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Search results for: Theophylline

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#34126330   2021/06/07 To Up

Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFeO/CoFeO/NCDs nanospheres: Fabrication, application and DFT theoretical study.

Hollow peanut-shaped NiFeO/CoFeO twinned nano-spherical shell composite materials have interconnected electron channels and excellent electrochemical performance, which prompted the use of this unique spatial structure to fabricate efficient electrochemical sensors. In this work, N-doped carbon dots (NCDs) incorporated into magnetic NiFeO/CoFeO nanoparticle shell (NiFeO/CoFeO/NCDs) modified glassy carbon electrode (GCE) was applied to construct a dual-template molecularly imprinted polymer (MIP) based electrochemistry sensor (NiFeO/CoFeO/NCDs/MIP/GCE) for the simultaneous detection of catechin (CA) and theophylline (TPH). MIP was fabricated by an in-situ electrochemical polymerization strategy based on the theoretical exploration and density functional theory (DFT) computer directional simulation to screen out the optimal functional monomer (L-arginine) and the optimal ratio between the dual template molecules (CA and TPH) and functional monomer. The materials were characterized by SEM, TEM, XRD, XPS, and TGA. Besides, electron binding energy, binding constant, and imprinting factor were investigated. With the optimal conditions, the proposed electrochemical dual detection system showed outstanding analytical performance for the simultaneous sensing of CA and TPH, with an ultralow detection limit (LOD, S/N = 3) of 1.3 nM for CA in 0.01-1 μM (R = 0.9956) and 1-50 μM (R = 0.9928), as well as a LOD of 20.0 nM for TPH in the linear range of 0.1-100 μM (R = 0.9939), respectively. Also, the selectivity and anti-interference performances of the fabricated sensor were performed by differential pulse voltammetry and chronoamperometry, and successfully detected the analyte from tea drinks and human urine samples with the recovery rates ranging from 98.22% to 104.76% and relative standard deviations (RSD) were 1.19%-3.81%, demonstrated the sensor has excellent stability, repeatability, and reproducibility, which paves the way for other platforms to use this nanomaterial for the detection of antioxidant in the filed food safety.
Zhiwei Lu, Xin Du, Mengmeng Sun, Yan Zhang, Yifan Li, Xianxiang Wang, Yanying Wang, Haijun Du, Huadong Yin, Hanbing Rao

2315 related Products with: Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFeO/CoFeO/NCDs nanospheres: Fabrication, application and DFT theoretical study.

0.25 mg0.1ml (1mg/ml) 1 G 100ul0.1 ml0.1ml (1mg/ml)0.1 mg 1L 5 G 100ul

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#34112714   2021/06/10 To Up

Methylxanthines in COPD: yes to caffeine, no to theophylline.


Ken M Kunisaki, Don D Sin

2950 related Products with: Methylxanthines in COPD: yes to caffeine, no to theophylline.

100 mg96 wells501 kit1050 µg

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#34111540   2021/06/07 To Up

Antinociceptive Effect of The Hydroethanolic Leaf Extract of Calotropis procera (Ait) R. Br. (Apocynaceae): possible involvement of glutamatergic, cytokines, opioidergic and adenosinergic pathways.

Pain remains real and still a major problem in clinical medicine which requires new agents with improved efficacy for more therapeutic benefits. Plant sources can serve as a basis for the search for some novel drugs hence the analgesic effects of the hydroethanolic extract of Calotropis procera (CPE) which is widespread in Ghana and other tropical areas and used in folkloric medicine for painful and inflammatory conditions was evaluated.
Ernest Obese, Robert P Biney, Isaac T Henneh, Daniel Anokwah, Emmanuel A Adakudugu, Eric Woode, Elvis O Ameyaw

2346 related Products with: Antinociceptive Effect of The Hydroethanolic Leaf Extract of Calotropis procera (Ait) R. Br. (Apocynaceae): possible involvement of glutamatergic, cytokines, opioidergic and adenosinergic pathways.

5 G100ul100 extractions16 Arrays/Slide1 Kit2 Membrane supply1 mgmin 2 cartons16 Arrays/Slide200 extractions2 Membrane supply

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#34106311   2021/06/09 To Up

Synthetic counter-selection markers and their application in genetic modification of Synechococcus elongatus UTEX2973.

