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Search results for: Stomach

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#33096169   2020/10/20 To Up

A study on the protease activity and structure of pepsin in the presence of atenolol and diltiazem.

Pepsin, as the main protease of the stomach, plays an important role in the digestion of food proteins into smaller peptides and performs about 20% of the digestive function. The role of pepsin in the development of gastrointestinal ulcers has also been studied for many years. Edible drugs that enter the body through the gastrointestinal tract will interact with this enzyme as one of the first targets. Continuous and long-term usage of some drugs will cause chronic contact of the drug with this protein, and as a result, the structure and function of pepsin may be affected. Therefore, the possible effect of atenolol and diltiazem on the structure and activity of pepsin was studied. The interaction of drugs with pepsin was evaluated using various experimental methods including UV-Visible spectroscopy, fluorescence spectroscopy, FTIR and enzymatic activity along with computational approaches. It was showed that after binding of atenolol and diltiazem to pepsin, the inherent fluorescence of the protein is quenched. Determination of the thermodynamic parameters of interactions between atenolol and diltiazem with pepsin indicates that the major forces in the formation of the protein-drug complexes are hydrophobic forces and also atenolol has a stronger protein bonding than diltiazem. Additional tests also show that the protease activity of pepsin, decreases and increases in the presence of atenolol and diltiazem, respectively. Investigation of the FTIR spectrum of the protein in the presence and absence of atenolol and diltiazem show that in the presence of atenolol the structure of protein has slightly changed. Molecular modeling studies, in agreement with the experimental results, confirm the binding of atenolol and diltiazem to the enzyme pepsin and show that the drugs are bind close to the active site of the enzyme. Finally, from experimental and computational results, it can be concluded that atenolol and diltiazem interact with the pepsin and change its structure and protease activity.
Sajad Moradi, Hadi Gholami, Changiz Karami, Negin Farhadian, Fatemeh Balaei, Mohabbat Ansari, Mohsen Shahlaei

2702 related Products with: A study on the protease activity and structure of pepsin in the presence of atenolol and diltiazem.

11 kit100μg50 ug

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#33095998   2020/10/23 To Up

The Intervention of tert-Butylhydroquinone Protects Ethanol-Induced Gastric Ulcer in Type-II Diabetic Rats: Role of Nrf2 pathway.

Ethanol consumption increases the prevalence of gastric ulcer (GU) in rats with type 2 diabetes (T2D). Induction of GU by absolute ethanol (5 mL/kg or 3.94 g/kg) in the animal model resembles human ulcer characteristics. The aim was to investigate the role of the Nrf2 pathway in the treatment of GU in diabetic condition. The rats were exposed to absolute ethanol before one hour of sacrifice and T2D was induced by combined exposure of high-fat diet and low dose streptozotocin. Pre-treatment of tBHQ (25 and 50 mg/kg), metformin (500 mg/kg) and omeprazole (20 mg/kg) were given once daily for last three consecutive weeks. In ethanol-exposed diabetic rats, pretreatment with tBHQ, omeprazole, and metformin reduced gastric mucosal lesion, ulcer index, histological alterations, MDA level and apoptosis. Further, the intervention of tBHQ, omeprazole and metformin improved the integrity of the stomach mucosa, glutathione, gastric pH, collagen and goblet cells. tBHQ treatment improved ethanol-induced alterations of Nrf2, catalase, HSP70, NF-κB and endothelin-1 expressions in diabetic rats. In diabetic conditions, the incidence of GU is increased due to elevated levels of ROS, inflammatory mediators, depleted levels of cellular antioxidants, altered gastric parameters. The tBHQ intervention could be a rational strategy to protect these changes.
Ziaur Rahman, Durgesh Kumar Dwivedi, G Jena

2565 related Products with: The Intervention of tert-Butylhydroquinone Protects Ethanol-Induced Gastric Ulcer in Type-II Diabetic Rats: Role of Nrf2 pathway.

96T2 Pieces/Box1 mg2 Pieces/Box1 mg5ug2 Pieces/Box100 ul100 μg

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#33095121   2020/10/23 To Up

Cancer prevalence by phase of care: an indicator for assessing health service needs.

Cancer prevalence (people alive on a certain date in a population who previously had a cancer diagnosis) is expected to increase in the United States and Europe due to improvements in survival and population aging. Examination of prevalence by phase of care allows us to identify subgroups of patients according to their care trajectories, thus allowing us to improve health care planning, resource allocation, and calculation of costs.
Anna Gigli, Silvia Francisci, Stefano Guzzinati, Aaron Hall, Mark Hachey, Steve Scoppa, Angela Mariotto

1968 related Products with: Cancer prevalence by phase of care: an indicator for assessing health service needs.

500 6 ml Ready-to-use 100μg 100ul

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#33094598   // To Up

Auto-immune gastritis induced by pembrolizumab, an anti-PD-1, in a melanoma patient.

