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Search results for: Albumin, Rat Antibody

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#34533288   2021/09/17 To Up

Development and characterization of a unique anti-IgE mouse monoclonal antibody cross-reactive between human and canine IgE.

The efficacy assessment of human anti-IgE monoclonal antibodies (mAbs) in animal models before clinical trials is hampered due to the lack of cross-reactivity of anti-IgE mAbs between species.
Akiko Kumagai, Takuya Nara, Mizuho Uematsu, Yoko Kakinuma, Takashi Saito, Kenichi Masuda

2254 related Products with: Development and characterization of a unique anti-IgE mouse monoclonal antibody cross-reactive between human and canine IgE.

0.1 mg25 Mg0.1 mg100μl0.1 mg100μl0.1 mg0.25 mg1 mg0.1 mg100μl0.1 mg

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#34497854   2021/08/27 To Up

Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats.

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( = 6); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.
Mohammed A Alsieni, Haddad A El Rabey, Abdulbasit I Al-Sieni, Madeha N Al-Seeni

2833 related Products with: Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats.

5 G100ul50 ug 25 mg100.00 ul1000 TESTS/0.65ml 5 G100ug15mg

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#34381367   2021/07/26 To Up

Modified Huangqi Chifeng Decoction Attenuates Proteinuria by Reducing Podocyte Injury in a Rat Model of Immunoglobulin a Nephropathy.

Modified Huangqi Chifeng decoction (MHCD) has been used to reduce proteinuria in immunoglobulin A nephropathy (IgAN) for many years. Previously, we have demonstrated its protective role in glomerular mesangial cells. Podocyte injury, another key factor associated with proteinuria in IgAN, has also attracted increasing attention. However, whether MHCD can reduce proteinuria by protecting podocytes remains unclear. The present study aimed to investigate the protective effects of MHCD against podocyte injury in a rat model of IgAN. To establish the IgAN model, rats were administered bovine serum albumin, carbon tetrachloride, and lipopolysaccharide. MHCD in three doses or telmisartan was administered once daily for 8 weeks ( = 10 rats/group). Rats with IgAN developed proteinuria at week 6, which worsened over time until drug intervention. After drug intervention, MHCD reduced proteinuria and had no effect on liver and kidney function. Furthermore, MHCD alleviated renal pathological lesions, hyperplasia of mesangial cells, mesangial matrix expansion, and podocyte foot process fusion. Western blot analysis revealed that MHCD increased the expression of the podocyte-associated proteins nephrin and podocalyxin. Additionally, we stained podocyte nuclei with an antibody for Wilms' tumor protein one and found that MHCD increased the podocyte number in rats with IgAN. In conclusion, these results demonstrate that MHCD attenuates proteinuria by reducing podocyte injury.
Meiying Chang, Bin Yang, Liusheng Li, Yuan Si, Mingming Zhao, Wei Hao, Jinning Zhao, Yu Zhang

1159 related Products with: Modified Huangqi Chifeng Decoction Attenuates Proteinuria by Reducing Podocyte Injury in a Rat Model of Immunoglobulin a Nephropathy.

100 UG18 kgs100.00 ug4 Membranes/Box100 μg100ug Lyophilized100 μg100ug100 μg1 Set

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#34245083   2021/07/09 To Up

Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model.

Experimental studies demonstrated antibodies against matrix and coating of polyester-based vascular prostheses. Thus, this study examined associations of these antibodies with serum cytokines (IL-2, IL-4, and IL-10) and local inflammatory reactions. Rats (n = 8/group) intramuscularly received prosthesis segments [PET-C, PET-G, and PET-A groups: polyethylene terephthalate (PET)-based prostheses coated with bovine collagen and gelatin or human serum albumin, respectively; uncoated polytetrafluoroethylene-based (PTFE) prosthesis], with sham-operated controls. Blood was drawn pre-operatively and weekly until day 22. Polymer-specific or coating-specific antibodies and cytokines were detected by enzyme immunoassays, inflammatory reactions were immunohistochemically evaluated on day 23. Polymer-specific antibodies were detected in all PET-groups using uncoated PET as antigenic target, but not for PTFE or controls, coating-specific antibodies only for PET-A. IL-10 was increased in all PET-groups and correlated with polymer-specific antibodies for PET-G and PET-A. IL-2 was increased for PET-A, but overall correlated with PET-specific antibodies. IL-4 remained unchanged in all groups. Intense local inflammatory reactions (ED1 /ED2 macrophages and T lymphocytes) were found within all PET-groups, but only minor for PTFE or controls. In conclusion, PET-specific antibodies were associated with increased IL-10 and along with concurrent coating-specific antibodies also with increased IL-2, indicating a specific T cell response. Thus, matrix and/or coating of polymeric vascular prostheses elicit distinct systemic immune reactions, probably influencing local inflammatory reactions.
Ronny Köhler, Christopher Pohl, Uwe Walschus, Roland Zippel, Lutz Wilhelm, Andreas Hoene, Maciej Patrzyk, Michael Schlosser

