Search results for: APOA4
#33962068 2021/05/04 To Up
Proteomic analysis of liver tissues in chicken embryo at Day 16 and Day 20 reveals antioxidant mechanisms.To investigate the mechanisms of the defense system and antioxidant defense system during chicken embryo development, protein profiling of liver tissues in chicken embryo at Day 16 and Day 20 was conducted. TMT was used to analyze the liver tissues proteomes with significantly different activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in chicken embryo. PRM was operated to validate the target differentially abundant proteins (DAPs) using the same samples. The result showed a total of 34 DAPs were identified. Among these, 9 were upregulated and 25 were downregulated. The screened DAPs strictly related to regulation of oxidoreductase activity (DDO and GAS2L1), response to stress (ERAD2 and SAA), immune system process (GAL3 and PDCD4), and lipid regulation and metabolism (ETNPPL, APOV1, LIPM, and APOA4). These analyses indicated that the antioxidant enzyme activity of chicken embryo is regulated through different pathways. Correlation analysis revealed a linear relationship between mRNA and protein expression and 12 genes (ORM1, C8B, KPNA2, CA4, C1S, SULT1B, ETNPPL, ERCC6L, DDO, SERPINF1, VAT1L, and APOA4) were detected to be differently expressed both at mRNA and protein levels. In consequence, these findings are an important resource that can be used in future studies of antioxidant mechanisms in chicken embryo. BIOLOGICAL SIGNIFICANCE: The genetic mechanisms of antioxidant activity are still unclear in chicken embryo. In the article, the combined transcriptomic and proteomic analysis is used to further explore potential signaling pathways and differentially abundant proteins related to antioxidant activity. These findings will facilitate a better understanding of the mechanism and these DAPs can be further investigated as candidate markers to predict the activity of antioxidant enzymes.
Shaohua Yang, Zhangqi Wei, Jianxin Wu, Miaomiao Sun, Yilong Ma, Guoqing Liu
2150 related Products with: Proteomic analysis of liver tissues in chicken embryo at Day 16 and Day 20 reveals antioxidant mechanisms.20ml20mg20mg5mg900 tests5mg50mg10mg
#33949105 2021/05/05 To Up
Protein Kinase D2 drives chylomicron-mediated lipid transport in the intestine and promotes obesity.Lipids are the most energy-dense components of the diet, and their overconsumption promotes obesity and diabetes. Dietary fat content has been linked to the lipid processing activity by the intestine and its overall capacity to absorb triglycerides (TG). However, the signaling cascades driving intestinal lipid absorption in response to elevated dietary fat are largely unknown. Here, we describe an unexpected role of the protein kinase D2 (PKD2) in lipid homeostasis. We demonstrate that PKD2 activity promotes chylomicron-mediated TG transfer in enterocytes. PKD2 increases chylomicron size to enhance the TG secretion on the basolateral side of the mouse and human enterocytes, which is associated with decreased abundance of APOA4. PKD2 activation in intestine also correlates positively with circulating TG in obese human patients. Importantly, deletion, inactivation, or inhibition of PKD2 ameliorates high-fat diet-induced obesity and diabetes and improves gut microbiota profile in mice. Taken together, our findings suggest that PKD2 represents a key signaling node promoting dietary fat absorption and may serve as an attractive target for the treatment of obesity.
Jonathan Trujillo-Viera, Rabih El-Merahbi, Vanessa Schmidt, Till Karwen, Angel Loza-Valdes, Akim Strohmeyer, Saskia Reuter, Minhee Noh, Magdalena Wit, Izabela Hawro, Sabine Mocek, Christina Fey, Alexander E Mayer, Mona C Löffler, Ilka Wilhelmi, Marco Metzger, Eri Ishikawa, Sho Yamasaki, Monika Rau, Andreas Geier, Mohammed Hankir, Florian Seyfried, Martin Klingenspor, Grzegorz Sumara
2095 related Products with: Protein Kinase D2 drives chylomicron-mediated lipid transport in the intestine and promotes obesity.72 tests0.2 mg1 Set100 ul1 Set1 Set1 Set100ug Lyophilized1 Set1 Set1 Set
#33925510 2021/04/27 To Up
Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112).Hepatocyte growth factor (HGF) is an endogenously induced bioactive molecule that has strong anti-apoptotic and tissue repair activities. In this research, we identified APOA4 as a novel pharmacodynamic (PD) marker of the recombinant human HGF (rh-HGF), E3112.
