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#34563024   2021/09/10 To Up

Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties.

An artificial ovary is a promising approach for preserving fertility in prepubertal girls and women who cannot undergo current cryopreservation strategies. However, this approach is in its infancy, due to the possible challenges of creating a suitable 3D matrix for encapsulating ovarian follicles and stromal cells. To maintain the ovarian stromal cell viability and proliferation, as a first step towards developing an artificial ovary, in this study, a double network hydrogel with a high water swelling capacity (swelling index 15-19) was developed, based on phenol conjugated chitosan (Cs-Ph) and silk fibroin (SF) through an enzymatic crosslinking method using horseradish peroxidase. The addition of SF (1%) to Cs (1%) decreased the storage modulus (G') from 3500 Pa (Cs1) to 1600 Pa (Cs-SF1), and the hydrogels with a rapid gelation kinetic produced a spatially homogeneous distribution of ovarian cells that demonstrated 167% proliferation after 7 days. This new Cs-SF hydrogel benefits from the toughness and flexibility of SF, and phenolic chemistry could provide the potential microstructure for encapsulating human ovarian stromal cells.
Hafez Jafari, Arezoo Dadashzadeh, Saeid Moghassemi, Payam Zahedi, Christiani A Amorim, Amin Shavandi

2489 related Products with: Ovarian Cell Encapsulation in an Enzymatically Crosslinked Silk-Based Hydrogel with Tunable Mechanical Properties.

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#34562910   2021/09/07 To Up

A Variable Height Microfluidic Device for Multiplexed Immunoassay Analysis of Traumatic Brain Injury Biomarkers.

Traumatic brain injury (TBI) is a leading cause of global morbidity and mortality, partially due to the lack of sensitive diagnostic methods and efficacious therapies. Panels of protein biomarkers have been proposed as a way of diagnosing and monitoring TBI. To measure multiple TBI biomarkers simultaneously, we present a variable height microfluidic device consisting of a single channel that varies in height between the inlet and outlet and can passively multiplex bead-based immunoassays by trapping assay beads at the point where their diameter matches the channel height. We developed bead-based quantum dot-linked immunosorbent assays (QLISAs) for interleukin-6 (IL-6), glial fibrillary acidic protein (GFAP), and interleukin-8 (IL-8) using Dynabeads M-450, M-270, and MyOne, respectively. The IL-6 and GFAP QLISAs were successfully multiplexed using a variable height channel that ranged in height from ~7.6 µm at the inlet to ~2.1 µm at the outlet. The IL-6, GFAP, and IL-8 QLISAs were also multiplexed using a channel that ranged in height from ~6.3 µm at the inlet to ~0.9 µm at the outlet. Our system can keep pace with TBI biomarker discovery and validation, as additional protein biomarkers can be multiplexed simply by adding in antibody-conjugated beads of different diameters.
Alyse D Krausz, Frederick K Korley, Mark A Burns

1189 related Products with: A Variable Height Microfluidic Device for Multiplexed Immunoassay Analysis of Traumatic Brain Injury Biomarkers.

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#34562240   // To Up

Analysis of Protein Cholesterylation by Biorthogonal Labeling.

Cholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog and Smoothened, have been characterized to undergo cholesterylation. It is unknown whether other proteins are similarly modified. Here we present a protocol of a chemical biology method to analyze the Smoothened protein cholesterylation using an azido-conjugated cholesterol analog combined with click chemistry. This method can be applied to analyze Hedgehog cholesterylation, and be extended to identify novel cholesterylated proteins with minor changes.
Ao Hu, Meng Zhou, Bao-Liang Song

1883 related Products with: Analysis of Protein Cholesterylation by Biorthogonal Labeling.

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#34562050   2021/09/25 To Up

1,6-Anthrazoline-linked π-Conjugated Macrocycles and Two-dimensional Polymer via Friedländer Synthesis.

Synthesis of π-conjugated crystalline two-dimensional (2D) polymers remains largely unexplored due to limited synthetic methodology. Herein, we report the preparation of a 1,6-anthrazoline (AZ)-linked crystalline 2D polymer AZP via acid mediated Friedländer synthesis. The feasibility was examined first by two model reactions, followed by synthesis of three AZ-based macrocycles MCn (n=5-7), in which hexagonal MC6 was isolated as the major product. The favorable macrocycle formation could be largely attributed to the dynamic feature of Friedländer synthesis, which involves both imine condensation and aldol condensation. The structure and crystallinity of AZP were confirmed by experiments and simulation. The skeletons of the macrocycles and polymer consist of all-sp2 hybridized C/N atoms and are thus π-conjugated and electro-active. Our studies provide a rational way to access kinetically stable 2D crystalline polymers by combination of different dynamic covalent chemistries.
Jun Zhu, Shaofei Wu, Xudong Hou, Jishan Wu

2876 related Products with: 1,6-Anthrazoline-linked π-Conjugated Macrocycles and Two-dimensional Polymer via Friedländer Synthesis.

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#34560993   2021/08/21 To Up

N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.