1187 related Products with: Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model.

100 UG4 Arrays/Slide4 Membranes/Box16 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box4 Arrays/Slide4 Membranes/Box4 Membranes/Box16 Arrays/Slide4 Membranes/Box

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#34237365   2021/07/05 To Up

Protein microbeadification to achieve highly concentrated protein formulation with reversible properties and in vivo pharmacokinetics after reconstitution.

A protein precipitation technique was optimized to produce biophysically stable 'protein microbeads', applicable to highly concentrated protein formulation. Initially, production of BSA microbeads was performed using rapid dehydration by vortexing in organic solvents followed by cold ethanol treatment and a vacuum drying. Out of four solvents, n-octanol produced the most reversible microbeads upon reconstitution. A Shirasu porous glass (SPG) membrane emulsification technique was utilized to enhance the size distribution and manufacturing process of the protein microbeads with a marketized human IgG solution. Process variants such as dehydration time, temperature, excipients, drying conditions, and initial protein concentration were evaluated in terms of the quality of IgG microbeads and their reversibility. The hydrophobized SPG membrane produced a narrow size distribution of the microbeads, which were further enhanced by shorter dehydration time, low temperature, minimized the residual solvents, lower initial protein concentration, and addition of trehalose to the IgG solution. Final reversibility of the IgG microbeads with trehalose was over 99% at both low and high protein concentrations. Moreover, the formulation was highly stable under repeated mechanical shocks and at an elevated temperature compared to its liquid state. Its in vivo pharmacokinetic profiles in rats were consistent before and after the 'microbeadification'.
Nam Ah Kim, Hyun Woo Yu, Ga Yeon Noh, Sang-Koo Park, Wonku Kang, Seong Hoon Jeong

1313 related Products with: Protein microbeadification to achieve highly concentrated protein formulation with reversible properties and in vivo pharmacokinetics after reconstitution.

50100 2 Pieces/Box10100 1 mg2 Pieces/Box1 mg1mg1 mg100ug Lyophilized

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#34202668   2021/06/24 To Up

Early Renoprotective Effect of Ruxolitinib in a Rat Model of Diabetic Nephropathy.

Diabetic kidney disease (DKD) is still one of the unresolved major complications of diabetes mellitus, which leads ultimately to end-stage renal disease in both type 1 and type 2 diabetes patients. Available drugs that suppress the renin-angiotensin system have partially minimized the disease impact. Yet, there is an unmet need for new therapeutic interventions to protect the kidneys of diabetic patients. In DN, glomerular sclerosis and tubulointerstitial fibrosis are mediated through several pathways, of which JAK/STAT is a key one. The current study explored the potential renoprotective effect of the JAK1/JAK2 inhibitor ruxolitinib (at doses of 0.44, 2.2, and 4.4 mg·kg) compared to that of enalapril at a dose of 10 mg·kg, in a rat model of streptozotocin-induced diabetes mellitus over 8 weeks. The effect of ruxolitinib was assessed by determining urinary albumin/creatinine ratio, serum level of cystatin, and levels of TGF-β1, NF-κB, and TNF-α in renal tissue homogenates by biochemical assays, the glomerular sclerosis and tubulointerstitial fibrosis scores by histological analysis, and fibronectin, TGF-β1, and Vimentin levels by immunohistochemical staining with the respective antibodies. Our results revealed a significant early favorable effect of a two-week ruxolitinib treatment on the renal function, supported by a decline in the proinflammatory biomarkers of DKD. This pre-clinical study suggests that the renoprotective effect of ruxolitinib in the long term should be investigated in animals, as this drug may prove to be a potential option for the treatment of diabetic kidney disease.
Mohamed M El-Kady, Reham A Naggar, Maha Guimei, Iman M Talaat, Olfat G Shaker, Maha Saber-Ayad

2808 related Products with: Early Renoprotective Effect of Ruxolitinib in a Rat Model of Diabetic Nephropathy.

