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Search results for: Anti-human Apolipoprotein-H (β2-Glycoprotein-I ) Antibody

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#35740387   2022/06/09 To Up

Antihuman Endogenous Retrovirus Immune Response and Adaptive Dysfunction in Autism.

ASD is a neurodevelopmental disorder of unknown aetiology but with a known contribution of pathogenic immune-mediated mechanisms. HERVs are associated with several neuropsychiatric diseases, including ASD. We studied anti-HERV-W, -K and -H-env immune profiles in ASD children to analyse differences between their respective mothers and child/mother control pairs and possible correlations to ASD severity and loss of adaptive abilities. Of the 84 studied individuals, 42 children (23 ASD and 19 neurotypical) and their paired mothers underwent clinical and neuropsychological evaluations. ASD severity was analysed with standardised tests. Adaptive functioning was studied with ABAS-II and GAC index. Plasma anti-env responses of HERV-K, -H and -W were tested with indirect ELISA. ASD and neurotypical children did not differ in age, gender, comorbidities and anti-HERV responses. In children with ASD, anti-HERV levels were not correlated to ASD severity, while a significant inverse correlation was found between anti-HERV-W-248-262 levels and adaptive/social abilities. Upregulation of anti-HERV-W response correlates to dysfunctional social and adaptive competences in ASD but not in controls, suggesting anti-HERV response plays a role in the appearance of peculiar ASD symptoms.
Alessandra Carta, Maria Antonietta Manca, Chiara Scoppola, Elena Rita Simula, Marta Noli, Stefano Ruberto, Marta Conti, Ignazio Roberto Zarbo, Roberto Antonucci, Leonardo A Sechi, Stefano Sotgiu

2448 related Products with: Antihuman Endogenous Retrovirus Immune Response and Adaptive Dysfunction in Autism.

96T96T 100 UG100 μg2 100 μg100 μg100 mg100ug Lyophilized

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#35675509   2022/06/08 To Up

Antibody-Invertase Fusion Protein Enables Quantitative Detection of SARS-CoV-2 Antibodies Using Widely Available Glucometers.

Rapid diagnostics that can accurately inform patients of disease risk and protection are critical to mitigating the spread of the current COVID-19 pandemic and future infectious disease outbreaks. To be effective, such diagnostics must rely on simple, cost-effective, and widely available equipment and should be compatible with existing telehealth infrastructure to facilitate data access and remote care. Commercial glucometers are an established detection technology that can overcome the cost, time, and trained personnel requirements of current benchtop-based antibody serology assays when paired with reporter molecules that catalyze glucose conversion. To this end, we developed an enzymatic reporter that, when bound to disease-specific patient antibodies, produces glucose in proportion to the level of antibodies present in the patient sample. Although a straightforward concept, the coupling of enzymatic reporters to secondary antibodies or antigens often results in low yields, indeterminant stoichiometry, reduced target binding, and poor catalytic efficiency. Our enzymatic reporter is a novel fusion protein that comprises an antihuman immunoglobulin G (IgG) antibody genetically fused to two invertase molecules. The resulting fusion protein retains the binding affinity and catalytic activity of the constituent proteins and serves as an accurate reporter for immunoassays. Using this fusion, we demonstrate quantitative glucometer-based measurement of anti-SARS-CoV-2 spike protein antibodies in blinded clinical sample training sets. Our results demonstrate the ability to detect SARS-CoV-2-specific IgGs in patient serum with precise agreement to benchmark commercial immunoassays. Because our fusion protein binds all human IgG isotypes, it represents a versatile tool for detection of disease-specific antibodies in a broad range of biomedical applications.
Elissa K Leonard, Miguel Aller Pellitero, Boris Juelg, Jamie B Spangler, Netzahualcóyotl Arroyo-Currás

1745 related Products with: Antibody-Invertase Fusion Protein Enables Quantitative Detection of SARS-CoV-2 Antibodies Using Widely Available Glucometers.

