Search results for: AP3 Antibody
#38463854 
2024/02/23
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Immunogenicity and protective efficacy of Ag85A and truncation of PstS1 fusion protein vaccines against tuberculosis.
TLingyuan Zeng, Xiuling Ma, Mengjin Qu, Minghui Tang, Huoming Li, Chengrui Lei, Jiahong Ji, Hao Li
2227 related Products with: Immunogenicity and protective efficacy of Ag85A and truncation of PstS1 fusion protein vaccines against tuberculosis.
100ul1000 TESTS/0.65ml2100 ug50050 200ul100ug100 25 mg1000 tests10 mg
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#38377312 2024/02/20
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Combinatorial Biosynthesis of 3--Carbamoylmaytansinol by Rational Engineering of the Tailoring Steps of Ansamitocins.
CQingqing Liu, Yu Wang, Xin Xia, Zhongyue Li, Yaoyao Li, Yuemao Shen, Haoxin Wang
2398 related Products with: Combinatorial Biosynthesis of 3--Carbamoylmaytansinol by Rational Engineering of the Tailoring Steps of Ansamitocins.
5 G0.1 mg100 IU500 Units 100 G121100.00 ul
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#35316825 2022/03/22
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Increased yield of AP-3 by inactivation of asm25 in Actinosynnema pretiosum ssp. auranticum ATCC 31565.
Asamitocins are maytansinoids produced by Actinosynnema pretiosum ssp. auranticum ATCC 31565 (A. pretiosum ATCC 31565), which have a structure similar to that of maytansine, therefore serving as a precursor of maytansine in the development of antibody-drug conjugates (ADCs). Currently, there are more than 20 known derivatives of ansamitocins, among which ansamitocin P-3 (AP-3) exhibits the highest antitumor activity. Despite its importance, the application of AP-3 is restricted by low yield, likely due to a substrate competition mechanism underlying the synthesis pathways of AP-3 and its byproducts. Given that N-demethylansamitocin P-3, the precursor of AP-3, is regulated by asm25 and asm10 to synthesize AGP-3 and AP-3, respectively, asm25 is predicted to be an inhibitory gene for AP-3 production. In this study, we inactivated asm25 in A. pretiosum ATCC 31565 by CRISPR-Cas9-guided gene editing. asm25 depletion resulted in a more than 2-fold increase in AP-3 yield. Surprisingly, the addition of isobutanol further improved AP-3 yield in the asm25 knockout strain by more than 6 times; in contrast, only a 1.53-fold increase was found in the WT strain under the parallel condition. Thus, we uncovered an unknown function of asm25 in AP-3 yield and identified asm25 as a promising target to enhance the large-scale industrial production of AP-3.Hong Cheng, Guoqing Xiong, Yi Li, Jiaqi Zhu, Xianghua Xiong, Qingyang Wang, Liancheng Zhang, Haolong Dong, Chen Zhu, Gang Liu, Huipeng Chen
1736 related Products with: Increased yield of AP-3 by inactivation of asm25 in Actinosynnema pretiosum ssp. auranticum ATCC 31565.
10mg 1 G 5 G 5 G 25 MG1 g25 g25 mg300 units25 g100ug
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#32179024 2020/03/13
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Metabolomics analysis of Actinosynnema pretiosum with improved AP-3 production by enhancing UDP-glucose biosynthesis.
Ansamitocin P-3 (AP-3) shows strong anticancer effects and has used as a payload for antibody-drug conjugates. Our previous study have shown that although genetically engineered Actinosynnema pretiosum strains with enhanced UDP-glucose (UDPG) biosynthesis displayed improved AP-3 production compared to the wild-type strain, the increase in yield was far from meeting the industrial demand. In this study, comparative metabolomics analysis complemented with quantitative real-time PCR analysis was performed for the wild-type strain and two mutants (OpgmOugp, ΔzwfΔgnd) to identify possible metabolic bottlenecks and non-intuitive targets for further enhancement of AP-3 production. We observed that enhancing intracellular UDPG availability facilitated the accumulation of intracellular N-demethyl-AP-3 and AP-3, where the transporting of them outside the cell still needs to be developed. We also found that the UDPG biosynthesis was closely associated with the availability of fructose in the medium and a suitable fructose feeding strategy could promote the further improvement of AP-3 titer. In addition, pathway abundance analysis revealed that undesired fatty acid accumulation and down-regulation of amino acid metabolism may be unfavorable for ansamitocin biosynthesis in later stage of production. These results indicate that genetic modification of the UDPG biosynthetic pathways may have pleiotropic effects on AP-3 production. Efforts must be made to eliminate these newly identified metabolic bottlenecks to boost AP-3 production in A. pretiosum.Ting Liu, Ziwen Jin, Ziwei Wang, Jun Chen, Liu-Jing Wei, Qiang Hua
2754 related Products with: Metabolomics analysis of Actinosynnema pretiosum with improved AP-3 production by enhancing UDP-glucose biosynthesis.
1 G100ug Lyophilized100ug Lyophilized250 mg2 g 5 G5 g1 module100ug Lyophilized 1 G25 g 25 MG
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#31587244 2019/10/06
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Current Anti-HPA-1a Standard Antibodies React with the β3 Integrin Subunit but not with αIIbβ3 and αvβ3 Complexes.
