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#33608654   2021/02/19 To Up

The effect of oat β-glucan on postprandial blood glucose and insulin responses: a systematic review and meta-analysis.

To determine the effect of oat β‑glucan (OBG) on acute glucose and insulin responses and identify significant effect modifiers we searched the MEDLINE, EMBASE, and Cochrane databases through October 27, 2020 for acute, crossover, controlled feeding trials investigating the effect of adding OBG (concentrate or oat-bran) to carbohydrate-containing test-meals compared to comparable or different carbohydrate-matched control-meals in humans regardless of health status. The primary outcome was glucose incremental area-under-the-curve (iAUC). Secondary outcomes were insulin iAUC, and glucose and insulin incremental peak-rise (iPeak). Two reviewers extracted the data and assessed risk-of-bias and certainty-of-evidence (GRADE). Data were pooled using generic inverse-variance with random-effects model and expressed as ratio-of-means with [95% CIs]. We included 103 trial comparisons (N = 538). OBG reduced glucose iAUC and iPeak by 23% (0.77 [0.74, 0.81]) and 28% (0.72 [0.64, 0.76]) and insulin by 22% (0.78 [0.72, 0.85]) and 24% (0.76 [0.65, 0.88]), respectively. Dose, molecular-weight, and comparator were significant effect modifiers of glucose iAUC and iPeak. Significant linear dose-response relationships were observed for all outcomes. OBG molecular-weight >300 kg/mol significantly reduced glucose iAUC and iPeak, whereas molecular-weight <300 kg/mol did not. Reductions in glucose iAUC (27 vs 20%, p = 0.03) and iPeak (39 vs 25%, p < 0.01) were significantly larger with different vs comparable control-meals. Outcomes were similar in participants with and without diabetes. All outcomes had high certainty-of-evidence. In conclusion, current evidence indicates that adding OBG to carbohydrate-containing meals reduces glycaemic and insulinaemic responses. However, the magnitude of glucose reduction depends on OBG dose, molecular-weight, and the comparator.
Andreea Zurbau, Jarvis C Noronha, Tauseef A Khan, John L Sievenpiper, Thomas M S Wolever

2282 related Products with: The effect of oat β-glucan on postprandial blood glucose and insulin responses: a systematic review and meta-analysis.

10 mg100 mg100ul200ul25 mg100 mg 25 MG50 ug 1 ml100ug10 mg

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#33576436   2021/02/12 To Up

Structural analysis and macrophage activation of a novel β‑glucan isolated from .

The present study was designed to investigate the structure and immunomodulatory activity of a polysaccharide. A novel acidic β‑glucan (WCCP‑A‑b; molecular weight, 7.3 kDa) was purified from the fruiting bodies of the edible mushroom , which possesses high nutritional values. WCCP‑A‑b was composed primarily of glucose (89.7%) and glucuronic acid (8.8%). Methylation and nuclear magnetic resonance analysis suggested that WCCP‑A‑b contained β‑D‑1,6‑glucan as its main chain, which was substituted at O‑3 by β‑1,3‑D‑Glc oligosaccharides or a single‑unit of β‑Glc residues. Minor β‑1,4‑D‑GlcA residues may also be present in the side chains. The degree of branching was ~20.9%. Moreover, WCCP‑A‑b possessed a macrophage activating effect by promoting the secretion of nitric oxide, TNF‑α and IL‑6 in a dose‑dependent manner. At a cellular mechanistic level, WCCP‑A‑b activated macrophages via the MAPK signaling pathway. The present results provided useful information for supporting further investigations on the structure‑activity association of polysaccharides from , and indicated that the novel β‑glucan may be a potent natural immunomodulator, thus promoting the application of as a valuable source for functional food.
Yunhe Qu, Xiaolin Zhao, Huijun Guo, Yue Meng, Yumeng Wang, Yifa Zhou, Lin Sun

1084 related Products with: Structural analysis and macrophage activation of a novel β‑glucan isolated from .

