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#34110479   2021/06/10 To Up

Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil.

Multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae (hvKp) clones have become a major threat to global public health. The clonal group 258 (CG258) is considered a high-risk CG and the K. pneumoniae strains belonging to it are often multi-resistant and to spread mainly in the hospital environment. This study aimed to characterize the antimicrobial resistance profile, virulence factors, and the clonal relationships among 13 K. pneumoniae strains belonging to CG258 from patients admitted to a tertiary hospital in Teresina, in the state of Piauí, northeastern Brazil. Ten strains were classified as MDR and three as extensively drug-resistant (XDR). Three different β-lactamase-encoding genes (bla, bla, and bla and six virulence genes (fimH, ycfM, mrkD, entB, ybtS, and kfu) were detected. Moreover, two hypermucoviscous K. pneumoniae strains and one capsular K-type 2 were found. Multilocus sequence typing analysis revealed ten different sequence types (STs) (ST14, ST17, ST20, ST29, ST45, ST101, ST268, ST1800, ST3995, and ST3996) belonging to CG258, being two (ST3995 and ST3996) described for the first time in this study.
Rafael Nakamura-Silva, Mariana Oliveira-Silva, João Pedro Rueda Furlan, Eliana Guedes Stehling, Carlos Eduardo Saraiva Miranda, André Pitondo-Silva

2000 related Products with: Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil.

1 G1mg96 wells

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#33995300   2021/04/30 To Up

Genotypic Characterization of Clinical spp. Isolates Collected From Patients With Suspected Community-Onset Sepsis, Sweden.

is a genus of Gram-negative bacteria known to be opportunistic pathogens that may cause a variety of infections in humans. Highly drug-resistant species, especially , have emerged rapidly and are becoming a major concern in clinical management. Although is considered the most important pathogen within the genus, the true clinical significance of the other species is likely underrecognized due to the inability of conventional microbiological methods to distinguish between the species leading to high rates of misidentification. Bacterial whole-genome sequencing (WGS) enables precise species identification and characterization that other technologies do not allow. Herein, we have characterized the diversity and traits of spp. in community-onset infections by WGS of clinical isolates ( = 105) collected during a prospective sepsis study in Sweden. The sequencing revealed that 32 of the 82 isolates (39.0%) initially identified as with routine microbiological methods based on cultures followed by matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF MS) had been misidentified. Of these, 23 were identified as and nine as other members of the complex. Comparisons of the number of resistance genes showed that significantly fewer resistance genes were detected in compared to and (both values of < 0.001). Moreover, a high proportion of the isolates within the complex were predicted to be genotypically multidrug-resistant (MDR; 79/84, 94.0%) in contrast to (3/16, 18.8%) and (0/4, 0.0%). All isolates predicted as genotypically MDR were found to harbor the combination of β-lactam, fosfomycin, and quinolone resistance markers. Multi-locus sequence typing (MLST) revealed a high diversity of sequence types among the spp. with ST14 (10.0%) and ST5429 (10.0%) as the most prevalent ones for , ST146 for (12.0%), and ST176 for (25.0%). In conclusion, the results from this study highlight the importance of using high-resolution genotypic methods for identification and characterization of clinical spp. isolates. Our findings indicate that infections caused by other members of the complex than are a more common clinical problem than previously described, mainly due to high rates of misidentifications.
Patricia Saxenborn, John Baxter, Andreas Tilevik, Magnus Fagerlind, Fredrik Dyrkell, Anna-Karin Pernestig, Helena Enroth, Diana Tilevik

2866 related Products with: Genotypic Characterization of Clinical spp. Isolates Collected From Patients With Suspected Community-Onset Sepsis, Sweden.



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#33958683   2021/05/06 To Up

Successful treatment of infective endocarditis due to pandrug-resistant Klebsiella pneumoniae with ceftazidime-avibactam and aztreonam.

