Only in Titles

Search results for: Anti-C-Reactive Protein produced in goat Antibody

paperclip

Error loading info... Pleas try again later.
paperclip

#35386964   2021/09/30 To Up

Allergen Risk Assessment for Specific Allergy to Small Ruminant's Milk: Development of Sensitive Immunoassays to Detect Goat's and Sheep's Milk Contaminations in Dairy Food Matrices.

Despite a high level of sequence identity between cow's, goat's, and sheep's milk (CM, GM, and SM, respectively) proteins, some patients tolerant to CM are allergic to GM and SM. In most cases, this specificity is due to the presence of IgE antibodies that bind only to caprine and ovine caseins. The patients may then develop severe allergic reactions after ingestion of CM products contaminated with low amounts of GM or SM. We thus aimed to develop an assay able to detect traces of caprine/ovine β-caseins in different food matrices, irrespective of the presence of the bovine homolog. We produced monoclonal antibodies (mAbs) specific to caprine caseins in mice tolerized to the bovine whole casein then sensitized to the caprine whole casein. In order to develop a two-site immunometric assay, we selected mAbs that could discriminate the caprine β-casein from its bovine homolog. Characteristics and performances of two tests were determined with various dairy products. Results were analyzed in relation with the IgE-immunoreactivity of the food matrices, thanks to sera from CM, GM/SM allergic patients. Our two-site immunometric assays demonstrated a high sensitivity with a detection limit of 1.6-3.2 ng/mL of caprine and ovine β-caseins. The tests were able to detect contaminations of GM in CM at the ppm level. Heat-treatment, ripening and coagulation processes, usually applied to dairy products that exhibit a very high IgE-immunoreactivity, did not impair the test sensitivity. These quantitative assays could then be useful for the risk assessment of food products potentially contaminated with GM and SM in order to prevent adverse reactions in patients specifically allergic to these milks.
Hervé Bernard, Stéphane Hazebrouck, Nicolas Gaiani, Karine Adel-Patient

1799 related Products with: Allergen Risk Assessment for Specific Allergy to Small Ruminant's Milk: Development of Sensitive Immunoassays to Detect Goat's and Sheep's Milk Contaminations in Dairy Food Matrices.

100 μg1 mg100 mg1 mg

Related Pathways

paperclip

#35247007   2022/03/20 To Up

Immunocastration with gene vaccine (KISS1) induces a cell-mediated immune response in ram testis: A transcriptome evaluation.

Immunocastration vaccines achieve their effects through neutralization of the endogenous hormone by the humoral antibody produced against the immunized genes, but there is little information regarding cell-mediated immune response on the gonadal function of the immunized model is available. In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine. The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization. Transcriptome analysis of testicular tissues was carried out to identify the expressions of protein-coding genes involved in cellular immunity. The results showed that we successfully constructed the KISS1 immune castration ram model, in which testicular growth and development, testosterone and kisspeptin-54 levels, and sexual function were suppressed in immunized rams (p < .05). Using Hiseq™ 2000 high sequencing for ram testicular, we identified 21 differentially expressed genes (DEGs) related to cellular immunity, of which, 14 genes were upregulated and seven genes were downregulated in the testis of the immunized group (p < .05). The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed that these differentially expressed genes were enriched in the antigen presentation process mediated by MHC class I and the cytotoxic pathway mediated by natural killer cells. It is concluded that KISS1 gene vaccine induced the cell-mediated immune response in testicular tissue to suppress reproductive activities in rams.
Sohail Ahmed, Dongdong Bo, Jiayu Zhao, Guiqiong Liu, Yi Ding, Xunping Jiang, Teketay Wassie, Haijing Jing

1830 related Products with: Immunocastration with gene vaccine (KISS1) induces a cell-mediated immune response in ram testis: A transcriptome evaluation.

1 kit96 samples2 Pieces/Box100ug Lyophilizedcase

Related Pathways

paperclip

#35185884   2022/02/03 To Up

Development and Characterization of New Monoclonal Antibodies Against Porcine Interleukin-17A and Interferon-Gamma.

