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#33850744   // To Up

Association of Zinc deficiency, oxidative stress and increased double-stranded DNA breaks in globozoospermic infertile patients and its implication for the assisted reproductive technique.

Sperm DNA fragmentation and its adverse impact on outcomes of assisted reproductive techniques (ART) in globozoospermic infertile patients has been previously reported. However, the association of Zinc element with DNA damage and intracytoplasmic sperm injection (ICSI) outcome in globozoospermic infertile patients remains unclear.
Lin Huang, Guanping Yao, Gelin Huang, Chuan Jiang, Li Li, Lu Liao, Guiping Yuan, Lijun Shang, Wenming Xu

1625 related Products with: Association of Zinc deficiency, oxidative stress and increased double-stranded DNA breaks in globozoospermic infertile patients and its implication for the assisted reproductive technique.

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#33759027   2021/03/23 To Up

The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants.

Cadmium (Cd), copper (Cu), and zinc (Zn) are among the most common heavy metals (HMs) present in polluted soils. While some HMs are required for key biological processes, they are toxic when present in excess. This toxicity damages plant health, decreases crop yields, and can impact human health via the food chain. For example, durum wheat is a staple food that is known to accumulate Cd when grown on polluted soils. Plant response to HM stress is complex and involves several transcription factors (TFs) among which members of the ERF family. Although roles of SHINE-type ERF transcription factors in abiotic stress tolerance have been thoroughly investigated, there is little information concerning their role in HM stress tolerance. In the present study, we investigated the role of durum wheat TdSHN1 TF in HM response and tolerance. Results showed that TdSHN1 expression was strongly induced by Cd, Cu, and Zn in durum wheat seedlings. In addition, TdSHN1 gene promoter directed HM-inducible GUS gene expression in transgenic tobacco. Overexpression of TdSHN1 encoding cDNA in transgenic yeast and tobacco conferred Cd, Cu, and Zn tolerances. Interestingly, transgenic tobacco lines exhibited longer roots and greater biomass accumulation, retained more chlorophyll, and produced less ROS than WT plants, when subjected to excess HMs. In addition, transgenic tobacco lines had higher activities of ROS-scavenging enzymes (SOD and CAT) which might have contributed to their HM tolerance. This study suggested that TdSHN1 is a potential candidate for improving HM tolerance in plants and phytoremediation of HM-contaminated soils.
Rania Djemal, Habib Khoudi

2157 related Products with: The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants.

25 mg100ug100 mg 25 MG50 IU100ug9610 mg51000 200ul

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#33727057   2021/03/13 To Up

β-Hydroxybutyrate inhibits cardiac microvascular collagen 4 accumulation by attenuating oxidative stress in streptozotocin-induced diabetic rats and high glucose treated cells.

Accumulation of collagen 4 (COL4) and thickened basement membrane are features of diabetic cardiac microvascular fibrosis that may be induced by oxidative stress. The ketone body β-hydroxybutyrate exhibits various cardiovascular protective effects, however its mechanism remains to be clarified. In the current study, the effects of β-hydroxybutyrate on cardiac microvascular fibrosis and COL4 accumulation were evaluated in streptozotocin-induced diabetic rats and in high glucose (HG) treated human cardiac microvascular endothelial cells (HCMECs). Generations of inducible nitric oxide synthase (iNOS) and copper-zinc superoxide dismutase (Cu/Zn-SOD), and the amount of nitrotyrosine (NT) were measured in vivo and in vitro. Ten weeks of β-hydroxybutyrate treatment (160, 200 and 240 mg/kg/d) attenuated cardiac microvascular fibrosis and inhibited cardiac COL4 generation and microvascular distribution in diabetic rats. Furthermore, β-hydroxybutyrate promoted cardiac Cu/Zn-SOD generation and reduced NT content, without reducing iNOS generation in diabetic rats. In HCMECs, stimulation with HG induced excess generation of COL4 via peroxynitrite. β-Hydroxybutyrate treatment (2, 4, 6 mM) attenuated HG-stimulated COL4 accumulation in a concentration-dependent manner. Similarly, 4 mM β-hydroxybutyrate promoted Cu/Zn-SOD generation and reduced NT content, without affecting excess iNOS generation in HG-stimulated HCMECs. In conclusion, this study showed that β-hydroxybutyrate promoted Cu/Zn-SOD generation, reduced peroxynitrite and inhibited cardiac microvascular COL4 accumulation in diabetes.
Huanli Qi, Lihui Gu, Dongmei Xu, Kun Liu, Mingjie Zhou, Yu Wang, Xiujuan Wang, Yanning Li, Jinsheng Qi

1258 related Products with: β-Hydroxybutyrate inhibits cardiac microvascular collagen 4 accumulation by attenuating oxidative stress in streptozotocin-induced diabetic rats and high glucose treated cells.