Pandrug-resistant (PDR) K. pneumoniae refractory to conventional treatment has been reported worldwide, causing a huge burden on the healthcare system, patient safety and the economy. K. pneumoniae is a prominent opportunistic pathogen causing hospital-acquired and community-acquired infections, but is rarely associated with infective endocarditis. Currently, there are sparse data guiding the optimal regimen when commonly used antibiotics fail, notably for the treatment of endocarditis infections. Here we report our experience in treating a 40-year-old female with PDR K. pneumoniae infection of cardiovascular implantable electronic device (CIED) and right-sided infective endocarditis. Initial susceptibility testing of the incriminated pathogen showed an apparent susceptibility to colistin but the prolonged course of colistin, gentamicin and meropenem did not resolve the infection. However, the synergistic combinations of aztreonam with ceftazidime-avibactam was able to overcome resistance and clear the infection rapidly. Genome sequencing showed that the PDR K. pneumoniae isolate belongs to the international high-risk clone ST14. The isolate harbored genes encoding NDM-1, OXA-48, CTX-M-14b, SHV-28 and OXA-1, explaining resistance to all β-lactams, including carbapenems. It carried the armA gene conferring resistance to all clinically important aminoglycosides and had alterations in GyrA, ParC and MgrB, explaining resistance to ciprofloxacin and colistin.
Majed F Alghoribi, Moayad Alqurashi, Liliane Okdah, Bassam Alalwan, Yahya S AlHebaishi, Abdulmajeed Almalki, Maha A Alzayer, Abdulrahman A Alswaji, Michel Doumith, Mazin Barry

1834 related Products with: Successful treatment of infective endocarditis due to pandrug-resistant Klebsiella pneumoniae with ceftazidime-avibactam and aztreonam.

1200 100 G100 assays1 mL 500 G50 100 μg100 g1 module10 mg1 ml

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#33932364   2021/04/28 To Up

Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey.

A total of 1340 fresh fecal samples from farm and pet animals in Central Anatolia and the Middle Black Sea Region of Turkey were investigated using a PCR assay targeting the SSU rRNA of Blastocystis sp. An overall Blastocystis sp. prevalence of 19.4% (183/940) was found in farm animals, including cattle, sheep, water buffaloes, and chickens. Fecal samples of dogs, cats, and horses were negative. The highest prevalence of Blastocystis sp. was found in sheep (38.2%) among the farm animals. The SSU rRNA sequence analysis revealed two animal-specific subtypes, including ST10 in cattle and sheep and ST14 in water buffaloes. The zoonotic subtype ST7 was identified in chickens. Our results indicated a high prevalence of animal-specific subtypes in livestock and zoonotic subtype ST7 in chickens, highlighting the potential risk of chickens for zoonotic transmission of Blastocystis in the research area. This study is the first large-scale evaluation of Blastocystis in animal hosts in Turkey, and contributes to the molecular epidemiology and genetics of Blastocystis. Our results should be considered by authorities as an indication of the zoonotic importance of Blastocystis sp. and the need for surveillance in public health intervention programs.
Zuhal Onder, Alparslan Yildirim, Didem Pekmezci, Onder Duzlu, Gokmen Zafer Pekmezci, Arif Ciloglu, Emrah Simsek, Nesrin Delibasi Kokcu, Gamze Yetismis, Nuri Ercan, Abdullah Inci

1945 related Products with: Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey.

900 tests100 μg1 Set100 μg100ug Lyophilized100 μg10 100 24 tests

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#33910644   2021/04/28 To Up

Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics.

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is an important zoonotic agent worldwide. The aim of this work was to compare genetically 117 S. Typhimurium isolated from different sources over 30 years in Brazil using different genomics strategies.
Amanda Ap Seribelli, Patrick da Silva, Marcelo Ferreira da Cruz, Fernanda de Almeida, Miliane R Frazão, Marta I C Medeiros, Dália Dos P Rodrigues, Jalusa D Kich, Leandro de Jesus Benevides, Siomar de C Soares, Marc W Allard, Juliana Pfrimer Falcão

2901 related Products with: Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics.

100ug Lyophilized100ug Lyophilized0.1ml1 mg100ug Lyophilized100ug Lyophilized1 mg100ug Lyophilized

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#33904983   2021/04/27 To Up

Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.