Due to its robustness to environmental stresses and fast growth, Synechococcus elongatus UTEX2973 is developed as a new model for researches on cyanobacterial molecular biology and biotechnology. However, systematic genetic modifications of S. elongatus UTEX2973 were hindered by the lack of effective genetic manipulation tools, especially available counter-selection markers. Here, six synthetic counter-selection markers (SCOMs) were assembled by fusing six toxin genes from either Escherichia coli or cyanobacteria with a theophylline-inducible promoter. The SCOMs containing SYNPCC7002_G0085 from Synechococcus sp. PCC7002 or mazF from E. coli were proved to be inducible by theophylline in S. elongatus UTEX2973. By using the mazF-based SCOM, the neutral locus 1 and 23 small regulatory RNAs were completely deleted from the genome of S. elongatus UTEX2973 after one round of selection with both kanamycin and theophylline. The genetic tools developed in this work will facilitate future researches on molecular genetics and synthetic biology in S. elongatus UTEX2973. KEY POINTS: • Two inducible counter-selection markers are lethal to S. elongatus UTEX2973. • The counter-selection marker benefits the gene targeting in S. elongatus UTEX2973. • Twentry-three small regulatory RNAs were fully deleted via the novel gene targeting method.
Liyuan Chen, Hai Liu, Li Wang, Xiaoming Tan, Shihui Yang

1068 related Products with: Synthetic counter-selection markers and their application in genetic modification of Synechococcus elongatus UTEX2973.

100ul100ug Lyophilized0.1ml 100ul0.1ml0.1ml (1mg/ml) 100ul 100ul100ug Lyophilized 100ul 100ul

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#34093387   2021/05/21 To Up

Machine Learning-Based Prediction of Brain Tissue Infarction in Patients With Acute Ischemic Stroke Treated With Theophylline as an Add-On to Thrombolytic Therapy: A Randomized Clinical Trial Subgroup Analysis.

The theophylline in acute ischemic stroke trial investigated the neuroprotective effect of theophylline as an add-on to thrombolytic therapy in patients with acute ischemic stroke. The aim of this pre-planned subgroup analysis was to use predictive modeling to virtually test for differences in the follow-up lesion volumes. A subgroup of 52 patients from the theophylline in acute ischemic stroke trial with multi-parametric MRI data acquired at baseline and at 24-h follow-up were analyzed. A machine learning model using voxel-by-voxel information from diffusion- and perfusion-weighted MRI and clinical parameters was used to predict the infarct volume for each individual patient and both treatment arms. After training of the two predictive models, two virtual lesion outcomes were available for each patient, one lesion predicted for theophylline treatment and one lesion predicted for placebo treatment. The mean predicted volume of follow-up lesions was 11.4 ml (standard deviation 18.7) for patients virtually treated with theophylline and 11.2 ml (standard deviation 17.3) for patients virtually treated with placebo ( = 0.86). The predicted follow-up brain lesions for each patient were not significantly different for patients virtually treated with theophylline or placebo, as an add-on to thrombolytic therapy. Thus, this study confirmed the lack of neuroprotective effect of theophylline shown in the main clinical trial and is contrary to the results from preclinical stroke models.
Boris Modrau, Anthony Winder, Niels Hjort, Martin Nygård Johansen, Grethe Andersen, Jens Fiehler, Henrik Vorum, Nils D Forkert

1032 related Products with: Machine Learning-Based Prediction of Brain Tissue Infarction in Patients With Acute Ischemic Stroke Treated With Theophylline as an Add-On to Thrombolytic Therapy: A Randomized Clinical Trial Subgroup Analysis.



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#34082959   2021/04/30 To Up

Tuning bio-aerogel properties for controlling theophylline delivery. Part 1: Pectin aerogels.

A comprehensive study of release kinetics of a hydrophilic drug from bio-aerogels based on pectin was performed. Pectin aerogels were made by polymer dissolution, gelation (in some cases this step was omitted), solvent exchange and drying with supercritical CO. Theophylline was loaded and its release was studied in the simulated gastric fluid during 1 h followed by the release in the simulated intestinal fluid. Pectin concentration, initial solution pH and concentration of calcium were varied to tune the properties of aerogel. The kinetics of theophylline release was monitored and correlated with aerogel density, specific surface area, and aerogel swelling and erosion. Various kinetic models were tested to identify the main physical mechanisms governing the release.
Sophie Groult, Sytze Buwalda, Tatiana Budtova

1622 related Products with: Tuning bio-aerogel properties for controlling theophylline delivery. Part 1: Pectin aerogels.

1 G 2x5L96 tests 100 G0.25 mg1.00 ml1 g50 ml50 mL

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#34078115   2021/06/02 To Up

Impact of Interferons and Biological Drug Inhibitors of IL-2 and IL-6 on Small-Molecule Drug Metabolism Through the Cytochrome P450 System.