100 UG18 kgs100ug Lyophilized200 assays100ug Lyophilized100 μg100ug Lyophilized100 μg100ug100ug Lyophilized

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#34112923   2021/06/10 To Up

In situ strategy for biomedical target localization via nanogold nucleation and secondary growth.

Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H/K-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.
Akira Sawaguchi, Takeshi Kamimura, Nobuyasu Takahashi, Atsushi Yamashita, Yujiro Asada, Hiroyuki Imazato, Fumiyo Aoyama, Akiko Wakui, Takeshi Sato, Narantsog Choijookhuu, Yoshitaka Hishikawa

2632 related Products with: In situ strategy for biomedical target localization via nanogold nucleation and secondary growth.

0.1ml (1mg/ml)100 ml100.00 ug0.1ml20 ug 5 G0.1 ml250 mg100.00 ugProtein50ul (1mg/ml)0.1ml

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#33910203   2021/04/28 To Up

Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis.

Necrotizing crescentic glomerulonephritis (GN) associated with anti-neutrophil cytoplasmic antibodies (ANCA) against myeloperoxidase (MPO) is a devastating disease that quickly progresses to kidney failure. Current therapies are broadly immunosuppressive and associated with adverse effects. We wanted to set up a model that could be suitable for testing narrowly targeted therapies.
Domenico Cerullo, Daniela Rottoli, Daniela Corna, Paola Rizzo, Mauro Abbate, Daniela Macconi, Ariela Benigni, Giuseppe Remuzzi, Carlamaria Zoja

2476 related Products with: Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis.

100μg100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100μg100ug100μg100ug100ug Lyophilized100μg100ug

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#33864519   2021/04/17 To Up

Immunohistochemical localization and pharmacokinetics of the anti-MRSA drug teicoplanin in rat kidney using a newly developed specific antibody.

We prepared a polyclonal antibody against a teicoplanin (TEIC)-bovine serum albumin conjugate that was specific to both conjugated and free forms of TEIC. We demonstrated that this antibody could be used to detect the time-dependent localization of TEIC in rat kidneys. Immunohistochemistry revealed immunoreactivity specifically in the microvilli and apical cytoplasm of epithelial cells in proximal tubule segments S1 and S2, 1 h after intravenous TEIC injection, with higher staining intensity in the S2 segments. The epithelial cells of S3 segments showed moderate immunostaining with a few cells exhibiting nuclear staining. Furthermore, we found that the distal tubules and collecting ducts contained both TEIC-positive and -negative cells. TEIC immunoreactivity decreased rapidly over time; only weak staining remained in the S3 segments, distal tubules, and collecting ducts 24 h after administration. No staining was detected 7 days after injection. These results were significantly different from those of our previous study obtained using vancomycin, which showed moderate staining in the proximal tubule segments S1 and S2, distal tubules, and the collecting ducts 8 days after administration. The lower TEIC accumulation in tissues may account for a lower risk of adverse events compared to that using vancomycin.
Yutaro Yamamoto, Yuta Yamamoto, Tetsuya Saita, Masashi Shin

2315 related Products with: Immunohistochemical localization and pharmacokinetics of the anti-MRSA drug teicoplanin in rat kidney using a newly developed specific antibody.

100 UG100ug100ug100ug100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug100ug

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#33851410   2021/04/14 To Up

High-resolution three-dimensional visualization of hepatic sinusoids in cirrhotic rats via serial histological sections.

As a specialized intraparenchymal vascular conduit, hepatic sinusoids play a key role in liver microcirculation. This study aimed to explore the three-dimensional (3D) morphological changes of cirrhotic sinusoids by serial histological sections.
Jing-Yi Liu, Wen-Juan Lv, Jian-Bo Jian, Xiao-Hong Xin, Xin-Yan Zhao, Chun-Hong Hu

1151 related Products with: High-resolution three-dimensional visualization of hepatic sinusoids in cirrhotic rats via serial histological sections.



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