Current research efforts require a broad range of immune reagents, but those available for pigs are limited. The goal of this study was to generate priority immune reagents for pigs and pipeline them for marketing. Our efforts were aimed at the expression of soluble swine cytokines and the production of panels of monoclonal antibodies (mAbs) to these proteins. Swine interleukin-17A (IL-17A) and Interferon-gamma (IFNγ) recombinant proteins were produced using yeast expression and used for monoclonal antibody (mAb) production resulting in panels of mAbs. We screened each mAb for cross-species reactivity with orthologs of IL-17A or IFNγ and checked each mAb for inhibition by other related mAbs, to assign mAb antigenic determinants. For porcine IL-17A, the characterization of a panel of 10 mAbs identified eight different antigenic determinants; interestingly, most of the mAbs cross-reacted with the dolphin recombinant ortholog. Likewise, the characterization of a panel of nine anti-PoIFNγ mAbs identified four different determinants; most of the mAbs cross-reacted with dolphin, bovine, and caprine recombinant orthologs. There was a unique reaction of one anti-PoIFNγ mAb that cross-reacted with the zebrafish recombinant ortholog. The αIL-17A mAbs were used to develop a quantitative sandwich ELISA detecting the yeast expressed protein as well as native IL-17A in stimulated peripheral blood mononuclear cell (PBMC) supernatants. Our analyses showed that phorbol myristate acetate/ionomycin stimulation of PBMC induced significant expression of IL-17A by CD3+ T cells as detected by several of our mAbs. These new mAbs expand opportunities for immunology research in swine.
Jean N Manirarora, Kristen E Walker, Veerupaxagouda Patil, Gourapura J Renukaradhya, Joanna LaBresh, Yvonne Sullivan, Ore Francis, Joan K Lunney

1256 related Products with: Development and Characterization of New Monoclonal Antibodies Against Porcine Interleukin-17A and Interferon-Gamma.

100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug1 ml100.00 ug100.00 ug100.00 ug100.00 ug

Related Pathways

paperclip

#34871629   2021/12/03 To Up

Development and evaluation of an indirect ELISA based on recombinant structural protein σC to detect antibodies against avian orthoreovirus.

The avian orthoreovirus (ARV) causes large economic losses to poultry industry around the whole world. ARV viral proteins can be classified into three sizes: λ (large), μ (medium) and σ (small). σC, one of the capsid protein of ARV, contains various specific neutralizing epitopes and can induce robust immune responses in infected chickens. An indirect enzyme-linked immunosorbent assay (I-ELISA) was established using the recombinant σC protein. The optimal dilutions of antigen, serum and conjugate goat anti-chicken IgY conjugate were 2.5 μg/ml, 1:80 and 1:4000, respectively. The optimal antigen coating condition was 4℃ for overnight. Hyperimmune serum of high-yielding hens of different age produced stronger reaction against σC. Chicken serum samples were randomly collected and total coincidence rate between commercial kit and σC-ARV-ELISA kit was 97.8%. Importantly, those discrepant serums were further verified using Western blot assay and three of them were determined to be positive. Thus, the method based on σC protein has higher sensitivity compared with the commercial kit and provide a rapid and simple method for serodiagnosis of ARV, contributing to convenient epidemiological monitoring of ARV.
Zhiyuan He, Sirui Yang, Jin Xiao

1166 related Products with: Development and evaluation of an indirect ELISA based on recombinant structural protein σC to detect antibodies against avian orthoreovirus.

100 200ug200 ug1000 TESTS/0.65ml0.1mg96 tests1 mg0.1 mg100ug Lyophilized100 100 200 ug

Related Pathways

paperclip

#34631844   2021/09/22 To Up

Proteome Modulation in Peripheral Blood Mononuclear Cells of Ruminants Vaccinated Goats and Sheep.

