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#33073913 2020/10/19 To Up
Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana.Systemic acquired resistance (SAR) is a broad-spectrum disease resistance response that can be induced upon infection from pathogens or by chemical treatment, such as with benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH). SAR involves priming for more robust activation of defence genes upon pathogen attack. Whether priming for SAR would involve components of RNA silencing remained unknown. Here, we show that upon leaf infiltration of water, BTH-primed Arabidopsis thaliana plants accumulate higher amounts of mRNA of ARGONAUTE (AGO)2 and AGO3, key components of RNA silencing. The enhanced AGO2 expression is associated with prior-to-activation trimethylation of lysine 4 in histone H3 and acetylation of histone H3 in the AGO2 promoter and with induced resistance to the yellow strain of cucumber mosaic virus (CMV[Y]). The results suggest that priming A. thaliana for enhanced defence involves modification of histones in the AGO2 promoter that condition AGO2 for enhanced activation, associated with resistance to CMV(Y). Consistently, the fold-reduction in CMV(Y) coat protein accumulation by BTH pretreatment was lower in ago2 than in wild type, pointing to reduced capacity of ago2 to activate BTH-induced CMV(Y) resistance. A role of AGO2 in pathogen-induced SAR is suggested by the enhanced activation of AGO2 after infiltrating systemic leaves of plants expressing a localized hypersensitive response upon CMV(Y) infection. In addition, local inoculation of SAR-inducing Pseudomonas syringae pv. maculicola causes systemic priming for enhanced AGO2 expression. Together our results indicate that defence priming targets the AGO2 component of RNA silencing whose enhanced expression is likely to contribute to SAR.
Sugihiro Ando, Michal Jaskiewicz, Sei Mochizuki, Saeko Koseki, Shuhei Miyashita, Hideki Takahashi, Uwe Conrath
2109 related Products with: Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana.2ug5ug100 100ug100ug100 µg25100 200 25 1 mL50 ul
#33073894 2020/10/19 To Up
Dynamic Helicates Self-Assembly from Homo- and Heterotopic Dynamic Covalent Ligand Strands.The understanding and the application of reversible covalent reactions and coordination chemistry together with the proper design of the molecular frameworks, allow to achieve not only well-defined output architectures but also different grades of complex behavior. In this work, the dynamic nature of the helical systems offers an additional level of complexity by combining self-sorting on two levels: 1) the build-up of the ligand strand constituents from their components through dynamic covalent chemistry; 2) the assembly of the helicates from the ligands and the metal cations through dynamic metallo-supramolecular chemistry. The information encoded in the ligands constituent molecule was read differently (and accurately at the same time) by metal cations that varied in the coordination algorithms. It enabled the selective formation of a specific type of helicates from a wide library of helicates formed by the possible combination of subcomponents. Ligands containing dynamic tridentate and/or bidentate binding motifs in the same strand were studied to explore the helicates self-assembly with appropriate metal cations.
Antonio Santoro, Jan Holub, Marta A Fik-Jaskółka, Ghislaine Vantomme, Jean-Marie Lehn
2189 related Products with: Dynamic Helicates Self-Assembly from Homo- and Heterotopic Dynamic Covalent Ligand Strands.1 mg 5 G100 10 mg100ul250 2ug10 mg96T
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#33073883 2020/10/19 To Up
Outcomes of monochorionic twin pregnancies complicated by Type-III selective fetal growth restriction.Type III selective intrauterine growth restriction (sIUGR) is associated with a high and unpredictable risk of fetal death and fetal brain injury. Our objective was to describe the prospective risk of fetal death and the risk of adverse neonatal outcomes in a contemporary cohort.
