Product name : MethylFlash Methylated DNA Quantification Kit (Colorimetric) (48 assays) 

Catalog Number :P-1034-48

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Price :458.85 €

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Supplier :EpigenTek

Product Overview

The MethylFlash™ Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features
The MethylFlash™ Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

  • Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
  • Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
  • High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA from just 50 ng of input genomic DNA.
  • Optimized antibody and enhancer solutions allow high specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
  • Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay flexible: manual or high throughput analysis.


Fig. 1. Schematic procedure for the MethylFlash™ Methylated DNA Quantification Kit (Colorimetric).

Product Details


Fig. 2. Demonstration of high sensitivity and specificity of methylated DNA detection achieved by the MethylFlash™ kit. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with the MethylFlash™ Methylated DNA Quantification Kit (Colorimetric).


 

Principle & Procedure
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit's included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5-mC) using a standard curve.

Easy, Fast, and Flexible
The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

Safe and Convenient
All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion/denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

Product Components

ME1 (10X Wash Buffer)
ME2 (Binding Solution)
ME3 (Negative Control, 20 µg/ml)*
ME4 (Positive Control, 20 µg/ml)*
ME5 (Capture Antibody, 1000 µg/ml*
ME6 (Detection Antibody, 400 µg/ml)*
ME7 (Enhancer Solution)*
ME8 (Developer Solution)
ME9 (Stop Solution)
8-Well Assay Strips (With Frame)
User Guide

* Spin the solution down to the bottom prior to use.

Note: The ME3 Negative Control is an unmethylated polynucleotide containing 50% of cytosine. The ME4 Positive Control is a methylated polynucleotide containing 50% of 5-methylcytosine.

Product Citations

Ortiz, R. et. al. Global DNA Methylation in Peripheral Blood mononuclear cells predicts prolonged elevations of plasma Il-6 in Women throughout the Breast Cancer Trajectory. Abstract

Hsu, A. et. al (October 2011). Promoter de-methylation of Cyclin D2 by sulforaphane in prostate cancer cells. Clinical Epigenetics. 3:3. Full Text Article

Ward, A. et. al. (February 2011). Aberrant methylation of Polo-like kinase CpG islands in Plk4 heterozygous mice. BMC Cancer. 11:71. Full PDF Article

Li, W. et. al. (2011). Distribution of 5-hydroxymethylcytosine in different human tissues. J Nucleic Acids. 2011:870726. PubMed Abstract

 

datasheet of product : MethylFlash Methylated DNA Quantification Kit (Colorimetric) (48 assays)   MethylFlash Methylated DNA Quantification Kit (Colorimetric) (48 assays) 

