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Search results for: antibodies

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#35045376   2022/01/13 To Up

Identification of anti-lipoarabinomannan antibodies against mannan core and their effects on phagocytosis of mycobacteria by human neutrophils.

Mycobacterium tuberculosis (MTB) and M. avium-intracellulare complex (MAC) enter host phagocytes, such as neutrophils through lipoarabinomannan (LAM) binding to pattern-recognition receptors, inducing innate immune responses including phagocytosis. Phagocytosis of mycobacteria by human neutrophils depends on the binding of α(1 → 2)-monomannose branching α(1 → 6)-mannan core of LAM/lipomannan (LM), a common component among mycobacterial species, to lactosylceramide (LacCer)-enriched lipid microdomains. We investigated the binding specificities of several anti-LAM antibodies (Abs) to LAMs/LM and found anti-LAM monoclonal IgMs TMDU3 and LA066 were directed against mannan core. Each IgM showed different binding specificity to mannan core. Confocal and stimulated emission depletion microscopy revealed TMDU3 and LA066 strongly bind to MTB and MAC, respectively. Flow cytometric analysis revealed human neutrophils do not express Dectin-2, DC-SIGN or mannose receptor. Furthermore, neutrophil phagocytosis of mycobacteria was markedly inhibited by TMDU3 and LA066, respectively. Similarly, treatment of each mAb with neutrophils reduced the numbers of intracellular MAC. Together, our results suggest that the interaction of LacCer-enriched lipid microdomains with mannan core and its blocking are therapeutic or diagnostic targets for both TB and non-tuberculous mycobacteria infection.
Hitoshi Nakayama, Eriko Oshima, Tomomi Hotta, Kei Hanafusa, Kota Nakamura, Noriko Yokoyama, Hideoki Ogawa, Kenji Takamori, Kazuhisa Iwabuchi

1356 related Products with: Identification of anti-lipoarabinomannan antibodies against mannan core and their effects on phagocytosis of mycobacteria by human neutrophils.

5000.1 mg1 ml200 TESTS50 100.00 ug1mg100 μg100 μg

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#35045269   2022/01/19 To Up

The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.

SARS-CoV-2 spike (S) glycoprotein mediates viral entry and membrane fusion. Its cleavage at S1/S2 and S2' sites during the biosynthesis in virus-producer cells and viral entry are critical for viral infection and transmission. In contrast, the biological significance of the junction region between both cleavage sites on S protein synthesis and function is less understood. By analyzing the conservation and structure of S protein, we find that intra-chain contacts formed by the conserved tyrosine (Y) residue 756 (Y756) with three α helices contribute to the spike's conformational stability. When Y756 is mutated to an amino acid residue that can provide hydrogen bonds, S protein could be expressed as a cleaved form, but not . Also, the L753 mutation linked to the Y756 hydrogen bond prevents the S protein from being cleaved. Y756 and L753 mutations alter S protein subcellular localization. Importantly, Y756 and L753 mutations are demonstrated to reduce the infectivity of the SARS-CoV-2 pseudoviruses by interfering with the incorporation of S protein into pseudovirus particles and causing the pseudovirus to lose its sensitivity to neutralizing antibodies. Furthermore, both mutations affect the assembly and production of SARS-CoV-2 virus-like particles in cell culture. Altogether, our findings reveal for the first time a critical role for the conserved L753-LQ-Y756 motif between S1/S2 and S2' cleavage sites in S proteins synthesis and processing as well as virus assembly and infection. The continuous emergence of SARS-CoV-2 virus variants such as delta or lambda lineage caused the continuation of the epidemic and challenged the effectiveness of these existing vaccines. Logically, the spike (S) protein mutation has attracted much concern. However, the key amino acids in S protein for its structure and function are still not very clear. Here, we discovered for the first time that the conserved residues Y756 and L753 at the junction between S1/S2 and S2' sites are very important as the S2' cleavage site R815 for the synthesis and processing of S protein such as protease cleavage, and then the mutations severely interfered with the incorporation of S protein into pseudotyped virus particles and SARS-CoV-2 virus-like particles. Consequently, we delineate the novel potential target for the design of broad-spectrum antiviral drugs in the future, especially in the emergence of SARS-CoV-2 variants.
Shouwen Du, Wang Xu, Yuhang Wang, Letian Li, Pengfei Hao, Mingyao Tian, Maopeng Wang, Tiyuan Li, Shipin Wu, Quan Liu, Jieying Bai, Xiaoyun Qu, Ningyi Jin, Boping Zhou, Ming Liao, Chang Li

1330 related Products with: The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.