S Shinar, W Xing, V Pruthi, C Jianping, F Slaghekke, S Groene, E Lopriore, L Lewi, I Couck, Y Yinon, L Batsry, L Raio, S Amylidi-Mohr, D Baud, F Kneuss, P DeKoninck, J Moscou, J Barrett, N Melamed, G Ryan, L Sun, T Van Mieghem
2716 related Products with: Outcomes of monochorionic twin pregnancies complicated by Type-III selective fetal growth restriction.100.00 mg100.00 ug100ul2ug50 ml96T50
#33073872 2020/10/19 To Up
Functional analyses of STIM1 mutations reveal a common pathomechanism for tubular aggregate myopathy and Stormorken syndrome.Tubular aggregate myopathy (TAM) is a progressive disorder characterized by muscle weakness, cramps, and myalgia. TAM clinically overlaps with Stormorken syndrome (STRMK), combining TAM with miosis, thrombocytopenia, hyposplenism, ichthyosis, short stature, and dyslexia. TAM and STRMK arise from gain-of-function mutations in STIM1 (stromal interaction molecule 1) or ORAI1, both encoding key regulators of Ca homeostasis, and mutations in either gene result in excessive extracellular Ca entry. The pathomechanistic similarities and differences between TAM and STRMK are only partially understood. Here we provide functional in vitro experiments demonstrating that STIM1 harboring the TAM D84G or the STRMK R304W mutation similarly cluster and exert a dominant effect on the wild-type protein. Both mutants recruit ORAI1 to the clusters, increase cytosolic Ca levels, promote major nuclear import of the Ca -dependent transcription factor NFAT (nuclear factor of activated T cells), and trigger the formation of circular membrane stacks. In conclusion, the analyzed TAM and STRMK mutations have a comparable impact on STIM1 protein function and downstream effects of excessive Ca entry, highlighting that TAM and STRMK involve a common pathomechanism.
Georges Arielle Peche, Coralie Spiegelhalter, Roberto Silva-Rojas, Jocelyn Laporte, Johann Böhm
1463 related Products with: Functional analyses of STIM1 mutations reveal a common pathomechanism for tubular aggregate myopathy and Stormorken syndrome.1 mg100ug1 kit(96 Wells)25 g25 mg 1 G250 ml0.1 ml 5 G 6 ml Ready-to-use 100ug
#33073862 2020/10/19 To Up
The multiple meanings of omnivory influence empirical, modular theory, and whole food web stability relationships.The persistence of whole communities hinges on the presence of select interactions which act to stabilize communities making the identification of these keystone interactions critical. One potential candidate is omnivory, yet theoretical research on omnivory thus far has been dominated by a modular theory approach whereby an omnivore and consumer compete for a shared resource. Empirical research, however, has highlighted the presence of a broader suite of omnivory modules. Here, we integrate empirical data analysis and mathematical models to explore the influence of both omnivory module (including classic, multi-resource, higher level, mutual predation, and cannibalism) and omnivore-resource interaction type on food web stability. We use six classic empirical food webs to examine the prevalence of the different types of omnivory, a multi-species consumer-resource model to determine the stability of these different kinds of omnivory within a module context, and finally extend these models to a 50 species, whole food web model to examine the influence of omnivory on whole food web persistence. Our results challenge the concept that omnivory is broadly stabilizing. In particular, we demonstrate that the impact of omnivory depends on the type of omnivory being examined with multi-resource omnivory having the largest correlation with whole food web persistence. Moreover, our results highlight that we need to exercise caution when scaling modular theory to whole food web theory. Cannibalism, for example, was the most persistent and stable omnivory module in the modular theory analysis, but only demonstrated a weak correlation with whole food web persistence. Lastly, our results demonstrate that the frequency of omnivory modules are more important for whole food web persistence than the frequency of omnivore-resource interactions. Together, these results demonstrate that the role of omnivory often depends both on the type of omnivory being examined and the food web within which it is nested. In whole food web models omnivory acts less as a keystone interaction, rather, specific types of omnivory, particularly multi-resource omnivory, act as keystone modules. Future work integrating module and whole food web theory is critical for resolving the role of key interactions in food webs.
Anne M McLeod, Shawn J Leroux
1623 related Products with: The multiple meanings of omnivory influence empirical, modular theory, and whole food web stability relationships.100.00 ul
#33073853 2020/10/19 To Up
Commentary on: Outcomes of amputation due to long-standing therapy-resistant complex regional pain syndrome type I.
