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#33080603   2020/10/20 To Up

Lipoprotein and Metabolic Profiles Indicate Similar Cardiovascular Risk of Liver Steatosis and NASH.

Nonalcoholic fatty liver disease (NAFLD) affects about 25% of the global population, with no reliable noninvasive tests to diagnose nonalcoholic steatohepatitis (NASH) and to differentiate between NASH and nonalcoholic fatty liver (NAFL) (steatosis alone). It is unclear if NAFL and NASH differ in cardiovascular risk for patients. Here, we compared obese NAFLD patients with a healthy cohort to test whether cholesterol compounds could represent potential noninvasive markers and to estimate associated risks.
Aline Gottlieb, Anna-Sophia Leven, Jan-Peter Sowa, Katrin Borucki, Alexander Link, Elvan Yilmaz, Sitke Aygen, Ali Canbay, Mustafa Porsch-Özcürümez

2178 related Products with: Lipoprotein and Metabolic Profiles Indicate Similar Cardiovascular Risk of Liver Steatosis and NASH.

2.5 mg200ug10 mg 5 G1000 TESTS/0.65ml1,000 tests200 100ug1 mg100ul1000 tests

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#33080572   2020/10/20 To Up

Identification of a competing endogenous RNA axis related to gastric cancer.

Competing endogenous RNA (ceRNA) pathways play pivotal roles in the formation and progression of gastric cancer (GC). Employing multi-omics analysis, we sought to identify a ceRNA network associated with GC progression. We analyzed3Gene Expression Omnibus datasets as well as data from The Cancer Genome Atlas to identify genes that were differentially expressed in GC tissues. A total of 84 upregulated genes and 106 downregulated genes were found. Enrichment analysis indicated that some pathways were strongly linked with tumor formation and progression. We also screened hub genes to establish a lncRNA-miRNA-mRNA network. We ultimately identified 8 hub genes, 6 key miRNAs and 4 key lncRNAs that interact within a common ceRNA network. Correlation analysis and in vitro experiments were conducted to verify the regulatory effect of the ceRNA network in GC. A knockdown assay confirmed that the axis is a ceRNA network involved in GC progression. In this study, we elucidated the role of the ceRNA network in the progression of GC. These molecules maybe evaluated as therapeutic targets and prognostic biomarkers for GC.
Fuqiang Zu, Hezhou Han, Weiwei Sheng, Jian Sun, Hui Zang, Yu Liang, Qingfeng Liu

1217 related Products with: Identification of a competing endogenous RNA axis related to gastric cancer.

1 module2 Pieces/Box

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#33080570   2020/10/20 To Up

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2.

In this study, we investigated the functional and clinical significance of the long non-coding RNA (lncRNA) in human gliomas. TCGA, GTEx and CGGA database analyses showed that high expression correlates with advanced tumor stage and poor survival of glioma patients. expression is higher in glioma cell lines compared to normal human astrocytes (NHA). CCK-8, EdU, and colony formation assays show that knockdown decreases proliferation and colony formation in glioma cells, whereas, overexpression reverses these effects. Bioinformatics analysis showed that miR-129-5p is a potential target of MiR-129-5p expression negatively correlates with expression in glioma patients. Dual luciferase reporter assays confirmed that miR-129-5p binds directly to in glioma cells. RNA immunoprecipitation (RIP) assays using anti-Ago2 antibody pulled down with miR-129-5p. Bioinformatics analysis identified as a potential miR-129-5p target gene. Dual luciferase reporter assays confirmed that miR-129-5p binds directly to the 3'-UTR of 2 mRNA. Furthermore, miR-129-5p overexpression or knockdown reduces proliferation and colony formation of overexpressing glioma cells. These findings show that promotes gliomagenesis by enhancing ZRANB2 expression by sponging of miR-129-5p.
Xin Jiang, Dong Chen

2398 related Products with: LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2.

100ug10 mg 25UG1000 tests100ul100ug500 mg25 mg50 ug 10 mg 5 G100ul

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#33080554   2020/07/07 To Up

Gamma irradiation for enhancing active chemical compounds in leaf extracts of Libidibia ferrea (Leguminosae).

This research was designed to evaluate the influence of the irradiation process of the leaf extracts of Libidibia ferrea (Leguminosae) on the production of secondary chemical compounds, including their biological activity. Leaves were collected and prepared to obtain the crude extract, which was then aliquoted and separately exposed to a Co-60 source with different doses, namely: 5, 7, 10, 12, 15, 20, 25, and 30 kGy. From irradiated and control samples, tests of toxicity were carried out with the microcrustacea Artemia salina Leach at three moments: 24 h, 60 and 180 days after the irradiation of the samples. Bioassays showed an increase in the toxicity of the irradiated extracts, correlated with the dose. The toxicity level did not change with the storage time, indicating the excellent stability of the samples. To assess the phytochemical profile of the crude and irradiated extracts, three techniques were employed: thin-layer chromatography (TLC), liquid chromatography coupled to mass spectrometry (LC-MS), and gas chromatography coupled to mass spectrometry (GC-MS). The phytochemical results emphasized the presence of phenols, tannins, and triterpenes. The analytical tests confirmed the role of ionizing radiation in breaking down macromolecules into simpler chemical species responsible for increasing chemical activity of the extract. This report presents and discusses ionizing radiation as an outstanding tool for enhancing active chemical compounds in leaf extracts of Libidibia ferrea, which reflects on their biochemical properties.
Luiz da Silva Maia-Neto, Ademir Amaral, Luciano Rodolfo Ferreira de Lucena, Simey de Souza Leão Pereira Magnata, Edvane Borges da Silva, André Maciel Netto, Antônio Fernando Morais de Oliveira, Ivone Antônia de Souza