20 ug Product tipe: Antib100 100 100 100 100 1000100500100 1000100

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#35045226   2022/01/19 To Up

Immunogenicity and Reactogenicity of Vaccine Boosters after Ad26.COV2.S Priming.

The Ad26.COV2.S vaccine, which was approved as a single-shot immunization regimen, has been shown to be effective against severe coronavirus disease 2019. However, this vaccine induces lower severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S)-specific antibody levels than those induced by messenger RNA (mRNA)-based vaccines. The immunogenicity and reactogenicity of a homologous or heterologous booster in persons who have received an Ad26.COV2.S priming dose are unclear.
Roos S G Sablerolles, Wim J R Rietdijk, Abraham Goorhuis, Douwe F Postma, Leo G Visser, Daryl Geers, Katharina S Schmitz, Hannah M Garcia Garrido, Marion P G Koopmans, Virgil A S H Dalm, Neeltje A Kootstra, Anke L W Huckriede, Melvin Lafeber, Debbie van Baarle, Corine H GeurtsvanKessel, Rory D de Vries, P Hugo M van der Kuy,

2098 related Products with: Immunogenicity and Reactogenicity of Vaccine Boosters after Ad26.COV2.S Priming.

50 ug 100ug50 ug 100ug50 ug 100ug96T100ug50

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#35045183   // To Up

Clinical Profile and Short-Term Outcome of SARS-CoV-2-Infected Neonates from a Government Medical College in West Bengal, India.

Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has led to a terrifying global pandemic. The presentations in neonates are varied with less case severity compared to adults.
Mukut Banerjee, Jonaki Pal, Tanushree Mondal, Taraknath Ghosh, Kaustav Nayek

1181 related Products with: Clinical Profile and Short-Term Outcome of SARS-CoV-2-Infected Neonates from a Government Medical College in West Bengal, India.

100 μg100 μg 0.1 mg

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#35044938   2021/12/31 To Up

COVID-19 in Malaysia: exposure assessment and prevention practices among healthcare workers at a teaching hospital.

During the second wave of the coronavirus disease 19 (COVID-19) pandemic, Malaysia reported several COVID-19 clusters related to healthcare workers. Thus, addressing and understanding the risk of exposure in healthcare workers is important to prevent future infection and reduce secondary COVID-19 transmission within the healthcare settings. In this study, we aim to assess exposure and prevention practices against COVID-19 among healthcare workers at the Hospital Canselor Tuanku Muhriz, a university teaching hospital based in Kuala Lumpur, Malaysia.
Nor Azila Muhammad Azami, Nor Azian Abdul Murad, Azmawati Mohammed Nawi, Sharifah Azura Salleh, Petrick Periyasamy, Najma Kori, Mohd Rohaizat Hasan, Norfazilah Ahmad, Anita Sulong, Hanita Othman, Tuti Ningseh Mohd Don, Nurul Syakima Ab Mutalib, Ezanee Azlina Mohamad Hanif, Siti Aishah Sulaiman, Nurul Syeefa' Zulkiflee, Abdul Rashid Abdul Kader, Abdul Halim Abdul Gafor, Hanafiah Haruna Rashid, Rahman Jamal

2281 related Products with: COVID-19 in Malaysia: exposure assessment and prevention practices among healthcare workers at a teaching hospital.

20 100 μg1 Set1 Set100 μg20 100 µl (2 mM)100 μg5 mg100 μg4 Membranes/Box5 mg

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#35044824   2022/01/19 To Up

Up-regulation of proBDNF/p75 signaling in antibody-secreting cells drives systemic lupus erythematosus.

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Wei-Yun Shen, Cong Luo, Plinio Reinaldo Hurtado, Xiao-Jing Liu, Ru-Yi Luo, Hui Li, Zhao-Lan Hu, Jun-Mei Xu, Elizabeth J Coulson, Ming Zhao, Xin-Fu Zhou, Ru-Ping Dai

1549 related Products with: Up-regulation of proBDNF/p75 signaling in antibody-secreting cells drives systemic lupus erythematosus.

96 wells1 Set1 Set1 Set1 Set50ug11 inhibitors100ug Lyophilized100ug100ug Lyophilized1 Set1 Set

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#35044813   2022/01/19 To Up

From structure to sequence: Antibody discovery using cryoEM.

