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#39278904 
2024/09/15
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Multiomics integrated analysis and experimental validation identify TLR4 and ALOX5 as oxidative stress-related biomarkers in intracranial aneurysms.
Intracranial aneurysm (IA) is a severe cerebrovascular disease, and effective gene therapy and drug interventions for its treatment are still lacking. Oxidative stress (OS) is closely associated with the IA, but the key regulatory genes involved are still unclear. Through multiomics analysis and experimental validation, we identified two diagnostic markers for IA associated with OS.Lvyin Luo, Xinlong Ma, Debin Kong, Yuxiang Dai, Tao Li, Han Yu, Jingzheng Liu, Maogui Li, Yangyang Xu, Guo Xiang, Zhimin Zhao, Weiying Zhong, Donghai Wang, Yunyan Wang
1280 related Products with: Multiomics integrated analysis and experimental validation identify TLR4 and ALOX5 as oxidative stress-related biomarkers in intracranial aneurysms.
12.5 mg20 100ug48 assays 10 mg100ul 5 G50 ug 100 μg
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#39278098 2024/09/10
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Down-regulation of CORO1C mediated by lncMALAT1/miR-133a-3p axis contributes to trophoblast dysfunction and preeclampsia.
Placental trophoblast dysfunction has been proved to be closely related to the pathogenesis of preeclampsia. Coronaryxin-like actin-binding protein 1C (CORO1C) plays an important role in cell proliferation, apoptosis, invasion, and signal transduction, but its involvement in trophoblast dysfunction and preeclampsia remains uncertain.Hanhui Nie, Xiufang Wang, Xiaohui Dong, Yiling Wei, Jiachun Wei, Ka Cheuk Yip, Qiao Zhang, Ruiman Li
1527 related Products with: Down-regulation of CORO1C mediated by lncMALAT1/miR-133a-3p axis contributes to trophoblast dysfunction and preeclampsia.
100 mg96 wells100ul1 module1000 tests1 module0.5mg 100 G1 kit(96 Wells)1 ml
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#39277758 2024/09/14
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Identification and function characterization of NcAP2XII-4 in Neospora caninum.
Neospora caninum is a protozoan parasite in the Apicomplexa controlled by complex signaling pathways. Transcriptional control, an important way to regulate gene expression, has been almost absent in the N. caninum life process. However, to date, research on the transcriptional regulation of the AP2 family factors in N. caninum has been extremely limited. A prior study demonstrated that removing rhoptry protein 5 (ROP5), a significant virulence factor, resulted in abnormal expression levels of predicted NcAP2XII-4 in N. caninum, suggesting that the factor may regulate the function of ROP5. This study aimed to identify NcAP2XII-4 and its function in transcriptional regulation.Huizhu Nan, Xin Lu, Chao Zhang, Xin Yang, Zhu Ying, Lei Ma
2388 related Products with: Identification and function characterization of NcAP2XII-4 in Neospora caninum.
25 G4/120 Packing /sleeve/bo5 g400Tests10mg50 mg 5 G5mg4/120 Packing /sleeve/bo1 g 5 G4/120 Packing /sleeve/bo
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#39276829 2024/09/12
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Antibiotic residues in the cirata reservoir, Indonesia and their effect on ecology and the selection for antibiotic-resistant bacteria.
