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Search results for: Anti adenovirus type 5 hexon Monoclonal

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#24935650   2014/06/16 To Up

A recombinant adenovirus-based vector elicits a specific humoral immune response against the V3 loop of HIV-1 gp120 in mice through the "Antigen Capsid-Incorporation" strategy.

Due to potential advantages, human adenoviral vectors have been evaluated pre-clinically as recombinant vaccine vectors against several cancers and infectious diseases, including human immunodeficiency virus (HIV) infection. The V3 loop of HIV-1 glycoprotein 120 (gp120) contains important neutralizing epitopes and plays key roles in HIV entry and infectivity.
Linlin Gu, Valentina Krendelchtchikova, Alexandre Krendelchtchikov, Robert A Oster, Kohtaro Fujihashi, Qiana L Matthews

1902 related Products with: A recombinant adenovirus-based vector elicits a specific humoral immune response against the V3 loop of HIV-1 gp120 in mice through the "Antigen Capsid-Incorporation" strategy.

100 5mg100ug/vial100 µg100 µg100 µg1100ug/vial1 mg

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#19961802   // To Up

[Preparation, characterization and application of anti-human adenovirus monoclonal antibody with neutralization activity.].

To prepare, identify and apply anti-human adenoviru(HAdv)neutralization monoclonal antibody(mAb).
Si-Xiu Li, Guo-Cheng Zhang, Dong-Liang Xu, Xiao-Jing Nie, Xiao-Qing Li, Zhi-Hua Wang, Xue-Hong Zhang

2809 related Products with: [Preparation, characterization and application of anti-human adenovirus monoclonal antibody with neutralization activity.].

25 µg100 TESTS25 µg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#19065543   // To Up

Designed recombinant adenovirus type 5 vector induced envelope-specific CD8(+) cytotoxic T lymphocytes and cross-reactive neutralizing antibodies against human immunodeficiency virus type 1.

A monoclonal antibody (mAb) 2F5 binds to the membrane-proximal external region (MPER) of the transmembrane subunit gp41 of human immunodeficiency virus type 1 (HIV-1) is known to broadly neutralize HIV-1 strains. The Adenovirus type 5 vector (Ad5) has been widely applied for HIV-1 vaccine, and hexon hypervariable region 5 (HVR5) is exposed on viral surface and easily target host immune responses against Ad5.
Takehiro Ura, Atsushi Yoshida, Ke-Qin Xin, Shinji Yoshizaki, Sayaka Yashima, Shinya Abe, Hiroyuki Mizuguchi, Kenji Okuda

1570 related Products with: Designed recombinant adenovirus type 5 vector induced envelope-specific CD8(+) cytotoxic T lymphocytes and cross-reactive neutralizing antibodies against human immunodeficiency virus type 1.

1mg100ug100ug1 mL1mg500 500 1 mL50100 TESTS0.1 ml50

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#9139083   // To Up

Rapid detection and serotyping of adenovirus by direct immunofluorescence.

Four fluorescent antibody reagents were evaluated for their suitability for the identification of adenovirus isolates by immunofluorescence. The antibodies used in the reagents consist of monoclonal antibodies against adenovirus type 3 (Ad3), Ad4, Ad8, and adenoviruses of subgroup C (Ad1,2,5,6), serotypes known to occur in outbreaks of disease. Most of the monoclonal antibodies employed were reactive against type-specific antigens found on the hexon protein. Reagents employing two noncompeting anti-hexon antibodies were more sensitive than reagents prepared with only one monoclonal antibody, although both types of reagents exhibited a high degree of specificity. Five hundred and seventeen adenovirus isolates (359 of which had previously been typed by other methods) and 46 nonadenovirus isolates were examined with all four type-specific reagents in parallel with an adenovirus group-specific reagent. The results indicate that direct typing of adenovirus isolates is feasible, leading to significant savings in time compared to other typing methods and should contribute to the management of certain adenovirus infections, particularly during outbreaks.
S R Wood, I R Sharp, E O Caul, I Paul, A S Bailey, M Hawkins, S Pugh, J Treharne, S Stevenson

1454 related Products with: Rapid detection and serotyping of adenovirus by direct immunofluorescence.

1 kit1 mg5mg100μg 96 Tests 1 mL1000 tests2x96 well plates100ug100/kit100ul200 ug

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#1696636   // To Up

Antibodies with specificities against a dispase-produced 15-kilodalton hexon fragment neutralize adenovirus type 2 infectivity.

During the entrance of adenovirus type 2 into cells, it has been suggested that the virion undergoes a conformational change. In this investigation, we have further characterized the hypothetical conformational change, which the structural protein hexon undergoes in response to low pH. From pH 5.0 to pH 6.0, the proteolytic enzyme dispase cleaved the hexon into a few distinct fragments with a dominating low-molecular-weight fragment with a molecular weight of 15,000 (15K peptide), whereas between pH 6.5 and pH 8.0, the cleavage of the hexon was negligible. The degradation of the hexon with dispase at low pH was not due to an increased activity or alteration of the active site of dispase at low pH. The 15K fragment was identified as a segment of the N-terminal part of the hexon polypeptide beginning at amino acid residue 5. An immune serum produced in response to acid-treated and glutaraldehyde-fixed hexons contained a small amount of antibodies directed towards the 15K fragment, as judged by Western immunoblotting. An anti-15K antibody fraction was isolated by affinity chromatography by removing antibodies recognizing the hexon in the alkaline configuration. Such antibodies displayed a higher relative titer at pH 5.0 than at pH 7.5 in an enzyme-linked immunosorbent assay. The isolated antibodies showed a specific neutralizing capacity five times higher than that of the corresponding unfractionated polyclonal anti-hexon serum; however, the neutralizing ability was independent of pH. The neutralization of adenovirus type 2 infection by the isolated anti-15K antibodies implies that the N-terminal end of the hexon may play a critical role in the early steps of the virion-cell interaction.
M J Varga, T Bergman, E Everitt

2170 related Products with: Antibodies with specificities against a dispase-produced 15-kilodalton hexon fragment neutralize adenovirus type 2 infectivity.

0.2 mg100ul0.2 mg100ul0.2 mg100ul0.2 mg100ul100 μg500 50 100ug/vial

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#2621062   // To Up

Anti-idiotype response to selected neutralizing monoclonal antibodies generated against adenovirus type 2 fiber.

A series of monoclonal antibodies was prepared against purified adenovirus type 2 fiber. These antibodies were produced by both classical techniques and by electrofusion. Fifty antibodies were obtained, and five were selected which had the following characteristics: (i) they were able to reduce adenovirus 2 infection of Hep-2 cells; (ii) they had different light-chain structures and (iii) they did not react with denatured fiber. The five antibodies (AdF18, AdF38, AdF41, AdF2D3, AdF3B1) recognized between them three periodate-insensitive epitopes on the fiber. AdF18 bound to both adenovirus type 2 and type 5; all the others were specific to type 2. Polyclonal anti-idiotype antibodies to these antibodies were produced in rabbits and were purified extensively on immunoaffinity columns. Anti-idiotypes to AdF38 and AdF41 were able to inhibit virus infectivity, and anti-AdF41 completed with fiber for binding to the surface of Hep-2 cells.
B G Talbot, J Hallée

1443 related Products with: Anti-idiotype response to selected neutralizing monoclonal antibodies generated against adenovirus type 2 fiber.

100ul100.00 ug200ug100ul1 mg100ul100ul1 mg100.00 ug100.00 ug100ul100.00 ug

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