N-trimethyl chitosan (TMC) is a multifunctional polymer that can be used in various nanoparticle forms in the pharmaceutical, nutraceutical and biomedical fields. In this study, TMC was used as a mucoadhesive adjuvant to enhance the oral bioavailability and hence antitumour effects of gemcitabine formulated into nanocomplexes composed of poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) conjugated with d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS). A central composite design was applied to achieve the optimal formulation. Cellular uptake and drug transportation studies revealed the nanocomplexes permeate over the intestinal cells via adsorptive-mediated and caveolae-mediated endocytosis. Pharmacokinetic studies demonstrated the oral drug bioavailability of the nanocomplexes was increased 5.1-fold compared with drug solution. In pharmacodynamic studies, the formulation reduced tumour size 3.1-fold compared with the drug solution. The data demonstrates that TMC modified nanocomplexes can enhance gemcitabine oral bioavailability and promote the anticancer efficacy.
Guanyu Chen, Darren Svirskis, Weiyue Lu, Man Ying, Hongyu Li, Min Liu, Jingyuan Wen

1360 related Products with: N-trimethyl chitosan coated nano-complexes enhance the oral bioavailability and chemotherapeutic effects of gemcitabine.

1 G10 mg96 Samples100μg 5 G 100 G200 10 mg125 extractions100 mg

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#34560561   2021/09/17 To Up

Development of bifunctional anti-PD-L1 antibody MMAE conjugate with cytotoxicity and immunostimulation.

In recent years, tumor immunotherapy, especially the combination of PD1/PD-L1 inhibitors and chemotherapy, has developed rapidly. However, the systemic side effects induced by chemotherapy remain a crucial problem that needs to be addressed. Antibody drug conjugates (ADCs) are exceptional target-specific prodrugs that greatly improve the therapeutic window of chemotherapy drugs. Therefore, designing PD-L1-targeting ADCs is an interesting research project. In this study, we confirmed for the first time that the commercial anti-PD-L1 antibody Atezolizumab has better endocytosis efficiencies than Avelumab, and was more suitable for ADC design. Then, the most popular cytotoxic payload MMAE was conjugated to Atezolizumab via a classical dipeptide (valine-alanine) linker to generate a bifunctional PD-L1 ADC (ADC 3). An in vitro cytotoxicity test indicated the potent tumor cell inhibitory activity of ADC 3, with EC values of 9.75 nM to 11.94 nM. In addition, a co-culture of PBMCs in vitro proved that ADC 3 retained the immune activation effect of the Atezolizumab antibody. Moreover, ADC 3 exhibited a higher tumor inhibition rate and tumor regression rate in humanized immune system mice. To the best of our knowledge, this is the most active PD-L1-ADC reported thus far, which may promote the development of immunotherapy and novel ADCs.
Dian Xiao, Longlong Luo, Jiaguo Li, Zhihong Wang, Lianqi Liu, Fei Xie, Jiannan Feng, Xinbo Zhou

2484 related Products with: Development of bifunctional anti-PD-L1 antibody MMAE conjugate with cytotoxicity and immunostimulation.

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#34560078   2021/09/21 To Up

Synthesis and biological activity of 1H-imidazo[4,5-f][1,10]phenanthroline as a potential antitumor agent with PI3K/AKT/mTOR signaling.

1H-imidazo[4,5-f][1,10]phenanthroline (IPM713) is a type of tricyclic conjugated rigid planar structure with potential medical applications, but its anticancer activity has not yet been fully studied. In the present research, cells from seven different cancer types were used to study the anticancer effect, and IPM713 was found to inhibit the colorectal cancer cell line HCT116 most significantly, with a half maximal inhibitory concentration (IC) of 1.7 μM. The mechanisms by which IPM713 exerts anti-colorectal cancer activity were studied. IPM713 blocked the cell cycle in G0/G1 phase and induced apoptosis by suppressing the PI3K/AKT/mTOR axis. In addition, an acute toxicity test showed that the median lethal dose (LD) was above 5000 mg/kg. The findings of this research suggest that IPM713 can interfere with the PI3K/AKT/mTOR signaling pathway and might be a potential therapeutic candidate for the treatment of colorectal cancer.
Shujian Hu, Wantong Ma, Junyi Wang, Zhongkun Zhou, Yunhao Ma, Rentao Zhang, Kangjia Du, Hao Zhang, Mengze Sun, Xinrong Jiang, Hongyuan Tu, Xiaoliang Tang, Xiaojun Yao, Peng Chen

1089 related Products with: Synthesis and biological activity of 1H-imidazo[4,5-f][1,10]phenanthroline as a potential antitumor agent with PI3K/AKT/mTOR signaling.

10 plates100 assays100 assays100 assays1 kit 96 Tests 100 assays100Tests500 assays

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#34559176   2021/09/24 To Up

Investigation of alkali and alkaline earth solvation structures in tetraglyme solvent.