Antibiotic residues, their mixture toxicity, and the potential selection for antibiotic-resistant bacteria could pose a problem for water use and the ecosystem of reservoirs. This study aims to provide a comprehensive understanding of the occurrence, concentration, distribution, and ecological risks associated with various antibiotics in the Cirata reservoir, Indonesia. In our water and sediment samples, we detected 24 out of the 65 antibiotic residues analyzed, revealing a diverse range of antibiotic classes present. Notably, sulphonamides, diaminopyrimidine, and lincosamides were frequently found in the water, while the sediment predominantly contained tetracyclines and fluoroquinolones. Most antibiotic classes reached their highest concentrations in the water during the dry season. However, fluoroquinolones and tetracyclines showed their highest concentrations in the water during the wet season. Ecotoxicological risk assessments indicated that the impact of most antibiotic residues on aquatic organisms was negligible, except for fluoroquinolones. Looking at the impact on cyanobacteria, however, varying risks were indicated, ranging from medium to critical, with antibiotics like sulfamethoxazole, ciprofloxacin, norfloxacin, and lincomycin posing substantial threats. Among these, ciprofloxacin emerged as the antibiotic with the strongest risk. Furthermore, fluoroquinolones may have the potential to contribute to the selection of antibiotic-resistant bacteria. The presence of mixtures of antibiotic residues during the wet season significantly impacted species loss, with Potentially Affected Fraction of Species (msPAF) values exceeding 0.75 in almost 90% of locations. However, the impact of mixtures of antibiotic residues in sediment remained consistently low across all locations and seasons. Based on their occurrences and associated risks, 12 priority antibiotic residues were identified for monitoring in the reservoir and its tributaries. Moreover, the study suggests that river inflow serves as the most significant source of antibiotic residues in the reservoir. Further investigations into the relative share attribution of antibiotic sources in the reservoir is recommended to help identify effective interventions.Miranti Ariyani, Larissa J M Jansen, Paula Balzer-Rutgers, Nynke Hofstra, Pieter van Oel, Milou G M van de Schans
1853 related Products with: Antibiotic residues in the cirata reservoir, Indonesia and their effect on ecology and the selection for antibiotic-resistant bacteria.
11
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#39274939 2024/08/29
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Dammarane-Type 3,4--Triterpenoid from Silver Birch ( Roth) Buds Induces Melanoma Cell Death by Promotion of Apoptosis and Autophagy.
Despite unquestionable advances in therapy, melanoma is still characterized by a high mortality rate. For years, high expectations have been raised by compounds of natural origin as a component of pharmacotherapy, particularly by triterpenes found in the bark of birch trees. In this study, 3,4--dammara-4(29),20(21),24(25)-trien-3-oic acid (SDT) was isolated from buds of silver birch and its mechanisms of cell death induction, including apoptosis and autophagy, were determined. Cytotoxicity of SDT was evaluated by the cell viability test and clonogenic assay, whereas induction of apoptosis and autophagy was determined by annexin V staining and Western blot. The results revealed dose- and time-dependent reductions in viability of melanoma cells. Treatment of cells for 48 h led to an increase in the percentage of annexin V-positive cells, activation of caspase-8, caspase-9, and caspase-3, and cleavage of PARP, confirming apoptosis. Simultaneously, it was found that SDT increased the level of autophagy marker LC3-II and initiator of autophagy beclin-1. Pretreatment of cells with caspase-3 inhibitor or autophagy inhibitor significantly reduced the cytotoxicity of SDT and revealed that both apoptosis and autophagy contribute to a decrease in cell viability. These findings suggest that 3,4--dammaranes may become a promising group of natural compounds for searching for anti-melanoma agents.Lukasz Szoka, Marcin Stocki, Valery Isidorov
2138 related Products with: Dammarane-Type 3,4--Triterpenoid from Silver Birch ( Roth) Buds Induces Melanoma Cell Death by Promotion of Apoptosis and Autophagy.
0.5 mg100ug Lyophilized0.5 mg1 kit100ug Lyophilized100ug Lyophilized1 kit100 Tests100ug Lyophilized
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#39274878 2024/08/26
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Dehydroevodiamine Alleviates Ulcerative Colitis by Inhibiting the PI3K/AKT/NF-κB Signaling Pathway via Targeting AKT1 and Regulating Gut Microbes and Serum Metabolism.