We report a case of a 67-years-old woman presenting a severe acute lymphocytic gastritis induced by pembrolizumab, an immune check point inhibitor (ICI). This gastritis was her third auto-immune adverse event after 5 years of treatment with pembrolizumab, it was metabolically active at the PET Scan and confirmed by analysis of the gastric biopsies. Pembrolizumab treatment cessation and high doses of corticosteroids completely normalized the stomach clinically, endoscopically and histologically. This patient was in complete remission of her metastatic melanoma. Therefore, pembrolizumab therapy was not restarted and the patient is still in remission 6 months later. This strategy is supported by recent publications describing a relapse rate inferior to 10% in patients in complete remission after 2 years of immunotherapy. Particularities of this case are: rareness of this adverse event, late onset after introduction of pembrolizumab, evocative PET scan image, specific endoscopic aspect and histology. In addition, the favorable oncologic evolution of the patient after treatment cessation confirms the prolonged remission after immunotherapy.
J Vandepapelière, J Siplet, L Libbrecht, H Dano, J F Baurain, T Moreels

2163 related Products with: Auto-immune gastritis induced by pembrolizumab, an anti-PD-1, in a melanoma patient.

100 ul100 ul50 ul100ug100ug50 ul

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#33093904   2020/10/09 To Up

Bronchogenic cyst of the stomach: A case report and literature review.

Bronchogenic cyst (BC) is a rare congenital disease with pre-embryonic intestinal malformation. BC of the stomach is rare. The present study reported on the case of a 68-year-old male who presented with a spleen and stomach space mass detected incidentally upon a routine health examination. The patient underwent laparotomy. Postoperative histopathological diagnosis confirmed BC of the stomach. Postoperative recovery was smooth and the patient is currently under follow-up. A literature review suggested that BC is a rare disease and the location of the stomach is very rare. Indications of surgical intervention remain controversial for asymptomatic cases. Owing to no specific clinical or radiologic features to define the disease profile for diagnosis, surgery may be a good choice for both diagnosis and therapy if the patient's condition permits.
Bo Sun, An-Kang Wang, Hao Chen, Bao-Lin Qian, Xiao-Kang Yi, Yu Jiang, Qiu Li, Wen-Guang Fu, Jing Li

1243 related Products with: Bronchogenic cyst of the stomach: A case report and literature review.



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#33093806   // To Up

The Association of Sleep Disorders, Obesity and Sleep-Related Hypoxia with Cancer.

Sleep disorders have emerged as potential cancer risk factors.
Anna Brzecka, Karolina Sarul, Tomasz Dyła, Marco Avila-Rodriguez, Ricardo Cabezas-Perez, Vladimir N Chubarev, Nina N Minyaeva, Sergey G Klochkov, Margarita E Neganova, Liudmila M Mikhaleva, Siva G Somasundaram, Cecil E Kirkland, Vadim V Tarasov, Gjumrakch Aliev

1742 related Products with: The Association of Sleep Disorders, Obesity and Sleep-Related Hypoxia with Cancer.

251000 TESTS/0.65ml

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#33093489   2020/10/22 To Up

New intragastric sleeve technique reduces adipose tissue in pig experimental model: tomographic study.

In order to implement a new bariatric surgery technique, we verify the efficacy of intragastric sleeve to reduce weight gain and subcutaneous adipose tissue (SAT). Animals were divided into two groups: G1 (single-port intragastric sleeve) and G2 (sham group). The stomach was surgically reduced by single-port intragastric sutures to fo a gastric sleeve. Animals were submitted to computer tomography (CT) before the surgical procedure and after 18 weeks. Images were analyzed and measurements of the thickness of SAT, depth and width of the longissimus dorsi muscle and the rib eye area were made. Body weight and CT measurements were analyzed using the GLM PROC. The correlation coefficients were calculated among weight, moments and measures. There was a significant difference in weight gain, in which G1 had an average of 42.803 ± 3.206 kg, lower than G2 (45.966 ± 4.767 kg). The mean values for SAT and muscle measurements differed significantly between groups, in which G1 achieved the lowest values. All variables had significant correlations and high magnitude. Intragastric sleeve surgery induced a significant decrease of SAT. The new intragastric sleeve technique is feasible, safe and effective, mainly in reducing fat deposition, making it an important alternative in bariatric surgical treatment.
Mariana da Silva Ribeiro, Ricardo Paiva Araújo Dos Scheiba Zorron, Saulo José Quina Silva, Silvia Marcela Ruiz Cadena, Marcelo Borges Dos Santos Junior, Fernanda Antunes, Guilherme de Souza Vieira, Celia Raquel Quirino, André Lacerda de Abreu Oliveira

2885 related Products with: New intragastric sleeve technique reduces adipose tissue in pig experimental model: tomographic study.

4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo

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#33093458   2020/10/22 To Up

ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor effect in human stomach adenocarcinoma.