In the present study, healthy goats and sheep ( = 5) that were confirmed negative for ruminants virus (PPRV) antibodies by monoclonal antibody-based competitive ELISA and by serum neutralization test and for PPRV antigen by s-ELISA were vaccinated with Sungri/96. A quantitative study was carried out to compare the proteome of peripheral blood mononuclear cells (PBMCs) of vaccinated goat and sheep [5 days post-vaccination (dpv) and 14 dpv] vs. unvaccinated (0 day) to divulge the alteration in protein expression following vaccination. A total of 232 and 915 proteins were differentially expressed at 5 and 14 dpv, respectively, in goats. Similarly, 167 and 207 proteins were differentially expressed at 5 and 14 dpv, respectively, in sheep. Network generated by Ingenuity Pathway Analysis was "infectious diseases, antimicrobial response, and inflammatory response," which includes the highest number of focus molecules. The bio functions, cell-mediated immune response, and humoral immune response were highly enriched in goats at 5 dpv and at 14 dpv. At the molecular level, the immune response produced by the PPRV vaccine virus in goats is effectively coordinated and stronger than that in sheep, though the vaccine provides protection from virulent virus challenge in both. The altered expression of certain PBMC proteins especially ISG15 and IRF7 induces marked changes in cellular signaling pathways to coordinate host immune responses.
Sajad Ahmad Wani, Amit Ranjan Sahu, Raja Ishaq Nabi Khan, Manas Ranjan Praharaj, Shikha Saxena, Kaushal Kishor Rajak, Dhanavelu Muthuchelvan, Aditya Sahoo, Bina Mishra, R K Singh, Bishnu Prasad Mishra, Ravi Kumar Gandham

2943 related Products with: Proteome Modulation in Peripheral Blood Mononuclear Cells of Ruminants Vaccinated Goats and Sheep.

30ml15ml100ml30ml15ml100ml100ml10ml96 tests1mg10ml

Related Pathways

paperclip

#34454575   2021/08/28 To Up

Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China.

Tibet Orbivirus (TIBOV) is a recently discovered Orbivirus known to infect cattle, Asian buffalo and goats in south-western China. It was first isolated from mosquitoes and subsequently from biting midges (Culicoides spp.) in Yunnan, China, indicating that it is an arbovirus. Little is known of its potential to cause disease, but the economic importance of related viruses promoted an investigation of potential Culicoides spp. vectors of TIBOV.
Ying Liang Duan, Zhen Xing Yang, Glenn Bellis, Le Li

2989 related Products with: Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China.

1 Set1 Set 5 G1 Set1 g1 Set1mg 100ul50 96 wells (1 kit)

Related Pathways

paperclip

#34298067   2021/07/21 To Up

Development of rELISA using novel markers for the diagnosis of paratuberculosis.

Paratuberculosis is one of the complex livestock infections whose control has largely been hampered due to the lack of efficacious diagnostics. Present study optimized plate ELISA assay for the diagnosis and screening of paratuberculosis using recombinant secretory proteins. Five secretory antigens (2677c, 3547c, 4308c, 1693c, and 2168c) were produced in the recombinant system using the E. coli host and used for the optimization of the assay. These proteins were selected because of their prior proven specificity and antigenicity as humoral immunity markers. The assay was first optimized using traditional ELISA reader and then the performance was evaluated using a handheld ELISA reader. Findings were identical in both traditional ELISA reader as well as handheld ELISA reader. Optimized ELISA was found reproducible using different batches of the recombinant antigens as well as in terms of the inter and intra assay %CV values. The present ELISA has a sensitivity and specificity of 91.6% and 100%, respectively. Also, rELISA revealed AUC and Youden index J of 0.95 and 0.91, respectively. In conclusion, assay conditions of MAP-recombinant protein-based ELISA were optimized and the optimized ELISA ODs can be read using portable handheld ELISA reader. Thereby, opening a future window to develop assay for onsite testing.
Mukta Jain, Amit Kumar, Rathnagiri Polavarapu, Saurabh Gupta, G K Aseri, Deepansh Sharma, Jagdip Singh Sohal

1050 related Products with: Development of rELISA using novel markers for the diagnosis of paratuberculosis.

