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Search results for: BZW1

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#33393590   2021/01/04 To Up

LncRNA NEAT1 promotes glioma cancer progression via regulation of miR-98-5p/BZW1.

Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas.
Yabin Li, Xirui Wang, Zhihuang Zhao, Jinxing Shang, Gang Li, Ruijian Zhang

2044 related Products with: LncRNA NEAT1 promotes glioma cancer progression via regulation of miR-98-5p/BZW1.



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#32855654   2020/08/13 To Up

Integrated Genome-Wide Methylation and Expression Analyses Reveal Key Regulators in Osteosarcoma.

Osteosarcoma (OS) is one of the most common types of primary bone tumors in early adolescence with unsatisfied prognosis. Aberrant DNA methylation had been demonstrated to be related to tumorigenesis and progression of multiple cancers and could serve as the potential biomarkers for the prognosis of human cancers. In conclusion, this study identified 18 downregulated hypomethylation genes and 52 upregulated hypomethylation genes in OS by integrating the analysis the GSE97529 and GSE42572 datasets. Bioinformatics analysis revealed that OS-specific methylated genes were involved in regulating multiple biological processes, including chemical synaptic transmission, transcription, response to drug, and regulating immune response. KEGG pathway analysis showed that OS-specific methylated genes were associated with the regulation of Hippo, cAMP calcium, MAPK, and Wnt signaling pathways. By analyzing R2 datasets, this study showed that the dysregulation of these OS-specific methylated genes was associated with the metastasis-free survival time in patients with OS, including CBLN4, ANKMY1, BZW1, KRTCAP3, GZMB, KRTDAP, LY9, PFKFB2, PTPN22, and CLDN7. This study provided a better understanding of the molecular mechanisms underlying the progression and OS and novel biomarkers for the prognosis of OS.
Fei Wang, Guoqing Qin, Junzhi Liu, Xiunan Wang, Baoguo Ye

1545 related Products with: Integrated Genome-Wide Methylation and Expression Analyses Reveal Key Regulators in Osteosarcoma.

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#31949677   2018/12/01 To Up

MicroRNA-129-3p functions as a tumor suppressor in serous ovarian cancer by targeting BZW1.

The aberrant expression of microRNAs (miRNAs) underlies a series of human diseases, including ovarian cancers. In our previous study, we found that miR-129-1-3p and miR-129-2-3p levels were significantly decreased in serous ovarian cancer via a microarray and quantitative PCR. In this study, we investigated the pathological role of miR-129-3p in an ovarian cancer cell line, SKOV3 cells. The results demonstrated that miR-129-3p overexpression distinctively inhibited the proliferation, migration, and invasion of ovarian cancer cells. The regulator of cell cycling, BZW1 (basic leucine zipper and W2 domains 1), was validated as a novel direct target of miR-129-3p. Specifically, miR-129-3p bound directly to the 3' untranslated region of BZW1 and suppressed its expression. Our results indicate that miR-129-3p serves as a tumor suppressor by targeting BZW1 in ovarian cancer cells and highlight that the restoration of miR-129-3p might be a novel therapeutic strategy for ovarian cancer.
Fei Liu, Hongxi Zhao, Li Gong, Li Yao, Yanhong Li, Wei Zhang

1936 related Products with: MicroRNA-129-3p functions as a tumor suppressor in serous ovarian cancer by targeting BZW1.



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#31601833   2019/10/10 To Up

Overexpression of BZW1 is an independent poor prognosis marker and its down-regulation suppresses lung adenocarcinoma metastasis.

