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#32252065   2020/04/06 To Up

Vocal cord palsy in refractory anti-melanoma differentiation-associated protein 5 antibody-positive dermatomyositis.


Omar Suhail Alsaed, Laith Ishaq Alamlih, Khalifa Abdulsalam Farfar, Tasleem Raza N Mohd, Samar A Al Razaq Alemadi, Hani Ali Malallah

1762 related Products with: Vocal cord palsy in refractory anti-melanoma differentiation-associated protein 5 antibody-positive dermatomyositis.

100ug Lyophilized100ug Lyophilized100 μg100100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#32251978   2020/03/10 To Up

Recent advances in LC-MS based characterization of protein-based bio-therapeutics - mastering analytical challenges posed by the increasing format complexity.

Alongside the success of protein-based bio-therapeutics over the last decades and facilitated by advances both in protein engineering and manufacturing, new product formats progressively enter into the biopharmaceutical industry's pipelines with major implications on the analytical methods used for their characterization. While conventional approaches have proved sufficient for standard (IgG-like) molecules, the increased complexity of novel formats requires proper adjustments of the employed methodologies, in particular with regard to separation-based techniques coupled to UV/FLD detection. After introducing the status quo for the characterization of biopharmaceuticals in quality control settings, this review provides a comprehensive portrayal of emerging LC-MS based technologies, which have already demonstrated their potential to complement the existing analytical toolbox. In this context, the benefits of native LC-MS and two-/multidimensional LC-MS applications to assess product attributes while preserving the higher-order structure are discussed based on challenges arising from the analysis of complex product formats.
Tobias Graf, Katrin Heinrich, Ingrid Grunert, Harald Wegele, Markus Haberger, Patrick Bulau, Michael Leiss

1349 related Products with: Recent advances in LC-MS based characterization of protein-based bio-therapeutics - mastering analytical challenges posed by the increasing format complexity.

1 Set1 Set1 Set1 Set1 Set1 Set1 Set100.00 ug1 Set1 Set1 Set

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#32251928   2020/04/01 To Up

Characterisation and evaluation of the regenerative capacity of Stro-4+ enriched bone marrow mesenchymal stromal cells using bovine extracellular matrix hydrogel and a novel biocompatible melt electro-written medical-grade polycaprolactone scaffold.

Many skeletal tissue regenerative strategies centre around the multifunctional properties of bone marrow derived stromal cells (BMSC) or mesenchymal stem/stromal cells (MSC)/bone marrow derived skeletal stem cells (SSC). Specific identification of these particular stem cells has been inconclusive. However, enriching these heterogeneous bone marrow cell populations with characterised skeletal progenitor markers has been a contributing factor in successful skeletal bone regeneration and repair strategies. In the current studies we have isolated, characterised and enriched ovine bone marrow mesenchymal stromal cells (oBMSCs) using a specific antibody, Stro-4, examined their multipotential differentiation capacity and, in translational studies combined Stro-4+ oBMSCs with a bovine extracellular matrix (bECM) hydrogel and a biocompatible melt electro-written medical-grade polycaprolactone scaffold, and tested their bone regenerative capacity in a small in vivo, highly vascularised, chick chorioallantoic membrane (CAM) model and a preclinical, critical-sized ovine segmental tibial defect model. Proliferation rates and CFU-F formation were similar between unselected and Stro-4+ oBMSCs. Col1A1, Col2A1, mSOX-9, PPARG gene expression were upregulated in respective osteogenic, chondrogenic and adipogenic culture conditions compared to basal conditions with no significant difference between Stro-4+ and unselected oBMSCs. In contrast, proteoglycan expression, alkaline phosphatase activity and adipogenesis were significantly upregulated in the Stro-4+ cells. Furthermore, with extended cultures, the oBMSCs had a predisposition to maintain a strong chondrogenic phenotype. In the CAM model Stro-4+ oBMSCs/bECM hydrogel was able to induce bone formation at a femur fracture site compared to bECM hydrogel and control blank defect alone. Translational studies in a critical-sized ovine tibial defect showed autograft samples contained significantly more bone, (4250.63 mm, SD = 1485.57) than blank (1045.29 mm, SD = 219.68) ECM-hydrogel (1152.58 mm, SD = 191.95) and Stro-4+/ECM-hydrogel (1127.95 mm, SD = 166.44) groups. Stro-4+ oBMSCs demonstrated a potential to aid bone repair in vitro and in a small in vivo bone defect model using select scaffolds. However, critically, translation to a large related preclinical model demonstrated the complexities of bringing small scale reported stem-cell material therapies to a clinically relevant model and thus facilitate progression to the clinic.
C Black, J M Kanczler, M C de Andrés, L J White, F M Savi, O Bas, S Saifzadeh, J Henkel, A Zannettino, S Gronthos, M A Woodruff, D W Hutmacher, R O C Oreffo

1934 related Products with: Characterisation and evaluation of the regenerative capacity of Stro-4+ enriched bone marrow mesenchymal stromal cells using bovine extracellular matrix hydrogel and a novel biocompatible melt electro-written medical-grade polycaprolactone scaffold.

