Search results for: Rabbit Anti-MER+SKY Polyclonal Antibody, Gold conjugated Isotype: IgG
#32663533 2020/07/11 To Up
A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.
BRamasamy Selvarajan, Prasanya Selvam Kanichelvam, Velusamy Balasubramanian, Sundaram Sethurama Subramanian
2373 related Products with: A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.
96 tests100tests1 kit100tests25 96 Tests 24 tests1 kitRelated Pathways
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#32199081 2020/02/24 To Up
Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
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1364 related Products with: Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).
One 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki500 testsOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiRelated Pathways
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#28727765 2017/07/20 To Up
Defining the target and the effect of imatinib on the filarial c-Abl homologue.
Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites.Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman
2987 related Products with: Defining the target and the effect of imatinib on the filarial c-Abl homologue.
min 2 cartons100.00 ul1 ml1200 units1500 Units1Related Pathways
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#19699744 2009/08/21 To Up
Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.
For the application of antibodies in rapid test systems such as Lateral Flow Devices (LFD) antibodies have to be coupled to coloured particles for immediate readability of the test system. In this work colloidal gold was selected for conjugation to the antibodies. Polyclonal rabbit antibodies were chosen for the development of Lateral Flow Devices for the detection of bovine alpha-casein. For antibody comparison chicken egg yolk IgY and sheep IgG were additionally used. Rabbit and chicken antibodies were purified from rabbit sera and egg yolk using affinity chromatography and alternatively ammonium sulphate precipitation, Sheep IgG was commercially obtained. In the course of colloidal gold sol titration experiments differences not only between antibody species but also between differently purified rabbit IgG were observed. While affinity purified rabbit IgG was not able to stabilise colloidal gold particles, antibodies obtained by ammonium sulphate precipitation resulted in a stable gold conjugate suitable for application in Lateral Flow Assays. This work compares and discusses the impact of antibody pre-treatment on further conjugation capacity.Judith Rudolf, Manuela Führer, Brigitte Galler, Parisa Ansari, Christoph Hasenhindl, Sabine Baumgartner
1929 related Products with: Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.
100ul100ug Lyophilized50 ug 1000 100ug Lyophilized1 ml1000 TESTS/0.65ml50 ug 100ug Lyophilized100ug100.00 ul50 ugRelated Pathways
#10583677 // To Up
Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.
Specific nisin polyclonal antibodies (PAb) were produced in rabbits using nisin Z produced by Lactococcus lactis subsp. lactis biovar diacetylactis UL 719. Antisera were obtained from white female New Zealand rabbits that were first immunized with a nisin Z-keyhole limpet haemocyanin conjugate and boosted with free nisin Z. Nisin-specific PAb were purified by affinity chromatography with a yield of 15 mg specific antinisin 100 ml-1 serum. The detection limit of the ELISA test for nisin Z was 0.75 ng ml-1 in buffer but was 1.7 and 3.5 ng ml-1 in milk and complex media broth spiked (5, 10, 20 microg ml-1) with nisin Z, respectively. In nisin Z-spiked samples, the average concentration was between 90 and 107% of actual added amount. In contrast, when the bioassay (microtitration method) was used, only 50-63% of nisin Z biological activity could be detected. In addition, the affinity-purified nisin PAb, antirabbit IgG gold conjugate and transmission electron microscopy were successfully used to locate nisin Z on producing cells and to observe its bactericidal effects against sensitive cells.M Bouksaim, C Lacroix, R Bazin, R E Simard
2111 related Products with: Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.
100ug Lyophilized50 ug 50 ug 96T50 ug 100ug Lyophilized100ug Lyophilized96 wells (1 kit)1000 TESTS/0.65ml0.1 mg1 ml0.1ml (1mg/ml)Related Pathways
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