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Expression and purification of affinity-tagged variants of the photochemical reaction center from Heliobacterium modesticaldum.

The heliobacterial photochemical reaction center (HbRC) from the chlorophototrophic Firmicutes bacterium Heliobacterium modesticaldum is the only homodimeric type I RC whose structure is known. Using genetic techniques recently established in our lab, we have developed a rapid heterologous expression system for the HbRC core polypeptide PshA. Our system relies on rescue of the non-chlorophototrophic ∆pshA::cbp2p-aph3 strain of Hbt. modesticaldum by expression of a heterologous pshA gene from a replicating shuttle vector. In addition, we constructed two tagged variants of PshA, one with an N-terminal octahistidine tag and one with an internal hexahistidine tag, which facilitate rapid purification of pure, active HbRC cores in milligram quantities. We constructed a suite of shuttle vectors bearing untagged or tagged versions of pshA driven by various promoters. Surprisingly, we found that the eno and gapDH_2 promoters from Clostridium thermocellum drive better expression of pshA than fragments of DNA derived from the region upstream of the pshA locus on the Hbt. modesticaldum genome. This "pshA rescue" strategy also provided a useful window into how Hbt. modesticaldum regulates pigment synthesis and growth rate when chlorophototrophic output decreases.

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AccuRapid™ Cell Free Pr Recombinant Mn SOD (Human ExiPrep His tagged Protei Ofloxacin CAS Number [824 baculoCOMPLETE protein ex AccuPrep His tagged Prote pCMVLuxA Mammalian LuxA E Thermal Shaker with cooli PAR Polyclonal Antibody A Androgen Receptor (Ab 650 Antibodies Purification PDE4D Assay Kit

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The spatial distribution of total mercury in sediments in the Yatsushiro Sea, Japan.

The Yatsushiro Sea in Japan is contaminated with mercury in wastewater discharge from the Chisso Company, which produced acetaldehyde from 1932 onwards. In this study, we investigated the current spatial distribution of total mercury (T-Hg) in sediments, both at the surface and at different depths, using 43 sediment cores and 22 surface sediment samples collected from the Yatsushiro Sea in August 2017. Altogether, 821 surface and core samples were analyzed for their T-Hg concentrations. Most of the mercury that was discharged from Chisso remained either in, or around the exit of, Minamata Bay, but some had been transported from Minamata Bay to the Nagashima Sea. We estimated that almost 51 and 6 tons of mercury had accumulated in the Yatsushiro and the Amakusa Sea areas, respectively. The amount of acetaldehyde produced in Chisso over time was correlated with the T-Hg concentrations in the sediments from the Yatsushiro Sea.

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FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss Thermal Shaker with cooli FDA Standard Frozen Tissu MultiGene Gradient therm Cell Meter™ Fluorimetri ELISA Mouse , Interleukin Cell Meter™ Fluorimetri

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Mediterranean Coastal Lagoons: The Importance of Monitoring in Sediments the Biochemical Composition of Organic Matter.

Transitional water ecosystems are targeted by the European Union (EU) Water Framework Directive (WFD, CE 2000/60) monitoring programs in coastal zones. Concerning sediments, activities performed for the WFD focus on a few variables concerning the biochemical composition of organic matter. Our research reports the effects of oxygen availability on the biochemical composition of organic matter in sediments to highlight levels of targeted variables in time and, according to the depth of sediment layer, both under oxygenated and anoxic conditions in a mesocosm study on sediment cores. Results provide evidence that tested factors of interest (i.e., disturbance type, oxygenic anoxic conditions; persistence time of disturbance, 0-14 days; penetration through sedimentary layers, 0-10 cm depth) are able to significantly affect the biochemical composition of organic matter in sediments. Large part of the variables considered in this study (total organic carbon (TOC), total phosphorous (TP), total sulphur (TS), Fe, carbohydrates (CHO), total proteins (PRT), biopolymeric carbon (BPC), chlorophyll-a (Chl-) are significantly affected and correlated to the oxygenation levels and could be good early indicators of important changes of environmental conditions. Monitoring activities performed under WFD guidelines and management strategies of Mediterranean coastal lagoon ecosystems shall include the biochemical composition of organic matter in sediment to provide an exhaustive picture of such dynamic ecosystems.

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Silicon-Core Carbon-Shell Nanoparticles for Lithium-ion Batteries: Rational Comparison Between Amorphous and Graphitic Carbon Coatings.