96T100ul200ug200ul1000 TESTS/0.65ml100ul25 mg1000 tests10 mg10 mg

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#32251869   2020/03/14 To Up

Brain magnetic resonance imaging features in multiple sclerosis and neuromyelitis optica spectrum disorders patients with or without aquaporin-4 antibody in a Latin American population.

There is scarce evidence comparing the behavior in magnetic resonance (MRI) between positive and negative aquaporin-4 antibody neuromyelitis optica spectrum disorders (P-NMOSD and NNMOSD, respectively). The aim of this study was to describe and compare MRI features through a quantitative and qualitative analysis between P-NMOSD and NNMOSD patients in a cohort from Latin American (LATAM) patients.
Facundo Silveira, Agustín Pappolla, Francisco Sánchez, Vanessa Daccach Marques, Ibis Soto de Castillo, Verónica Tkachuk, Alejandro Caride, Maria C Castillo, Edgardo Cristiano, Camila de Aquino Cruz, Gabriel Braga Diégues Serva, Antonio Carlos Dos Santos, Carolina Lavigne Moreira, Pablo A López, Liliana Patrucco, Omaira Molina, Juan Pablo Pettinicchi, Edgar Carnero Contentti, Juan Ignacio Rojas

1891 related Products with: Brain magnetic resonance imaging features in multiple sclerosis and neuromyelitis optica spectrum disorders patients with or without aquaporin-4 antibody in a Latin American population.

16 Arrays/Slide

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#32251821   2020/04/03 To Up

Blocking VEGF by Bevacizumab Attenuates VEGF-Induced Vasospasm After Experimental Subarachnoid Hemorrhage in Rabbits.

Vasospasm after subarachnoid hemorrhage (SAH) plays a vital role in the development of delayed cerebral ischemia. Anti-VEGF antibodies, like bevacizumab, may attenuate VEGF- stimulated angiogenesis, reduced vascular cell proliferation, and improve vasospasm after SAH.
Umut Dogu Akturk, Cengiz Tuncer, Huseyin Bozkurt, Omer Selcuk Sahin, Husamettin Bulut, Ata Arikok, Cem Dinc, Bora Gurer, Erhan Turkoglu

2311 related Products with: Blocking VEGF by Bevacizumab Attenuates VEGF-Induced Vasospasm After Experimental Subarachnoid Hemorrhage in Rabbits.

100.00 ug50.00 ug200.00 ug2.00 ug250ul1.00 ml10 100ug Lyophilized

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#32251798   2020/04/03 To Up

Diagnostic value and dynamic variance of serum antibody in coronavirus disease 2019.

To investigate the diagnostic value of serological test and dynamitic variance of serum antibody in coronavirus disease 2019 (COVID-19).
Yujiao Jin, Miaochan Wang, Zhongbao Zuo, Chaoming Fan, Fei Ye, Zhaobin Cai, Ying Wang, Huaizhong Cui, Kenu Pan, Aifang Xu

2426 related Products with: Diagnostic value and dynamic variance of serum antibody in coronavirus disease 2019.

500 tests500 tests 50 UG96T1 Set100 ml

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#32251791   2020/04/03 To Up

A first Case of Meningitis/Encephalitis associated with SARS-Coronavirus-2.

Novel coronavirus (SARS-Coronavirus-2:SARS-CoV-2) which emerged in Wuhan, China, has spread to multiple countries rapidly. We report the first case of meningitis associated with SARS-CoV-2 who was brought in by ambulance due to a convulsion accompanied by unconsciousness. He had never been to any foreign countries. He felt generalized fatigue and fever (day 1). He saw doctors nearby twice (day2 and 5) and was prescribed Laninamivir and antipyretic agents, His family visited his home and found that he was unconsciousness and lying on the floor in his vomit. He was immediately transported to this hospital by ambulance (day 9). Under emergency transport, he had transient generalized seizures that lasted about a minute. He had obvious neck stiffness. The specific SARS-CoV-2 RNA was not detected in the nasopharyngeal swab but was detected in a CSF. Anti- HSV 1 and varicella-zoster IgM antibodies were not detected in serum samples. A brain MRI showed hyperintensity along the wall of right lateral ventricle and hyperintense signal changes in the right mesial temporal lobe and hippocampus, suggesting the possibility of SARS-CoV-2 meningitis. This case warns the physicians of patients who have CNS symptoms.
Takeshi Moriguchi, Norikazu Harii, Junko Goto, Daiki Harada, Hisanori Sugawara, Junichi Takamino, Masateru Ueno, Hiroki Sakata, Kengo Kondo, Natsuhiko Myose, Atsuhito Nakao, Masayuki Takeda, Hirotaka Haro, Osamu Inoue, Katsue Suzuki-Inoue, Kayo Kubokawa, Shinji Ogihara, Tomoyuki Sasaki, Hiroyuki Kinouchi, Hiroyuki Kojin, Masami Ito, Hiroshi Onishi, Tatsuya Shimizu, Yu Sasaki, Nobuyuki Enomoto, Hiroshi Ishihara, Shiomi Furuya, Tomoko Yamamoto, Shinji Shimada

1258 related Products with: A first Case of Meningitis/Encephalitis associated with SARS-Coronavirus-2.

