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Search results for: Viability

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#32746304   2020/06/25 To Up

Vocal Imagery vs Intention: Viability of Vocal Based EEG-BCI Paradigms.

The viability of electroencephalogram (EEG) based vocal imagery (VIm) and vocal intention (VInt) Brain-Computer Interface (BCI) systems has been investigated in this study. Four different types of experimental tasks related to humming has been designed and exploited here. They are: (i) non-task specific (NTS), (ii) motor task (MT), (iii) VIm task, and (iv) VInt task. EEG signals from seventeen participants for each of these tasks were recorded from 16 electrode locations on the scalp and its features were extracted and analysed using common spatial pattern (CSP) filter. These features were subsequently fed into a support vector machine (SVM) classifier for classification. This analysis aimed to perform a binary classification, predicting whether the subject was performing one task or the other. Results from an extensive analysis showed a mean classification accuracy of 88.9% for VIm task and 91.1% for VInt task. This study clearly shows that VIm can be classified with ease and is a viable paradigm to integrate in BCIs. Such systems are not only useful for people with speech problems, but in general for people who use BCI systems to help them out in their everyday life, giving them another dimension of system control.
Alexander Borch Kristensen, Younes Subhi, Sadasivan Puthusserypady

2080 related Products with: Vocal Imagery vs Intention: Viability of Vocal Based EEG-BCI Paradigms.

One 96-Well Microplate Ki 125 ml 100ug Lyophilized250ulOne 96-Well Microplate Ki100tests 1000 ml 100 ml100ugOne 96-Well Microplate Ki1000 assays

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#32745917   2020/07/24 To Up

A simple and robust method for radiochemical separation of no-carrier-added Cu produced in a research reactor for radiopharmaceutical preparation.

Copper-64 is an excellent theranostic radiometal that is gaining renewed attention of the clinical community in the recent times. In order to meet the increasing demand of this radiometal, we have demonstrated the viability of its production via Zn (n,p) Cu reaction in a nuclear reactor. A semi-automated radiochemical separation module based on selective extraction of Cu as dithizonate complex was developed. The maximum available activity at the end of irradiation was ~ 700 MBq. The overall yield of Cu after the separation process was >85% and it could be obtained with ~12 GBq/μg specific activity, >99.9% radionuclidic purity and >98% radiochemical purity. The separated Cu could be utilized for preparation of a wide variety of radiopharmaceuticals.
Rubel Chakravarty, Ardhi Rajeswari, Priyalata Shetty, K C Jagadeesan, Ramu Ram, Sachin Jadhav, Haladhar Dev Sarma, Ashutosh Dash, Sudipta Chakraborty

1858 related Products with: A simple and robust method for radiochemical separation of no-carrier-added Cu produced in a research reactor for radiopharmaceutical preparation.

100Tests96 Tests25 µg1 kitcase 1 G100μg24 tests1 LITRE 100ul

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#32745911   2020/07/31 To Up

Ursolic acid protects chondrocytes, exhibits anti-inflammatory properties via regulation of the NF-κB/NLRP3 inflammasome pathway and ameliorates osteoarthritis.

Inflammation and poor viability of chondrocytes result in the degradation of cartilage as osteoarthritis (OA) progresses. The purpose of the present study was to investigate whether ursolic acid (UA) can protect chondrocytes and alleviate OA. Following stimulation with tumor necrosis factor-α (TNF-α), 5 μM UA displayed no cytotoxicity and reversed the up-regulation of the inflammatory factors MMP13, IL-1β, IL-6 and PTGS2, and down-regulation of the cartilaginous genes/proteins type II collagen and Aggrecan. RNA sequencing identified 533 common deferentially expressed genes (DEGs) of which TNF, PI3K-AKT, NOD-like receptor, cytokine receptor interaction and NF-κB pathways were of potential importance. Further notable DEGs in the most-highly expressed 10 pathways contributed to maintenance of cartilaginous ECM homeostasis and were involved in an inflammatory response. The expression of these most-enriched DEGs was reversed by UA following stimulation with TNF-α. Additional investigation demonstrated that treatment with UA inhibited TNF-α-induced nuclear translocation of p65 and phosphorylation of IκBα and AKT, and reversed TNF-α-induced up-regulation of P20, ACS and NLRP3. Furthermore, rat anterior cruciate ligament transection (ACLT) induced-OA was ameliorated by treatment with UA. In conclusion, these results suggest that UA activates chondrocytes through the NF-κB/NLRP3 inflammasome pathway, thus preventing cartilage degeneration in osteoarthritis.
Chunli Wang, Yan Gao, Zike Zhang, Cheng Chen, Qingjia Chi, Kang Xu, Li Yang

1337 related Products with: Ursolic acid protects chondrocytes, exhibits anti-inflammatory properties via regulation of the NF-κB/NLRP3 inflammasome pathway and ameliorates osteoarthritis.