1 module10 mg 15 ml 1 module1 module50 1000 tests2ug100ug1 module2ug

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#31337356   2019/07/23 To Up

Serum-based diagnosis of Pneumocystis pneumonia by detection of Pneumocystis jirovecii DNA and 1,3-β-D-glucan in HIV-infected patients: a retrospective case control study.

Pneumocystis jirovecii pneumonia (PCP) is one of the most common HIV-related opportunistic infections. The diagnosis of PCP is based on analyses from respiratory tract specimens which may require the invasive procedure of a diagnostic bronchoscopy. The objective of this study was to evaluate the diagnostic potential of Pneumocystis jirovecii PCR in serum combined with the 1,3-β-D-glucan (betaglucan) test for the diagnosis of PCP in HIV-infected patients.
Helena Hammarström, Anna Grankvist, Isabell Broman, Nahid Kondori, Christine Wennerås, Magnus Gisslen, Vanda Friman

2537 related Products with: Serum-based diagnosis of Pneumocystis pneumonia by detection of Pneumocystis jirovecii DNA and 1,3-β-D-glucan in HIV-infected patients: a retrospective case control study.



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#30447357   2018/11/15 To Up

Development of photocrosslinked salecan composite hydrogel embedding titanium carbide nanoparticles as cell scaffold.

Salecan is a water-soluble extracellular β‑glucan and appropriate for preparing hydrogels applicable in biomedical field. Herein, an innovative photocrosslinked composite hydrogel composed of salecan/poly(hydroxypropyl methacrylate (PHPMA) network and titanium carbide (TiC) nanoparticles was successfully prepared. XRD patterns clearly showed the crystal planes of Ti. More importantly, the introduction of TiC nanoparticles into the hydrogel network provided structural reinforcement, and thereby synergistically enhanced the mechanical strength and thermal stability of the hydrogels. The change of salecan content markedly affected the swelling behavior and morphology of the composite hydrogels. Cytotoxicity results revealed that the composite hydrogels were non-toxic to L929 and 3T3L1 cells. Cell proliferation and Live/Dead assay further demonstrated excellent cytocompatibility of the cells within the composite hydrogels, which highlights the value of this system for potential application as soft tissue engineering scaffold.
Xinyu Hu, Yongmei Wang, Man Xu, Liangliang Zhang, Jianfa Zhang, Wei Dong

2903 related Products with: Development of photocrosslinked salecan composite hydrogel embedding titanium carbide nanoparticles as cell scaffold.

1 kit100 Tests100002500 Tests500 assays1 kit24 tests1 kit10 plates1 kit500

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#30431070   2018/11/09 To Up

β‑glucan, a dectin‑1 ligand, promotes macrophage M1 polarization via NF‑κB/autophagy pathway.

Pro‑inflammatory (M1) macrophages have key roles in atherogenesis. As β‑glucan has been demonstrated to exert pro‑inflammatory effects, the present study examined whether β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype. The results from reverse transcription‑quantitative polymerase chain reaction, flow cytometry, western blotting and transmission electron microscope indicated that M1 macrophage markers inducible nitric oxide synthase and cluster of differentiation 80 were upregulated, dectin‑1 (a receptor for β‑glucan) expression and nuclear factor (NF)‑κB nuclear translocation were promoted, and autophagy level was inhibited following β‑glucan treatment of macrophages. Additionally, dectin‑1 small interfering RNA (siRNA), autophagy inducer rapamycin and NF‑κB inhibitor SN50 reversed the effects of β‑glucan on autophagy level and macrophage M1 polarization, suggesting that dectin‑1 and NF‑κB are upstream of autophagy in β‑glucan‑induced macrophage M1 polarization. Notably, simultaneous treatment with dectin‑1 siRNA and SN50 exhibited similar effects on β‑glucan‑reduced autophagy compared with dectin‑1 siRNA treatment alone. These findings demonstrate that dectin‑1 may mediate β‑glucan‑reduced autophagy through NF‑κB in macrophages. Accordingly, results from hematoxylin and eosin staining, western blotting and immunofluorescence staining demonstrated that β‑glucan accelerated the progress of atherosclerosis in apolipoprotein E‑deficient mice and modulated expression of dectin‑1, beclin‑1 and light chain 3II/I in aortas similarly to that observed in macrophages. These results indicate that dectin‑1 activation by β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype in an NF‑κB‑autophagy‑dependent pathway.
Xiuying Li, Hongli Luo, Yun Ye, Xuan Chen, Yuhong Zou, Jie Duan, Dong Xiang