Sotaro Motoi, Mai Uesugi, Takashi Obara, Katsuhiro Moriya, Yoshihisa Arita, Hideaki Ogasawara, Motohiro Soejima, Toshio Imai, Tetsu Kawano
2730 related Products with: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112).5 x 50 ug2ug x 2050 ug1020 ug2ug x 2020 ug1010ug250IU5 x 50 ug5ug
#33894376 2021/04/22 To Up
Proteomic and lipidomic analyses reveal saturated fatty acids, phosphatidylinositol, phosphatidylserine, and associated proteins contributing to intramuscular fat deposition.Intramuscular fat (IMF) content is an important factor in porcine meat quality. Previous studies have screened multiple candidate genes related to IMF deposition, but the lipids that affect IMF deposition and their lipid-protein network remain unknown. In this study, we performed proteomic and lipidomic analyses of the longissimus dorsi (LD) muscle from high-IMF (IMFH) and low-IMF (IMF-L) groups of Xidu black pigs. Eighty-eight proteins and 143 lipids were differentially abundant between the groups. The differentially abundant proteins were found to be involved in cholesterol metabolism, the PPAR signaling pathway, and ferroptosis. The triacylglycerols (TAGs) upregulated in the IMF-H group were mainly shown to be synthesized by saturated fatty acids (SFAs), while the downregulated TAGs were mainly synthesized by polyunsaturated fatty acids (PUFAs). All differentially abundant phosphatidylinositols (PIs) and phosphatidylserines (PSs) were found to be upregulated in the IMF-H group. A correlation analysis of the proteomic and lipidomic revealed candidate proteins (APOA4, VDAC3, PRNP, CTSB, GSPT1) related to TAG, PI, and PS lipids. These results revealed differences in proteins and lipids between the IMF-H and IMF-L groups, which represent new candidate proteins and lipids that should be investigated to determine the molecular mechanisms controlling IMF deposition in pigs. SIGNIFICANCE: Intramuscular fat (IMF) is a key factor affecting meat quality, and meat with a higher IMF content can have a better flavor. In this study, proteomic results show that the ferroptosis pathway, including the PRNP, VDAC3 and CP proteins, affects IMF deposition. Lipid composition is the key factor affecting IMF deposition, but there are few reports on this. In this study, through lipidomic analysis, we suggest that saturated fatty acid (SFA), phosphatidylinositol (PI), and phosphatidylserine (PS) may contribute to IMF deposition. A correlation analysis reveals the potential regulatory network between lipids and proteins. This study clarifies the difference in protein and lipid compositions in longissimus dorsi (LD) muscle with high and low IMF contents. This information suggests that it would be beneficial to increase the intramuscular fat content of pork not only from a genetic perspective but also from a nutritional perspective.
Jiawei Zhou, Yu Zhang, Junjing Wu, Mu Qiao, Zhong Xu, Xianwen Peng, Shuqi Mei
1131 related Products with: Proteomic and lipidomic analyses reveal saturated fatty acids, phosphatidylinositol, phosphatidylserine, and associated proteins contributing to intramuscular fat deposition.100ul1,000 tests2.5 mg 10 UG100ul100g10 mg0.1 mg 5 G10 1,000 tests
#33893167 2021/04/23 To Up
S1P-Gα signaling inhibits astrocytic glutamate uptake and mitochondrial oxygen consumption.Glutamate is the principal excitatory neurotransmitter in the human brain. Following neurotransmission, astrocytes remove excess extracellular glutamate to prevent neurotoxicity. Glutamate neurotoxicity has been reported in multiple neurological diseases including multiple sclerosis (), representing a shared neurodegenerative mechanism. A potential modulator of glutamate neurotoxicity is the bioactive lysophospholipid sphingosine 1-phosphate () that signals through five cognate G protein-coupled receptors (), S1P - S1P, however, a clear link between glutamate homeostasis and S1P signaling has not been established. Here, S1P receptor knock-out mice, primary astrocyte cultures, and receptor-selective chemical tools were used to examine the effects of S1P on glutamate uptake. S1P inhibited astrocytic glutamate uptake in a dose-dependent manner and increased mitochondrial oxygen consumption, primarily through S1P Primary cultures of wild-type mouse astrocytes expressed S1P transcripts, and selective deletion of S1P and/or S1P in cerebral cortical astrocytes, did not alter S1P-mediated, dose-dependent inhibition of glutamate uptake. Pharmacological antagonists, S1P-null astrocytes, and Gα hemizygous-null astrocytes indicated that S1P-Gα-Rho/ROCK signaling was primarily responsible for the S1P-dependent inhibition of glutamate uptake. In addition, S1P exposure increased mitochondrial oxygen consumption rates () in wild-type astrocytes, and reduced OCRs in S1P-null astrocytes, implicating receptor selective metabolic consequences of S1P-mediated