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Search results for: TLR6

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#35032485   2022/01/12 To Up

Proteomic profiling reveals antitumor effects of RT2 peptide on a human colon carcinoma xenograft mouse model.

A comparative study of human colon HCT-116 xenograft in nude mice treated with and without peptide RT2 at high doses is performed along with a label-free proteomic analysis of the tissue in order to understand the potential mechanisms by which RT2 acts in vivo against colorectal tumors. RT2 displays no significant systematic toxicity, but reduces tumor growth after either intraperitoneal or intratumoral injection demonstrating it is a safe and efficacious antitumor agent in vivo. Of the 3196 proteins identified by label-free proteomics, 61 proteins appear only in response to RT2 and are involved in cellular processes largely localized in the cells and cell parts. Some of the proteins identified, including CFTR, Wnt7a, TIA1, PADI2, NRBP2, GADL1, LZIC, TLR6, and GPR37, have been reported to suppress tumor growth and are associated with cell proliferation, invasion, metastasis, angiogenesis, apoptosis, and immune evasion. Our work supports their role as tumor biomarkers and reveals RT2 has a complex mechanism of action in vivo.
Surachai Maijaroen, Sompong Klaynongsruang, Somrudee Reabroi, Arthit Chairoungdua, Sittiruk Roytrakul, Jureerut Daduang, Lapatrada Taemaitree, Nisachon Jangpromma

1760 related Products with: Proteomic profiling reveals antitumor effects of RT2 peptide on a human colon carcinoma xenograft mouse model.

1 mg2 ml0.2 mg1 mg100.00 ug100 0.25 ml100.00 ug100ug Lyophilized100ug Lyophilized20

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#34985402   2022/01/05 To Up

Candidate Role for Toll-like Receptor 3 L412F Polymorphism and Infection in Acute Exacerbation of Idiopathic Pulmonary Fibrosis.

The Toll-like receptor 3 Leu412Phe ( L412F) polymorphism attenuates cellular anti-viral responses and is associated with accelerated disease progression in IPF. The role of TLR3 L412F in bacterial infection in IPF or in acute exacerbations (AE) has not been reported. To characterize the association between L412F and AE-related death in IPF. To determine the effect of L412F on the lung microbiome and on anti-bacterial TLR-responses of primary lung fibroblasts from IPF patients. TLR-mediated anti-bacterial and anti-viral responses were quantitated in L412F-wild-type and 412F-heterozygous primary lung fibroblasts from IPF patients using ELISA, western blot analysis and qPCR. Hierarchical heatmap analysis was employed to establish bacterial and viral clustering in nasopharyngeal lavage (NPL) samples from AE-IPF patients. 16S rRNA qPCR and pyrosequencing were used to determine the effect of L412F on the IPF lung microbiome. A significant increase in AE-related death in 412F-variant IPF patients was reported. We established that 412F-heterozygous IPF lung fibroblasts have reduced anti-bacterial TLR responses to LPS (TLR4), Pam3CYSK4 (TLR1/2), flagellin (TLR5) and FSL-1 (TLR6/1) and have reduced responses to live Pseudomonas aeruginosa infection. Using 16S rRNA sequencing, we demonstrated that 412F-heterozygous IPF patients have a dysregulated lung microbiome with increased frequencies of Streptococcus and Staphylococcus spp. This study reveals that L412F dysregulates the IPF lung microbiome and reduces the responses of IPF lung fibroblasts to bacterial TLR-agonists and live bacterial infection. These findings identify a candidate role for L412F in viral- and bacterial-mediated AE-death.
Aoife N McElroy, Rachele Invernizzi, Joanna W Laskowska, Andrew O'Neill, Mohammad Doroudian, Mohsen Moghoofei, Shayan Mostafaei, Feng Li, Alexander A Przybylski, David N O'Dwyer, Andrew G Bowie, Padraic G Fallon, Toby M Maher, Cory M Hogaboam, Philip L Molyneaux, Nik Hirani, Michelle E Armstrong, Seamas C Donnelly

2477 related Products with: Candidate Role for Toll-like Receptor 3 L412F Polymorphism and Infection in Acute Exacerbation of Idiopathic Pulmonary Fibrosis.

5mg100ug Lyophilized1 g96T250 mg96 wells (1 kit)100ug100ug100ug Lyophilized100ug100ug

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#34930972   2021/12/20 To Up

The effect of toll-like receptor ligands on energy metabolism and myokine expression and secretion in cultured human skeletal muscle cells.