Silicon-core carbon-shell nanoparticles have been widely studied as promising candidates for the replacement of graphite in commercial lithium-ion batteries. Over more than 10 years of R&D, the many groups actively working in this field have proposed a profusion of distinctive nanomaterial designs. This broad variety makes it extremely challenging to establish mechanistic insight into how fundamental material structure and properties affect battery performance. In particular, the interplay between the character of the carbon encapsulation layer and the electrochemical performance of the composite is still poorly understood. In this work, we aim to address this lack of knowledge through the development of a modified chemical vapor deposition approach that enables precise control of the degree of graphitization of the carbon coating. We provide a comparison between core-shell structures maintaining identical silicon cores with different types of carbon shells, i.e. graphitic carbon and amorphous carbon. A highly graphitic carbon layer is not only characterized by higher electrical conductivity but markedly favors the transport of lithium ions into the silicon core with respect to an amorphous one. This advantageous property confers better cycling stability to the composite material. We also demonstrate that the graphitic-carbon-coated particles display excellent electrochemical performance even when used as a simple "drop-in" additive in graphite-dominant anodes for current generation Li-ion batteries. Replacement of 10% by weight of graphite in the electrode composition results in an increase of 60% in the storage capacity, with a first cycle Coulombic efficiency of 91% and capacity retention over 100 cycles of 81%.

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Multiple lung carcinoma ( Lymphoma array, together Lithium carbonate CAS Num Breast invasive ductal ca Carbon Dioxide, 10 x 6 ml Mid advanced stage ovary High density tissue array rac-N-Benzyl-N-[2-hydroxy Human breast invasive duc Non small cell lung carci Prostate cancer, PIN (pro Multiple organ condyloma

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Novel compounds of hybrid structure pyridazinone-coumarin as potent inhibitors of platelet aggregation.

The current limitations of antiplatelet therapy promote the search for new antithrombotic agents. Here we describe novel platelet aggregation inhibitors that combine pyridazinone and coumarin scaffolds in their structure. The target compounds were synthesized in good yield from maleic anhydride, following a multistep strategy. The studies demonstrated significant antiplatelet activity in many of these compounds, with IC values in the low micromolar range, revealing that the activity was affected by the substitution pattern of the two selected cores. Additional studies point out their effect as inhibitors of glycoprotein (Gp) IIb/IIIa activation. This novel hybrid structure can be considered a good prototype for the development of potent platelet aggregation inhibitors.

1874 related Products with: Novel compounds of hybrid structure pyridazinone-coumarin as potent inhibitors of platelet aggregation.

Cell Meter™ NIR Mitocho Screen Quest™ Membrane 7-(Diethylamino)coumarin- EnzyChrom™ Kinase Assay Ofloxacin CAS Number [824 Cell Meter™ Mitochondri Cell Meter™ JC 10 Mitoc Screen Quest™ Membrane Cell Meter™ NIR Mitocho Screen Quest™ Membrane Cell Meter™ JC 10 Mitoc Screen Quest™ Membrane

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Clinical fitting of a cast metal post and core obtained by means of an intraoral optical scanning (IOS) and digital workflow.

Customization of post-and-cores using computer-aided-design and computer-aided-manufacturing (CAD-CAM) requires the scanning of a pattern and the subsequent digital design. This case report describes the production of a CAD-CAM customized post-and-core designed from an intraoral scan and milled from a metal block. The use of an intraoral scanner (IOS) for post-endodontic rehabilitation could lead to a faster and more efficient CAD-CAM customized post-and-core realization. The use of a high resistance material such as metal is paramount in cases with high loss of coronal structure. The patient has been treated with bisphosphonate (BP) for years. The risk of osteonecrosis of the jaw after extraction was high.

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Liver cancer tissue array ∆1-Androstene-3α,17β- Breast cancer and matched Brain tumor tissue array, Endometrial cancer tissue Mesothelioma tissue array Androgen Receptor (Ab-650 Colon cancer tissue array Androgen Receptor (Ab 650 Stomach cancer tissue arr Colon cancer tissue array Lung carcinoma tissue arr

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Structure-based inhibitors halt prion-like seeding by Alzheimer's disease- and tauopathy-derived brain tissue samples.

