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#34525471   2021/09/15 To Up

Multifocal Urinary Tract Metastasis of Colorectal Carcinoma.

Secondary urinary tract tumors are uncommon findings and mainly evolve by direct invasion from adjacent organs. Actual metastatic involvement often develops in the urinary bladder, while the upper urinary tract is infrequently affected. In addition, the lungs, breast, and prostate gland are the usual primary sites. Colorectal carcinoma (CRC) may spread to the ureter directly or seeds via vascular or lymphatic channels. It may pose struggles in the differential diagnosis because CRC shares standard pathologic features with the primary adenocarcinoma of the urinary tract.
Zsuzsanna Fejes, István Előd Király, Ádám Miklós Fehér, Péter György Kovács, Zoltán Gyuris, Farkas Sükösd, László Torday, Levente Kuthi

2927 related Products with: Multifocal Urinary Tract Metastasis of Colorectal Carcinoma.

0.1 mg

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#34525424   2021/09/01 To Up

Identification of vicilin, legumin and antimicrobial peptide 2a as macadamia nut allergens.

Macadamia nut is an increasingly popular food item of a healthy diet. However, macadamia nut is also a potent allergenic food. To date, there is little information about the allergenic proteins involved. In this study, using sera from macadamia nut allergic individuals, four IgE-binding proteins were detected. Their identities were determined by tandem mass spectrometry with de novo sequencing. Three IgE-reactive proteins, the vicilin Mac i 1, the legumin Mac i 2 and the antimicrobial peptide 2a/Mac i 1 (28-76) were purified from the nut while the non-specific lipid transfer protein was produced as a recombinant in Pichia pastoris. IgE-binding assays using sera from well-characterized groups of tree nut and/or peanut allergic patients revealed that the allergens were mainly recognized by sera from macadamia nut allergic individuals. Hence, these newly discovered allergens will enable molecular diagnostics to identify patients at high risk of macadamia nut allergy.
Stefan Kabasser, Kunal Pratap, Sandip Kamath, Aya C Taki, Thanh Dang, Jennifer Koplin, Kirsten Perrett, Karin Hummel, Christian Radauer, Heimo Breiteneder, Andreas L Lopata, Merima Bublin

2734 related Products with: Identification of vicilin, legumin and antimicrobial peptide 2a as macadamia nut allergens.

50 ug96 assays50 50 ug50 ug50 ug1 ml100 μl100ug10000 tests1 kit(96 Wells)

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#34525401   2021/09/12 To Up

Longitudinal Genomic Characterization of Carbapenemase-producing Enterobacteriaceae (CPE) Reveals Changing Pattern of CPEs Isolated in Hong Kong Hospitals.

A rising trend of carbapenemase-producing Enterobacteriaceae (CPE) isolated from patients was observed after implementing an active CPE surveillance programme in Hong Kong hospitals from 2011. We described the molecular epidemiology of 567 CPE from patients of three of seven public hospital clusters in Hong Kong. During a 7-year period, the incidence of CPE isolation increased from 0.05 to 9.6 /100,000 patient-days. The carbapenemase genes identified were polyclonal, including bla, bla and bla which were mainly associated with hospitalization overseas in earlier years. However, increasing CPE isolation from patients without overseas hospitalization occurred in 2015, with bla (55.1%) becoming predominant followed by and bla (30.0%). E. coli (46.4%) and Klebsiella spp. (38.3%) were the dominant species. Whole genome sequencing was performed on 169 representative isolates with a combination of short and long reads using Illumina and Nanopore technology. Two distinct lineages of bla-positive Klebsiella pneumoniae (ST11&ST258) were identified with ST11 carrying yersiniabactin gene ybt-9 on ICEKp3. ST131 E. coli producing IMP-4 was present throughout the study period. The bla and bla genes were mainly carried in IncX3 and IncN-ST7 plasmids respectively. bla gene was carried in IncX3 plasmid in E. coli but ColKP3 plasmid in K. pneumoniae. A lineage of K. pneumoniae with bla plus bla in distinct plasmids of IncF1B/IncHI1B was identified and associated with prior overseas hospitalization. This study highlights the threat of multiple types of CPE present, with the predominance of bla and bla among CPE in our hospitals. Enhanced containment strategies are needed to mitigate the rapidly rising trends of CPE in healthcare settings.
Chendi Zhu, Carmen Li, Christopher K C Lai, Rita Ng, Sky Chau, Kam-Tak Wong, Norman W S Lo, Nilakshi Barua, Ying Yang, Veranja Liyanapathirana, Mamie Hui, Raymond W Lai, Kitty S C Fung, Dominic N Tsang, Margaret Ip