Fetal/neonatal alloimmune thrombocytopenia (FNAIT) results from maternal alloantibodies (abs) reacting with fetal platelets expressing paternal human platelet antigens (HPAs), mostly HPA-1a. Anti-HPA-1a abs, are the most frequent cause of severe thrombocytopenia and intracranial hemorrhage (ICH).Behnaz Bayat, Annalena Traum, Heike Berghöfer, Silke Werth, Jieging Zhu, Gregor Bein, Ulrich J Sachs, Sentot Santoso
1261 related Products with: Current Anti-HPA-1a Standard Antibodies React with the β3 Integrin Subunit but not with αIIbβ3 and αvβ3 Complexes.
1 mg100 μg0.1 mg100 ug Product tipe: Anti1 mL1 ml100.00 ug100.00 ug1.0ml100 1 mg
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#31380292 2019/07/16
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Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens , and .
and are the fungal pathogens responsible for most cases of invasive aspergillosis (IA). Early detection of the circulating antigen galactomannan (GM) in serum allows the prompt application of effective antifungal therapy, thus improving the survival rate of IA patients. However, the use of monoclonal antibodies (mAbs) for the diagnosis of IA is often associated with false positives due to cross-reaction with bacterial polysaccharides. More specific antibodies are therefore needed. Here we describe the characterization of the -specific mAb AP3 (IgG1κ), including the precise identification of its corresponding antigen. The antibody was generated using cell wall fragments and was shown to bind several species. Immunofluorescence microscopy revealed that AP3 binds a cell wall antigen, but immunoprecipitation and enzyme-linked immunosorbent assays showed that the antigen is also secreted into the culture medium. The inability of AP3 to bind the galactofuranose (Gal )-deficient mutant Δ confirmed that Gal residues are part of the epitope. Several lines of evidence strongly indicated that AP3 recognizes the Gal residues of -linked glycans on proteins. Glycoarray analysis revealed that AP3 recognizes oligo-[β-D-Gal-1,5] sequences containing four or more residues with longer chains more efficiently. We also showed that AP3 captures GM in serum, suggesting it may be useful as a diagnostic tool for patients with IA.Max Schubert, Sheng Xue, Frank Ebel, Annegret Vaggelas, Vadim B Krylov, Nikolay E Nifantiev, Ivana Chudobová, Stefan Schillberg, Greta Nölke
2339 related Products with: Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens , and .
100 ul100 ul100 ul100 ul100 ul100 ul100 ul100 ul100 ul100 ul100 ul
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#31371564 2019/08/01
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Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG.
To describe phenotypes, treatment response, and outcomes of autoimmunity targeting a synaptic vesicle coat protein, the neuronal (B2) form of adaptor protein-3 (AP3).Josephe A Honorat, A Sebastian Lopez-Chiriboga, Thomas J Kryzer, Lars Komorowski, Madeleine Scharf, Shannon R Hinson, Vanda A Lennon, Sean J Pittock, Christopher J Klein, Andrew McKeon
2881 related Products with: Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG.
100 ug100 ug100ug100ug Lyophilized100ug Lyophilized1 Set1 Set1 Set1000mg1 Set100
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#31022732 //
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Effect of αvβ3 Integrin Expression and Activity on Intraocular Pressure.
To determine the effects of αvβ3 integrin expression and activation on intraocular pressure (IOP).Jennifer A Faralli, Mark S Filla, Donna M Peters
1250 related Products with: Effect of αvβ3 Integrin Expression and Activity on Intraocular Pressure.
100ug Lyophilized100ug Lyophilized10 mg96 samples100 assays100ul 96 Tests 96 Wells/Kit 6 ml Ready-to-use 100ug Lyophilized
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#29561802 2018/03/21
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Selection and Characterization of Single-Stranded DNA Aptamers Binding Human B-Cell Surface Protein CD20 by Cell-SELEX.
The B-lymphocyte antigen (CD20) is a suitable target for single-stranded (ss) nucleic acid oligomer (aptamers). The aim of study was selection and characterization of a ssDNA aptamer against CD20 using Cell-Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX). The cDNA clone of CD20 (pcDNA-CD20) was transfected to human embryonic kidney (HEK293T) cells. Ten rounds of Cell-SELEX was performed on recombinant HEK-CD20 cells. The final eluted ssDNA pool was amplified and ligated in T/A vector for cloning. The plasmids of positive clones were extracted, sequenced and the secondary structures of the aptamers predicted using DNAMAN software. The sequencing results revealed 10 different types; three of them had the highest thermodynamic stability, named AP-1, AP-2 and AP-3. The AP-1 aptamer was the most thermodynamically stable one (ΔGAP-1 = -10.87 kcal/mol) with the highest binding affinity to CD20 (96.91 ± 4.5 nM). Since, the CD20 is a suitable target for recognition of B-Cell. The selected aptamers could be comparable to antibodies with many advantages. The AP-1, AP-2 and AP-3 could be candidate instead of antibodies for diagnostic and therapeutic applications in immune deficiency, autoimmune diseases, leukemia and lymphoma.Mansoureh Haghighi, Hossein Khanahmad, Abbasali Palizban