1.00 flask400Tests1.00 flask96 assays2 Pieces/Box1.00 flask2 Pieces/Box4 Membranes/Box1x10e7 cells2 Pieces/Box

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#33663349   2021/03/04 To Up

Phenothiazine as novel human superoxide dismutase modulators: discovery, optimization, and biological evaluation.

Superoxide dismutases (SODs) are regarded as important antioxidants for protecting cells against damage arising from oxidative stress. Much research is focused on finding new chemicals with an ability to boost human SOD activity. In the research described herein a structure-based approach was used to identify new human Cu-Zn superoxide dismutase (SOD1) modulators based on previously reported plasmodium falciparum iron SOD inhibitors using induced fit docking and molecular dynamic (MD) protocols. The compound with the highest docking binding energy was selected for further structure simplification followed by structural similarity and MD in order to find a new activator/inhibitor scaffold of the SOD1 enzyme. According to the docking survey of the mentioned series, 1,4-bis(3-(1,4,8-trichloro-10Hphenothiazin-10-yl) propyl) piperazine (DS88) was the top scoring compound interacting with the SOD1 active site channel. Following structure simplification and similarity search, the most promising scaffold which is closely related to the phenothiazine antipsychotic class, was identified. Compared with the normal blood SOD1 activity, the percent of O production increased with trifluoperazine, while it decreased with the chlorpromazine. The molecular dynamic investigation shows that trifluoperazine exerts its SOD1 activating effect by stabilizing electrostatic loop while chlorpromazine employs SOD1 inhibition activity through repositioning of the electrostatic loop and increasing its distance from the catalytic metal site which diminished substrate specificity and catalytic activity of the SOD1 enzyme. The results identified the preferred region, orientation, and types of interaction for each activator or inhibitor compound.
Homa Azizian, Simin Forooghian, Arash Amanlou, Horacio Pérez-Sánchez, Massoud Amanlou

1934 related Products with: Phenothiazine as novel human superoxide dismutase modulators: discovery, optimization, and biological evaluation.

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#33653412   2021/03/02 To Up

Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway.

Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They are rich in flavonoids.
Xiang-Bo Jia, Quan Zhang, Lei Xu, Wen-Jian Yao, Li Wei

2904 related Products with: Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway.

2 Pieces/Box0.1 mg50 mg25 50 ul100 extractions25 101.00 flask

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#33622876   // To Up

Pre-Treatment of Pterostilbene Enhances HO-induced Cell Apoptosis Through Caspase-dependent Pathway in Human Keratinocyte Cells.

Hydrogen peroxide (HO) is one of the reactive oxygen species (ROS), which can induce apoptotic cell death in numerous cancer cells. Pterostilbene (PTE), a natural polyphenolic compound, induces cell apoptosis in many human cancer cells.
Yi-Ching Cheng, Po-Yuan Chen, Tzong-DER Way, Ching-Ling Cheng, Yi-Ping Huang, Te-Chun Hsia, Yu-Cheng Chou, Shu-Fen Peng

1182 related Products with: Pre-Treatment of Pterostilbene Enhances HO-induced Cell Apoptosis Through Caspase-dependent Pathway in Human Keratinocyte Cells.

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#33593447   2021/02/16 To Up

A new candidate vaccine for human brucellosis based on influenza viral vectors: a preliminary investigation for the development of an immunization schedule in a guinea pig model.

A new candidate vector vaccine against human brucellosis based on recombinant influenza viral vectors (rIVV) subtypes H5N1 expressing Brucella outer membrane protein (Omp) 16, L7/L12, Omp19 or Cu-Zn SOD proteins has been developed. This paper presents the results of the study of protection of the vaccine using on guinea pigs, including various options of administering, dose and frequency. Provided data of the novel vaccine candidate will contribute to its further movement into the preclinical stage study.
Dina Bugybayeva, Zhailaubay Kydyrbayev, Nadezhda Zinina, Nurika Assanzhanova, Bolat Yespembetov, Yerken Kozhamkulov, Kunsulu Zakarya, Sholpan Ryskeldinova, Kaissar Tabynov

2503 related Products with: A new candidate vaccine for human brucellosis based on influenza viral vectors: a preliminary investigation for the development of an immunization schedule in a guinea pig model.

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#33592447   2021/02/08 To Up

Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation.