2165 related Products with: Longitudinal Genomic Characterization of Carbapenemase-producing Enterobacteriaceae (CPE) Reveals Changing Pattern of CPEs Isolated in Hong Kong Hospitals.

21 mL1001050 ul300 units100 ug 5 G100 ul100 ul50 ulSize: 1 mL 100 mM x 2

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#34525398   2021/09/12 To Up

Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.

Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
Mohammad R Hasan, Mahesh K R Kalikiri, Faheem Mirza, Sathyavathi Sundararaju, Anju Sharma, Thabisile Xaba, Stephan Lorenz, Hiam Chemaitelly, Reham A El-Kahlout, Kin Ming Tsui, Hadi M Yassine, Peter V Coyle, Abdullatif Al Khal, Roberto Bertollini, Mohamed H Al Thani, Laith J Abu-Raddad, Patrick Tang,

2752 related Products with: Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.

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#34525378   2021/09/01 To Up

Antihypertensive drug treatment and susceptibility to SARS-CoV-2 infection in human PSC-derived cardiomyocytes and primary endothelial cells.

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Jessika Iwanski, Sobhi G Kazmouz, Shuaizhi Li, Ben Stansfield, Tori T Salem, Samantha Perez-Miller, Toshinobu Kazui, Lipsa Jena, Jennifer L Uhrlaub, Scott Lick, Janko Nikolich-Žugich, John P Konhilas, Carol C Gregorio, May Khanna, Samuel K Campos, Jared M Churko

2856 related Products with: Antihypertensive drug treatment and susceptibility to SARS-CoV-2 infection in human PSC-derived cardiomyocytes and primary endothelial cells.

1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask100ug Lyophilized1.00 flask100ug Lyophilized1.00 flask1.00 flask

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#34525376   // To Up

Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo.

During embryogenesis, waves of hematopoietic progenitors develop from hemogenic endothelium (HE) prior to the emergence of self-renewing hematopoietic stem cells (HSCs). Although previous studies have shown that yolk-sac-derived erythromyeloid progenitors and HSCs emerge from distinct populations of HE, it remains unknown whether the earliest lymphoid-competent progenitors, multipotent progenitors, and HSCs originate from common HE. In this study, we demonstrate by clonal assays and single-cell transcriptomics that rare HE with functional HSC potential in the early murine embryo are distinct from more abundant HE with multilineage hematopoietic potential that fail to generate HSCs. Specifically, HSC-competent HE are characterized by expression of CXCR4 surface marker and by higher expression of genes tied to arterial programs regulating HSC dormancy and self-renewal. Taken together, these findings suggest a revised model of developmental hematopoiesis in which the initial populations of multipotent progenitors and HSCs arise independently from HE with distinct phenotypic and transcriptional properties.
Tessa Dignum, Barbara Varnum-Finney, Sanjay R Srivatsan, Stacey Dozono, Olivia Waltner, Adam M Heck, Takashi Ishida, Cynthia Nourigat-McKay, Dana L Jackson, Shahin Rafii, Cole Trapnell, Irwin D Bernstein, Brandon Hadland

2398 related Products with: Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo.

2 ml10 ug1 x 10^6 cells/vial1 mg100 μg100ug Lyophilized10ml10 100 μg100 μg1 mg100ug Lyophilized

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#34525349   // To Up

Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development.