One of the rate-limiting steps in analyzing immune responses to vaccines or infections is the isolation and characterization of monoclonal antibodies. Here, we present a hybrid structural and bioinformatic approach to directly assign the heavy and light chains, identify complementarity-determining regions, and discover sequences from cryoEM density maps of serum-derived polyclonal antibodies bound to an antigen. When combined with next-generation sequencing of immune repertoires, we were able to specifically identify clonal family members, synthesize the monoclonal antibodies, and confirm that they interact with the antigen in a manner equivalent to the corresponding polyclonal antibodies. This structure-based approach for identification of monoclonal antibodies from polyclonal sera opens new avenues for analysis of immune responses and iterative vaccine design.
Aleksandar Antanasijevic, Charles A Bowman, Robert N Kirchdoerfer, Christopher A Cottrell, Gabriel Ozorowski, Amit A Upadhyay, Kimberly M Cirelli, Diane G Carnathan, Chiamaka A Enemuo, Leigh M Sewall, Bartek Nogal, Fangzhu Zhao, Bettina Groschel, William R Schief, Devin Sok, Guido Silvestri, Shane Crotty, Steven E Bosinger, Andrew B Ward

1390 related Products with: From structure to sequence: Antibody discovery using cryoEM.

3 modules1 module2 modules1 module1 module1 module1 module1 module200 ug1 module100 ul1 module

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#35044724   2022/01/19 To Up

Antiphospholipid antibodies increase endometrial stromal cell decidualization, senescence and inflammation via TLR4, ROS and p38 MAP kinase signaling.

Miscarriage affects one in seven pregnancies and antiphospholipid autoantibodies (aPL) are one of the biggest risk factors for recurrent pregnancy loss. While aPL target the endometrial stroma, little is known about their impact. Endometrial stromal cells (EnSCs) undergo decidualization each menstrual cycle, priming the uterus to receive implanting embryos. Thus, appropriate decidualization and EnSC function is key for establishment of a successful pregnancy.
Mancy Tong, Teimur Kayani, Deidre M Jones, Jane E Salmon, Shannon Whirledge, Lawrence W Chamley, Vikki M Abrahams

2397 related Products with: Antiphospholipid antibodies increase endometrial stromal cell decidualization, senescence and inflammation via TLR4, ROS and p38 MAP kinase signaling.

2 Pieces/Box0.1 mg1 ml1000 TESTS/0.65ml2 Pieces/BoxOne Vial: 5 X 10^6 Cells 25 MG100 μg100ug100.00 ug25 mg

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#35044720   2022/01/19 To Up

High throughput glycosylation analysis of intact monoclonal antibodies by mass spectrometry coupled with capillary electrophoresis and liquid chromatography.

The analysis of monoclonal antibodies glycosylation is a crucial quality control attribute of biopharmaceutical drugs. High throughput screening approaches for antibody glycoform analysis are required in various stages of process optimization. Here, we present high throughput screening suitable mass spectrometry-based workflows for the analysis of intact antibody glycosylation out of cell supernatants. Capillary electrophoresis and liquid chromatography were coupled with quadrupole time-of-flight MS or Orbitrap MS. Both separation methods offer fast separation (10-15 min) and the capability to prevent the separated cell supernatant matrix to enter the MS by post-separation valving. Both MS instruments provide comparable results and both are sufficient to determine the glycosylation pattern of the five major glycoforms of the measured antibodies. However, the Orbitrap yields higher sensitivity of 25 μg/mL (CE-nanoCEasy-Orbitrap MS) and 5 μg/mL (LC-Orbitrap MS). Data processing was optimized for a faster processing and easier detection of low abundant glycoforms based on averaged charge-deconvoluted mass spectra. This approach combines a non-target glycoform analysis, while yielding the same glycosylation pattern as the traditional approach based on extracted ion traces. The presented methods enable the high throughput screening of the glycosylation pattern of antibodies down to low μg/mL-range out of cell supernatant without any sample preparation. This article is protected by copyright. All rights reserved.
Lukas Naumann, Patrick Schlossbauer, Florian Klingler, Friedemann Hesse, Kerstin Otte, Christian Neusüß

2481 related Products with: High throughput glycosylation analysis of intact monoclonal antibodies by mass spectrometry coupled with capillary electrophoresis and liquid chromatography.

100.00 ug100 ug2 Pieces/Box100.00 ug100 ug100.00 ug1 mg100 ug100.00 ug1 mg100.00 ug1 mg

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