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Search results for: Mouse Anti-Beta-2-MG(B2E5) Monoclonal Antibody, Gold Conjugated

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#35715914   // To Up

Photothermolysis Mediated by Gold Nanorods Conjugated with Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody Induces Apoptosis via the Mitochondrial Apoptosis Pathway in Laryngeal Squamous Cell Cancer.

Gold nanorods (AuNRs) have unique optical properties and biological affinity and can be used to treat tumors when conjugated with other protein molecules. Our previous studies have shown that EGFR monoclonal antibody (EGFRmAb)-modified AuNRs exert strong antitumor activity by inducing apoptosis. In this study, we tested the effects of EGFRmAb-modified AuNRs on laryngeal squamous cell cancer (LSCC) and . The results showed that EGFRmAb-modified AuNRs inhibited NP-69, BEAS-2B and Hep-2 cell growth and induced mitochondria-dependent apoptosis. The mitochondrial membrane potential was reduced, leading to the release of cytochrome C (Cyt C) and consequent activation of the intrinsic mitochondrial apoptosis pathway. Moreover, we observed that the occurrence of mitochondrial apoptosis is related to the destruction of the lysosome-mitochondria axis. To verify the effects , we also established a laryngeal tumor model in nude mice by subcutaneous transplantation. In model mice treated with EGFRmAb-modified AuNRs and irradiated with an NIR laser, tumor cell apoptosis and tumor growth were inhibited. These results suggest that EGFRmAb-modified AuNRs induced apoptosis through the intrinsic mitochondrial apoptotic pathway and are a potential candidate for cancer therapy.
Shi-Wen Zhang, Hao Wang, You-Yu Qiu, Ren-Chao Huang, Zi-Chen Dong, Lu Zhang, Liu-Fang Zhao, Hong-Yang Xu, Wei-Di Sun

1957 related Products with: Photothermolysis Mediated by Gold Nanorods Conjugated with Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody Induces Apoptosis via the Mitochondrial Apoptosis Pathway in Laryngeal Squamous Cell Cancer.

100ug100 ug2 Pieces/Box2 Pieces/Box2 Pieces/Box100ug Lyophilized100ul100 ug100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized

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#35355485   // To Up

[Development of a colloidal gold based immunochromatographic strip for 8-OHdG detection].

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive and stable biomarker for evaluating DNA oxidative damage. A rapid and sensitive colloidal gold immunochromatographic strip was developed for 8-OHdG detection by a competitive method. The sample pad (glass cellulose film), bonding pad (glass cellulose film), nitrocellulose film and absorbent pad were pasted on the polyvinyl chloride (PVC) base plate to construct the test strip. Colloidal gold (AuNPs) was prepared by the reduction of chloroauric acid with sodium citrate. 8-OHdG antibody (Ab) was coated on the outer layer of AuNPs to form [email protected] as a probe. Bovine serum albumin (BSA) and 8-OHdG were conjugated with carbodiimide hydrochloride to prepare an artificial antigen, which was used as the coating antigen of detection line. Goat anti mouse polyclonal antibody IgG was used as the coating antibody of control line. The experimental parameters were optimized including the type of nitrocellulose membrane, the formula of loading solution, and the spraying amount of gold labeled antibody. The results showed that the appropriate nitrocellulose membrane was CN 95. The optimal loading solution included BSA (1%), Tween-20 (3%), sucrose (3%) and NaCl (0.9%). The optimal spraying amount of gold labeled antibody was 4 μL. 8-OHdG can be detected by the strip under visible light, and the level of 8-OHdG in urine can be preliminarily determined by comparing the color intensity of T line and C line. The 8-OHdG concentration in urine was further calculated by the gray value of T line and the threshold of detection was 2.55 μg/L. This colloidal gold immunochromatographic strip is simple, rapid and specific for detecting 8-OHdG in human urine to preliminarily evaluate the human status.
Weiwei Ye, Liwen Wang, Yu Zhang, Chaofeng Li, Tianrun Qian, Xianshu Fu, Mingzhou Zhang, Jihong Shan

1439 related Products with: [Development of a colloidal gold based immunochromatographic strip for 8-OHdG detection].

100ug100ug100ug Lyophilized0.1 mg100ug Lyophilized100ug100ug Lyophilized100ug LyophilizedOne 96-Well Strip Micropl100ug100ug

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#35266934   2022/03/24 To Up

Sensitive detection of the okadaic acid marine toxin in shellfish by [email protected] NPs/horseradish peroxidase dual catalysis immunoassay.