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4257-010-NC Neutral CometAssay Control Cells Trevigen 10 assays 177.00€ Pub
K116-100 Caspase 2 Fluorometric Assay Kit Biovision 100 assays 342.50€
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Pub
K270-50 FractionPREPTM Cell Fractionation Kit Biovision 50 assays 533.93€ Pub
K780-100 DPP4 Inhibitor Screening Kit Biovision 100 assays 503.00€
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Pub
1113-200 Caspase-10 Substrate AEVD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
K028-H1 Superoxide Dismutase (SOD) Colorimetric Activity kit (2 Plates) Arbor Assays 2 x 96 well plate 337.00€ Pub
K201-100 Annexin V FITC Apoptosis Kit Plus Biovision 100 assays 333.00€
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Pub
K652-100 Alanine Assay Kit Biovision 100 assays 357.00€ Pub
BAH67-1000 Protease, DNASE free heat shock BSA powder, 1KG Equitech 1KG 772.80€
ASK
Pub
1075-200 Caspase-9 Substrate LEHD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
bs-1276R-Cy3 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Cy3 Conjugated Bioss 100ug 369.00€ Pub
K151-100 Caspase 1 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
ASK
Pub
K510-100 Nickel Assay Kit Biovision 100 assays 367.00€ Pub
bs-1027R-FITC Rabbit Anti-NSE ENO2 Polyclonal Antibody, FITC Conjugated, 48kDa; Isotype IgG; Reactivity Human , Mouse , Rat , Dog , Bovine , Chicken; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
1013-200 Annexin V Cy5 Reagent200 assays Biovision 200 assays 325.08€
ASK
Pub
BT10E 10 µl Eppendorf Style Barrier Tip Filtered Sterile 10 Neptune 4800 (in case - Racked - 96) 383.00€
ASK
Pub
K120-50 Quick Apoptotic DNA Ladder Detection Kit Biovision 50 assays 338.00€
ASK
Pub
MD-14-0255P Recombinant Human SDF-1a KITLG Proteins Ray Biotech 10 309.00€
ASK
Pub
1015-1000 Annexin V PE Cy5 Reagent Biovision 1000 assays 945.50€
ASK
Pub
BT200 200 µl Barrier Tip Filtered Sterile 10 Neptune 4800 (in case - Racked - 96) 383.00€ Pub
K335-100 SOD Activity Activity Assay Kit Biovision 100 assays 299.00€ Pub
228-11411-1 Recombinant Rat SCF KITLG Proteins Ray Biotech 2 147.00€ Pub
P-3015-096 EpiQuik In Situ Histone H3K4 Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
11305 Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit AAT Bioquest 1 kit 202.00€
ASK
Pub
K109-100 Annexin V Biotin Apoptosis Kit Biovision 100 assays 333.50€
ASK
Pub
K256-25 Mitochondria/Cytosol Fractionation Kit Biovision 25 assays 187.11€
ASK
Pub
K711-100 Amylase Activity Assay Kit Biovision 100 assays 269.00€ Pub
LF-PA0057 anti-CAD (caspase-activated Dnase, DFF40) , Rabbit polyclonal to CAD (caspase-activated Dnase, DFF40) , Isotype IgG, Host Rabbit Abfrontier 100 ul 355.00€ Pub
1102-200 Caspase 5 Substrate WEHD pNA200 assays Biovision 200 assays 287.28€
ASK
Pub
228-10332-1 Recombinant Human HSP70 DnaK Proteins Ray Biotech 10 147.00€
ASK
Pub
K182-25 CaspGLOW Fluorescein Active Caspase 2 Staining Kit Biovision 25 assays 200.00€
ASK
Pub
CQMT-500 CellQuanti MTT™ Cell Viability Assay Kits Bioassays 500 174.79€ Pub
1069-1000 Caspase-6 Substrate VEID-AFC; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
EDLC-100 EnzyChrom™ D-Lactate Assay Kit, Quantitative determination of D-lactic acid by colorimetric (565nm) method. Procedure 20 min. Kit size 100 tests. Detection limit 0.05 mM. Shelf life 6 months. Ship Bioassays 100tests 345.00€ Pub
K377-100 Acetyl CoA Assay Kit 100 assays[DISCONTINUED] Biovision 100 assays 325.77€
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Pub
M12806621 Carbenicillin disodium salt CAS Number [4800 94 6] Molekula 5 G 197.00€
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Pub
Z5030005 QBlue Cell Viability Assay Kit Biochain 10,000 assays 498.00€
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Pub
1002-1000 Annexin V Cy3 Reagent1000 assays Biovision 1000 assays 891.00€
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Pub
291-59201 GLP 1 ELISA Kit, Rat Glucagon like peptide 1 (GLP 1) is an incretin hormone, which is synthesized in intestinal endocrine cells. This peptide is known to increase insulin secretion by glucose stimulat B-Bridge 96 assays 1 040.55€
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Pub
K266-25 Nuclear/Cytosol Fractionation Kit Biovision 25 assays 187.11€
ASK
Pub
K757-100 Glucose 6 Phosphate Dehydrogenase Assay Kit Biovision 100 assays 357.73€ Pub
P-1015-24  Methylamp Methylated DNA Capture (MeDIP) Kit (24 reactions) EpigenTek 448.00€
