yueqi Song, Leqing Zhu, Xiao Wang, Bin Yang, Ping Lu, Quan Chen, Lianghua Bin, Liehua Deng

1847 related Products with: Interferon Kappa Is Up-Regulated in Psoriasis and It Up-Regulates Psoriasis-Associated Cytokines in vivo.

100ug Lyophilized0.1ml (1mg/ml)100ug Lyophilized1 mg100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized1 mg100ug Lyophilized

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