Marc Russo, Peter Georgius, Willem Volschenk, Danielle Santarelli
1900 related Products with: Commentary on: Outcomes of amputation due to long-standing therapy-resistant complex regional pain syndrome type I.500 reactions1 mg1 mL100 reactions1 mg200 200 1 ml96T1 mg96T100ug
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#33073852 2020/10/19 To Up
Dust exposure and the impact on hospital readmission of farming and wood industry workers for asthma and chronic obstructive pulmonary disease (COPD).Objectives It is still not well established how occupational air pollutants affect the prognosis of asthma or chronic obstructive pulmonary disease (COPD). This study uses nationwide Danish registers and quantitative dust industry exposure matrices (IEM) for the farming and wood industries to estimate whether previous year dust exposure level impacts hospital readmissions for workers diagnosed with asthma or COPD. Methods We identified all individuals with a first diagnosis of either asthma (769 individuals) or COPD (342 individuals) between 1997 and 2007 and followed them until the next hospital admission for asthma or COPD, emigration, death or 31 December 2007. We included only individuals who worked in either the wood or farming industries at least one year during follow-up. We used logistic regression analysis to investigate associations between dust exposure level in the previous year and hospital readmission, adjusting for sex, age, time since first diagnosis, socioeconomic status, and labor force participation. Results Asthma readmissions for individuals with low and high dust exposure were increased [adjusted rate ratio (RR ) 2.52, 95% confidence interval (CI) 1.45-4.40] and RR 2.64 (95% CI 1.52-4.60), respectively. For COPD readmission, the risk estimates were RR 1.36 (95% CI 0.57-3.23) for low and RR 1.20 (95% CI 0.49-2.95) for high exposure level in the previous year. For asthma readmission, stratified analyses by type of dust exposure during follow-up showed increased risks for both wood dust [RR 2.67 (95% CI 1.35-5.26) high exposure level] and farming dust [RR 3.59 (95% CI 1.11-11.59) high exposure level]. No clear associations were seen for COPD readmissions. Conclusions This study indicates that exposure to wood or farm dust in the previous year increases the risk of hospital readmission for individuals with asthma but not for those with COPD.
Anne Vested, Henrik A Kolstad, Ioannis Basinas, Alex Burdorf, Grethe Elholm, Dick Heederik, Gitte H Jacobsen, Hans Kromhout, Øyvind Omland, Inger Schaumburg, Torben Sigsgaard, Jesper M Vestergaard, Inge M Wouters, Vivi Schlünssen
1167 related Products with: Dust exposure and the impact on hospital readmission of farming and wood industry workers for asthma and chronic obstructive pulmonary disease (COPD).25 mg10 mg500 MG25 mg96T100ul1000 TESTS/0.65ml50 mg1000 tests100ug100ug50 ug
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#33073844 2020/10/19 To Up
Transporter tandems - precise tools for normalizing active transporter in the plasma membrane.The transport efficiency (TE) describes the performance of a transport protein for a specific substrate. To compare the TE of different transporters, the number of active transporters in the plasma membrane must be monitored, as it may vary for each transporter and experiment. Available methods, like LC-MS quantification of tryptic peptides, fail to discriminate inactive intracellular transporters or, like cell-surface biotinylation followed by affinity chromatography and Western blotting, are imprecise and very laborious. We wanted to normalize active transporters by the activity of a second transporter. A transporter tandem, generated by joining 2 transporter cDNAs into a single open reading frame, should guarantee a 1:1 stoichiometry. Here we created a series of tandems with different linkers between the human ergothioneine (ET) transporter ETT (gene symbol SLC22A4) and organic cation transporter OCT2 (SLC22A2). The linker sequence strongly affected the expression strength. The stoichiometry was validated by absolute peptide quantification and untargeted peptide analysis. Compared to wild-type ETT, the normalized ET clearance of the natural variant L503F was higher (f = 1.34); G462E was completely inactive. The general usefulness of the tandem strategy was demonstrated by linking several transporters with ETT; every construct was active in both parts. Transporter tandems can be used - without membrane isolation or protein quantification - as precise tools for transporter number normalization, to identify, for example, relevant transporters for a drug. It is necessary, however, to find suitable linkers, to check the order of transporters, and to verify the absence of functional interference by saturation kinetics.
Julia Tschirka, Markus Bach, Ilmars Kisis, Julia Lemmen, Mark Jean Gnoth, Dirk Gründemann
2800 related Products with: Transporter tandems - precise tools for normalizing active transporter in the plasma membrane.4 Membranes/Box4 Membranes/Box250ul100ug Lyophilized2 Pieces/Box4 Membranes/Box96T200ul
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