Only in Titles

Search results for: cell

paperclip

Error loading info... Pleas try again later.
paperclip

Error loading info... Pleas try again later.
paperclip

#32650338   2020/07/10 To Up

Structural basis for RIFIN-mediated activation of LILRB1 in malaria.

T
Thomas E Harrison, Alexander M Mørch, James H Felce, Akihito Sakoguchi, Adam J Reid, Hisashi Arase, Michael L Dustin, Matthew K Higgins

1459 related Products with: Structural basis for RIFIN-mediated activation of LILRB1 in malaria.

2 mg500 MG100μg 100 G100ug25 Bags/Unit250 mg 1 G100ug500 tests500 tests 1 G

Related Pathways

    No related Items
paperclip

#32650328   2020/07/10 To Up

Poly(ester amide) microspheres are efficient vehicles for long-term intracerebral growth factor delivery and improve functional recovery after stroke.

G
Tamar Memanishvili, Emanuela Monni, Jemal Tatarishvili, Olle Lindvall, Alexander Tsiskaridze, Zaal Kokaia, Daniel Tornero

1383 related Products with: Poly(ester amide) microspheres are efficient vehicles for long-term intracerebral growth factor delivery and improve functional recovery after stroke.

100μg0.1 mg20 ug5x10 ug10 mg2ug1 mg5 ml100.00 ug100 ug20 ug96 wells (1 kit)

Related Pathways

    No related Items
paperclip

#32650327   2020/07/10 To Up

Urn models for stochastic gene expression yield intuitive insights into the probability distributions of single-cell mRNA and protein counts.

F
Krishna Choudhary, Atul Narang

2146 related Products with: Urn models for stochastic gene expression yield intuitive insights into the probability distributions of single-cell mRNA and protein counts.

100ug100ug24 reactions 21 kit(96 Wells)100ug Lyophilized10100ug Lyophilized100

Related Pathways

    No related Items
paperclip

#32650326   2020/07/10 To Up

An integrated 3D-fluidic device with bubble guidance mechanism for long-term primary and secondary cell recordings on multielectrode array platform.

A 3D-fluidic device (3D-FD) is designed and developed with the capability of auto bubble guidance via a helical pathway in a 3D geometry. This assembly is integrated to a multielectrode array (MEA) to maintain secondary cell lines, primary cells and primary retinal tissue explants of chick embryos for continuous electrophysiology recording. The ability to maintain the retinal tissue explant, extracted from day 14 (E-14) and day 21 (E-21) chick embryos, in an integrated 3D-FD-MEA for long hours (> 100) is demonstrated. The enhanced duration of monitoring offered by this device is due to the controlled laminar flow and the maintenance of a stable microenvironment We could monitor the spontaneous electrical activity of the retina, including the spike recordings from the retinal ganglion layer in a sustained manner. Specifically, the spiking activity in embryonic chick retina of different days (E-14 to 21) is studied and the emergence of light stimulated firings along with a distinct electroretinogram (ERG) for E-21 mature-retina provides the evidence of a stable microenvironment over a sustained period.
Anil Krishna Konduri, C S Deepak, Sumukh Anil Purohit, K S Narayan

1741 related Products with: An integrated 3D-fluidic device with bubble guidance mechanism for long-term primary and secondary cell recordings on multielectrode array platform.

100ug0.1 mg100μg2 Pieces/Box

Related Pathways

paperclip

Error loading info... Pleas try again later.
paperclip

#32650324   2020/07/10 To Up

Biofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering.

The skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proves helpful in reducing culture time to create multilayered keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling.
Natan Barros, Hanjun Kim, Marcus J Goudie, KangJu Lee, Praveen Bandaru, Ethan A Banton, Einollah Sarikhani, Wujin Sun, Shiming Zhang, Hyun-Jong Cho, Martin C Hartel, Serge Ostrovidov, Samad Ahadian, Saber Hussain, Nureddin Ashammakhi, Mehmet R Dokmeci, Rondinelli Donizetti Herculano, Junmin Lee, Ali Khademhosseini

2271 related Products with: Biofabrication of endothelial cell, dermal fibroblast, and multilayered keratinocyte layers for skin tissue engineering.

50 assays

Related Pathways

paperclip

#32650322   2020/07/10 To Up

Non-random distribution of vacuoles in.

A central question in eukaryotic cell biology asks, during cell division, how is the growth and distribution of organelles regulated to ensure each daughter cell receives an appropriate amount. For vacuoles in budding yeast, there are well described organelle-to-cell size scaling trends as well as inheritance mechanisms involving highly coordinated movements. It is unclear whether such regulation is necessary in the symmetrically dividing fission yeast, Schizosaccharomyces pombe, in which random partitioning may be utilized to distribute vacuoles to daughter cells. To test this idea, we utilized machine learning to develop automated methods for segmenting fission yeast cells and locating vacuoles in live fission yeast cells from brightfield and fluorescence micrographs. We have found that the scaling trends of vacuole number, volume and surface area-to-cell size is consistent across a variety of strains, and that vacuole proliferation is a key mechanism for vacuole growth. We also show that vacuoles are not distributed evenly throughout the cell with respect to available cytoplasm. Rather, vacuoles show distinct peaks in distribution close to the nucleus, and this clustering was confirmed in mutants in which nucleus position is perturbed. Future work will establish the molecular mechanism for this clustering.
William Chadwick, Sujoy Biswas, Simone Bianco, Yee-Hung Mark Chan

1716 related Products with: Non-random distribution of vacuoles in.

25 ug 5 G

Related Pathways

paperclip

#32650321   2020/07/10 To Up

Recapitulating atherogenic flow disturbances and vascular inflammation in a perfusable 3D stenosis model.

Blood vessel narrowing and arterial occlusion are pathological hallmarks of atherosclerosis, which involves a complex interplay of perturbed hemodynamics, endothelial dysfunction and inflammatory cascade. Herein, we report a novel circular microfluidic stenosis model that recapitulates atherogenic flow-mediated endothelial dysfunction and blood-endothelial cell (EC) interactions in vitro. 2D and 3D stenosis microchannels with different constriction geometries were fabricated using 3D printing to study flow disturbances under varying severity of occlusion and wall shear stresses (100 to 2000 dynecm-2). Experimental and fluid simulation results confirmed the presence of pathological shear stresses in the stenosis region, and recirculation flow post stenosis. The resultant pathological flow profile induced pro-inflammatory and pro-thrombotic EC state as demonstrated by orthogonal EC alignment, enhanced platelet adhesion at the stenosis, and aberrant leukocyte-EC interactions post stenosis. Clinical utility of the vascular model was further investigated by testing anti-thrombotic and immunomodulatory efficacy of aspirin and metformin, respectively. Overall, the platform enables multi-factorial analysis of critical atherogenic events including endothelial dysfunction, platelets and leukocyte adhesion, and can be further developed into a liquid biopsy tool for cardiovascular risk stratification.
Nishanth Venugopal Menon, Chengxun Su, Kuin Tian Pang, Zhai Juan Phua, Hui Min Tay, Rinkoo Dalan, Xiaomeng Wang, Holden Li, Hanwei Hou

2588 related Products with: Recapitulating atherogenic flow disturbances and vascular inflammation in a perfusable 3D stenosis model.

8 Sample Kit4 Membranes/Box4 Sample Kit1-8 Sample Kit16 Arrays/Slide18 kgs4 Sample Kit

Related Pathways