Skeletal muscle plays an important role in glycaemic control and metabolic homeostasis, making it a tissue of interest with respect to type 2 diabetes mellitus. The aim of the present study was to determine if ligands of Toll-like receptors (TLRs) could have an impact on energy metabolism and myokine expression and secretion in cultured human skeletal muscle cells. The myotubes expressed mRNA for TLRs 1-6. TLR3, TLR4, TLR5 and TLR6 ligands (TLRLs) increased glucose metabolism. Furthermore, TLR4L and TLR5L increased oleic acid metabolism. The metabolic effects of TLRLs were not evident until after at least 24 h pre-incubation of the cells and here the metabolic effects were more evident for the metabolism of glucose than oleic acid, with a shift towards effects on oleic acid metabolism after chronic exposure (168 h). However, the stimulatory effect of TLRLs on myokine expression and secretion was detected after only 6 h, where TLR3-6L stimulated secretion of interleukin-6 (IL-6). TLR5L also increased secretion of interleukin-8 (IL-8), while TLR6L also increased secretion of granulocyte-macrophage colony stimulating factor (GM-CSF). Pre-incubation of the myotubes with IL-6 for 24 h increased oleic acid oxidation but had no effect on glucose metabolism. Thus IL-6 did not mimic all the metabolic effects of the TLRLs, implying metabolic effects beyond the actions of this myokine.
Ragna H Tingstad, Frode Norheim, Fred Haugen, Yuan Z Feng, Hege S Tunsjø, G Hege Thoresen, Arild C Rustan, Colin Charnock, Vigdis Aas

2719 related Products with: The effect of toll-like receptor ligands on energy metabolism and myokine expression and secretion in cultured human skeletal muscle cells.

96 tests100ul1 ml200 1000 0.1 mg100.00 ul1.00 flask100 extractions5mg100

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#34922572   2021/12/18 To Up

Alleviating the effect of quinoa and the underlying mechanism on hepatic steatosis in high-fat diet-fed rats.

Nonalcoholic fatty liver disease (NAFLD) is considered the hepatic component of metabolic syndrome and has attracted widespread attention due to its increased prevalence. Daily dietary management is an effective strategy for the prevention of NAFLD. Quinoa, a nutritious pseudocereal, is abundant in antioxidative bioactive phytochemicals. In the present study, the effects of different amounts of quinoa on the progression of NAFLD and the related molecular mechanism were investigated.
Chenwei Song, Wei Lv, Yahui Li, Pan Nie, Jun Lu, Yanlou Geng, Zhang Heng, Lihua Song

2194 related Products with: Alleviating the effect of quinoa and the underlying mechanism on hepatic steatosis in high-fat diet-fed rats.

15mg1 ml

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#34878713   2021/12/08 To Up

Lactobacillus acidophilus PIN7 paraprobiotic supplementation ameliorates DSS-induced colitis through anti-inflammatory and immune regulatory effects.

This study aimed to evaluate the efficacy of paraprobiotics Lactobacillus acidophilus PIN7 supplementation against dextran sodium sulphate (DSS)-induced colitis in mice and to determine their mechanisms of the action.
Yeon-Jin Kye, So-Young Lee, Ha-Ra Kim, Byung-Hoo Lee, Jong-Hyun Park, Myeong-Soo Park, Geun-Eog Ji, Mi-Kyung Sung

2554 related Products with: Lactobacillus acidophilus PIN7 paraprobiotic supplementation ameliorates DSS-induced colitis through anti-inflammatory and immune regulatory effects.

200 0.1 mg1000 100ul1 ml50 100ug100ul100 50 ul100ug100ug

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#34866574   2021/12/06 To Up

SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway.

The pathogenesis of COVID-19 is associated with a hyperinflammatory response; however, the precise mechanism of SARS-CoV-2-induced inflammation is poorly understood. Here, we investigated direct inflammatory functions of major structural proteins of SARS-CoV-2. We observed that spike (S) protein potently induced inflammatory cytokines and chemokines, including IL-6, IL-1β, TNFα, CXCL1, CXCL2, and CCL2, but not IFNs in human and mouse macrophages. No such inflammatory response was observed in response to membrane (M), envelope (E), and nucleocapsid (N) proteins. When stimulated with extracellular S protein, human and mouse lung epithelial cells also produced inflammatory cytokines and chemokines. Interestingly, epithelial cells expressing S protein intracellularly were non-inflammatory, but elicited an inflammatory response in macrophages when co-cultured. Biochemical studies revealed that S protein triggers inflammation via activation of the NF-κB pathway in a MyD88-dependent manner. Further, such an activation of the NF-κB pathway was abrogated in Tlr2-deficient macrophages. Consistently, administration of S protein-induced IL-6, TNF-α, and IL-1β in wild-type, but not Tlr2-deficient mice. Notably, upon recognition of S protein, TLR2 dimerizes with TLR1 or TLR6 to activate the NF-κB pathway. Taken together, these data reveal a mechanism for the cytokine storm during SARS-CoV-2 infection and suggest that TLR2 could be a potential therapeutic target for COVID-19.
Shahanshah Khan, Mahnoush S Shafiei, Christopher Longoria, John W Schoggins, Rashmin C Savani, Hasan Zaki

1095 related Products with: SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway.

100 0.1 mg100 20 ug Product tipe: Antib100 300 100 200 100 10025μg/vial250ul

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#34754960   2021/10/02 To Up

Rumen-protected glucose supplementation in transition dairy cows shifts fermentation patterns and enhances mucosal immunity.