1mg200 1mg300 100 ug/vial100 100 µg200 300 1mg100 100 µg

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#32251780   2020/04/03 To Up

Modified bacterial outer membrane vesicles induce autoantibodies for tumor therapy.

Using monoclonal antibodies to block tumor angiogenesis has yielded effective antitumor effects. However, this treatment method has long cycles and is very expensive; therefore, its long-term and extensive application is limited. In this study, we developed a nanovaccine using bacterial biomembranes as carriers for antitumor therapy. The whole basic fibroblast growth factor (BFGF) molecule (154 amino acids (aa)) was loaded onto bacterial outer membrane vesicles (OMVs) using gene recombination technology. The strong adjuvant effect of OMVs was used to induce the host to produce anti-BFGF autoantibodies. We proved that persistent anti-BFGF autoantibodies can be induced in mice after only 3 immunizations to antagonize BFGF functions. The effects included multiple tumor suppression functions, including inhibition of tumor angiogenesis, induction of tumor cell apoptosis, reversal of tumor immune barriers, and promotion of tumor-specific cytotoxic T lymphocytes (CTLs), eventually causing tumor regression. We confirmed that bacterial biomembranes can be used as a vaccine delivery system to induce the production of antibodies against autoantigens, which may be used for tumor therapy. This study expands the application fields of bacterial biomembrane systems and provides insight for tumor immunotherapy other than monoclonal antibody technology.
Weiwei Huang, Congyan Shu, Liangqun Hua, Yilin Zhao, Hanghang Xie, Jialong Qi, Fulan Gao, Ruiyu Gao, Yongjun Chen, Qishu Zhang, Weiran Li, Mingcui Yuan, Chao Ye, Yanbing Ma

2144 related Products with: Modified bacterial outer membrane vesicles induce autoantibodies for tumor therapy.

0.1 ml0.2 mg100ug96T 100 Slides 0,14 Sample Kit2

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#32251706   2020/04/03 To Up

Evolving functions of Dickkopf-1 in cancer and immunity.

Dickkopf-1 (DKK-1) is a well-established inhibitor of canonical Wnt-signaling that critically participates in the regulation of bone formation and has been implicated in the development and progression of bone metastases. While the skeleton was originally considered the sole site of DKK-1 synthesis, it has now become clear that the molecule is also highly expressed in T-cells, platelets and multiple cancer cells. In the past years, several new functions of DKK-1 in angiogenesis, cancer cell biology, immune homeostasis and inflammation have been revealed. These novel insights have paved the way for clinical trials investigating the efficacy of anti-DKK-1 antibodies in a variety of different malignancies, most of which are currently still ongoing. In this review, we discuss the evolution and recent advances in DKK-1 research and highlight clinical implications of the available knowledge on the molecule, especially in cancer. Finally, we emphasize outstanding questions and provide an outlook on potential future studies that will aid in further improving our understanding of the pleiotropic roles of DKK-1 in health and disease.
Nikolai Jaschke, Lorenz C Hofbauer, Andy Göbel, Tilman D Rachner

1630 related Products with: Evolving functions of Dickkopf-1 in cancer and immunity.

100ug1000 TESTS/0.65ml100ug Lyophilized250 MG 100ul100ug100ug100ug Lyophilized100ug

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#32251641   2020/04/03 To Up

Safety and immunogenicity of a parenteral trivalent P2-VP8 subunit rotavirus vaccine: a multisite, randomised, double-blind, placebo-controlled trial.

A monovalent, parenteral, subunit rotavirus vaccine was well tolerated and immunogenic in adults in the USA and in toddlers and infants in South Africa, but elicited poor responses against heterotypic rotavirus strains. We aimed to evaluate safety and immunogenicity of a trivalent vaccine formulation (P2-VP8-P[4],[6],[8]).
Michelle J Groome, Lee Fairlie, Julie Morrison, Alan Fix, Anthonet Koen, Maysseb Masenya, Lisa Jose, Shabir A Madhi, Nicola Page, Monica McNeal, Len Dally, Iksung Cho, Maureen Power, Jorge Flores, Stanley Cryz

2147 related Products with: Safety and immunogenicity of a parenteral trivalent P2-VP8 subunit rotavirus vaccine: a multisite, randomised, double-blind, placebo-controlled trial.

100ug96 wells (1 kit)1 ml100 ul1 mg5mg1 mg1 g 100ul 100ul100μg

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