Ulcerative colitis (UC) is a typical inflammatory bowel disease (IBD), impairing the quality of life of patients. Dehydroevodiamine (DHE) is an active alkaloid isolated from that exerts significant anti-inflammatory effects in gastrointestinal diseases. However, the effect and mechanisms of DHE on UC remain unclear. We performed a DSS-induced experimental UC rat model to reveal the efficacy and potential mechanisms of DHE on UC. HE and AB-PAS staining were used for the evaluation of pathologies, and 16S rRNA sequencing was used to detect changes in gut microbes. Metabolomics was used to detect changes in serum metabolites. Network pharmacology and transcriptomics were conducted to reveal the underlying mechanisms of DHE for UC. HuProt proteome microarrays, molecular docking, and SPR were used to reveal the targets of action of DHE. WB, RT-qPCR, and IHC were used to assess the action effects of DHE. DHE demonstrated significant alleviation of DSS-induced colitis symptoms in rats by suppressing inflammatory and oxidative stress responses, amending colonic barrier injury, and inhibiting apoptosis. In terms of gut microbial modulation, DHE decreased the abundance of , , , , and and increased the abundance of , , and . Moreover, metabolomics suggested that the regulation of DHE in DSS-induced UC rats mainly involved aminoacyl-tRNA biosynthesis, vitamin B6 metabolism, phenylalanine, tyrosine, and so on. Mechanically, DHE alleviated UC in rats by targeting AKT1, thereby inhibiting the PI3K/AKT/NF-κB signaling pathway.Xiao Ma, Qichao Hu, Tao Jiang, Yuan Chen, Wenwen Zhang, Pan Gao, Jinhao Zeng, Thomas Efferth
2878 related Products with: Dehydroevodiamine Alleviates Ulcerative Colitis by Inhibiting the PI3K/AKT/NF-κB Signaling Pathway via Targeting AKT1 and Regulating Gut Microbes and Serum Metabolism.
96T1,000 tests1 mg100ul2 Pieces/Box14 inhibitors2 Pieces/Box100 mg1000 tests10 mg1 ml
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#39273931 2024/09/01
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A Comprehensive Review of Climate Change and Plant Diseases in Brazil.
Analyzing the impacts of climate change on phytosanitary problems in Brazil is crucial due to the country's special role in global food security as one of the largest producers of essential commodities. This review focuses on the effects of climate change on plant diseases and discusses its main challenges in light of Brazil's diverse agricultural landscape. To assess the risk of diseases caused by fungi, bacteria, viruses, oomycetes, nematodes, and spiroplasms, we surveyed 304 pathosystems across 32 crops of economic importance from 2005 to 2022. Results show that diseases caused by fungi account for 79% of the pathosystems evaluated. Predicting the occurrence of diseases in a changing climate is a complex challenge, and the continuity of this work is strategic for Brazil's agricultural defense. The future risk scenarios analyzed here aim to help guide disease mitigation for cropping systems. Despite substantial progress and ongoing efforts, further research will be needed to effectively prevent economic and environmental damage.Francislene Angelotti, Emília Hamada, Wagner Bettiol
1367 related Products with: A Comprehensive Review of Climate Change and Plant Diseases in Brazil.
500 tests500 tests500 tests4/120 Packing /sleeve/bo500 tests4/120 Packing /sleeve/bo500 tests500 tests500 tests
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#39273074 2024/09/08
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: Unveiling the Function of a Novel Gene Associated with Hereditary Myopathy.
was identified as a novel candidate gene for autosomal dominant centronuclear myopathy-4 (CNM4) approximately ten years ago. However, to date, only one family has been described, and the function of CCDC78 remains unclear. Here, we analyze for the first time a family harboring a nonsense mutation to better understand the role of CCDC78 in muscle.Diego Lopergolo, Gian Nicola Gallus, Giuseppe Pieraccini, Francesca Boscaro, Gianna Berti, Giovanni Serni, Nila Volpi, Patrizia Formichi, Silvia Bianchi, Denise Cassandrini, Vincenzo Sorrentino, Daniela Rossi, Filippo Maria Santorelli, Nicola De Stefano, Alessandro Malandrini