ASK
Pub
1109-200 Caspase-4 Substrate LEVD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
K190-25 CaspGLOW Red Active Caspase Staining Kit Biovision 25 assays 200.00€
ASK
Pub
228-10336-1 Native Bacterial DNase Proteins Ray Biotech 20mg 147.00€
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Pub
1071-200 Caspase 2 Substrate VDVAD AFC Biovision 200 assays 311.85€
ASK
Pub
DS-MB-03301 Mouse Anti-Human HSP40 DnaJ Antibodies Ray Biotech 0.1 mg 452.00€
ASK
Pub
K421-500 Acid Phosphatase Fluorometric Assay Kit Biovision 500 assays 250.00€ Pub
M54436559 Sitagliptin CAS: [486460-32-6] Molekula 1 g 399.00€
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Pub
1007-200 Caspase 3 Substrate DEVD AFC Biovision 200 assays 311.85€
ASK
Pub
4822-96-K TiterTACS Colorimetric Kit Trevigen 96 Samples 401.00€
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Pub
K786-100 Glucose Dehydrogenase Activity Assay Kit Biovision 100 assays 367.00€ Pub
1114-1000 Caspase 10 Substrate AEVD pNA1000 assays Biovision 1000 assays 579.29€
ASK
Pub
K101-100 Annexin V FITC Apoptosis Kit Biovision 100 assays 333.00€ Pub
K202-400 Annexin V Cy3 Apoptosis Kit Plus Biovision 400 assays 666.50€
ASK
Pub
K659-100 Oxaloacetate Assay Kit Biovision 100 assays 357.00€ Pub
bs-7653R-A647 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323.00€ Pub
1076-1000 Caspase 9 Substrate LEHD pNA1000 assays Biovision 1000 assays 579.29€
ASK
Pub
bs-1276R-HRP Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, HRP Conjugated Bioss 100ug 369.00€ Pub
K156-100 Caspase 6 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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Pub
K566-100 Sialic Acid (NANA) Assay Kit Biovision 100 assays 435.00€ Pub
bs-7653R-Cy7 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy7 conjugated Isotype: IgG Sepax 100ug Lyophilized 268.00€ Pub
1101-1000 Caspase-5 Substrate WEHD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
bs-1276R-PE-Cy7 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy7 Conjugated Bioss 100ug 401.00€ Pub
K163-100 CaspSELECT Caspase 3 Immunoassay Kit Biovision 100 assays 649.00€
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Pub
K603-100 Cholesterol Cholesteryl Ester Quantitation Kit Biovision 100 assays 377.00€ Pub
BQ007-EAEL ENZYMATIC ASSAY KITS (CHEMISTRY AUTOANALYZER), Electrolytes: CO2 (405nm) Assay Kit (Enzymatic, liquid stable) Bioquant 300 tests 319.00€ Pub
1062-1000 Caspase 8 Substrate IETD AFC Biovision 1000 assays 613.31€
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Pub
D2NO-100 QuantiChrom™ Nitric Oxide Assay Kit, Quantitative determination of nitric oxide by colorimetric (540nm) method Bioassays 100Tests 265.00€ Pub
K124-200 Caspase 10 Fluorometric Assay Kit Biovision 200 assays 495.50€ Pub
K347-100 NADP NADPH Quantitation Kit Biovision 100 assays 435.00€ Pub
228-11412-3 Recombinant Human SCF KITLG Proteins Ray Biotech 1mg 2 426.00€ Pub
P-4008-48 EpiQuik Global Histone H3 Acetylation Assay Kit 48 assays EpigenTek 48 assays 490.35€
ASK
Pub
13620 Amplite™ Colorimetric Sphingomyelinase Assay Kit *Blue Color* AAT Bioquest 1 kit 202.00€
ASK
Pub
K110-25 Caspase 1 Fluorometric Assay Kit Biovision 25 assays 162.50€
ASK
Pub
K724-100 Lipase Activity Assay Kit III Biovision 100 assays 425.00€ Pub
P-1012-2 FitAmp Circulating DNA Quantification Kit EpigenTek 96 samples 388.50€
ASK
Pub
1103-200 Caspase 1 Substrate YVAD AFC Biovision 200 assays 311.85€
ASK
Pub
228-10333-3 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 1mg 1 031.00€
ASK
Pub
K188-100 Caspase 8 Staining Kit, CaspGLOW Green Biovision 100 assays 400.00€
ASK
Pub
K620-100 Ethanol Assay Kit Biovision 100 assays 406.00€ Pub
OP-0002-1  EpiQuik Nuclear Extraction Kit (100 assays) EpigenTek 239.40€ Pub
1069-200 Caspase 6 Substrate VEID AFC200 assays Biovision 200 assays 276.89€
ASK
Pub
EPPD-100 EnzyChrom™ Phospholipase D Assay Kit, Quantitative determination of phospholipase D activity by colorimetric (570nm) or fluorimetric (530 590nm) method. Procedure 30 min. Kit size 100 tests. Detecti Bioassays 100tests 287.00€ Pub
K128-25 Annexin V PE Apoptosis Detection Kit Biovision 25 assays 171.50€
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Pub
K401-60 ApoBrdU DNA Fragmentation Assay Kit Biovision 60 assays 533.00€
ASK
Pub
M56283827 2 Isopropoxyphenol CAS Number [4812 20 8] Molekula 100 G 135.00€
ASK
Pub
  OP-0004-2  CytoX Red Cell Proliferation Cytotoxicity Assay Kit (5x96 assays) EpigenTek 214.00€