The basic leucine zipper and the W2 domain-containing protein 1 (BZW1) plays a key role in the cell cycle and transcriptionally control the histone H4 gene during G1/S phase. Since cellular proliferation rates are frequently dysregulated in human cancers, we identified the characteristics of BZW1 in cancer cells and analyzed its prognostic value in lung cancer patients. By searching public databases, we found that high BZW1 expression was significantly correlated with poor survival rate in non-small cell lung cancer (NSCLC), especially in lung adenocarcinoma. Similar trends were also shown in an array comprising NSCLC patient tissue. Knockdown of BZW1 inhibited cell metastatic ability, but did not affect the cell proliferation rate of NSCLC cells. From transcriptomics data mining, we found that coordination between BZW1 and EGFR overexpression was correlated with a worse outcome for lung cancer patients. In summary, BZW1 expression serves as an independent prognostic factor of NSCLC, especially in lung adenocarcinoma. Overexpression of BZW1 in lung cancer cells revealed a novel pathway underlying the induction of lung cancer metastasis.
Jean Chiou, Yu-Chan Chang, Yi-Hua Jan, Hsing-Fang Tsai, Chih-Jen Yang, Ming-Shyan Huang, Yung-Luen Yu, Michael Hsiao

1871 related Products with: Overexpression of BZW1 is an independent poor prognosis marker and its down-regulation suppresses lung adenocarcinoma metastasis.

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#24797264   2014/05/05 To Up

Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.

Post-translational modification by SUMO is a highly conserved pathway in eukaryotes that plays very important regulatory roles in many cellular processes. Deregulation of the SUMO pathway contributes to the development and progression of many diseases including cancer. Therefore, identifying additional SUMO substrates and studying how their cellular and biological functions are regulated by sumoylation should provide new insights. Our studies showed that sumoylation activity was significant in Xenopus egg extracts, and that a high level of sumoylation was associated with sperm chromatin when SUMO was incubated with Xenopus egg extracts. By isolating SUMO-conjugated substrates using His-tagged SUMO1 or SUMO2 proteins under denaturing conditions, we identified 346 proteins by mass spectrometry analysis that were not present in control pull-downs. Among them, 167 proteins were identified from interphase egg extracts, 86 proteins from mitotic phase egg extracts, and 93 proteins from both. Thirty-three proteins were pulled down by SUMO1, 85 proteins by SUMO2, and 228 proteins by both. We validated the sumoylation of five candidates, CKB, ATXN10, BTF3, HABP4, and BZW1, by co-transfecting them along with SUMO in HEK293T cells. Gene ontology analysis showed that SUMO substrates identified in this study were involved in diverse biological processes. Additionally, SUMO substrates identified from different cell cycle stages or pulled down by different SUMO homologs were enriched for distinct cellular components and functional categories. Our results comprehensively profile the sumoylation occurring in the Xenopus egg extract system.
Li Ma, Aaron Aslanian, Huaiyu Sun, Mingji Jin, Yu Shi, John R Yates, Tony Hunter

1795 related Products with: Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.

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#22781929   // To Up

Transcriptional response of the bovine endometrium and embryo to endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical inflammation of the uterine environment.

The aim of the present study was to analyse the effect of subclinical endometritis on endometrial and embryonic gene expression. A total of 49 cows at either Day 0 or Day 7 of the oestrous cycle (62-83 days post partum) following superovulation were classified as having subclinical endometritis (SE-0, SE-7) or a healthy endometrium (HE-0, HE-7) on the basis of endometrial cytological evaluation. Endometrial samples and associated embryos were subjected to global transcriptome analysis using the Bovine GeneChip (Affymetrix, Santa Clara, CA, USA) and aberrant transcript profiles were observed in SE-0 and SE-7 cows. At Day 0, 10 transcripts were found to be differentially expressed in endometrial samples. Specifically, the PDZK1, PXDN, DDHD2, GPLD1 and SULT1B1 genes were downregulated, whereas the PKIB, LOC534256, BT29392, LYZ and S100A14 genes were upregulated in SE-0 cows. Similarly, 11 transcripts were found to be differentially regulated on Day 7. Of these, GNPTG, BOLA-DQA5, CHD2, LOC541226, VCAM1 and ARHGEF2 were found to be downregulated, whereas PSTPIP2, BT236441 and MGC166084 were upregulated in SE-7 cows. Accordingly, endometrial health status affected the number of flushed, transferable embryos. In all, 20 genes were differentially regulated in blastocysts derived from HE-7 and SE-7 cows. Of these, GZMK, TCEAL4, MYL7, ADD3 and THEM50B were upregulated, whereas NUDCD2, MYO1E, BZW1, EHD4 and GZMB were downregulated. In conclusion, endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical endometritis is associated with changes in endometrial gene expression patterns, including genes involved in cell adhesion and immune modulation. Consequently, subclinical endometritis affects gene expression in embryos, including the expression of genes related to membrane stability, the cell cycle and apoptosis.
Michael Hoelker, Dessie Salilew-Wondim, Marc Drillich, Grosse-Brinkhaus Christine, Nasser Ghanem, Leopold Goetze, Dawit Tesfaye, Karl Schellander, Wolfgang Heuwieser