glutamate uptake inhibition. Astrocytic S1P-S1P signaling increased extracellular glutamate, which could contribute to neurotoxicity. This effect was not observed with the FDA-approved S1P receptor modulators, siponimod and fingolimod. Development and use of S1P-selective antagonists may provide a new approach to reduce glutamate neurotoxicity in neurological diseases.Extracellular glutamate is excitotoxic and its levels are controlled by astrocyte uptake. Sphingosine 1-phosphate (S1P) is a bioactive lipid originating from cell membrane sphingolipids that associates with carrier molecules like albumin, ApoM, and ApoA4 to produce cellular effects. S1P signals extracellularly through five GPCRs and it is found in higher concentrations in neurological diseases like multiple sclerosis where excitotoxic neurodegeneration has been implicated. Here we show that astrocytic S1P activation by S1P results in glutamate uptake inhibition to promote excitotoxic damage. S1P receptor modulators, including approved drugs for treating multiple sclerosis ( fingolimod (FTY720) and siponimod (BAF312)), do not engage S1P, thus avoiding glutamate uptake inhibition. S1P antagonists may provide a means to reduce S1P-induced glutamate neurotoxicity and ameliorate neurological diseases.
Deepa Jonnalagadda, Yasuyuki Kihara, Richard Rivera, Jerold Chun
2405 related Products with: S1P-Gα signaling inhibits astrocytic glutamate uptake and mitochondrial oxygen consumption.10 mg100ul100 tests 50 UG10 mg 100ul100 mg100 ug2 Pieces/Box2 Pieces/Box1,000 tests
#33714802 2021/03/05 To Up
Shotgun proteomics for the preliminary identification of biomarkers of beef sensory tenderness, juiciness and chewiness from plasma and muscle of young Limousin-sired bulls.Label free shotgun proteomics was used to analyse plasma and Longissimus muscle biopsies of Limousin-sired bulls, classified as 5 high-quality and 5 low-quality meat based on sensory texture traits (tenderness, juiciness and chewiness). A total of 31 putative protein biomarkers (16 in plasma and 15 in muscle) differed significantly in abundance between the two quality groups. The proteins were associated with muscle structure, energy metabolism, heat shock proteins, oxidative stress and proteolysis related pathways. Among them, B2M, AHSG, APOA4 and HP-20 (plasma), PFKM, MYH2, PTER, GSTM1 and MYPN (muscle) were good predictors of the three texture quality traits. Further, significant correlations were identified for FETUB, SERPINA7, ASL, TREH, HP, HP-25, AZGP1, APCS and SYT15, which are novel biomarkers from plasma that warrant further evaluation. This study is a significant step forward in elucidating proteomic profiles in bovine bio-fluids and muscle tissue, which may ultimately provide opportunities to processors for early assessment of beef sensory quality.
Yao Zhu, Mohammed Gagaoua, Anne Maria Mullen, Didier Viala, Dilip K Rai, Alan L Kelly, David Sheehan, Ruth M Hamill
2542 related Products with: Shotgun proteomics for the preliminary identification of biomarkers of beef sensory tenderness, juiciness and chewiness from plasma and muscle of young Limousin-sired bulls.96T 5 G100ug100ug100 mg1000 tests500 MG25 mg10 mg50 ug 50 mg100ul
#33640722 2021/02/18 To Up
Decreased serum apolipoprotein A4 as a potential peripheral biomarker for patients with schizophrenia.Recent evidence supports an association between lipid metabolism dysfunction and the pathology of schizophrenia which has led to the search for peripheral blood-based biomarkers. The purpose of this study was to investigate the proteins involved in lipid metabolism (especially apolipoprotein) and to explore their potential as biomarkers for schizophrenia. Using multiple reaction monitoring mass spectrometry (MRM-MS), we quantified 22 proteins in serum samples of 109 healthy controls (HCs) and 111 patients with schizophrenia (SCZ), who were divided into discovery and validation sets. We found serum apolipoprotein A4 (ApoA4) to be significantly decreased in SCZ patients compared to HCs (p=1.61E-05). Moreover, the serum ApoA4 level served as an effective diagnostic tool, achieving area under the receiver operating characteristic curves (AUROC) of 0.840 in the discovery set and 0.791 in the validation set. Additionally, apolipoprotein F (ApoF), angiotensinogen (AGT), and alpha1-antichymotrypsin (ACT) levels were significantly higher in patients with schizophrenia than in healthy controls. These proteins combined with ApoA4, provided higher diagnostic accuracy for schizophrenia in the discovery set (AUROC=0.901) and in the validation set (AUROC=0.879). Our results suggest that the serum level of ApoA4 is a novel potential biomarker for schizophrenia. The proteins identified in this study expand the pool of biomarker candidates for schizophrenia and may be linked to the underlying mechanism of the disease.