ASK
Pub
1004-1000 Annexin V EGFP Reagent Biovision 1000 assays 945.50€
ASK
Pub
36350 Screen Quest™ Colorimetric Chloride Channel Assay Kit AAT Bioquest 10 Plates 635.00€
ASK
Pub
K114-25 Caspase 6 Fluorometric Assay Kit Biovision 25 assays 162.50€
ASK
Pub
K763-100 Thioredoxin Reductase Assay Kit Biovision 100 assays 357.00€ Pub
1110-1000 Caspase 4 Substrate LEVD pNA1000 assays Biovision 1000 assays 579.29€
ASK
Pub
HBM-RKIT-W03-100 EXOTESTTM ready to use kit for exosome capture and quantification. White plate for luminometric readings with anti CD81 primary antibody. HansaBioMed 96 T 1 158.02€
ASK
Pub
K198-100 CaspGLOW Red Active Caspase 8 Staining Kit Biovision 100 assays 482.00€
ASK
Pub
K637-100 Malate Assay Kit Biovision 100 assays 357.00€ Pub
228-10337-3 Recombinant Human DNase Proteins Ray Biotech 10,000IU 601.00€
ASK
Pub
1072-200 Caspase-2 Substrate VDVAD-pNA; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
bs-1276R Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Unconjugated Bioss 100ug 330.00€ Pub
K140-100 Cathepsin B Activity Assay Kit Biovision 100 assays 357.00€
ASK
Pub
K470-100 Ammonia Assay Kit II Biovision 100 assays 386.00€ Pub
B286845 5-(Benzyloxy)-2-(methylthio)pyrimidine C12H12N2OS CAS: 4874-32-2 TRC 25 mg 232.00€
ASK
Pub
1008-200 Caspase-3 Substrate DEVD-pNA; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
AK8088-1000 Cell Proliferation Kit XTT Akron 1000 assays 228.00€ Pub
K118-25 Caspase 9 Fluorometric Assay Kit Biovision 25 assays 171.50€
ASK
Pub
K302-500 Quick Cell Proliferation Assay Kit Plus Biovision 500 assays 230.00€ Pub
K791-100 StayBrite™ Highly Stable ATP Assay Kit Biovision 100 assays 191.00€ Pub
1116-1000 Caspase 13 Substrate LEED AFC Biovision 1000 assays 613.31€
ASK
Pub
K104-100 Annexin V EGFP Apoptosis Kit Biovision 100 assays 333.00€
ASK
Pub
K245-100 Proteasome Activity Assay Kit Biovision 100 assays 367.00€ Pub
K672-100 Heme Assay Kit Biovision 100 assays 357.00€ Pub
1117-1000 Caspase-12 Substrate ATAD-AFC; Appearance Liquid Biovision 1000 assays 1 039.50€
ASK
Pub
K105-25 Caspase 3 Fluorometric Assay Kit Biovision 25 assays 162.50€
ASK
Pub
K254-1000 ATP Cell Viability Assay Kit, ApoSENSOR Biovision 1000 assays 667.00€
ASK
Pub
K705-400 PhosphoSeek™ PDE5A Assay Kit Biovision 400 Assays 669.00€ Pub
bs-7653R-PE-Cy3 Rabbit Anti-DNase gamma Polyclonal Antibody, PE-Cy3 Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 530.00€ Pub
1101-200 Caspase 5 Substrate WEHD AFC200 assays Biovision 200 assays 276.89€
ASK
Pub
228-10330-1 Recombinant E. coli HSP70 DnaK Proteins Ray Biotech 10 147.00€ Pub
K172-25 CaspGLOW Fluorescein Active Caspase 12 Staining Kit Biovision 25 assays 226.00€
ASK
Pub
K608-100 Uric Acid Assay Kit Biovision 100 assays 386.00€ Pub
CQBL-05K CellQuanti Blue™ Cell Viability Assay Kits Bioassays 5000 170.89€ Pub
1063-1000 Caspase-8 Substrate IETD-pNA; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
E2HL-100 EnzyChrom™ AF HDL and LDL VLDL Assay Kit, Quantitative determination of HDL and LDL VLDL cholesterol by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 60 min. Kit size 100 tes Bioassays 100tests 393.00€ Pub
K363-100 HDAC-3 Inhibitor Screening Kit Biovision 100 assays 503.00€
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Pub
K810-1000 Protein Quantitation Kit Biovision 1000 assays 240.00€ Pub
PPO-200 ProPrep™ Omni 200 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group Up to 200 ml cultures 460.00€ Pub
21659 PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV absorption* AAT Bioquest 1 kit 202.00€
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Pub
K745-100 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit Biovision 100 assays 406.00€ Pub
1104-200 Caspase-1 Substrate YVAD-pNA; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
228-10335-2 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 100 205.00€
ASK
Pub
K189-100 CaspGLOW Fluorescein Active Caspase 9 Staining Kit Biovision 100 assays 482.00€
ASK
Pub
K625-100 Creatinine Assay Kit Biovision 100 assays 308.00€ Pub
bs-1359R Rabbit Anti-DNAPK PRKDC Polyclonal Antibody, Unconjugated Bioss 100ug 330.00€ Pub
1070-200 Caspase 6 Substrate VEID pNA Biovision 200 assays 311.85€
ASK
Pub
228-10327-1 Recombinant E. coli HSP40 DnaJ Proteins Ray Biotech 20 147.00€ Pub
K130-50 Enhanced Apoptotic DNA Ladder Detection Kit Biovision 50 assays 338.00€
ASK
Pub
M59401089 2 Methyl 3 nitroanisole (2 Methoxy 6 nitrotoluene) CAS Number [4837 88 1] Molekula 1 G 104.00€