1 mg100ug Lyophilized25mg200ul200ul100ug Lyophilized250mg100 0.1 mg250ul100ug Lyophilized

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#35584496   // To Up

Expression of endoglin, CD105, in conjunctival melanocytic nevi: Is it suspicious like in thyroidology? Oculi plus vident quam oculus?

The aim of this study was to evaluate the expression of endoglin and its correlation with histopathological and clinical findings in conjunctival nevi.
Dzenana Detanac, Snezana Jancic, Demet Sengul, Ilker Sengul, Dzemail Detanac

2754 related Products with: Expression of endoglin, CD105, in conjunctival melanocytic nevi: Is it suspicious like in thyroidology? Oculi plus vident quam oculus?

100 ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100 ug100ug0.1ml100 μg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#35520371   2022/04/28 To Up

Retiform Purpura of the Lower Limbs Associated with Levamisole-Adulterated Cocaine.

A young man was treated in hospital for sepsis, disseminated intravascular coagulation and multi-organ failure. He was a regular intranasal cocaine user up to 1 day prior to symptom onset. Clinical examination revealed extensive retiform purpura affecting both his lower limbs. Skin biopsy revealed widespread thrombosis in the small- and medium-sized vessels of the mid dermis and the subcutaneous fat with surrounding leucocytoclasis. There was also extensive ischaemic necrosis of the upper reticular and papillary dermis and focal ischaemic necrosis of the epidermis. These findings were in keeping with a thrombotic vasculopathy with associated cutaneous ischaemic necrosis, likely associated with levamisole-adulterated cocaine (LAC). An autoimmune screen showed extremely raised levels of anti-B2-glycoprotein IgM, IgG and anti-cardiolipin IgG antibodies, usually seen in antiphospholipid syndrome (APS). The literature describes how APS could be secondary to various underlying conditions, including LAC, and that levamisole toxicity may mimic APS and hence be missed.
Stephanie Farrugia, Daniel Micallef, David Pisani, Alexandra Betts, Sandro Vella, Michael J Boffa

1548 related Products with: Retiform Purpura of the Lower Limbs Associated with Levamisole-Adulterated Cocaine.

101 kg100 IU0.1 mg500 Units12

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#35503752   2022/05/03 To Up

AbSE Workflow: Rapid Identification of the Coding Sequence and Linear Epitope of the Monoclonal Antibody at the Single-cell Level.

Monoclonal antibody (mAb) has been widely used in immunity research and disease diagnosis and therapy. Antibody sequence and epitope are the prerequisites and basis of mAb applications, which determine the properties of antibodies and make the preparation of antibody-based molecules controllable and reliable. Here, we present the antibody sequence and epitope identification (AbSE) workflow, a time-saving and cost-effective route for rapid determination of antibody sequence and linear epitope of mAb even at the single-cell level. The feasibility and accuracy of the AbSE workflow were demonstrated through the identification and validation of the coding sequence and epitope of antihuman serum albumin (antiHSA) mAb. It can be inferred that the AbSE workflow is a powerful and universal approach for paired antibody-epitope sequence identification. It may characterize antibodies not only on a single hybridoma cell but also on any other antibody-secreting cells.
Lerong Ma, HongSheng Ouyang, Ang Su, Yuanzhu Zhang, Daxin Pang, Tao Zhang, Ruize Sun, Wentao Wang, Zicong Xie, Dongmei Lv

1764 related Products with: AbSE Workflow: Rapid Identification of the Coding Sequence and Linear Epitope of the Monoclonal Antibody at the Single-cell Level.

200 units200 units

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#35482487   2022/04/28 To Up

Too Sensitive or Just Right?

To compare the performance of the rabbit monoclonal antihuman CD246 antibody (D5F3 clone) with the established ALK1 clone for immunohistochemical assessment of anaplastic large cell lymphoma (ALCL).
Dana Martin, Steven C Smith, Alden Chesney, Colleen Jackson-Cook, Raghavendra Pillappa

2462 related Products with: Too Sensitive or Just Right?