The Zrt/Irt-like proteins (ZIP, SLC39A) and zinc transporters (ZnT, SLC30A) are the two major gene families responsible for the import/export of Zn and other metals. In this study, the mRNA expression levels and genetic variations of eight ZnTs and 14 ZIPs were identified in Crassostrea gigas after exposure to Zn, Cd, Cu, Hg, and Pb. Metal exposure induced reactive oxygen species (ROS) and malondialdehyde (MDA) accumulation and antioxidant enzyme expression. The expanded gene numbers of superoxide dismutase (SOD) in the oysters exhibited diverse expression under exposure to the five metals, and the contrasting expressions of both ZnTs and ZIPs under different metal exposures were observed, revealing their ion-specific responses. Zn and Cu have similar transporters and induce high expression levels of ZnT1, 2, 7, and 9 and ZIP1, 3, 6, 9, 10, 11, and 14. Pb induced high expression levels of ZIP7, and 13 and ZnT5, 6, and 7, which are mainly expressed in the endoplasmic reticulum (ER). Cd induced high expression levels of ZnT1, 2, and 7 and ZIP1, 6, 9, 10, 11, and 13. Hg exposure was found to have little effect on the ZIP and ZnT expression levels. Based on 3784 single nucleotide polymorphisms (SNPs) within the ZnTs and ZIPs, genetic association analysis for Zn accumulation was conducted on 427 oyster samples. The 38 SNPs, which were located within 12 genes, were identified to be associated with Zn content (p < 0.01), explaining the phenotypic variation from 1.61% to 3.37%. One nonsynonymous mutation and related haplotypes were identified within ZIP1, explaining 1.69% of the variation in Zn. Its high expression under Zn exposure revealed its important role in Zn transportation. To the best of our knowledge, this study is the first comprehensive investigation of the transportation mechanisms of ZIPs and ZnTs under different metal exposures and the genetic effect of Zn accumulation in oysters, and provides valuable biomarkers and genetic resources to evaluate environmental metal pollution.
Jie Meng, Wen-Xiong Wang, Li Li, Guofan Zhang

2827 related Products with: Accumulation of different metals in oyster Crassostrea gigas: Significance and specificity of SLC39A (ZIP) and SLC30A (ZnT) gene families and polymorphism variation.

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#33431776   // To Up

Protective Effect of Nervonic Acid Against 6-Hydroxydopamine-Induced Oxidative Stress in PC-12 Cells.

Increased oxidative stress in the human brain is observed in neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD), and is considered to be a major cause of progression of these disease states. A very long-chain fatty acid, nervonic acid (NA), is the main fatty acid found in various sphingolipid species in the central nervous system. NA plays an important role in forming the plasma membrane's lipid bilayer and in maintaining normal myelin function. In this study, we examined the neuroprotective effect of NA against rat pheochromocytoma (PC-12) cells stimulated by 6-hydroxydopamine (6-OHDA), which served as a cell model of PD. PC-12 cells were pre-treated with different concentrations of NA for 48 h then subsequently co-treated with NA and 6-OHDA for 48 h to induce cellular oxidative stress. Cell viability was significantly increased by pre-treatment with a very low concentration of NA. The level of malondialdehyde, a marker of lipid peroxidation, was significantly decreased in NA-treated cells. The expression levels of superoxide dismutases (Mn SOD and Cu/Zn SOD) and γ-glutamylcysteine synthetase (GCLC), responsible for the synthesis of glutathione, were significantly increased, indicating that pre-treatment with NA activated the cellular antioxidant defense system. These results suggest that NA may play a role as a neuroprotective mediator in the brain.
Hiroki Umemoto, Saika Yasugi, Shinji Tsuda, Minoru Yoda, Takashi Ishiguro, Naoko Kaba, Tatsuki Itoh

2317 related Products with: Protective Effect of Nervonic Acid Against 6-Hydroxydopamine-Induced Oxidative Stress in PC-12 Cells.

500 MG100 ul10 mg100ug Lyophilized1 ml 5 G5 mg- 25 G96 tests5 mg

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#33319813   2020/12/15 To Up

Oxidative stress as a hidden cost of attractiveness in postmenopausal women.

Perceived facial attractiveness, a putative marker of high biological fitness, is costly to maintain throughout a lifetime and may cause higher oxidative stress (OS). We investigated the association between the facial features of 97 postmenopausal women and their levels of OS biomarkers 8-hydroxy-2'-deoxyguanosine (8-OHdG), superoxide dismutase (Cu-Zn SOD), and thiobarbituric acid reactive substances (TBARS). In study 1, 966 judges rated the composites (facial averages) of women with higher OS as more attractive, healthier, younger, and less symmetric. In study 2, Geometric Morphometric analysis did not reveal significant differences in facial morphology depending on OS levels. In study 3, measured facial averageness and symmetry were weakly negatively related to 8-OHdG levels. Maintaining higher perceived facial attractiveness may be costly due to increased oxidative damage in the postmenopausal period. These costs may remain hidden during the reproductive period of life due to the protective mechanisms of oxidative shielding and revealed only after menopause when shielding has ceased.
Urszula M Marcinkowska, Anna Ziomkiewicz, Karel Kleisner, Andrzej Galbarczyk, Magdalena Klimek, Amelia Sancilio, Grazyna Jasienska, Richard G Bribiescas

1122 related Products with: Oxidative stress as a hidden cost of attractiveness in postmenopausal women.

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