ASK
Pub
  P-4004-192  EpiQuik In Situ H3K9 Acetylation Assay Kit (2x96 assays) EpigenTek 841.00€
ASK
Pub
10053 Amplite™ Colorimetric Aldehyde Quantitation Kit *Blue Color* AAT Bioquest 1 kit 280.00€
ASK
Pub
4256-010-CC CometAssay Control Cells Trevigen 10 assays 177.00€ Pub
K779-100 DPP4 Activity Assay Kit Biovision 100 assays 386.00€
ASK
Pub
1113-1000 Caspase 10 Substrate AEVD AFC1000 assays Biovision 1000 assays 541.49€
ASK
Pub
K013-H1 Hemoglobin Colorimetric Detection kit (2 plate) Arbor Assays 2x96 well plates 248.00€
ASK
Pub
K200-25 CaspSCREEN Caspase Screening Kit Biovision 25 assays 264.00€
ASK
Pub
K647-100 Starch Assay Kit Biovision 100 assays 357.00€ Pub
BAH67-0500 Protease, DNASE free heat shock BSA powder, 500gm Equitech 500gm 557.55€
ASK
Pub
1075-1000 Caspase 9 Substrate LEHD AFC1000 assays Biovision 1000 assays 541.49€
ASK
Pub
bs-1276R-Biotin Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Biotin Conjugated Bioss 100ug 369.00€ Pub
K145-100 Cathepsin H Activity Assay Kit Biovision 100 assays 357.00€
ASK
Pub
K505-100 Cobalt Assay Kit Biovision 100 assays 357.00€ Pub
bs-0489R-FITC Rabbit Anti-BrdU Polyclonal Antibody, FITC Conjugated, 20 48kDa; Isotype IgG; Reactivity Others; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
1013-200 Annexin V Cy5 Reagent Biovision 200 assays 315.50€
ASK
Pub
BT100 100 µl Barrier Tip, Filtered, Sterile, Low Retention, Racked 96 Biotix 4800 248.00€ Pub
K313-500 LDH Cytotoxicity Assay Kit II Biovision 500 assays 270.50€
ASK
Pub
K802-250 Beta Galactosidase Staining Kit Biovision 200 assays. 207.50€ Pub
P-3002-2 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K4 EpigenTek 96 assays 769.65€ Pub
1014-200 Annexin V PE Reagent200 assays Biovision 200 assays 325.00€
ASK
Pub
BT20 20 µl Barrier Tip Filtered Sterile 10 Neptune 4800 (in case - Racked - 96) 383.00€ Pub
K122-400 Caspase 5 Fluorometric Assay Kit Biovision 400 assays 738.50€
ASK
Pub
K332-100 HAT Activity Activity Assay Kit Biovision 100 assays 396.00€
ASK
Pub
228-11410-3 Recombinant Mouse SCF KITLG Proteins Ray Biotech 1mg 2 426.00€
ASK
Pub
P-3014T-096 EpiQuik In Situ Histone H3K27 Tri Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
1117-200 Caspase 12 Substrate ATAD AFC200 assays Biovision 200 assays 381.78€
ASK
Pub
K710-100 Xanthine Oxidase Activity Assay Kit Biovision 100 assays 289.00€ Pub
G028 DNase I ABM Goods 1ml / 2000U 71.40€ Pub
1102-200 Caspase 5 Substrate WEHD pNA Biovision 200 assays 311.85€
ASK
Pub
228-10331-3 Recombinant E. coli HSP70 DnaK ATPase BD Proteins Ray Biotech 1mg 1 658.00€
ASK
Pub
K182-100 CaspGLOW Fluorescein Active Caspase 2 Staining Kit Biovision 100 assays 482.00€
ASK
Pub
CQMT-500 CellQuanti-MTT™ Cell Viability Assay Kits Bioassays 500 tests 133.00€ Pub
1063-200 Caspase 8 Substrate IETD pNA200 assays Biovision 200 assays 276.89€
ASK
Pub
EDLC-100 EnzyChrom™ D-Lactate Assay Kit, Quantitative determination of D-lactic acid by colorimetric (565nm) method Bioassays 100Tests 362.00€ Pub
K375-100 Urea Assay Kit Biovision 100 assays 377.00€ Pub
M1224 Rhodamine B octadecyl ester perchlorate, Lipophilic energy transfer acceptor from lipophilic fluoresceins in fluorescence energy transfer (FRET) assays in cell fusion experiments, 10mg MarkerGene 168.00€
ASK
Pub
STA-330 OxiSelect™ TBARS Assay Kit (MDA Quantitation) Cell Biolabs 200 assays 489.00€ Pub
1002-1000 Annexin V Cy3 Reagent Biovision 1000 assays 945.50€
ASK
Pub
291-59201 GLP 1 ELISA Kit, Rat B-Bridge 96 assays 1 390.75€
ASK
Pub
K757-100 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit Biovision 100 assays 357.00€ Pub
P-1015-24 Methylamp Methylated DNA Capture (MeDIP) Kit (24 reactions) EpigenTek 440.00€
ASK
Pub
1109-1000 Caspase 4 Substrate LEVD AFC1000 assays Biovision 1000 assays 541.49€
ASK
Pub
HBM-PPT01-100/5 EXOTESTTM precoated plate for exosome capture. Set of 5. Transparent for colorimetric readings. HansaBioMed 96 T 1 115.31€
ASK
Pub
K190-25 Caspase Staining Kit, CaspGLOW Red Biovision 25 assays 165.00€
ASK
Pub
K630-100 Free Glycerol Assay Kit Biovision 100 assays 377.00€ Pub
22663 DNase I antibody Source Rabbit Polyconal Ab Species Human Application WB IF SignalWay Antibody S.A.B 100ul 329.00€
ASK
Pub
1071-200 Caspase-2 Substrate VDVAD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
228-10328-3 Recombinant Human HSP40 DnaJ Proteins Ray Biotech 1mg 2 426.00€
ASK
Pub
K135-7-25 Caspase Fluorometric Substrate Set Plus Biovision 7 x 25 assays 726.00€ Pub