1784 related Products with: β‑glucan, a dectin‑1 ligand, promotes macrophage M1 polarization via NF‑κB/autophagy pathway.

100010μg/vial100ug/vial1000 1000pcs10μg/vial100ug/vial100ug/vial1000 tests100ug/vial1000pcs100ug/vial

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#30412759   2018/11/06 To Up

Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.

Pure yeast β‑glucan (YG1) was obtained by drying and defatting the crude β‑glucan from Saccharomyces cerevisiae. The YG1 structure was characterized by total sugar content, protein content, FT-IR spectroscopy and monosaccharide composition analysis. Different molecular weight fractions of water-soluble yeast β‑glucan (WYG) were prepared by extraction with 2.0 M NaOH, degradation of the insoluble residue with 1.0 M HCl based on single-factor experiments, and fractionation on a size exclusion chromatography column (SEC, Sephacryl S-400). The molecular sizes of as-obtained fractions were measured by multi-angle laser light scattering combined with SEC and differential refractive index detector (SEC-MALLS-RI). Results indicated that YG1 had a high purity and was almost composed of β‑d‑glucose (97.71%) except trace mannose. The WYG yields by alkali extraction and acidolysis were 12.41% and 42.85%, respectively. Fourteen fractions with molecular weight (M) from 4590 to 31.61 kDa and low polydispersity index (M/M of ~1) were successfully separated, showing high recovery rates of 61.9-92.5%. Additionally, these fractions could promote the proliferation of RAW264.7 macrophages, and the fraction (M = 2496 kDa) exhibited the highest cell viability of 145.8 ± 4.3% at a low concentration of 1.56 μg/mL. This work not only provides an efficient method for separating WYG fractions with different molecular weights and low polydispersity, but also lays a theoretical basis for interpreting the relationship between molecular size and bioactivity.
Zhaomin Zheng, Qilin Huang, Chuqi Ling

1154 related Products with: Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity.

10 ug50 ug20 ug10 mg 5 G2.5 mg1,000 tests100ul1 mg1 mg1000 100tests

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#30412757   2018/11/06 To Up

Polymeric nanoengineered HBsAg DNA vaccine designed in combination with β‑glucan.

Antigen-specific immune responses following DNA vaccination are hard to achieve, owing to the difficulty to mediate efficient gene delivery. This study proposed the use of PDMAEMA:PβAE/DNA polyplexes (Pol) as the vehicle of a pDNA vaccine encoding the hepatitis B surface antigen (HBsAg), with these Pol designed in combination with a soluble (Glu) or a particulate (GPs) form of β‑glucan. β‑Glucans are recognized adjuvants that activate immune cells, a good strategy to improve transfection efficiency and vaccine efficacy. Results showed that Pol produced at a 19:1 polymer:DNA (+/-) charge ratio were positively charged (+41 mV), had a mean size of 180 nm and presented high stability under different storage conditions. These polyplexes resulted in enhanced transfection activity than the positive control, showing even higher luciferase gene expression in the presence of GPs (COS-7 and RAW 264.7 cell lines). Additionally, no alterations in hemolysis and plasma coagulation time of human blood were found in the non-cytotoxic working range. Mice vaccination studies (pCMV-S), resulted in a seroconversion rate of 40%, regardless of the additional β‑glucan adjuvants. This work showed the potential of this nanosystem together with GPs to enhance in vitro transfection capacity and to be further studied as a DNA vaccination platform.
Edna Soares, Rosemeyre Cordeiro, Henrique Faneca, Olga Borges

1934 related Products with: Polymeric nanoengineered HBsAg DNA vaccine designed in combination with β‑glucan.