Based on the catalysis enhancement strategy of [email protected] nanoparticles ([email protected] NPs) and horseradish peroxidase (HRP) related to the TMB-HO indicator, a sensitive colorimetric immunoassay was established for trace okadaic acid (OA) detection. The anti-OA monoclonal antibody (McAb) with a high constant was prepared and modified on [email protected] NPs. Through grafting the HRP conjugated goat anti-mouse IgG antibody (IgG) on [email protected]/McAb, bifunctional composites with [email protected] and HRP were prepared and adopted. Characteristics including morphology, specificity and catalytic performance were evaluated. Under the optimal conditions, the sensitivity of the resultant enzyme immunoassay was significantly improved, and a low limit of detection (LOD) of OA was achieved at 0.04 ng mL (equivalent to 0.6 μg kg in mussel tissue), which was better than that of most HRP or Au/HRP enzyme-linked immunosorbent assays. When applied to fortified shellfish samples ( oysters, mussels and clams), the recoveries ranging from 98.3 ± 2.3% to 106.0 ± 9.0% were acceptable and comparable with those of the LC-MS method. Acceptable precision was achieved with a variation coefficient (CV) of 2.3-8.4%. The method provides a promising alternative for the highly sensitive detection of the OA marine toxin at trace levels.
Yinqi Tian, Lin Yuan, Min Zhang, Youfen He, Xucong Lin

1242 related Products with: Sensitive detection of the okadaic acid marine toxin in shellfish by [email protected] NPs/horseradish peroxidase dual catalysis immunoassay.

100ug100tests100 μg50 ul100ug Lyophilized 125 ml 100 μg1 Set1 Set

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#34979402   2021/12/23 To Up

Expanded detection range of lateral flow immunoassay endowed with a third-stage amplifier indirect probe.

Here, a third-stage amplifier indirect probe (TsAIP) based lateral flow immunoassay (LFIA) was proposed to detect furazolidone (FZD) with Prussian blue nanoparticles (PBNPs) as carrier to label the goat anti-mouse antibody-horseradish peroxidase conjugation [GAMA(HRP)]. In this strategy, owing to the fact that one monoclonal antibody (mAb) can combine several GAMA molecules simultaneously, the indirect probe can generate primary signal amplification, then realize second-stage amplification attributing to PBNPs, and finally achieve third-stage amplification because of the conjugated HRP. The TsAIP-based LFIA shows improved performance for FZD metabolite derivative with a detection limit of 1 ng mL. The detection range is expanded about 2-fold compared with the original outcome. Besides, the proposed sensor could be successfully applied in food samples. This method provides a platform for broadening the detection range and application of PBNPs based LFIAs.
Jing Ren, Lihong Su, Huilan Hu, Xuechi Yin, Jingke Xu, Sijie Liu, Jianlong Wang, Zhanhui Wang, Daohong Zhang

1644 related Products with: Expanded detection range of lateral flow immunoassay endowed with a third-stage amplifier indirect probe.

1 kit100ug Lyophilized100ug Lyophilized100ug Lyophilized1 kit100ug Lyophilized

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#34543643   2021/09/20 To Up

Generation and characterisation of a semi-synthetic siderophore-immunogen conjugate and a derivative recombinant triacetylfusarinine C-specific monoclonal antibody with fungal diagnostic application.

Invasive pulmonary aspergillosis (IPA) is a severe life-threatening condition. Diagnosis of fungal disease in general, and especially that caused by Aspergillus fumigatus is problematic. A. fumigatus secretes siderophores to acquire iron during infection, which are also essential for virulence. We describe the chemoacetylation of ferrated fusarinine C to diacetylated fusarinine C (DAFC), followed by protein conjugation, which facilitated triacetylfusarinine C (TAFC)-specific monoclonal antibody production with specific recognition of the ferrated form of TAFC. A single monoclonal antibody sequence was ultimately elucidated by a combinatorial strategy involving protein LC-MS/MS, cDNA sequencing and RNAseq. The resultant murine IgG2 monoclonal antibody was secreted in, and purified from, mammalian cell culture (5 mg) and demonstrated to be highly specific for TAFC detection by competitive ELISA (detection limit: 15 nM) and in a lateral flow test system (detection limit: 3 ng), using gold nanoparticle conjugated- DAFC-bovine serum albumin for competition. Overall, this work reveals for the first time a recombinant TAFC-specific monoclonal antibody with diagnostic potential for IPA diagnosis in traditional and emerging patient groups (e.g., COVID-19) and presents a useful strategy for murine Ig sequence determination, and expression in HEK293 cells, to overcome unexpected limitations associated with aberrant or deficient murine monoclonal antibody production.
Nicola M Moloney, Annemarie Larkin, Linan Xu, David A Fitzpatrick, Holly L Crean, Kieran Walshe, Hubertus Haas, Clemens Decristoforo, Sean Doyle

2069 related Products with: Generation and characterisation of a semi-synthetic siderophore-immunogen conjugate and a derivative recombinant triacetylfusarinine C-specific monoclonal antibody with fungal diagnostic application.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#33945531   2021/05/04 To Up

An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin.

Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the detection of T. marneffei antigens in clinical urine specimens. The T. marneffei yeast phase specific monoclonal antibody 4D1 (MAb4D1) conjugated with colloidal gold nanoparticle was used as a specific signal reporter. Galanthus nivalis Agglutinin (GNA) was adsorbed onto nitrocellulose membrane to serve as the test line. Similarly, a control line was created above the test line by immobilization of rabbit anti-mouse IgG. The immobilized GNA served as capturing molecule and as non-immune mediated anti-terminal mannose of T. marneffei antigenic mannoprotein. The MAb4D1-GNA based ICT showed specific binding activity with yeast phase antigen of T. marneffei, and it did not react with other common pathogenic fungal antigens. The limit of detection of this ICT for T. marneffei antigen spiked in normal urine was approximately 0.6 μg/ml. The diagnostic performance of the ICT was validated using 341 urine samples from patents with culture- confirmed T. marneffei infection and from a control group of healthy individuals and patients with other infections in an endemic area. The ICT exhibited 89.47% sensitivity, 100% specificity, and 97.65% accuracy. Our results demonstrate that the urine-based GNA-MAb4D1 based ICT produces a visual result within 30 minutes and that the test is highly specific for the diagnosis of T. marneffei infection. The findings validate the deployment of the ICT for clinical use.
Kritsada Pruksaphon, Akarin Intaramat, Pavinee Simsiriwong, Skorn Mongkolsuk, Kavi Ratanabanangkoon, Joshua D Nosanchuk, Anna Kaltsas, Sirida Youngchim

1157 related Products with: An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin.

100 TESTS0.2 mg1 ml25 µg25 µg0.25 mg0.2 mg100.00 ug1000 tests1000 tests

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#33827632   2021/04/07 To Up

Development of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses.

H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection.
Ge Li, Xun Wang, Qingmei Li, Jifei Yang, Xiao Liu, Wenbao Qi, Junqing Guo, Ruiguang Deng, Gaiping Zhang

2644 related Products with: Development of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses.

100μg10 100 ul1mg1 ml100 assays1 mg100One 96-Well Strip Micropl

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#33787893   // To Up

Development of a Colloidal Gold Immunochromatographic Strip for the Rapid Detection of Channel Catfish Virus.

Channel catfish virus disease (CCVD) has resulted in great economic losses and has restricted the development of fisheries. There is therefore, a need for rapid and efficient diagnostic methods to control the spread of CCVD.
Hongli Jing, Xiaolin Li, Lipu Xu, Longying Gao, Xiangmei Lin, Min Zhang, Na Wang, Xiaofei Liu, Shaoqiang Wu

2062 related Products with: Development of a Colloidal Gold Immunochromatographic Strip for the Rapid Detection of Channel Catfish Virus.

0.25 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100.00 ul500 tests32-50 Sample Kit100ug100ug Lyophilized100ug Lyophilized0.25 mg

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#33624188   2021/02/24 To Up

EGFR-specific single-chain variable fragment antibody-conjugated FeO/Au nanoparticles as an active MRI contrast agent for NSCLC.

Overexpression of epidermal growth factor receptor (EGFR) is closely associated with a poor prognosis in non-small cell lung cancer (NSCLC), thus making it a promising biomarker for NSCLC diagnosis. Here, we conjugated a single-chain antibody (scFv) targeting EGFR with FeO/Au nanoparticles to form an EGFR-specific molecular MRI bioprobe ([email protected]/Au) to better detect EGFR-positive NSCLC tumors in vivo. In vitro, we demonstrated that the EGFR-specific scFv could specifically deliver FeO/Au to EGFR-positive NSCLC cells. In vivo experiments showed that the accumulation of [email protected]/Au in tumor tissue was detectable by magnetic resonance imaging (MRI) at the indicated time points after systemic injection. The T2W signal-to-noise ratio (SNR) of EGFR-positive SPC-A1 tumors was significantly decreased after [email protected]/Au injection, which was not observed in the tumors of mice injected with [email protected]/Au. Furthermore, transmission electron microscopy (TEM) analysis showed the specific localization of [email protected]/Au in the SPC-A1 tumor cell cytoplasm. Collectively, the results of our study demonstrated that [email protected]/Au might be a useful probe for the noninvasive diagnosis of EGFP-positive NSCLC.
Yuan Lu, Jing Huang, Fakai Li, Yuan Wang, Ming Ding, Jian Zhang, Hong Yin, Rui Zhang, Xinling Ren

2665 related Products with: EGFR-specific single-chain variable fragment antibody-conjugated FeO/Au nanoparticles as an active MRI contrast agent for NSCLC.

100 TESTS100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100 μg

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#33527460   2021/02/01 To Up

Development and evaluation of colloidal gold immunochromatography test strip for rapid diagnosis of nervous necrosis virus in golden grey mullet (Chelon aurata).

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Fatemeh Hassantabar, Mohammad J Zorriehzahra, Farid Firouzbakhsh, Kim D Thompson

1345 related Products with: Development and evaluation of colloidal gold immunochromatography test strip for rapid diagnosis of nervous necrosis virus in golden grey mullet (Chelon aurata).

20 ul50 ul100500 tests500 tests20 ul20 ul50 ul20 ul500 tests10050 ul

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