K420-100 Phosphate Fluorometric Assay Kit Biovision 100 assays 260.00€ Pub
A601860 2-Amino-5-bromopyrazine-3-carboxylic Acid C5H4BrN3O2 CAS: 486424-37-7 TRC 1 g 245.00€
ASK
Pub
1007-200 Caspase-3 Substrate DEVD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
4700-192-K Tankyrase 1 Colorimetric Activity Assay Trevigen 192 samples 1 342.00€
ASK
Pub
K280-50 Mitochondrial DNA Isolation Kit Biovision 50 assays 425.00€ Pub
1114-1000 Caspase 10 Substrate AEVD pNA Biovision 1000 assays 634.10€
ASK
Pub
K039-H1 Glucose Colorimetric Detection Kit (Two Plate) Arbor Assays 2x96 well plate 301.00€ Pub
K202-25 Annexin V Cy3 Apoptosis Kit Plus Biovision 25 assays 180.50€
ASK
Pub
K657-100 Glucose 6 Phosphate Assay Kit Biovision 100 assays 351.50€ Pub
bs-7653R-A555 Rabbit Anti-DNase gamma Polyclonal Antibody, Alexa Fluor 555 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323.00€ Pub
1076-1000 Caspase 9 Substrate LEHD pNA Biovision 1000 assays 634.10€
ASK
Pub
bs-1276R-FITC Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, FITC conjugated,Isotype: IgG Sepax 100ug Lyophilized 304.00€ Pub
K155-100 Caspase 5 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
ASK
Pub
K564-100 Branched Chain Amino Acid (Leu Ile Val) Assay Kit Biovision 100 assays 367.00€ Pub
MD-14-0253P Recombinant Mouse SCF KITLG Proteins Ray Biotech 10 309.00€
ASK
Pub
11001 ReadiUse™ ABTS Solution *Optimized for ELISA Assays with HRP Conjugates AAT Bioquest 1 L 192.00€
ASK
Pub
bs-1276R-PE-Cy5.5 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG Sepax 100ug Lyophilized 300.00€
ASK
Pub
K160-100 Caspase 10 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
ASK
Pub
K602-100 CETP Inhibitor Drug Screening Kit Biovision 100 assays 533.00€
ASK
Pub
BIO-37042 DNA kits, Column-free Isolation: SureClean Bioline 1 x 5ml 62.00€
ASK
Pub
1062-1000 Caspase-8 Substrate IETD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
CSOD100-2 Colorimetric Superoxide Dismutase Celleng-tech 1 kit 322.35€ Pub
K124-100 Caspase 10 Fluorometric Assay Kit Biovision 100 assays 360.50€
ASK
Pub
K340-100 HDAC Inhibitor Drug Screening Kit Biovision 100 assays 435.00€
ASK
Pub
228-11412-2 Recombinant Human SCF KITLG Proteins Ray Biotech 10 205.00€ Pub
P-3021-48  EpiQuik MBD2 Binding Activity Inhibition Assay Kit (48 assays) EpigenTek 474.60€
ASK
Pub
13620 Amplite™ Colorimetric Sphingomyelinase Assay Kit AAT Bioquest 1 kit 212.00€
ASK
Pub
K110-200 Caspase 1 Fluorometric Assay Kit Biovision 200 assays 450.00€
ASK
Pub
K260-100 GST Fluorometric Activity Assay Kit Biovision 100 assays 367.00€
ASK
Pub
K723-100 Lipase Assay Kit II Biovision 100 assays 389.00€ Pub
P-1012-1  FitAmp Circulating DNA Quantification Kit (48 samples) EpigenTek 231.00€
ASK
Pub
1103-200 Caspase-1 Substrate YVAD-AFC; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
228-10333-2 Recombinant E. coli HSP70 DnaK LCS Proteins Ray Biotech 100 205.00€
ASK
Pub
K183-25 CaspGLOW Fluorescein Active Caspase 3 Staining Kit Biovision 25 assays 200.00€
ASK
Pub
K619-100 Fructose Assay Kit Biovision 100 assays 357.00€ Pub
OP-0002-1 EpiQuik Nuclear Extraction Kit EpigenTek 100 assays 239.40€ Pub
1069-200 Caspase 6 Substrate VEID AFC Biovision 200 assays 311.85€
ASK
Pub
ENH3-100 EnzyChrom™ Ammonia Assay Kit, Quantitative determination of ammonia ammonium concentration by colorimetric (340nm) or fluorimetric (360nm 450nm) methods. Bioassays 100Tests 271.00€ Pub
K390-100 Iron Assay Kit Biovision 100 assays 377.00€ Pub
B123505 BAPTA-tetracesium Salt C22H20Cs4N2O10 CAS: 480436-84-8 TRC 1 g 245.00€
ASK
Pub
  OP-0004-1  CytoX Red Cell Proliferation Cytotoxicity Assay Kit (3x96 assays) EpigenTek 174.00€
ASK
Pub
1003-1000 Annexin V Biotin Reagent1 mg (4000 assays) Biovision 1 mg (4000 assays) 2 073.00€
ASK
Pub
32000-1 Live Bacterial Gram Stain Kit 200 assays Biotium 1 KIT 177.45€ Pub
K114-200 Caspase 6 Fluorometric Assay Kit Biovision 200 assays 450.50€
ASK
Pub
K267-50 Cytosol Particulate Separation Kit Biovision 50 assays 308.00€ Pub
K762-100 Glutathione Peroxidase Activity Assay Kit Biovision 100 assays 357.00€ Pub
P-1019-1  Methylamp Universal Methylated DNA Preparation Kit EpigenTek 287.70€
ASK
Pub
1110-1000 Caspase 4 Substrate LEVD pNA Biovision 1000 assays 634.10€
ASK
Pub
HBM-RKIT-W02-100 EXOTESTTM ready to use kit for exosome capture and quantification. White plate for luminometric readings with anti CD9 primary antibody. HansaBioMed 96 T 1 158.02€
ASK
Pub