Blastocystis sp. is an intestinal protist parasite commonly found in the feces of humans and animals worldwide. Blastocystis exhibits extensive genetic diversity and has been identified in humans and a variety of animals including other mammals and birds. Blastocystis subtypes do not exhibit strict host specificity which raises the possibility of zoonotic transmission through either direct contact or fecal contamination of food or water. However, reports detailing the subtypes and prevalence of Blastocystis in avian species are limited. Therefore, this study investigated the presence of Blastocystis in chickens by molecular characterization of the small subunit rRNA (SSU rRNA) gene. Fecal samples from 130 chickens were collected from local markets in Uberlândia and Belo Horizonte in the state of Minas Gerais, Brazil. To detect and identify subtypes of Blastocystis, a next-generation amplicon sequencing protocol was used. Forty-four of the 130 (33.8%) chickens examined were positive for Blastocystis. Blastocystis subtypes ST6 (23/130; 17.7%), ST7 (43/130; 33.1%), ST10 (1/130; 0.8%), ST14 (5/130; 3.8%), ST25 (1/130; 0.8%), and a novel subtype (ST29) (2/130; 1.5%) were observed. A nanopore sequencing strategy was used to obtain the near full-length SSU rRNA gene nucleotide sequence and validate novel subtype ST29. Mixed infections containing multiple subtypes were common and identified in 63.6% of Blastocystis-positive chickens. All positive samples contained one or both potentially zoonotic subtypes ST6 and ST7. The prevalence of Blastocystis in chickens was high, and molecular characterization mostly identified subtypes previously found in humans. Thus, chickens may be a source of human infection and environmental contamination.
Jenny G Maloney, Maria J R da Cunha, Aleksey Molokin, Márcia C Cury, Monica Santin

1947 related Products with: Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.



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#33820943   2021/04/05 To Up

Mutations in two component system (PhoPQ and PmrAB) in colistin resistant Klebsiella pneumoniae from North Indian tertiary care hospital.

Colistin resistance in Gram negative bacteria is mainly attributed to chromosomal mutations in Two Component Systems(TCS) PhoPQ and PmrAB and plasmid-borne genes(mcr and its variants). The aim of this study was to understand the molecular basis of colistin resistance in Klebsiella pneumoniae and determine clonal transmission, in a North Indian tertiary care hospital over a 2.5 year period. Antimicrobial susceptibility was determined by Vitek and colistin resistance was confirmed by broth microdilution. Carbapenemases(bla, bla, bla, bla, bla) and mcr-1 screening was done by PCR. Mutations in chromosomal genes mgrB, phoP, phoQ, pmrA, pmrB were analysed. Sequence typing was performed by Multilocus sequence typing(MLST). OXA-48 was detected in thirteen isolates while three isolates co-expressed OXA-48 and NDM. The mcr-1 gene was absent in all 16 isolates. Deleterious mutations in mgrB included insertion sequences IS903 and ISkpn26 and a premature stop codon. A total of 18 point mutations were identified in PhoPQ and PmrAB TCS; of which, novel mutations were reported in phoQ (K46E, L322V, D152N, F373L, R249G), pmrB (P159R) and pmrA (D149L). Six different sequence types ST231, ST147, ST395, ST42, ST14 and ST101 were identified. Phylogenetic analysis showed that sequence types ST14, ST395 and ST147 are closely related to ST101 and all identified sequence types had a common ancestor ST231. Colistin resistance in K. pneumoniae was attributed to mutations in PhoPQ and PmrAB TCS, while location specific distribution of strains indicates clonal transmission. The results of this study will help in formulation of effective infection prevention and antimicrobial development strategies.
Pushpa Kumari Nirwan, Nirupama Chatterjee, Rajesh Panwar, Mridu Dudeja, Namita Jaggi

1903 related Products with: Mutations in two component system (PhoPQ and PmrAB) in colistin resistant Klebsiella pneumoniae from North Indian tertiary care hospital.

100 ul100.00 ug100 ul50 ul50 ul100.00 ug

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#33760082   // To Up

Activity of cefepime/zidebactam (WCK 5222) against 'problem' antibiotic-resistant Gram-negative bacteria sent to a national reference laboratory.

Triple-action diazabicyclooctanes, e.g. zidebactam, combine β-lactamase inhibition, antibacterial activity, and 'enhancement' of PBP3-targeted β-lactams.
Shazad Mushtaq, Paolo Garello, Anna Vickers, Neil Woodford, David M Livermore

2280 related Products with: Activity of cefepime/zidebactam (WCK 5222) against 'problem' antibiotic-resistant Gram-negative bacteria sent to a national reference laboratory.