1483 related Products with: Transcriptional response of the bovine endometrium and embryo to endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical inflammation of the uterine environment.

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#21734401   2011/07/06 To Up

Novel autosomal recessive nonsyndromic hearing impairment locus DFNB90 maps to 7p22.1-p15.3.

A novel locus DFNB90 was mapped to 7p22.1-p15.3 by carrying out a genome scan in a multigenerational consanguineous family from Pakistan with autosomal recessive nonsyndromic hearing impairment (ARNSHI).DFNB90 is the eighth ARNSHI locus mapped to chromosome 7. A multipoint LOD score of 4.0 was obtained at a number of SNP marker loci spanning from rs1468996 (chromosome 7: 5.7 Mb) tors957960 (chromosome 7: 18.8 Mb). The 3-unit support interval and the region of homozygosity for DFNB90 spans from markers rs1553960 (chromosome 7: 4.9 Mb) to rs206198 (chromosome 7: 20.3 Mb). Candidate genes ACTB, BZW, OCM, MACC1, NXPH1, PRPS1L1, RAC1 and RPA3, which lie within the DFNB90 region, were sequenced and no potentially causal variants were identified.
Ghazanfar Ali, Kwanghyuk Lee, Paula B Andrade, Sulman Basit, Regie Lyn P Santos-Cortez, Leon Chen, Musharraf Jelani, Muhammad Ansar, Wasim Ahmad, Suzanne M Leal

2693 related Products with: Novel autosomal recessive nonsyndromic hearing impairment locus DFNB90 maps to 7p22.1-p15.3.

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#19446954   2009/05/15 To Up

BZW1, a novel proliferation regulator that promotes growth of salivary muocepodermoid carcinoma.

Mucoepidermoid carcinoma (MEC) is the most common primary malignancy in the salivary glands; however, its oncogenesis is poorly understood. Here, we show that the homo sapiens basic leucine zipper and W2 domains 1 (BZW1 or BZAP45) is a critical factor in the regulation of MEC growth. Firstly, BZW1 was highly expressed in MEC tissues and cells, determined by quantitative real time polymerase chain reaction. Higher levels of BZW1 proteins were detected in MEC tissues, particularly in high-grade MEC, by immunohistochemistry. Furthermore, down-regulation of BZW1 expression by infection with BZW1-specific RNAi-expressing lentivirus inhibited Mc3 cell proliferation and colony formation in vitro. In addition, down-regulation of BZW1 expression arrested Mc3 cell cycling at the G(0)/G(1) phase and mitigated Mc3 cell migration and invasiveness in vitro, determined by wound healing and transwell invasion assays. Importantly, down-regulation of BZW1 expression significantly reduced the tumorigenicity of Mc3 cells in vivo, evidenced by the slow progression of small tumors induced by BZW1-specific RNAi expressing Mc3 cells. Apparently, BZW1 is a novel factor, promoting the growth of MEC cells. Our findings may provide a base for design of new strategy for diagnosis and therapy for human MEC.
Shaoqing Li, Zhiguo Chai, Yan Li, Daqing Liu, Zhongcheng Bai, Yan Li, Yunming Li, Zhenqiang Situ

2466 related Products with: BZW1, a novel proliferation regulator that promotes growth of salivary muocepodermoid carcinoma.

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