This study compares molecular calculations performed with molecular and periodic codes through an investigation of the solvation structures of alkali and alkaline earth metal ions in tetraglyme solution. The two codes are able to produce equivalent structural and energetic information at the same level of theory, and in the presence of the implicit solvation model or not. This comparison reveals that molecular optimisations can be performed with periodic codes and used directly as input models for interface or electrochemistry calculations in order to preserve the solvent-solute interaction and the cavitation energy. By a rigorous comparison, we have demonstrated that equivalent energetic values can be obtained with the conventional PBE-D3 and the newly developed SCAN-rVV10 functionals. Nevertheless, as far as the vibrational features are concerned and when the molecule possesses a highly conjugated system, the SCAN-rVV10 functional is required to describe the vibrational modes properly. The computed IR/Raman spectra can thus be used as essential information to determine the first solvation shell of metal ions in glyme-based solutions. In tetraglyme solution, the alkali and alkaline earth metal ions exhibit a diverse solvation structure. Small ions like Li and Mg tend to adopt a coordination number of five or six, while larger ions, Na, K, and Ca, prefer an eight-coordinated environment, and the metal-ligand interaction increases in the order K-O < Na-O < Li-O < Ca-O < Mg-O. The solvation spheres play a significant role in the stability and the reactivity of the solvated ions, and can thus be used as input models to construct the solvation structure in more sophisticated electrolytes, such as polyethylene oxide, or perform electrochemical calculations.
L H B Nguyen, T Picard, N Sergent, C Raynaud, J-S Filhol, M-L Doublet

1307 related Products with: Investigation of alkali and alkaline earth solvation structures in tetraglyme solvent.

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#34559029   2021/09/24 To Up

An endophytic fungus, conjugated with C60 fullerenefor its potential antimitotic, anti-inflammatory, anticancer and photodegradation activities.

In the present study, endophytic fungus, isolated from was used to analyze phytochemical studies by qualitative and GC-MS methods. The endophytic fungus yielded novel compound penisimplicissin identified through GC-MS studies. conjugated with C60 fullerene nanoparticles (Ps-FNPs) were verified using UV-vis spectra, XRD, FTIR, DLS, EDX and SEM. Ps-FNPs was confirmed using UV-visible spectra with a peak at 260 nm. The IR bands were recorded at 2085, 1428, 1181, 661, 652, 644, 628, and 604 cm. The Ps-FNPs treated cells showed a nucleolar shrinkage and cell arrest at prophase, binuclear and multinucleolar cells, a chromosomal bridge and diversion at anaphase was observed, whereas, chromosomal fragment and abnormal distribution at metaphase stage. The Ps-FNPs exhibited a noteworthy anticancer activity on lung cancer cell line H1975 through cytotoxicity. The cytotoxicity was induced by increasing caspase-3, 7, and 9 activities and also showed highest inhibition in xanthine oxidase and COX-II assay proved good anti-inflammatory activity. Ps-FNPs have been extensively studied for photocatalytic activity test against Rhodamine B, Methylene blue and nigrosine showed potential dye degradation in the presence of sunlight proved to be novel photocatalysts. With all the results recorded, Ps-FNPs also have a synergetic effect having on anti-mitotic, anticancer, anti-inflammation potential and photocatalytic degradation of dyes. Hence, the compound penisimplicissin present in endophytic fungus, conjugated for C60 fullerene nanoparticles could be one of the potent nano-drug formulations. Thus, the present study gives a clear idea of the multifaceted therapeutic and photocatalytic applications.
Govindappa M, A Vishaka, B S Akshatha, Dimple Popli, N Sunayana, C Srinivas, Arivalagan Pugazhendhi, Vinay B Raghavendra

2333 related Products with: An endophytic fungus, conjugated with C60 fullerenefor its potential antimitotic, anti-inflammatory, anticancer and photodegradation activities.

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#34558652   2021/09/24 To Up

Bile reflux and hypopharyngeal cancer (Review).

Laryngopharyngeal reflux, a variant of gastroesophageal reflux disease, has been considered a risk factor in the development of hypopharyngeal cancer. Bile acids are frequently present in the gastroesophageal refluxate and their effect has been associated with inflammatory and neoplastic changes in the upper aerodigestive tract. Recent and studies have provided direct evidence of the role of acidic bile refluxate in hypopharyngeal carcinogenesis and documented the crucial role of NF‑κB as a key mediator of early oncogenic molecular events in this process and also suggested a contribution of STAT3. Acidic bile can cause premalignant changes and invasive squamous cell cancer in the affected hypopharynx accompanied by DNA damage, elevated p53 expression and oncogenic mRNA and microRNA alterations, previously linked to head and neck cancer. Weakly acidic bile can also increase the risk for hypopharyngeal carcinogenesis by inducing DNA damage, exerting anti‑apoptotic effects and causing precancerous lesions. The most important findings that strongly support bile reflux as an independent risk factor for hypopharyngeal cancer are presented in the current review and the underlying mechanisms are provided.
Dimitra P Vageli, Sotirios G Doukas, Panagiotis G Doukas, Benjamin L Judson

2289 related Products with: Bile reflux and hypopharyngeal cancer (Review).

200ug600 Tests / KitEach

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