5 G0.1 ml500 Units5 mg300 units100ug100μg96 Well10 mg 500 ml

Related Pathways

paperclip

#34224759   2021/07/02 To Up

Expression and characterization of a novel single-chain anti-vascular endothelial growth factor antibody in the goat milk.

Vascular endothelial growth factor (VEGF) has essential functions in angiogenesis, endothelial cell proliferation, migration, and tumor invasion. Different approaches have been developed to suppress tumor angiogenesis, which is considered a hallmark of cancer. Anti-VEGF monoclonal antibodies constitute an important strategy for cancer immunotherapy, which has been produced on several platforms. In this study, a novel single-chain anti-VEGF monoclonal antibody (scVEGFmAb) was produced in the goat mammary gland by adenoviral transduction. scVEGFmAb was purified by affinity chromatography. N-glycans were analyzed by exoglycosidase digestion and hydrophilic interaction ultra-performance liquid chromatography coupled to electrospray ionization mass spectrometry. The biological activity of scVEGFmAb was assessed by scratch and mouse aortic ring assays. scVEGFmAb was produced at 0.61 g/L in the goat milk, and its purification rendered 95 % purity. N-glycans attached to scVEGFmAb backbone were mainly neutral biantennary core fucosylated with Galβ1,4GlcNAc motif, and charged structures were capped with Neu5Ac and Neu5Gc. The chimeric molecule significantly prevented cell migration and suppressed microvessel sprouting. These results demonstrated for the first time the feasibility of producing an anti-VEGF therapeutic antibody in the milk of non-transgenic goats with the potential to counteract tumor angiogenesis.
María J Leiva, Natalie C Parra, María A Contreras, Kaio C S Tavares, Luis Macaya, Leonardo Martins, Saúl Gaudencio, Ángela Hidalgo, Radoslaw P Kozak, Alaín González, Oliberto Sánchez, Jorge R Toledo, Raquel Montesino

1119 related Products with: Expression and characterization of a novel single-chain anti-vascular endothelial growth factor antibody in the goat milk.

100 μg100.00 ug0.1 mg0.1 mg100 μg100.00 ug0.1 mg0.1 mg100.00 ug0.1 mg

Related Pathways

paperclip

#34103465   2021/06/09 To Up

Challenges in the Management of an Echis coloratus Adult Snakebite Victim at a Tertiary Care Hospital: A Case Report.

BACKGROUND The World Health Organization has set clear guidelines for the management of snakebite envenomation. However, challenges have been reported in the clinical application of guidelines, such as identification of the biting snake, hypersensitivity reactions to the antivenom, and influence of repeated antivenom administration during hospital stay. This report aims to discuss how these challenges can affect patient management and to highlight improvement opportunities. CASE REPORT An 18-year-old man presented to the Emergency Department without remarkable signs of envenomation following a snakebite. An initial dose of antivenom was given despite the misidentification of snake species. An allergic reaction developed and was successfully managed. Following admission, the coagulation profile and local tissue reaction worsened. Upon consulting the Drug and Poison Information Center, it was discovered that a subtherapeutic dose of antivenom was administered. The patient was rechallenged after the administration of premedication. Coagulation profile could not be maintained; therefore, 2 extra doses of antivenom were administered, resulting in sustained improvement in local tissue reaction and coagulation profile. CONCLUSIONS First, victims presenting without signs and symptoms of envenomation may benefit from close monitoring for late presentation of envenomation signs. Second, dosing guidelines are suggested to consider Institute of Safe Medication Practices recommendations for order sets to reduce the possibility of medication errors. Third, premedication may be an effective alternative in patients who develop allergic reaction to the locally produced equine antivenom in the setting of absent goat-derived antivenom. Lastly, antivenom administration should be titrated to patient response even if it occurs over several days.
Ahmed Abdeldayem, Alhanouf Ayed Alanazi, Jawaher N Aljabri, Ijaz Abid

2248 related Products with: Challenges in the Management of an Echis coloratus Adult Snakebite Victim at a Tertiary Care Hospital: A Case Report.



Related Pathways