500 tests100 μg100 μg100ug20 100 μg100ug Lyophilized

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#28895878   2017/09/12 To Up

Effect of Oral Pre-Meal Administration of Betaglucans on Glycaemic Control and Variability in Subjects with Type 1 Diabetes.

We conducted a double-blind placebo-controlled crossover pilot study to investigate the effect of oat betaglucans (β-glucan) on glycaemic control and variability in adults with type 1 diabetes (T1D; = 14). Stomacol tablets (1.53 g of β-glucan) or placebo (Plac) were administered three times daily before meals for two weeks. Glucose levels were monitored during the second week by continuous glucose monitoring (CGM). There was an increase in basic measures of glycaemic control (maximal glucose value 341 ± 15 vs. 378 ± 13 mg/dL for Plac and β-glucan, = 0.004), and average daily risk range (62 ± 5 vs. 79 ± 4 mg/dL for Plac and β-glucan, = 0.003) favouring Plac over β-glucan, but no increase in the M-value (the weighted average of the glucose values) or other more complex measures. Basic measures of glucose variability were also slightly increased during β-glucan treatment, with no difference in more complex measures. However, glycaemic variability increased between the first and last two CGM days on Plac, but remained unchanged on β-glucan. In conclusion, in this pilot study we were unable to demonstrate a general positive effect of β-glucan before meals on glucose control or variability in T1D.
Anders Frid, Andrea Tura, Giovanni Pacini, Martin Ridderstråle

1136 related Products with: Effect of Oral Pre-Meal Administration of Betaglucans on Glycaemic Control and Variability in Subjects with Type 1 Diabetes.

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#28606531   2017/05/20 To Up

All-natural bio-plastics using starch-betaglucan composites.

Grain polysaccharides represent potential valuable raw materials for next-generation advanced and environmentally friendly plastics. Thermoplastic starch (TPS) is processed using conventional plastic technology, such as casting, extrusion, and molding. However, to adapt the starch to specific functionalities chemical modifications or blending with synthetic polymers, such as polycaprolactone are required (e.g. Mater-Bi). As an alternative, all-natural and compostable bio-plastics can be produced by blending starch with other polysaccharides. In this study, we used a maize starch (ST) and an oat β-glucan (BG) composite system to produce bio-plastic prototype films. To optimize performing conditions, we investigated the full range of ST:BG ratios for the casting (100:0, 75:25, 50:50, 25:75 and 0:100 BG). The plasticizer used was glycerol. Electron Paramagnetic Resonance (EPR), using TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) as a spin probe, showed that the composite films with high BG content had a flexible chemical environment. They showed decreased brittleness and improved cohesiveness with high stress and strain values at the break. Wide-angle X-ray diffraction displayed a decrease in crystallinity at high BG content. Our data show that the blending of starch with other natural polysaccharides is a noteworthy path to improve the functionality of all-natural polysaccharide bio-plastics systems.
Domenico Sagnelli, Jacob J K Kirkensgaard, Concetta Valeria L Giosafatto, Natalia Ogrodowicz, Krzysztof Kruczała, Mette S Mikkelsen, Jean-Eudes Maigret, Denis Lourdin, Kell Mortensen, Andreas Blennow

1721 related Products with: All-natural bio-plastics using starch-betaglucan composites.

11 mg1 kg0.1 mg1 kg96T100 assays25 Plates/Unit10025 200

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