Currently, only a few available targeted drugs are considered to be effective in stomach adenocarcinoma (STAD) treatment. The PARP inhibitor olaparib is a molecularly targeted drug that continues to be investigated in BRCA-mutated tumors. However, in tumors without BRCA gene mutations, particularly in STAD, the effect and molecular mechanism of olaparib are unclear, which largely restricts the use of olaparib in STAD treatment. In this study, the in vitro results showed that olaparib specifically inhibited cell growth and migration, exerting antitumor effect in STAD cell lines. In addition, a ClC-3/SGK1 regulatory axis was identified and validated in STAD cells. We then found that the down-regulation of ClC-3/SGK1 axis attenuated olaparib-induced cell growth and migration inhibition. On the contrary, the up-regulation of ClC-3/SGK1 axis enhanced olaparib-induced cell growth and migration inhibition, and the enhancement effect could be attenuated by SGK1 knockdown. Consistently, the whole-cell recorded chloride current activated by olaparib presented the same variation trend. Next, the clinical data showed that ClC-3 and SGK1 were highly expressed in human STAD tissues and positively correlated (r = 0.276, P = 0.009). Furthermore, high protein expression of both ClC-3 (P = 0.030) and SGK1 (P = 0.006) was associated with poor survival rate in STAD patients, and positive correlations between ClC-3/SGK1 and their downstream molecules in STAD tissues were demonstrated via the GEPIA datasets. Finally, our results suggested that olaparib inhibited the PI3K/AKT pathway in STAD cells, and up-regulation of ClC-3/SGK1 axis enhanced olaparib-induced PI3K/AKT pathway inhibition. The animal experiments indicated that olaparib also exerted antitumor effect in vivo. Altogether, our findings illustrate that olaparib exerts antitumor effect in human STAD, and ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor effect. Up-regulation of the ClC-3/SGK1 axis may provide promising therapeutic potential for the clinical application of olaparib in STAD treatment.
Zhuoyu Gu, Liping Wang, Xiaohan Yao, Qian Long, Kaping Lee, Jieyao Li, Dongli Yue, Shuangning Yang, Yanfen Liu, Na Li, Yixin Li

2127 related Products with: ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor effect in human stomach adenocarcinoma.

100ul100 100 ul5ug

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#33092424   2020/10/22 To Up

Predictive value of thromboelastography for postoperative lower extremity deep venous thrombosis in gastric cancer complicated with portal hypertension patients.

To explore the predictive value of thromboelastography (TEG) for the occurrence of lower extremity deep venous thrombosis (LDVT) in gastric cancer combined with portal hypertension patients after operation.
Chunhong Gong, Kaihu Yu, Nianrong Zhang, Juan Huang

2152 related Products with: Predictive value of thromboelastography for postoperative lower extremity deep venous thrombosis in gastric cancer complicated with portal hypertension patients.



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#33092262   2020/10/20 To Up

Polyphenol Intake and Gastric Cancer Risk: Findings from the Stomach Cancer Pooling Project (StoP).

Phenolic compounds may exert a favorable effect on the risk of several cancer types, including gastric cancer (GC). However, selected polyphenol classes have not been adequately investigated in relation to GC. The aim of this study is to evaluate the association between the intake of polyphenols in relation to GC risk. We used data from the Stomach cancer Pooling (StoP) Project, including 10 studies from six countries (3471 GC cases and 8344 controls). We carried out an individual participant data pooled analysis using a two-stage approach. The summary odds ratios (ORs) of GC for each compound, and the corresponding 95% confidence intervals (95% CI), were computed by pooling study specific ORs obtained through multivariate logistic regression, using random effect models. Inverse associations with GC emerged for total polyphenols (OR = 0.67, 95% CI = 0.54-0.81, for the highest versus lowest quartile of intake), total flavonoids (OR = 0.73, 95% CI = 0.55-0.90), anthocyanidins (OR = 0.74, 95% CI = 0.56-0.92), flavanols (OR = 0.77, 95% CI = 0.66-0.88), flavanones (OR = 0.57, 95%CI = 0.44-0.69), total phenolic acids (OR = 0.75, 95%CI = 0.55-0.94), and hydroxybenzoic acids (OR = 0.73, 95%CI = 0.57-0.89). Results were consistent across strata of age, sex, social class, and smoking habit. Suggestive inverse associations were also found for flavonols (OR = 0.76, 95%CI = 0.51-1.01) and hydroxycinnamic acids (OR = 0.82, 95%CI = 0.58-1.06). Further investigations from longitudinal data are needed to confirm this association.
Facundo Vitelli-Storelli, Marta Rossi, Claudio Pelucchi, Matteo Rota, Domenico Palli, Monica Ferraroni, Nuno Lunet, Samantha Morais, Lizbeth López-Carrillo, David Georgievich Zaridze, Dmitry Maximovich, María Rubín García, Gemma Castaño-Vinyals, Nuria Aragonés, Manuela Garcia de la Hera, Raúl Ulises Hernández-Ramírez, Eva Negri, Rossella Bonzi, Mary H Ward, Areti Lagiou, Pagona Lagiou, Malaquías López-Cervantes, Paolo Boffetta, M Constanza Camargo, Maria Paula Curado, Zuo-Feng Zhang, Jesus Vioque, Carlo La Vecchia, Vicente Martín Sánchez

2027 related Products with: Polyphenol Intake and Gastric Cancer Risk: Findings from the Stomach Cancer Pooling Project (StoP).



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