K198-100 Caspase 8 Staining Kit, CaspGLOW Red Biovision 100 assays 399.00€
ASK
Pub
K636-100 Sarcosine Assay Kit Biovision 100 assays 357.00€ Pub
228-10337-2 Recombinant Human DNase Proteins Ray Biotech 1500IU 205.00€
ASK
Pub
1072-1000 Caspase 2 Substrate VDVAD pNA1000 assays Biovision 1000 assays 579.29€
ASK
Pub
ER-14-0857 Goat Anti-Human MDG1 DNAJB9, (C Terminus) Antibodies Ray Biotech 100 μg 353.00€
ASK
Pub
K139-25 Caspase 12 Fluorometric Assay Kit Biovision 25 assays 180.50€
ASK
Pub
K435-40 KinaseSTAR Akt Activity Assay Kit Biovision 40 assays 687.00€ Pub
A578860 5-(2-Aminoacetamido)-2,4,6-triiodo-isophthalic Acid C10H7I3N2O5 CAS: 4873-46-5 TRC 2.5 mg 245.00€
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1008-1000 Caspase 3 Substrate DEVD pNA1000 assays Biovision 1000 assays 541.00€
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5525 Amplite™ Colorimetric Maleimide Quantitation Kit AAT Bioquest 1 kit 202.00€
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K118-200 Caspase 9 Fluorometric Assay Kit Biovision 200 assays 450.50€
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K302-2500 Quick Cell Proliferation Assay Kit Plus Biovision 2500 assays 435.00€ Pub
K790-10000 StayBrite™ Highly Stable Luciferase Luciferin Reagent Biovision 10000 assays 4 876.00€ Pub
1116-1000 Caspase-13 Substrate LEED-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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K103-100 Annexin V Cy5 Apoptosis Kit Biovision 100 assays 333.00€
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K240-100 Calpain Activity Assay Kit Biovision 100 assays 435.00€
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K671-100 Ascorbic Acid Assay Kit II (FRASC) Biovision 100 assays 406.00€ Pub
bs-7653R-Cy5.5 Rabbit Anti-DNase gamma Polyclonal Antibody, Cy5.5 conjugated, Isotype: IgG Sepax 100ug Lyophilized 268.00€
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1116-200 Caspase 13 Substrate LEED AFC200 assays Biovision 200 assays 276.89€
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K105-200 Caspase 3 Fluorometric Assay Kit Biovision 200 assays 450.50€
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K252-200 Nitric Oxide Fluorometric Assay Kit Biovision 2 x 96 assays 431.00€
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K689-100 PicoProbe™ Fructose-6-Phosphate Assay Kit Biovision 100 assays 425.00€ Pub
bs-7653R-PE Rabbit Anti-DNase gamma Polyclonal Antibody, PE Conjugated , 36kDa; Isotype IgG; Reactivity Human , Mouse , Rat; Application Flow-Cyt(1 20-100), IF(1 50-200) Bioss 100ug Lyophilized 502.00€ Pub
1101-200 Caspase 5 Substrate WEHD AFC Biovision 200 assays 311.85€
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228-10329-3 Recombinant M. tuberculosis HSP70 DnaK Proteins Ray Biotech 100 1 312.00€
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K172-25 Caspase 12 Staining Kit, CaspGLOW Green Biovision 25 assays 186.00€
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K607-100 Lactate Assay Kit Biovision 100 assays 425.00€ Pub
BQ062A-EAKP ENZYMATIC ASSAY KITS (CHEMISTRY AUTOANALYZER), KIDNEY AND PANCREATIC DISEASES: N-Acetyl-p-D-Glucosaminidase (NAG) Kit Bioquant 540 tests 448.00€ Pub
1062-200 Caspase 8 Substrate IETD AFC200 assays Biovision 200 assays 276.89€
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DIUR-500 QuantiChrom™ Urea Assay Kit, Quantitative determination of urea by chemical colorimetric (520nm) method Bioassays 500Tests 300.00€ Pub
K360-100 Beta Secretase Fluorometric Assay Kit Biovision 100 assays 464.00€
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DI0207 KITLG & FLT3LG Protein Protein Interaction Antibody Pair Abnova 1 Set 765.00€
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PLF-960 ProPrep™ BAC 960 High-Throughput BAC & Plasmid DNA Isolation Kits Biotech support group 10x96, 2.0ml cultures 1 501.00€ Pub
15260 Amplite™ Colorimetric NADP NADPH Assay Kit AAT Bioquest 1 kit 202.00€
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K264-100 Glutathione (GSH GSSG Total) Assay Kit Biovision 100 assays 435.00€ Pub
1104-1000 Caspase 1 Substrate YVAD pNA1000 assays Biovision 1000 assays 541.49€
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228-10335-1 Recombinant E. coli HSP70 DnaK SBD Proteins Ray Biotech 20 147.00€
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K189-100 Caspase 9 Staining Kit, CaspGLOW Green Biovision 100 assays 400.00€
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K624-100 Lactose Assay Kit Biovision 100 assays 357.00€ Pub