Human hematopoiesis is a dynamic process that starts in utero 18-21 days post-conception. Understanding the site- and stage-specific variation in hematopoiesis is important if we are to understand the origin of hematological disorders, many of which occur at specific points in the human lifespan. To unravel how the hematopoietic stem/progenitor cell (HSPC) compartment changes during human ontogeny and the underlying gene regulatory mechanisms, we compare 57,489 HSPCs from 5 different tissues spanning 4 developmental stages through the human lifetime. Single-cell transcriptomic analysis identifies significant site- and developmental stage-specific transitions in cellular architecture and gene regulatory networks. Hematopoietic stem cells show progression from cycling to quiescence and increased inflammatory signaling during ontogeny. We demonstrate the utility of this dataset for understanding aberrant hematopoiesis through comparison to two cancers that present at distinct time points in postnatal life-juvenile myelomonocytic leukemia, a childhood cancer, and myelofibrosis, which classically presents in older adults.
Anindita Roy, Guanlin Wang, Deena Iskander, Sorcha O'Byrne, Natalina Elliott, Jennifer O'Sullivan, Gemma Buck, Elisabeth F Heuston, Wei Xiong Wen, Alba Rodriguez Meira, Peng Hua, Anastasios Karadimitris, Adam J Mead, David M Bodine, Irene Roberts, Bethan Psaila, Supat Thongjuea

2033 related Products with: Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development.

1 mg10 ug1.00 flask300 units5ug1.00 flask1.00 flask1.00 flask100 100 μg

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#34525348   2021/09/08 To Up

Visualization of individual cell division history in complex tissues using iCOUNT.

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Annina Denoth-Lippuner, Baptiste N Jaeger, Tong Liang, Lars N Royall, Stefanie E Chie, Kilian Buthey, Diana Machado, Vladislav I Korobeynyk, Merit Kruse, Clara M Munz, Alexander Gerbaulet, Benjamin D Simons, Sebastian Jessberger

1090 related Products with: Visualization of individual cell division history in complex tissues using iCOUNT.

1.00 flask100ug Lyophilized96 samples50 ul100 μg1x10e7 cells

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#34525340   // To Up

Resident memory CD8 T cells in regional lymph nodes mediate immunity to metastatic melanoma.

The nature of the anti-tumor immune response changes as primary tumors progress and metastasize. We investigated the role of resident memory (Trm) and circulating memory (Tcirm) cells in anti-tumor responses at metastatic locations using a mouse model of melanoma-associated vitiligo. We found that the transcriptional characteristics of tumor-specific CD8 T cells were defined by the tissue of occupancy. Parabiosis revealed that tumor-specific Trm and Tcirm compartments persisted throughout visceral organs, but Trm cells dominated lymph nodes (LNs). Single-cell RNA-sequencing profiles of Trm cells in LN and skin were distinct, and T cell clonotypes that occupied both tissues were overwhelmingly maintained as Trm in LNs. Whereas Tcirm cells prevented melanoma growth in the lungs, Trm afforded long-lived protection against melanoma seeding in LNs. Expanded Trm populations were also present in melanoma-involved LNs from patients, and their transcriptional signature predicted better survival. Thus, tumor-specific Trm cells persist in LNs, restricting metastatic cancer.
Aleksey K Molodtsov, Nikhil Khatwani, Jennifer L Vella, Kathryn A Lewis, Yanding Zhao, Jichang Han, Delaney E Sullivan, Tyler G Searles, Nicholas K Preiss, Tamer B Shabaneh, Peisheng Zhang, Aaron R Hawkes, Brian T Malik, Fred W Kolling, Edward J Usherwood, Sandra L Wong, Joseph D Phillips, Keisuke Shirai, Christina V Angeles, Shaofeng Yan, Tyler J Curiel, Yina H Huang, Chao Cheng, Mary Jo Turk

2284 related Products with: Resident memory CD8 T cells in regional lymph nodes mediate immunity to metastatic melanoma.

96 tests

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