100ug Lyophilized100ug Lyophilized100 ug/vial100ul1000 100ug Lyophilized100 μg10 mg100ug Lyophilized100ug Lyophilized100.00 ul100ug Lyophilized

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#32745772   2020/07/31 To Up

Melaleuca leucadendron (L.) L. flower extract exhibits antioxidant and photoprotective activities in human keratinocytes exposed to ultraviolet B radiation.

Recently, there has been a demand for the replacement of chemical sunscreens with natural compounds that could prevent or restore UV-induced skin damage. Here, we investigated the photoprotective influence of the Melaleuca leucadendron ethanolic flower extract (EEMec) on factors involved in cellular and molecular UVB-induced oxidative stress in human skin keratinocytes (HaCaT). The phytochemical constituents, antioxidant potential by DPPH assay, content of total phenolic and flavonoid compounds in EEMec were evaluated. HaCaT cells were treated with EEMec followed by irradiation with UVB. CAT activity; GSH and ROS levels; and SOD1, GPx, CAT and COX-2 expression assays were employed to verify the oxidative stress, as well as EEMec effect on transmembrane transport, and pro-inflammatory and pro-apoptotic protein expression. EEMec reverted the viability loss of HaCaT cells after irradiation with UVB, exhibited significant antioxidant capacity and free radical scavenging activity in vitro, inhibited COX-2 expression and ensure protection of DNA-damage. EEMec shown a great photoprotective property to prevent keratinocytes damage induced by UV radiation and, thus a candidate potential to application as an adjuvant in sunscreen formulations as a strategy to reduce risk of sunburn and prevent skin diseases associated with UV-induced inflammation and cancer.
Luan Silvestro Bianchini Silva, Fernanda Barçante Perasoli, Karen Vitor Carvalho, Karla Murata Vieira, Miriam Teresa Paz Lopes, Gustavo Henrique Bianco de Souza, Orlando David Henrique Dos Santos, Kátia Michelle Freitas

1018 related Products with: Melaleuca leucadendron (L.) L. flower extract exhibits antioxidant and photoprotective activities in human keratinocytes exposed to ultraviolet B radiation.

100 μg2ug100.00 ug100 μg500 96 wells (1 kit)100 μg100 μg100 μg100 μg1

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#32745672   2020/06/26 To Up

A hidden risk: Survival and resuscitation of Escherichia coli O157:H7 in the viable but nonculturable state after boiling or microwaving.

We report the existence and resuscitation of viable but nonculturable (VBNC) Escherichia coli O157:H7 cells in drinking water induced by the common point-of-use disinfection treatments of boiling or microwaving. Tap water and saline samples containing E. coli O157:H7 culturable cells from a bovine isolate or two clinical isolates were boiled (1, 10, or 15 min) on a hot plate or microwaved (1.5 min) to reach boiling. No culturable E. coli O157:H7 cells were observed in the treated samples using conventional plating methods. In samples boiled for 1 or 10 min, two viability assays separately detected that 2-5.5% of the cells retained an intact membrane, while 28 to 87 cells out of the initial 10 cells retained both measurable intracellular esterase activity and membrane integrity. In samples boiled for 15 min, no viable cells were detected. The microwaved samples contained 6-10% of cells with an intact membrane, while 21 to 108 cells out of the initial 10 cells retained both membrane integrity and esterase activity. The number of viable cells retaining both metabolic activity and membrane integrity were consistent in all samples, supporting the survival of a small number of E. coli O157:H7 cells in the VBNC state after boiling for 1 or 10 min or microwaving. Furthermore, the VBNC E. coli O157:H7 cells regained growth at 37 °C in culture media containing autoinducers produced by common non-pathogenic E. coli, commonly present in the human intestine, and norepinephrine. The resuscitated cells were culturable on conventional plates and expressed mRNA encoding the E. coli O157 lipopolysaccharide gene (rfbE) and the H7 flagellin gene (fliC). This study highlights potential concerns for public health risk management of VBNC E. coli O157:H7 in drinking water disinfected by heat treatment at point-of-use. The public health significance of these concerns warrants further investigation.
Yanming Liu, Teresa Kumblathan, Gursharan K Uppal, Angela Zhou, Birget Moe, Steve E Hrudey, Xing-Fang Li