Assess the impact of interferons and interleukin (IL)-2 and IL-6 inhibitors on cytochrome P450 (CYP) drug metabolism in human subjects.
C Michael White, Dakota J Sicignano, Kimberly Smith

1440 related Products with: Impact of Interferons and Biological Drug Inhibitors of IL-2 and IL-6 on Small-Molecule Drug Metabolism Through the Cytochrome P450 System.

200ul1000 TESTS/0.65ml100 mg10 mg250ul200ul25 mg200ug250ul25 mg200ug

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#34074405   2021/04/20 To Up

A malachite green light-up aptasensor for the detection of theophylline.

Biosensors are of interest for the quantitative detection of small molecules (metabolites, drugs and contaminants for instance). To this end, fluorescence is a widely used technique that is easily associated to aptamers. Light-up aptamers constitute a particular class of oligonucleotides that, specifically induce fluorescence emission when binding to cognate fluorogenic ligands such as malachite green (MG). We engineered a dual aptasensor for theophylline (Th) based on the combination of switching hairpin aptamers specific for MG on the one hand and for Th on the other hand, hence their names: malaswitch (Msw) and theoswitch (Thsw). The two aptaswitches form a loop-loop or kissing Msw-Thsw complex only in the presence of theophylline, allowing binding of MG, subsequently generating a fluorescent signal. The combination of the best Msw and Thsw variants, MswG12 and Thsw19.1, results in a 20-fold fluorescence enhancement of MG at saturating theophylline concentration. This aptasensor discriminates between theophylline and its analogues caffeine and theobromine. Kissing aptaswitches derived from light-up aptamers constitute a novel sensing device.
Arghya Sett, Lorena Zara, Eric Dausse, Jean-Jacques Toulmé

1585 related Products with: A malachite green light-up aptasensor for the detection of theophylline.

1 kit25001 ml1 ml 100 G0.1 ml50 100tests100 ml

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#34056349   2021/04/30 To Up

Synthesis, Hemolytic Studies, and Modeling of Novel Acefylline-1,2,4-Triazole Hybrids as Potential Anti-cancer Agents against MCF-7 and A549.

A series of novel theophylline-7-acetic acid (acefylline)-derived 1,2,4-triazole hybrids with -phenyl acetamide moieties (-) have been synthesized and tested for their inhibitory () potential against two cancer cell lines, A549 (lung) and MCF-7 (breast), using MTT assay. Among these derivatives, , , , , and displayed remarkable activity against both cancer cell lines having cell viability values in the 21.74 ± 1.60-55.37 ± 4.60% range compared to acefylline (86.32 ± 1.75%) using 100 μg/μL concentration of compounds. These compounds were further screened against the A549 cancer cell line (lung) to find their half-maximal inhibitory concentration (IC) by applying various concentrations of these compounds. Compound (2-(5-((1,3-dimethyl-2,6-dioxo-2,3-dihydro-1-purin-7(6)-yl)methyl)-4-phenyl-4-1,2,4-triazol-3-ylthio)---tolylacetamide) with the least IC value (1.25 ± 1.36 μM) was discerned as a strong inhibitor of cancer cell multiplication in both cell lines (A549 and MCF-7). Their hemolytic studies revealed that all of them had very low cytotoxicity. Finally, modeling was carried out to find the mode of binding of the highly active compound (), which was according to the results of anti-cancer activity.
Irum Shahzadi, Ameer Fawad Zahoor, Azhar Rasul, Asim Mansha, Sajjad Ahmad, Zohaib Raza

2831 related Products with: Synthesis, Hemolytic Studies, and Modeling of Novel Acefylline-1,2,4-Triazole Hybrids as Potential Anti-cancer Agents against MCF-7 and A549.

0.1ml (1mg/ml)1 ml100.00 ul1000 TESTS/0.65ml100ul200 1 mg100ul1000 0.1 mg

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#34046723   // To Up

[Post-dural puncture headache].

Post-dural puncture headache belongs to the group of secondary headache disorders and is a typical complication of intended or unintended dural puncture. The main symptom is orthostatic headache, which can be accompanied by neurological symptoms such as diplopia depending on the extent of the cerebrospinal fluid leak. The course of this headache is predominantly benign, showing spontaneous improvement over a couple of days, although severe cases are described in the literature. The following article provides an overview of the current knowledge about the headache's pathophysiology, diagnostic work-up and therapy.
Katharina Kamm, Stefanie Förderreuther

2902 related Products with: [Post-dural puncture headache].

500 ml 1000 ml

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