Minghui Li, Xuhan Yang, Liya Sun, Ying Qing, Xiaowen Hu, Jie Jiang, Dandan Wang, Gaoping Cui, Yan Gao, En Zhang, Juan Zhang, Yong Yang, Chunling Wan
1715 related Products with: Decreased serum apolipoprotein A4 as a potential peripheral biomarker for patients with schizophrenia.100 assays96 well1 kit100 tests1 kit1 kit(96 Wells)1,000 tests100 plates1 kit10 plates1 kit1,000 tests
#33602229 2021/02/18 To Up
Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.To validate markers for cervical carcinoma (CC) and precancerous lesions related with HPV infections.
Yi Hao, Ming Ye, Xiaona Chen, Hongli Zhao, Ayshamgul Hasim, Xia Guo
1435 related Products with: Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.100tests100ug2.5 mg25 assays10 mg1 kit100ul 5 G
#33585012 // To Up
Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers.We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver.
Akram Safaei, Afsaneh Arefi Oskouie, Seyed Reza Mohebbi, Zahra Razaghi, Naser Nejadi
1757 related Products with: Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers.1 g430 tests1 mg100.00 ug1 module1 mg1 moduleOne 96-Well Strip Micropl5 1 G1 kit(96 Wells)
#33561811 2021/01/05 To Up
Gene networks and pathways for plasma lipid traits via multitissue multiomics systems analysis.Genome-wide association studies (GWASs) have implicated ∼380 genetic loci for plasma lipid regulation. However, these loci only explain 17-27% of the trait variance, and a comprehensive understanding of the molecular mechanisms has not been achieved. In this study, we utilized an integrative genomics approach leveraging diverse genomic data from human populations to investigate whether genetic variants associated with various plasma lipid traits, namely, total cholesterol, high and low density lipoprotein cholesterol (HDL and LDL), and triglycerides, from GWASs were concentrated on specific parts of tissue-specific gene regulatory networks. In addition to the expected lipid metabolism pathways, gene subnetworks involved in "interferon signaling," "autoimmune/immune activation," "visual transduction," and "protein catabolism" were significantly associated with all lipid traits. In addition, we detected trait-specific subnetworks, including cadherin-associated subnetworks for LDL; glutathione metabolism for HDL; valine, leucine, and isoleucine biosynthesis for total cholesterol; and insulin signaling and complement pathways for triglyceride. Finally, by using gene-gene relations revealed by tissue-specific gene regulatory networks, we detected both known (e.g., APOH, APOA4, and ABCA1) and novel (e.g., F2 in adipose tissue) key regulator genes in these lipid-associated subnetworks. Knockdown of the F2 gene (coagulation factor II, thrombin) in 3T3-L1 and C3H10T1/2 adipocytes altered gene expression of Abcb11, Apoa5, Apof, Fabp1, Lipc, and Cd36; reduced intracellular adipocyte lipid content; and increased extracellular lipid content, supporting a link between adipose thrombin and lipid regulation. Our results shed light on the complex mechanisms underlying lipid metabolism and highlight potential novel targets for lipid regulation and lipid-associated diseases.
Montgomery Blencowe, In Sook Ahn, Zara Saleem, Helen Luk, Ingrid Cely, Ville-Petteri Mäkinen, Yuqi Zhao, Xia Yang
1196 related Products with: Gene networks and pathways for plasma lipid traits via multitissue multiomics systems analysis.96T 50 UG 72 tests96T100ul96 wells (1 kit)1000pcs
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