1530 related Products with: A hidden risk: Survival and resuscitation of Escherichia coli O157:H7 in the viable but nonculturable state after boiling or microwaving.

10.1 mg200 100

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#32745656   2020/07/31 To Up

Valproate improves middle cerebral artery occlusion-induced ischemic cerebral disorders in mice and oxygen-glucose deprivation-induced injuries in microglia by modulating RMRP/PI3K/Akt axis.

Valproate (VPA) is capable of attenuating ischemic stroke (IS)-related disorders in brain tissues. Long non-coding RNAs (lncRNAs) are involved in the progression of IS. In the current study, the role of lncRNA RMRP in the protective effects of VPA against IS was explored. Mice were subjected to middle cerebral artery occlusion (MCAO) model to induce IS injures and then were administrated with VPA. The effects of VPA on infarction area and apoptosis in brain tissues, and the RMRP-regulated PI3K/Akt signaling activity were detected. Thereafter, oxygen-glucose deprivation (OGD) BV-2 cells were used as the in vitro model to further explore the mechanism underlying VPA function. The administration of VPA reduced infarction area and suppressed apoptosis in brain tissues of MCAO mice. VPA also inhibited RMRP expression and activated PI3K/Akt signaling. In OGD BV-2 cells, the treatment of VPA increased viability and attenuated apoptosis, which was associated with the inhibition of RMRP and the activation of PI3K/Akt pathway. Moreover, the induced expression of RMRP blocked the anti-OGD function of VPA, indicating the key role of RMRP inhibition in the effects of VPA on nerve system. Collectively, VPA attenuated MCAO/OGD-induced disorders in mice and microglia. The effects were dependent on the inhibition of RMRP, which subsequently induced the activation of PI3K/Akt signaling.
Xuetang Li, Yuling Sui

2229 related Products with: Valproate improves middle cerebral artery occlusion-induced ischemic cerebral disorders in mice and oxygen-glucose deprivation-induced injuries in microglia by modulating RMRP/PI3K/Akt axis.

400 ug100 ul 100ul96 assays1 mg100ug50 ul1 mg100ug96T50 ul5ug

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#32745469   2020/07/31 To Up

miR-188-5p inhibits apoptosis of neuronal cells during oxygen-glucose deprivation (OGD)-induced stroke by suppressing PTEN.

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Lijing Li, Penghua Cui, Huimin Ge, Yanjing Shi, Xiaoguang Wu, Zhongfan Ruan

2071 related Products with: miR-188-5p inhibits apoptosis of neuronal cells during oxygen-glucose deprivation (OGD)-induced stroke by suppressing PTEN.

96 assays 1 kit(s) 4 Membranes/Box50ml75 x 50ul/Unit200 200 1mg1.00 flask100 assays100 µg

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#32745459   2020/07/31 To Up

Proactive Neonatal Treatment at 22 Weeks of Gestation: A Systematic Review and Meta-Analysis.

The objective of the present study was to provide a systemic review and meta-analysis to quantify prognosis and identify factors associated with variations in reported mortality estimates among infants born at 22 weeks of gestation and provided proactive treatment (resuscitation and intensive care).
Carl H Backes, Brian K Rivera, Leanne Pavlek, Lindsey J Beer, Molly K Ball, Eli T Zettler, Charles V Smith, Jeffrey A Bridge, Edward F Bell, Heather A Frey

2072 related Products with: Proactive Neonatal Treatment at 22 Weeks of Gestation: A Systematic Review and Meta-Analysis.

100 mg5 mg10 mg1 module25 mg100 μg100ul3reactions1 module 100ul100ug100ul

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