2482 related Products with: Gamma irradiation for enhancing active chemical compounds in leaf extracts of Libidibia ferrea (Leguminosae).

1 kit(96 Wells) 100 G20ug20ug100mg5 ug100.00 ug

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#33080548   2020/10/09 To Up

Differential diagnosis of benign and malignant male breast lesions in mammography.

To investigate the mammographic characteristics in discriminating benign and malignant male breast lesions.
Yan Huang, Qin Xiao, Yiqun Sun, Qin Li, Simin Wang, Yajia Gu

1714 related Products with: Differential diagnosis of benign and malignant male breast lesions in mammography.



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#33080547   2020/10/13 To Up

Antecedent Bile duct cutting in the glissonean pedicle technique (ABC technique) for extremely advanced perihilar cholangiocarcinoma.

In an increasing number of patients undergoing radical surgery for perihilar cholangiocarcinoma [1-3], the intrahepatic bile duct is conventionally transected after the vessels to be preserved or reconstructed are confirmed [3,4]. In patients with extremely advanced perihilar cholangiocarcinoma having massive vascular involvement, it is sometimes difficult to confirm the vessels for reconstruction because of restricted working space and/or anatomical variants, even after liver parenchymal dissection [4]. When the vessels cannot be confirmed, the tumor is usually unresectable [4].
Junichi Yoshikawa, Yutaro Kato, Atsushi Sugioka, Ichiro Uyama

2045 related Products with: Antecedent Bile duct cutting in the glissonean pedicle technique (ABC technique) for extremely advanced perihilar cholangiocarcinoma.

100 μg100 μg100 μg

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#33080536   2020/10/07 To Up

Toxicity assessment of a novel oil dispersant based on silica nanoparticles using Fathead minnow.

Oil spill accidents are a major concern for aquatic organisms. In recent history, the Deepwater Horizon blowout spilled 500 million liters of crude oil into the Gulf of Mexico. Corexit 9500A was used to disperse the oil since it was the method approved at that time, despite safety concerns about its use. A better solution is necessary for dispersing oil from spills that reduces the toxicity to exposed aquatic organisms. To address this challenge, novel engineered nanoparticles were designed using silica cores grafted with hyperbranched poly(glycidol) branches. Because the silica core and polymers are known to be biocompatible, we hypothesized that these particles are nontoxic to fathead minnows (Pimephales promelas) and would decrease their exposure to oil polyaromatic hydrocarbons. Fathead minnow embryos, juveniles and adult stages were exposed to the particles alone or in combination with a water-accommodated fraction of oil. Acute toxicity of nanoparticles to fish was tested by measuring mortality. Sub-lethal effects were also measured including gene expression of cytochrome P450 1a (cyp1a) mRNA and heart rate in embryos. In addition, a mixture of particles plus the water-accommodated fraction was directly introduced to adult female fathead minnows by gavage. Three different nanoparticle concentrations were used (2, 10, and 50 mg/L) in either artificial fresh water or the water-accommodated fraction of the oil. In addition, nanoparticle-free controls were carried out in the two solutions. No significant mortality was observed for any age group or nanoparticle concentration, suggesting the safety of the nanoparticles. In the presence of the water-accommodated fraction alone, juvenile and adult fathead minnows responded by increasing expression of cyp1a. The addition of nanoparticles to the water-accommodated fraction reduced cyp1a gene expression in treatments. Heart rate was also restored to normal parameters in embryos co-exposed to nanoparticles and to the water-accommodated fraction. Measurement of polyaromatic hydrocarbons confirmed their presence in the tested solutions and the reduction of available PAH in WAF treated with the nanoparticles. Our findings suggest the engineered nanoparticles may be protecting the fish by sequestering polyaromatic hydrocarbons from oil, measured indirectly by the induction of cypa1 mRNAs. Furthermore, chemical analysis showed a reduction in PAH content in the water accommodated fraction with the presence of nanoparticles.
Hajime Kurita-Oyamada, Caroline L Brown, Kevin J Kroll, Susan E Walley, Christopher Keller, Muhammad Ejaz, Marianne Kozuch, Wayne Reed, Scott Grayson, Daniel A Savin, Nancy D Denslow

2465 related Products with: Toxicity assessment of a novel oil dispersant based on silica nanoparticles using Fathead minnow.

500 testsOne 96-Well Microplate Ki1 mg0.2 mg100tests100.00 ug2.5 mg100ug0.2 mg

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