Manipulation of perinatal diets, such as supplementing feed with rumen-protected glucose (RPG), has been positively regarded as a strategy to improve milking performance. This study was conducted to assess the effects of RPG on the fermentation profiles, resident microbiota and mucosal immunity in the cecum. Ten Holstein dairy cows were randomly assigned to either a 25 g/kg RPG diet (DM basis) or a 11 g/kg coating fat diet (control, CON). Compared with the CON group, the acetate-to-propionate ratio was lower in the RPG group. Gene expression analysis indicated that RPG supplementation tended to upregulate the expression of Na/H hydrogen exchanger 3 () ( = 0.076). RPG supplementation downregulated the expression of genes involved in self-rehabilitation such as matrix metalloproteinase 1 (), , and . Additionally, the mRNA expression of genes involved in immunity including Toll-like receptors (, and ) and proinflammatory cytokines (immune interferon gamma [] and interleukins interleukin 17A [], , ), was downregulated by RPG supplementation. Nonetheless, no differences existed in the bacterial copy number and beta diversity between the 2 groups. Overall, supplementation with RPG would probably cause a shift towards propionate production in the cecal digesta, and promote the immune homeostasis of the cecal mucosa in transition dairy cows. Our results extended the basic understanding of RPG supplementation and utilization in transition dairy cows in terms of host microbe interplay in the cecum.
Xiaoli Zhang, Xiaopeng Li, Jian Wu, Jinzhen Jiao, Zhixiong He, Zhiliang Tan, Xuefeng Han

2431 related Products with: Rumen-protected glucose supplementation in transition dairy cows shifts fermentation patterns and enhances mucosal immunity.

2 Pieces/Box96 assays100 assays100 assays1 Product tipe: Instrumen100000 Units96 wells (1 kit)100ug Lyophilized1 Set

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#34675627   2021/10/12 To Up

Construction and Investigation of Competing Endogenous RNA Networks and Candidate Genes Involved in SARS-CoV-2 Infection.

The current COVID-19 pandemic caused by a novel coronavirus SARS-CoV-2 is a quickly developing global health crisis, yet the mechanisms of pathogenesis in COVID-19 are not fully understood.
Mingran Qi, Bin Liu, Shuai Li, Zhaohui Ni, Fan Li

1876 related Products with: Construction and Investigation of Competing Endogenous RNA Networks and Candidate Genes Involved in SARS-CoV-2 Infection.

200ul200ul200ul10 mg200ul200ug200ug200ul200ul25 mg100ul250ul

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#34636427   2021/10/12 To Up

Cryopreservation alters the immune characteristics of platelets.

Cryopreserved platelets are under clinical evaluation as they offer improvements in shelf-life and potentially hemostatic effectiveness. However, the effect of cryopreservation on characteristics related to the immune function of platelets has not been examined.
Ben Wood, Matthew P Padula, Denese C Marks, Lacey Johnson

2599 related Products with: Cryopreservation alters the immune characteristics of platelets.

12100 ug50 IU100.00 ul 5 G500 Units100ug1 ml1

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#34626426   2021/10/20 To Up

The soluble cytoplasmic tail of CD45 regulates T-cell activation via TLR4 signaling.

The soluble cytoplasmic tail of CD45 (ct-CD45) is a cleavage fragment of CD45, that is generated during the activation of human phagocytes. Upon release to the extracellular space, ct-CD45 binds to human T cells and inhibits their activation in vitro. Here, we studied the potential role of TLR4 as a receptor for ct-CD45. Treatment of Jurkat TLR4/CD14 reporter cells with ct-CD45 induced the upregulation of the reporter gene NFκB-eGFP and could be blocked by inhibitors of TLR4 signaling. Conversely, ct-CD45 did not promote the NFκB-controlled eGFP induction in reporter cells expressing TLR1, TLR2, and TLR6 transgenes and did not lead to the activation of the transcription factors NFκB, AP-1, and NFAT in a Jurkat reporter cell line expressing endogenous TLR5. Moreover, ct-CD45 binds to recombinant TLR4 in an in vitro assay and this association was reduced in the presence of oxidized 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine. Blockade of TLR4 with mAb HTA125 partially reversed the ct-CD45-mediated inhibition of T-cell proliferation. Interestingly, targeting of TLR4 with mAb W7C11 also suppressed T-cell proliferation. In summary, the results of this study demonstrate that ct-CD45 acts via a noncanonical TLR4 activation pathway on T cells, which modulates TCR signaling.
Alexander Puck, Sarojinidevi Künig, Madhura Modak, Lara May, Pia Fritz, Claire Battin, Katharina Radakovics, Peter Steinberger, Birgit M Reipert, Brian A Crowe, Johannes Stöckl

1767 related Products with: The soluble cytoplasmic tail of CD45 regulates T-cell activation via TLR4 signaling.

1 vial61000 tests100ìl x 10 vials5 x 2 mlOne Vial: 5 X 10^6 Cells2500 assays1 kit1000 assays25 x 2 ml

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