5 mg100ug Lyophilized1 module1001 module100ug100 ug250ul

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#35451149   2022/04/22 To Up

Noninvasive isolation of transcervical trophoblast cells for fetal identification.

To identify trophoblastic cells retrieved from the cervix at a gestational age (GA) of 5-9 weeks by a noninvasive modality in fetuses.
Yi Gao, Liping Ruan, Leilei Cao, Gaochuan Lu, Qin Hong, Qian Zhang, Yincheng Teng, Zhijie Wang

1579 related Products with: Noninvasive isolation of transcervical trophoblast cells for fetal identification.

0.1ml (1mg/ml)4 X 250 ml. 100 ml10 ml1.00 flask 5 lt10 assays1.0 mg5L 1 kit(s) 10ml

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#35155848   2022/02/07 To Up

Occurrence of Donor-Specific Antibodies After COVID-19 in Kidney Transplant Recipients Is Low Despite Immunosuppression Modulation.

Decreased immunosuppression has been proposed for kidney transplant recipients infected with coronavirus disease 2019 (COVID-19), but the impact on the alloreactive immune response during and after infection has been poorly investigated. We evaluated the occurrence of antihuman leukocyte antigen (HLA) donor-specific antibodies (DSAs) (post-COVID-19) and rejection episodes after COVID-19 with particular focus on immunosuppression modulation.
Christophe Masset, Gabriela Gautier-Vargas, Diego Cantarovich, Simon Ville, Jacques Dantal, Florent Delbos, Alexandre Walencik, Clarisse Kerleau, Maryvonne Hourmant, Claire Garandeau, Aurélie Meurette, Magali Giral, Ilies Benotmane, Sophie Caillard, Gilles Blancho

2703 related Products with: Occurrence of Donor-Specific Antibodies After COVID-19 in Kidney Transplant Recipients Is Low Despite Immunosuppression Modulation.

4 Membranes/Box2 Pieces/Box100 μg4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box2 Pieces/Box4 Membranes/Box100 μg4 Arrays/Slide

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#35048001   2021/09/10 To Up

Expression of Interleukin-1ß and Interleukin-8 in Oral Potentially Malignant Disorders and Carcinomas.

To evaluate interleukin-1ß (IL-1ß) and interleukin-8 (IL-8) epithelial expressions in potentially malignant disorders of the oral mucosa as malignant predictive markers. About 55 tissues embedded in paraffin, comprising 15 oral lichen planus (OLP) lesions, 15 leukoplakias, 15 oral squamous cell carcinomas (OSCC), and 10 samples of normal oral mucosa were included in the study. IL-1ß and 8 expressions were assessed by immunohistochemistry using antibodies antihuman IL-1ß human (sc-7884, Santa Cruz® H-153) and antihuman IL-8 (ab7747, abcam®). The number of positive cells was compared using Student's -test. Any -value < 0.05 was considered statistically significant. Nuclear and cytoplasmatic keratinocyte staining were positive for both cytokines in all study groups. However, a statistically significant decrease was observed within all cases compared to normal mucosa, both staining for IL-1β and 8. Moreover, IL-8 showed significant differences between OLP and leukoplakia, and when compared to OSCC. Oral epithelial expression of IL-1β and 8 seems to decrease when the malignant transformation of the oral mucosa increases.
Jeaneth Lopez-Labady, Ronell Bologna-Molina, Mariana Villarroel-Dorrego

2252 related Products with: Expression of Interleukin-1ß and Interleukin-8 in Oral Potentially Malignant Disorders and Carcinomas.

10 96 wells (1 kit)10 1 kit(96 Wells)96 wells (1 kit)500 96T100.00 ug 0.1 mg 2 96 wells (1 kit)2

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