hAP-0248 Mouse anti human DNAM-1 Anti-Human antibodies AngoiPro 100.00 ug 310.00€ Pub
1070-200 Caspase-6 Substrate VEID-pNA; Appearance Liquid Biovision 200 assays 311.85€
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22027 DNAI2 antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
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K129-400 Annexin V PE Cy5 Apoptosis Detection Kit Biovision 400 assays 828.50€
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K404-60 ApoBrdU Red DNA Fragmentation Kit Biovision 60 assays 533.00€
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M22392286 4 Methoxyindole CAS Number [4837 90 5] Molekula 1 G 352.00€
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  OP-0005-3  CytoX Violet Cell Proliferation Cytotoxicity Assay Kit (20x96 assays) EpigenTek 319.00€
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10051 Amplite™ Colorimetric Aldehyde Quantitation Kit AAT Bioquest 1 kit 280.00€
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36371 Screen Quest™ Colorimetric ELISA cAMP Assay Kit AAT Bioquest 10 plates 1 571.00€
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1113-1000 Caspase-10 Substrate AEVD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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K006-H1C-L Glutathione Colorimetric Detection kit (200 Low Cuvettes) Arbor Assays 200 Cuvette 394.00€ Pub
K200-100 CaspSCREEN Caspase Screening Kit Biovision 100 assays 521.00€
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K646-100 Glycogen Assay Kit 100 assays Biovision 100 assays 364.51€ Pub
BAH67-0100 Protease, DNASE free heat shock BSA powder, 100gm Equitech 100gm 403.20€
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1075-1000 Caspase 9 Substrate LEHD AFC Biovision 1000 assays 613.31€
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bs-1276R-A647 Rabbit Anti-ERdj5 DNAJC10 Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG Sepax 100ug Lyophilized 323.00€ Pub
K144-100 Cathepsin S Activity Assay Kit Biovision 100 assays 357.00€
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K4907-100 Visfatin (human) Elisa Assay Kit Biovision 100 assays 825.00€ Pub
M35520012 5 Chloro 1 methyl 4 nitroimidazole CAS Number [4897 25 0] Molekula 5 G 151.00€
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1013-1000 Annexin V Cy5 Reagent1000 assays Biovision 1000 assays 937.00€
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BT100 100 µl Barrier Tip Filtered Sterile 10 Neptune 4800 (in case - Racked - 96) 248.00€ Pub
K312-500 Bioluminescence Cytotoxicity Assay Kit Biovision 500 assays 201.00€
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K801-200 Luciferase Reporter Assay Kit Biovision 200 assays 143.00€ Pub
P-3002-1 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K4 EpigenTek 48 assays 461.00€ Pub
1014-200 Annexin V PE Reagent Biovision 200 assays 315.50€
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BT10XL 10 µl Extended Length Barrier Tip, Filtered, Sterile , Standard, Racked 96 Biotix 4800 374.85€ Pub
K122-25 Caspase 5 Fluorometric Assay Kit Biovision 25 assays 171.50€
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228-11410-2 Recombinant Mouse SCF KITLG Proteins Ray Biotech 10 205.00€
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P-3005-96 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K27 EpigenTek 96 assays 770.00€ Pub
1117-200 Caspase 12 Substrate ATAD AFC Biovision 200 assays 415.80€
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K256-100 Mitochondria Cytosol Fractionation Kit Biovision 100 assays 367.00€
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K709-100 Pyruvate Kinase Activity Assay Kit Biovision 100 assays 435.00€ Pub
DS-PB-00617 Rabbit Anti-Bovine DNAse 1 Antibodies Ray Biotech 1 ml 485.00€
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1102-200 Caspase-5 Substrate WEHD-pNA; Appearance Liquid Biovision 200 assays 311.85€
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228-10331-2 Recombinant E. coli HSP70 DnaK ATPase BD Proteins Ray Biotech 50 205.00€
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K180-25 CaspGLOW Fluorescein Active Caspase Staining Kit Biovision 25 assays 200.00€
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CQMT-01K CellQuanti MTT™ Cell Viability Assay Kits Bioassays 1000 230.45€ Pub
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