In Alzheimer's disease (AD) and tauopathies, tau aggregation accompanies progressive neurodegeneration. Aggregated tau appears to spread between adjacent neurons and adjacent brain regions by prion-like seeding. Hence, inhibitors of this seeding offer a possible route to managing tauopathies. Here, we report the 1.0 Å resolution micro-electron diffraction structure of an aggregation-prone segment of tau with the sequence SVQIVY, present in the cores of patient-derived fibrils from AD and tauopathies. This structure illuminates how distinct interfaces of the parent segment, containing the sequence VQIVYK, foster the formation of distinct structures. Peptide-based fibril-capping inhibitors designed to target the two VQIVYK interfaces blocked proteopathic seeding by patient-derived fibrils. These VQIVYK inhibitors add to a panel of tau-capping inhibitors that targets specific polymorphs of recombinant and patient-derived tau fibrils. Inhibition of seeding initiated by brain tissue extracts differed among donors with different tauopathies, suggesting that particular fibril polymorphs of tau are associated with certain tauopathies. Donors with progressive supranuclear palsy (PSP) exhibited more variation in inhibitor sensitivity, suggesting that fibrils from these donors were more polymorphic and potentially vary within individual donor brains. Our results suggest that a subset of inhibitors from our panel could be specific for particular disease-associated polymorphs, whereas inhibitors that blocked seeding by extracts from all of the tauopathies tested could be used to broadly inhibit seeding by multiple disease-specific tau polymorphs. Moreover, we show that tau-capping inhibitors can be transiently expressed in HEK293 tau biosensor cells, indicating that nucleic acid-based vectors can be used for inhibitor delivery.

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Brain disease spectrum (b Beta Amyloid (40) ELISA K Beta Amyloid (42) ELISA K Beta Amyloid (1 40) ELISA Beta Amyloid (1 40) ELISA Ureter and urethra diseas  FitAmp Paraffin Tissue Breast disease spectrum t Peripheral nerve disease Digestive system disease Vulval disease spectrum ( Brains of multiple specie

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One-Pot and Postsynthetic Phenol-Thermal Synthesis toward Highly Stable Titanium-Oxo Clusters.

The synthetic approach plays a crucial role for the exploration and optimization of functional materials. As the molecular models of titanium dioxide, polyoxo-titanium clusters have undergone rapid development over past decade. Unfortunately, many of them are unstable, especially in aqueous environments, greatly limiting their applications in catalysis and environmental fields. In this work, we report a novel phenol-thermal approach toward the construction of highly stable polyoxo-titanium clusters. In addition to the traditional one-pot procedure, the phenol-thermal synthesis can also be used as a postsynthetic pathway to modify the alcohol terminated titanium-oxo clusters. During the modification in phenol, Ti-O core structures consisting entirely of 6-coordinated Ti centers can be retained. Nevertheless, isopropanol terminated 5-coordinated Ti centers are not stable and reconstructed to 6-coordinated Ti centers during the phenol-thermal modification to form new Ti-O clusters. Physical attribute studies confirm that the obtained phenolic clusters generally display much better stability and stronger visible light absorption than isopropanol stabilized clusters with identical or similar cores. Therefore, phenol can not only offer a suitable solution environment for the construction of new cluster structures but also provide robust protection for the cluster cores and also an efficient method to enhance their visible light responses.

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Association between time to treatment and functional outcomes according to DWI-ASPECTS in endovascular stroke therapy.

The rate at which the chance of a good outcome of endovascular stroke therapy (EVT) decays with time when eligible patients are selected by baseline DWI-MRI and whether ischaemic core size affects this rate remain to be investigated.

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Neutron spin echo monitoring of segmental-like diffusion of water confined in the cores of carbon nanotubes.

Following the stream of increasing scientific interest in condensed-matter systems under ultra-hydrophobic confinement, the present work reports the first incoherent neutron spin echo assessment of the dynamics of water axially confined inside single-wall carbon nanotubes of diameter d ∼ 1.4 nm. At the time scale of nanoseconds, two water populations are retrieved, whose relative proportion matches the one expected for a concentric shell + chain arrangement with cylindrical symmetry. The time dependence of the mean square displacement related to the external component is found to be subdiffusive, with peculiar resemblance to segmental diffusion typical of entangled polymeric systems.

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Ofloxacin CAS Number [824 FDA Standard Frozen Tissu Multiple organ tumor tiss FDA Standard Frozen Tissu Stomach adenocarcinoma ti Liver cancer test tissue Tissue array of gastric d Colon cancer tissue array Prostate cancer tissue ar Prostate cancer test tiss Breast invasive ductal ca Liver cancer and normal t

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