1 mL1 KIT100 11 kit1 mL1 mg1 kit1 mg

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#33735537   2021/03/18 To Up

Genetic characterization of Blastocystis from poultry, livestock animals and humans in the southwest region of Iran-Zoonotic implications.

Blastocystis is the most common intestinal parasite found in epidemiological studies in Iran. The genus Blastocystis comprises multiple subtypes (ST), evincing the existence of extensive intrageneric diversity. Meanwhile, information on Blastocystis STs in domestic animals in Iran is limited. This study was designed to identify the prevalence and subtype distribution of Blastocystis in domestic animals and to evaluate the extent of zoonotic origin of human Blastocystis carriage. A total of 395 faecal samples from farm animals (220 from poultry, 100 from sheep and 75 from cattle) and 23 Blastocystis positive samples from humans (available from the previous report of this study and included for reference) from the Khuzestan province, southwestern Iran, were analysed. Standard parasitological methods and PCR-based analysis of the SSU-rRNA gene were used. Overall, 115 (29.1%; 95% CI, 26.5%-31.6%) of the animals were positive for Blastocystis sp. by parasitological methods. The highest colonization rate was found in cattle (50.6%, 38/75), followed by sheep (32.0%, 32/100) and poultry (20.4%, 45/220). Eight Blastocystis STs were identified among 55 sequenced isolates from animals (n = 32) and humans (n = 23): ST3 (27.3%), ST14 (25.4%), ST1 (12.7%), ST7 (12.7%), ST2 (9.1%), ST6 (7.3%), ST5 (3.6%) and ST10 (1.8%). Blastocystis ST1 and ST3 were found in both human and non-human hosts, and examples of shared alleles (strains) were observed. Meanwhile, ST2 was observed only in humans, ST5 only in sheep, ST6 only in poultry and ST10 in only cattle. This is the most comprehensive report of Blastocystis STs in farm animals in Iran.
Roya Salehi, Ali Rostami, Hamed Mirjalali, Christen Rune Stensvold, Ali Haghighi

2823 related Products with: Genetic characterization of Blastocystis from poultry, livestock animals and humans in the southwest region of Iran-Zoonotic implications.

100 μg100 μg1 mg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg

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#33720791   2021/03/15 To Up

Distinctive Mobile Genetic Elements Observed in the Clonal Expansion of Carbapenem-Resistant in India.

(Kp), a common multidrug-resistant pathogen, causes a wide spectrum of nosocomial infections with high rates of morbidity and mortality. The emergence of pan drug-resistant international high-risk clones such as ST258, ST14, ST15, ST147, and ST101 is a global concern. This study was performed to investigate the carbapenemases, the plasmid profile, and the clonal relationship among Indian . A total of 290 isolates from seven centers in India were characterized to determine sequence types (STs) and carbapenemases. A subset of isolates was subjected to whole genome sequencing and hybrid genome assembly to obtain the complete genome. Plasmids carrying carbapenemases were characterized to determine the dissemination of carbapenem-resistant (CR) . From this study, 75 different STs were observed with ST231 being predominant. About 79% of the analyzed isolates were CR with 59% ( = 136) producing OXA48-like carbapenemases. While ST231 was the predominant clone among the OXA48-like producers; NDM producers and NDM+OXA48-like producers were mostly associated with ST14. Interestingly, 61% ( = 138) of the total CR were colistin resistant, belonging to 22 different STs. Plasmid profiling shows that was exclusively carried by ColKP3, whereas was associated with IncFII-like plasmids. The highly mosaic genome of coupled with the diverse ecological niches in India makes it a hotspot for antimicrobial resistance, leading to increased morbidity and mortality. Extensive molecular surveillance of the clonal spread of could help in understanding AMR dynamics and thus rework therapeutic management.
Chaitra Shankar, Jobin John Jacob, Suganya Gopal Sugumar, Lavanya Natarajan, Camilla Rodrigues, Purva Mathur, Dip Narayan Mukherjee, Anita Sharma, D S Chitnis, Anudita Bharagava, Abi Manesh, Karthik Gunasekaran, Balaji Veeraraghavan

2986 related Products with: Distinctive Mobile Genetic Elements Observed in the Clonal Expansion of Carbapenem-Resistant in India.

1100 μg

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